A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability
This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by...
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| Veröffentlicht in: | PloS one 2016-12, Vol.11 (12), p.e0167863-e0167863 |
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| creator | Hasan, Md Mehedi Alam, Mohammad Wajih Wahid, Khan A Miah, Sayem Lukong, Kiven Erique |
| description | This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size. |
| doi_str_mv | 10.1371/journal.pone.0167863 |
| format | Article |
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The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0167863</identifier><identifier>PMID: 27977709</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biochemistry ; Biopsy ; Cancer ; Commercial off-the-shelf technology ; Computer and Information Sciences ; Computer engineering ; Construction costs ; Data buses ; Design and construction ; Digital imaging ; Endoscopy ; Engineering and Technology ; Excitation ; Flashlights ; Fluorescence ; Fluorescence microscopy ; Fluorescent Dyes ; Fluoroscopic imaging ; Gastroenterology ; Image transmission ; Laboratories ; Light sources ; Low cost ; Microscopes ; Microscopy ; Microscopy, Fluorescence - economics ; Microscopy, Fluorescence - instrumentation ; Optical properties ; Physical Sciences ; Real time ; Research and Analysis Methods ; Students ; Tuberculosis ; Tumor cells ; Weight reduction ; Zooming</subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0167863-e0167863</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Hasan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasan, Md Mehedi</au><au>Alam, Mohammad Wajih</au><au>Wahid, Khan A</au><au>Miah, Sayem</au><au>Lukong, Kiven Erique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-12-15</date><risdate>2016</risdate><volume>11</volume><issue>12</issue><spage>e0167863</spage><epage>e0167863</epage><pages>e0167863-e0167863</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. 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| subjects | Biochemistry Biopsy Cancer Commercial off-the-shelf technology Computer and Information Sciences Computer engineering Construction costs Data buses Design and construction Digital imaging Endoscopy Engineering and Technology Excitation Flashlights Fluorescence Fluorescence microscopy Fluorescent Dyes Fluoroscopic imaging Gastroenterology Image transmission Laboratories Light sources Low cost Microscopes Microscopy Microscopy, Fluorescence - economics Microscopy, Fluorescence - instrumentation Optical properties Physical Sciences Real time Research and Analysis Methods Students Tuberculosis Tumor cells Weight reduction Zooming |
| title | A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability |
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