Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth
The endoplasmic reticulum (ER), including the nuclear envelope, is a continuous and intricate membrane-bound organelle responsible for various cellular functions. In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in ne...
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description | The endoplasmic reticulum (ER), including the nuclear envelope, is a continuous and intricate membrane-bound organelle responsible for various cellular functions. In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in neuronal development, such as neuronal migration and axonal outgrowth. However, the regulation of neural development by ER-localized proteins is not fully understood. We previously reported that the multi-transmembrane protein Dpy19L1 is required for neuronal migration in the developing mouse cerebral cortex. A Dpy19L family member, Dpy19L2, which is a causative gene for human Globozoospermia, is suggested to act as an anchor of the acrosome to the nuclear envelope. In this study, we found that the patterns of exogenous Dpy19L1 were partially coincident with the ER, including the nuclear envelope in COS-7 cells at the level of the light microscope. The reticular distribution of Dpy19L1 was disrupted by microtubule depolymerization that induces retraction of the ER. Furthermore, Dpy19L1 showed a similar distribution pattern with a ER marker protein in embryonic mouse cortical neurons. Finally, we showed that Dpy19L1 knockdown mediated by siRNA resulted in decreased neurite outgrowth in cultured neurons. These results indicate that transmembrane protein Dpy19L1 is localized to the ER membrane and regulates neurite extension during development. |
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In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in neuronal development, such as neuronal migration and axonal outgrowth. However, the regulation of neural development by ER-localized proteins is not fully understood. We previously reported that the multi-transmembrane protein Dpy19L1 is required for neuronal migration in the developing mouse cerebral cortex. A Dpy19L family member, Dpy19L2, which is a causative gene for human Globozoospermia, is suggested to act as an anchor of the acrosome to the nuclear envelope. In this study, we found that the patterns of exogenous Dpy19L1 were partially coincident with the ER, including the nuclear envelope in COS-7 cells at the level of the light microscope. The reticular distribution of Dpy19L1 was disrupted by microtubule depolymerization that induces retraction of the ER. Furthermore, Dpy19L1 showed a similar distribution pattern with a ER marker protein in embryonic mouse cortical neurons. Finally, we showed that Dpy19L1 knockdown mediated by siRNA resulted in decreased neurite outgrowth in cultured neurons. These results indicate that transmembrane protein Dpy19L1 is localized to the ER membrane and regulates neurite extension during development.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0167985</identifier><identifier>PMID: 27959946</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Axonogenesis ; Axons ; Biology and Life Sciences ; Bipolar disorder ; Cell division ; Cell migration ; Cells, Cultured ; Cercopithecus aethiops ; Cerebral cortex ; Cerebral Cortex - cytology ; Cerebral Cortex - metabolism ; COS Cells ; Cytoplasm ; Dendrites ; Depolymerization ; Distribution patterns ; Embryos ; Endoplasmic reticulum ; Endoplasmic Reticulum - metabolism ; Female ; Localization ; Membrane proteins ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred ICR ; Morphogenesis ; Nervous system ; Neurobiology ; Neuronal Outgrowth ; Neurons ; Neurons - cytology ; Neurons - metabolism ; Neurosciences ; Paralysis ; Protein Transport ; Proteins ; Research and Analysis Methods ; siRNA ; Transmembrane proteins</subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0167985-e0167985</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Watanabe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Watanabe et al 2016 Watanabe et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-3a204993b214549b9cfc1c04ea3ad464b124d347e5126568e2130fbaa29fdced3</citedby><cites>FETCH-LOGICAL-c791t-3a204993b214549b9cfc1c04ea3ad464b124d347e5126568e2130fbaa29fdced3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154530/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154530/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27959946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Larijani, Banafshe</contributor><creatorcontrib>Watanabe, Keisuke</creatorcontrib><creatorcontrib>Bizen, Norihisa</creatorcontrib><creatorcontrib>Sato, Noboru</creatorcontrib><creatorcontrib>Takebayashi, Hirohide</creatorcontrib><title>Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The endoplasmic reticulum (ER), including the nuclear envelope, is a continuous and intricate membrane-bound organelle responsible for various cellular functions. In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in neuronal development, such as neuronal migration and axonal outgrowth. However, the regulation of neural development by ER-localized proteins is not fully understood. We previously reported that the multi-transmembrane protein Dpy19L1 is required for neuronal migration in the developing mouse cerebral cortex. A Dpy19L family member, Dpy19L2, which is a causative gene for human Globozoospermia, is suggested to act as an anchor of the acrosome to the nuclear envelope. In this study, we found that the patterns of exogenous Dpy19L1 were partially coincident with the ER, including the nuclear envelope in COS-7 cells at the level of the light microscope. The reticular distribution of Dpy19L1 was disrupted by microtubule depolymerization that induces retraction of the ER. Furthermore, Dpy19L1 showed a similar distribution pattern with a ER marker protein in embryonic mouse cortical neurons. Finally, we showed that Dpy19L1 knockdown mediated by siRNA resulted in decreased neurite outgrowth in cultured neurons. These results indicate that transmembrane protein Dpy19L1 is localized to the ER membrane and regulates neurite extension during development.</description><subject>Analysis</subject><subject>Animals</subject><subject>Axonogenesis</subject><subject>Axons</subject><subject>Biology and Life Sciences</subject><subject>Bipolar disorder</subject><subject>Cell division</subject><subject>Cell migration</subject><subject>Cells, Cultured</subject><subject>Cercopithecus aethiops</subject><subject>Cerebral cortex</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - metabolism</subject><subject>COS Cells</subject><subject>Cytoplasm</subject><subject>Dendrites</subject><subject>Depolymerization</subject><subject>Distribution patterns</subject><subject>Embryos</subject><subject>Endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Female</subject><subject>Localization</subject><subject>Membrane proteins</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Morphogenesis</subject><subject>Nervous system</subject><subject>Neurobiology</subject><subject>Neuronal Outgrowth</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Neurosciences</subject><subject>Paralysis</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>siRNA</subject><subject>Transmembrane proteins</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk19v0zAUxSMEYmPwDRBEQkLw0OL_iV8mTWNApYqiMXi1HMdpPTlxZzvA-PQ4azY1aA9THhw5v3NyfK9vlr2EYA5xAT9cut530s63rtNzAFnBS_ooO4QcoxlDAD_eez_InoVwCQDFJWNPswNUcMo5YYeZOOtqt7UytEbl5zoa1du-nS2dktb81XV-4WUXWt1WadX5N--iNl3-cXsN-RLmi5BEV73xiWycz7_q3puo81Uf1979jpvn2ZNG2qBfjOtR9uPT2cXpl9ly9XlxerKcqYLDOMMSAcI5rhAklPCKq0ZBBYiWWNaEkQoiUmNSaAoRo6zUCGLQVFIi3tRK1_goe73z3VoXxFibIGBJSgoYKEgiFjuidvJSbL1ppb8WThpxs-H8Wkifzm-1wJg2UNdyyJHCAMlrJBlTDZV1o5VMXsfj3_qq1SlAF720E9Ppl85sxNr9EhRSQjFIBu9GA--ueh2iaE1Q2tpUZNcPuWlqD8e4eAiKbpo_oG_-Q-8vxEitZTqr6RqXIqrBVJyQAhesBOVAze-h0lPrdFXSnWtM2p8I3k8EiYn6T1zLPgSx-H7-cHb1c8q-3WM3Wtq4Cc720bguTEGyA5V3IXjd3PUDAjGMzG01xDAyYhyZJHu138s70e2M4H-VUBGM</recordid><startdate>20161213</startdate><enddate>20161213</enddate><creator>Watanabe, Keisuke</creator><creator>Bizen, Norihisa</creator><creator>Sato, Noboru</creator><creator>Takebayashi, Hirohide</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20161213</creationdate><title>Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth</title><author>Watanabe, Keisuke ; Bizen, Norihisa ; Sato, Noboru ; Takebayashi, Hirohide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c791t-3a204993b214549b9cfc1c04ea3ad464b124d347e5126568e2130fbaa29fdced3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Axonogenesis</topic><topic>Axons</topic><topic>Biology and Life Sciences</topic><topic>Bipolar disorder</topic><topic>Cell division</topic><topic>Cell migration</topic><topic>Cells, Cultured</topic><topic>Cercopithecus aethiops</topic><topic>Cerebral cortex</topic><topic>Cerebral Cortex - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Keisuke</au><au>Bizen, Norihisa</au><au>Sato, Noboru</au><au>Takebayashi, Hirohide</au><au>Larijani, Banafshe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-12-13</date><risdate>2016</risdate><volume>11</volume><issue>12</issue><spage>e0167985</spage><epage>e0167985</epage><pages>e0167985-e0167985</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The endoplasmic reticulum (ER), including the nuclear envelope, is a continuous and intricate membrane-bound organelle responsible for various cellular functions. In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in neuronal development, such as neuronal migration and axonal outgrowth. However, the regulation of neural development by ER-localized proteins is not fully understood. We previously reported that the multi-transmembrane protein Dpy19L1 is required for neuronal migration in the developing mouse cerebral cortex. A Dpy19L family member, Dpy19L2, which is a causative gene for human Globozoospermia, is suggested to act as an anchor of the acrosome to the nuclear envelope. In this study, we found that the patterns of exogenous Dpy19L1 were partially coincident with the ER, including the nuclear envelope in COS-7 cells at the level of the light microscope. The reticular distribution of Dpy19L1 was disrupted by microtubule depolymerization that induces retraction of the ER. Furthermore, Dpy19L1 showed a similar distribution pattern with a ER marker protein in embryonic mouse cortical neurons. Finally, we showed that Dpy19L1 knockdown mediated by siRNA resulted in decreased neurite outgrowth in cultured neurons. These results indicate that transmembrane protein Dpy19L1 is localized to the ER membrane and regulates neurite extension during development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27959946</pmid><doi>10.1371/journal.pone.0167985</doi><tpages>e0167985</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Analysis Animals Axonogenesis Axons Biology and Life Sciences Bipolar disorder Cell division Cell migration Cells, Cultured Cercopithecus aethiops Cerebral cortex Cerebral Cortex - cytology Cerebral Cortex - metabolism COS Cells Cytoplasm Dendrites Depolymerization Distribution patterns Embryos Endoplasmic reticulum Endoplasmic Reticulum - metabolism Female Localization Membrane proteins Membrane Proteins - genetics Membrane Proteins - metabolism Mice Mice, Inbred ICR Morphogenesis Nervous system Neurobiology Neuronal Outgrowth Neurons Neurons - cytology Neurons - metabolism Neurosciences Paralysis Protein Transport Proteins Research and Analysis Methods siRNA Transmembrane proteins |
title | Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth |
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