Off Target, but Sequence-Specific, shRNA-Associated Trans-Activation of Promoter Reporters in Transient Transfection Assays

Transient transfection promoter reporter assays are commonly used in the study of transcriptional regulation, and can be used to define and characterize both cis-acting regulatory sequences and trans-acting factors. In the process of using a variety of reporter assays designed to study regulation of...

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Veröffentlicht in:	PloS one 2016-12, Vol.11 (12), p.e0167867-e0167867
Hauptverfasser:	Masuda, Tomohiro, Wan, Jun, Yerrabelli, Anitha, Berlinicke, Cindy, Kallman, Alyssa, Qian, Jiang, Zack, Donald J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Assaying Biology and life sciences Chlorocebus aethiops Cloning COS Cells Data interpretation Design DNA microarrays Flow Cytometry Gene expression Gene regulation Genes Genetic aspects Genetic regulation HEK293 Cells Human health and pathology Humans Interferon Kinases Life Sciences Medicine MicroRNA MicroRNAs miRNA Neurosciences Photoreceptors Physiological aspects Plasmids Polymerase Chain Reaction Promoter Regions, Genetic - genetics Protein arrays Regulatory sequences Research and Analysis Methods Rhodopsin Rhodopsin - genetics Ribonucleic acid RNA RNA, Small Interfering - genetics RNA-mediated interference Target recognition Transcription Transcription factors Transcriptional Activation - genetics Transfection
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