Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth

The endoplasmic reticulum (ER), including the nuclear envelope, is a continuous and intricate membrane-bound organelle responsible for various cellular functions. In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in ne...

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Veröffentlicht in:PloS one 2016-12, Vol.11 (12), p.e0167985-e0167985
Hauptverfasser: Watanabe, Keisuke, Bizen, Norihisa, Sato, Noboru, Takebayashi, Hirohide
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Bizen, Norihisa
Sato, Noboru
Takebayashi, Hirohide
description The endoplasmic reticulum (ER), including the nuclear envelope, is a continuous and intricate membrane-bound organelle responsible for various cellular functions. In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in neuronal development, such as neuronal migration and axonal outgrowth. However, the regulation of neural development by ER-localized proteins is not fully understood. We previously reported that the multi-transmembrane protein Dpy19L1 is required for neuronal migration in the developing mouse cerebral cortex. A Dpy19L family member, Dpy19L2, which is a causative gene for human Globozoospermia, is suggested to act as an anchor of the acrosome to the nuclear envelope. In this study, we found that the patterns of exogenous Dpy19L1 were partially coincident with the ER, including the nuclear envelope in COS-7 cells at the level of the light microscope. The reticular distribution of Dpy19L1 was disrupted by microtubule depolymerization that induces retraction of the ER. Furthermore, Dpy19L1 showed a similar distribution pattern with a ER marker protein in embryonic mouse cortical neurons. Finally, we showed that Dpy19L1 knockdown mediated by siRNA resulted in decreased neurite outgrowth in cultured neurons. These results indicate that transmembrane protein Dpy19L1 is localized to the ER membrane and regulates neurite extension during development.
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subjects Analysis
Animals
Axonogenesis
Axons
Biology and Life Sciences
Bipolar disorder
Cell division
Cell migration
Cells, Cultured
Cercopithecus aethiops
Cerebral cortex
Cerebral Cortex - cytology
Cerebral Cortex - metabolism
COS Cells
Cytoplasm
Dendrites
Depolymerization
Distribution patterns
Embryos
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
Female
Localization
Membrane proteins
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mice
Mice, Inbred ICR
Morphogenesis
Nervous system
Neurobiology
Neuronal Outgrowth
Neurons
Neurons - cytology
Neurons - metabolism
Neurosciences
Paralysis
Protein Transport
Proteins
Research and Analysis Methods
siRNA
Transmembrane proteins
title Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth
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