Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea
Human papillomavirus (HPV) 52 is a carcinogenic, high-risk genotype frequently detected in cervical cancer cases from East Asia, including Korea. Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences we...
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| creator | Choi, Youn Jin Ki, Eun Young Zhang, Chuqing Ho, Wendy C S Lee, Sung-Jong Jeong, Min Jin Chan, Paul K S Park, Jong Sup |
| description | Human papillomavirus (HPV) 52 is a carcinogenic, high-risk genotype frequently detected in cervical cancer cases from East Asia, including Korea.
Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences were obtained by polymerase chain reaction (PCR)-based sequencing and analyzed using Seq-Scape software, and phylogenetic trees were constructed using MEGA6 software.
Of the 91 cervical samples, 40 were normal, 22 were low-grade lesions, 21 were high-grade lesions and 7 were squamous cell carcinomas. Four HPV52 variant lineages (A, B, C and D) were identified. Lineage B was the most frequently detected lineage, followed by lineage C. By analyzing the two most frequently detected lineages (B and C), we found that distinct variations existed in each lineage. We also found that a lineage B-specific mutation K93R (A379G) was associated with an increased risk of cervical neoplasia.
To our knowledge, we are the first to reveal the predominance of the HPV52 lineages, B and C, in Korea. We also found these lineages harbored distinct genetic alterations that may affect oncogenicity. Our findings increase our understanding on the heterogeneity of HPV52 variants, and may be useful for the development of new diagnostic assays and therapeutic vaccines. |
| doi_str_mv | 10.1371/journal.pone.0168178 |
| format | Article |
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Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences were obtained by polymerase chain reaction (PCR)-based sequencing and analyzed using Seq-Scape software, and phylogenetic trees were constructed using MEGA6 software.
Of the 91 cervical samples, 40 were normal, 22 were low-grade lesions, 21 were high-grade lesions and 7 were squamous cell carcinomas. Four HPV52 variant lineages (A, B, C and D) were identified. Lineage B was the most frequently detected lineage, followed by lineage C. By analyzing the two most frequently detected lineages (B and C), we found that distinct variations existed in each lineage. We also found that a lineage B-specific mutation K93R (A379G) was associated with an increased risk of cervical neoplasia.
To our knowledge, we are the first to reveal the predominance of the HPV52 lineages, B and C, in Korea. We also found these lineages harbored distinct genetic alterations that may affect oncogenicity. Our findings increase our understanding on the heterogeneity of HPV52 variants, and may be useful for the development of new diagnostic assays and therapeutic vaccines.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0168178</identifier><identifier>PMID: 27977741</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology and Life Sciences ; Carcinogens ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - virology ; Cell cycle ; Cellular biology ; Cervical cancer ; Cervix ; Computer programs ; Deoxyribonucleic acid ; Departments ; Diagnostic systems ; Distribution ; DNA ; DNA polymerase ; DNA, Viral ; Female ; Genetic aspects ; Genetic Variation ; Genotype & phenotype ; Genotypes ; Gynecology ; Health aspects ; Health risks ; Human papillomavirus ; Humans ; Lesions ; Medicine ; Medicine and Health Sciences ; Mutation ; Obstetrics ; Papillomaviridae ; Papillomaviridae - genetics ; Papillomavirus ; Papillomavirus infections ; Papillomavirus Infections - pathology ; Papillomavirus Infections - virology ; Phylogeny ; Polymerase chain reaction ; Republic of Korea ; Research and Analysis Methods ; Risk ; Sequence Analysis, DNA ; Squamous cell carcinoma ; Thermal cycling ; Uterine Cervical Neoplasms - pathology ; Uterine Cervical Neoplasms - virology ; Vaccines</subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0168178</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Choi et al 2016 Choi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-7ea9066fa3dc6f1ce403138ec935b034cb515b6c677aca545520b041f9ee7c8b3</citedby><cites>FETCH-LOGICAL-c791t-7ea9066fa3dc6f1ce403138ec935b034cb515b6c677aca545520b041f9ee7c8b3</cites><orcidid>0000-0003-4086-4885</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5158036/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5158036/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2104,2930,23873,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27977741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Liu, Xuefeng</contributor><creatorcontrib>Choi, Youn Jin</creatorcontrib><creatorcontrib>Ki, Eun Young</creatorcontrib><creatorcontrib>Zhang, Chuqing</creatorcontrib><creatorcontrib>Ho, Wendy C S</creatorcontrib><creatorcontrib>Lee, Sung-Jong</creatorcontrib><creatorcontrib>Jeong, Min Jin</creatorcontrib><creatorcontrib>Chan, Paul K S</creatorcontrib><creatorcontrib>Park, Jong Sup</creatorcontrib><title>Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Human papillomavirus (HPV) 52 is a carcinogenic, high-risk genotype frequently detected in cervical cancer cases from East Asia, including Korea.
Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences were obtained by polymerase chain reaction (PCR)-based sequencing and analyzed using Seq-Scape software, and phylogenetic trees were constructed using MEGA6 software.
Of the 91 cervical samples, 40 were normal, 22 were low-grade lesions, 21 were high-grade lesions and 7 were squamous cell carcinomas. Four HPV52 variant lineages (A, B, C and D) were identified. Lineage B was the most frequently detected lineage, followed by lineage C. By analyzing the two most frequently detected lineages (B and C), we found that distinct variations existed in each lineage. We also found that a lineage B-specific mutation K93R (A379G) was associated with an increased risk of cervical neoplasia.
To our knowledge, we are the first to reveal the predominance of the HPV52 lineages, B and C, in Korea. We also found these lineages harbored distinct genetic alterations that may affect oncogenicity. Our findings increase our understanding on the heterogeneity of HPV52 variants, and may be useful for the development of new diagnostic assays and therapeutic vaccines.</description><subject>Biology and Life Sciences</subject><subject>Carcinogens</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - virology</subject><subject>Cell cycle</subject><subject>Cellular biology</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Computer programs</subject><subject>Deoxyribonucleic acid</subject><subject>Departments</subject><subject>Diagnostic systems</subject><subject>Distribution</subject><subject>DNA</subject><subject>DNA polymerase</subject><subject>DNA, Viral</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Genetic Variation</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Gynecology</subject><subject>Health aspects</subject><subject>Health risks</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Lesions</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mutation</subject><subject>Obstetrics</subject><subject>Papillomaviridae</subject><subject>Papillomaviridae - 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pathology</topic><topic>Carcinoma, Squamous Cell - virology</topic><topic>Cell cycle</topic><topic>Cellular biology</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Computer programs</topic><topic>Deoxyribonucleic acid</topic><topic>Departments</topic><topic>Diagnostic systems</topic><topic>Distribution</topic><topic>DNA</topic><topic>DNA polymerase</topic><topic>DNA, Viral</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Genetic Variation</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Gynecology</topic><topic>Health aspects</topic><topic>Health risks</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Lesions</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Mutation</topic><topic>Obstetrics</topic><topic>Papillomaviridae</topic><topic>Papillomaviridae - genetics</topic><topic>Papillomavirus</topic><topic>Papillomavirus infections</topic><topic>Papillomavirus Infections - pathology</topic><topic>Papillomavirus Infections - virology</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Republic of Korea</topic><topic>Research and Analysis Methods</topic><topic>Risk</topic><topic>Sequence Analysis, DNA</topic><topic>Squamous cell carcinoma</topic><topic>Thermal cycling</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Uterine Cervical Neoplasms - virology</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Youn Jin</creatorcontrib><creatorcontrib>Ki, Eun Young</creatorcontrib><creatorcontrib>Zhang, Chuqing</creatorcontrib><creatorcontrib>Ho, Wendy C S</creatorcontrib><creatorcontrib>Lee, Sung-Jong</creatorcontrib><creatorcontrib>Jeong, Min Jin</creatorcontrib><creatorcontrib>Chan, Paul K S</creatorcontrib><creatorcontrib>Park, Jong Sup</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences were obtained by polymerase chain reaction (PCR)-based sequencing and analyzed using Seq-Scape software, and phylogenetic trees were constructed using MEGA6 software.
Of the 91 cervical samples, 40 were normal, 22 were low-grade lesions, 21 were high-grade lesions and 7 were squamous cell carcinomas. Four HPV52 variant lineages (A, B, C and D) were identified. Lineage B was the most frequently detected lineage, followed by lineage C. By analyzing the two most frequently detected lineages (B and C), we found that distinct variations existed in each lineage. We also found that a lineage B-specific mutation K93R (A379G) was associated with an increased risk of cervical neoplasia.
To our knowledge, we are the first to reveal the predominance of the HPV52 lineages, B and C, in Korea. We also found these lineages harbored distinct genetic alterations that may affect oncogenicity. Our findings increase our understanding on the heterogeneity of HPV52 variants, and may be useful for the development of new diagnostic assays and therapeutic vaccines.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27977741</pmid><doi>10.1371/journal.pone.0168178</doi><tpages>e0168178</tpages><orcidid>https://orcid.org/0000-0003-4086-4885</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2016-12, Vol.11 (12), p.e0168178 |
| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biology and Life Sciences Carcinogens Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - virology Cell cycle Cellular biology Cervical cancer Cervix Computer programs Deoxyribonucleic acid Departments Diagnostic systems Distribution DNA DNA polymerase DNA, Viral Female Genetic aspects Genetic Variation Genotype & phenotype Genotypes Gynecology Health aspects Health risks Human papillomavirus Humans Lesions Medicine Medicine and Health Sciences Mutation Obstetrics Papillomaviridae Papillomaviridae - genetics Papillomavirus Papillomavirus infections Papillomavirus Infections - pathology Papillomavirus Infections - virology Phylogeny Polymerase chain reaction Republic of Korea Research and Analysis Methods Risk Sequence Analysis, DNA Squamous cell carcinoma Thermal cycling Uterine Cervical Neoplasms - pathology Uterine Cervical Neoplasms - virology Vaccines |
| title | Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T07%3A33%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20Sequence%20Variation%20and%20Risk%20Association%20of%20Human%20Papillomavirus%2052%20Variants%20Circulating%20in%20Korea&rft.jtitle=PloS%20one&rft.au=Choi,%20Youn%20Jin&rft.date=2016-12-15&rft.volume=11&rft.issue=12&rft.spage=e0168178&rft.pages=e0168178-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0168178&rft_dat=%3Cgale_plos_%3EA474010341%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1850130128&rft_id=info:pmid/27977741&rft_galeid=A474010341&rft_doaj_id=oai_doaj_org_article_a01c3fc446f0432280d664259c33124a&rfr_iscdi=true |