High Expression of KIF20A Is Associated with Poor Overall Survival and Tumor Progression in Early-Stage Cervical Squamous Cell Carcinoma
The kinesin family member 20a (KIF20A) protein has been implicated in the development and progression of many human cancers; however, its precise function and role in cervical cancer remain largely unclear. This study aimed to investigate the expression profile and prognostic value of KIF20A in pati...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-12, Vol.11 (12), p.e0167449-e0167449 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0167449 |
|---|---|
| container_issue | 12 |
| container_start_page | e0167449 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Zhang, Weijing He, Weiling Shi, Yongjie Gu, Haifeng Li, Min Liu, Zhimin Feng, Yanling Zheng, Nianzhen Xie, Chuanmiao Zhang, Yanna |
| description | The kinesin family member 20a (KIF20A) protein has been implicated in the development and progression of many human cancers; however, its precise function and role in cervical cancer remain largely unclear. This study aimed to investigate the expression profile and prognostic value of KIF20A in patients with early-stage cervical squamous cell carcinoma.
We examined the mRNA and protein levels of KIF20A in eight cervical cancer cell lines and eight paired cervical cancer samples, compared with normal cervical epithelial cells and adjacent normal cervical tissues, respectively. Immunohistochemistry was performed to detect the expression of KIF20A in paraffin-embedded specimens from 169 early-stage cervical squamous cell carcinoma patients. Statistical analyses were applied to analyze the association between KIF20A expression and clinical variables, as well with patient survival.
The mRNA and protein expression levels of KIF20A were significantly elevated in cervical cancer cell lines and lesions compared with normal cells and corresponding normal tissues (P < 0.05). Immunohistochemistry analysis in 169 cervical cancer cases revealed that increased KIF20A expression was strongly associated with human papillomavirus (HPV) infection (P = 0.008), clinical stage (P = 0.001), tumor recurrence (P = 0.016), vital status (P < 0.001), the property of the surgical margin (P = 0.032), the lymphovascular space involvement (P = 0.014), and pelvic lymph node metastasis (P = 0.001). The overall survival and disease-free survival of patients with high levels of KIF20A expression were significantly poorer than those with low KIF20A expression. KIF20A was an independent survival prognostic factor, as evidenced by univariate and multivariate analysis.
Our results illustrate that elevated KIF20A expression correlates with HPV infection, clinical stage, tumor recurrence, lymphovascular space involvement, pelvic lymph node metastasis, and poor outcome in early-stage cervical squamous cell carcinoma patients. KIF20A aberrant expression is a novel independent unfavorable prognostic factor and may present a potential therapeutic target for cervical cancer. |
| doi_str_mv | 10.1371/journal.pone.0167449 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1848103550</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A473671645</galeid><doaj_id>oai_doaj_org_article_b59984ccef82441aa6d21b845e98bd88</doaj_id><sourcerecordid>A473671645</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-2309a1c3e533d4458d03ebccd92e255033ea03ab8e3380dcd38e131e7877e6893</originalsourceid><addsrcrecordid>eNqNk99u0zAUxiMEYqPwBggsISG4aPG_JPYNUlV1rGJSJzq4tVznNHWVxJ2dlO0NeGxc2k4t2sXkC1vHv_P5-LNPkrwleEBYTr6sXOcbXQ3WroEBJlnOuXyWnBPJaD-jmD0_Wp8lr0JYYZwykWUvkzOaS06kpOfJn0tbLtH4bu0hBOsa5Bbo--SC4iGaBDQMwRmrWyjQb9su0bVzHk034HVVoVnnN3ajK6SbAt10ddy69q48CNkGjbWv7vuzVpeARhBpE-nZbadr14UYiSIj7Y1tXK1fJy8WugrwZj_3kp8X45vRZf9q-m0yGl71TU7Ttk8ZlpoYBiljBeepKDCDuTGFpEDTFDMGGjM9F8CYwIUpmADCCOQizyETkvWS9zvddeWC2psYFBFcEMy2Cr1ksiMKp1dq7W2t_b1y2qp_AedLpX1rTQVqnkopuDGwEJRzonVWUDIXPAUp5oUQUevr_rRuXkNhoGmjdyeipzuNXarSbVRKUioojQKf9gLe3XYQWlXbYKJzuoFoYqw7lVxmXOCnoDTLMiJJRD_8hz5uxJ4qdbyrbRYulmi2omrIc5blJONppAaPUHEUUFsTP-fCxvhJwueThMi0cNeWugtBTWY_ns5Of52yH4_YJeiqXQZXdW38jeEU5DvQeBeCh8XDexCstr11cENte0vteyumvTt-y4ekQzOxv8LHHcU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1848103550</pqid></control><display><type>article</type><title>High Expression of KIF20A Is Associated with Poor Overall Survival and Tumor Progression in Early-Stage Cervical Squamous Cell Carcinoma</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Zhang, Weijing ; He, Weiling ; Shi, Yongjie ; Gu, Haifeng ; Li, Min ; Liu, Zhimin ; Feng, Yanling ; Zheng, Nianzhen ; Xie, Chuanmiao ; Zhang, Yanna</creator><contributor>Coleman, William B.</contributor><creatorcontrib>Zhang, Weijing ; He, Weiling ; Shi, Yongjie ; Gu, Haifeng ; Li, Min ; Liu, Zhimin ; Feng, Yanling ; Zheng, Nianzhen ; Xie, Chuanmiao ; Zhang, Yanna ; Coleman, William B.</creatorcontrib><description>The kinesin family member 20a (KIF20A) protein has been implicated in the development and progression of many human cancers; however, its precise function and role in cervical cancer remain largely unclear. This study aimed to investigate the expression profile and prognostic value of KIF20A in patients with early-stage cervical squamous cell carcinoma.
We examined the mRNA and protein levels of KIF20A in eight cervical cancer cell lines and eight paired cervical cancer samples, compared with normal cervical epithelial cells and adjacent normal cervical tissues, respectively. Immunohistochemistry was performed to detect the expression of KIF20A in paraffin-embedded specimens from 169 early-stage cervical squamous cell carcinoma patients. Statistical analyses were applied to analyze the association between KIF20A expression and clinical variables, as well with patient survival.
The mRNA and protein expression levels of KIF20A were significantly elevated in cervical cancer cell lines and lesions compared with normal cells and corresponding normal tissues (P < 0.05). Immunohistochemistry analysis in 169 cervical cancer cases revealed that increased KIF20A expression was strongly associated with human papillomavirus (HPV) infection (P = 0.008), clinical stage (P = 0.001), tumor recurrence (P = 0.016), vital status (P < 0.001), the property of the surgical margin (P = 0.032), the lymphovascular space involvement (P = 0.014), and pelvic lymph node metastasis (P = 0.001). The overall survival and disease-free survival of patients with high levels of KIF20A expression were significantly poorer than those with low KIF20A expression. KIF20A was an independent survival prognostic factor, as evidenced by univariate and multivariate analysis.
Our results illustrate that elevated KIF20A expression correlates with HPV infection, clinical stage, tumor recurrence, lymphovascular space involvement, pelvic lymph node metastasis, and poor outcome in early-stage cervical squamous cell carcinoma patients. KIF20A aberrant expression is a novel independent unfavorable prognostic factor and may present a potential therapeutic target for cervical cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0167449</identifier><identifier>PMID: 27941992</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aberration ; Adult ; Aged ; Antigens ; Biology and Life Sciences ; Biotechnology ; Breast cancer ; Cancer ; Cancer therapies ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - mortality ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - therapy ; Cell division ; Cell Line, Tumor ; Cell survival ; Cervical cancer ; Cervix ; Collaboration ; Combined Modality Therapy ; Comparative analysis ; Development and progression ; Disease Progression ; Epithelial cells ; Ethics ; Female ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Hospitals ; Human papillomavirus ; Humans ; Immunohistochemistry ; Infections ; Kaplan-Meier Estimate ; Kinesin ; Kinesin - genetics ; Laboratories ; Lesions ; Lymph ; Lymph nodes ; Lymphatic Metastasis ; Lymphatic system ; Medical prognosis ; Medicine ; Medicine and Health Sciences ; Melanoma ; Metastases ; Metastasis ; Middle Aged ; mRNA ; Multivariate analysis ; Neoplasm Grading ; Neoplasm Staging ; Oncology ; Pancreatic cancer ; Papillomaviridae ; Paraffin ; Patients ; Prognosis ; Proportional Hazards Models ; Proteins ; Radiation therapy ; Research and Analysis Methods ; Squamous cell carcinoma ; Statistical analysis ; Statistical methods ; Studies ; Surgery ; Survival ; Tissues ; Tumor cell lines ; Tumors ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - mortality ; Uterine Cervical Neoplasms - pathology ; Uterine Cervical Neoplasms - therapy ; Womens health</subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0167449-e0167449</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Zhang et al 2016 Zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-2309a1c3e533d4458d03ebccd92e255033ea03ab8e3380dcd38e131e7877e6893</citedby><cites>FETCH-LOGICAL-c725t-2309a1c3e533d4458d03ebccd92e255033ea03ab8e3380dcd38e131e7877e6893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5152822/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5152822/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27941992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Coleman, William B.</contributor><creatorcontrib>Zhang, Weijing</creatorcontrib><creatorcontrib>He, Weiling</creatorcontrib><creatorcontrib>Shi, Yongjie</creatorcontrib><creatorcontrib>Gu, Haifeng</creatorcontrib><creatorcontrib>Li, Min</creatorcontrib><creatorcontrib>Liu, Zhimin</creatorcontrib><creatorcontrib>Feng, Yanling</creatorcontrib><creatorcontrib>Zheng, Nianzhen</creatorcontrib><creatorcontrib>Xie, Chuanmiao</creatorcontrib><creatorcontrib>Zhang, Yanna</creatorcontrib><title>High Expression of KIF20A Is Associated with Poor Overall Survival and Tumor Progression in Early-Stage Cervical Squamous Cell Carcinoma</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The kinesin family member 20a (KIF20A) protein has been implicated in the development and progression of many human cancers; however, its precise function and role in cervical cancer remain largely unclear. This study aimed to investigate the expression profile and prognostic value of KIF20A in patients with early-stage cervical squamous cell carcinoma.
We examined the mRNA and protein levels of KIF20A in eight cervical cancer cell lines and eight paired cervical cancer samples, compared with normal cervical epithelial cells and adjacent normal cervical tissues, respectively. Immunohistochemistry was performed to detect the expression of KIF20A in paraffin-embedded specimens from 169 early-stage cervical squamous cell carcinoma patients. Statistical analyses were applied to analyze the association between KIF20A expression and clinical variables, as well with patient survival.
The mRNA and protein expression levels of KIF20A were significantly elevated in cervical cancer cell lines and lesions compared with normal cells and corresponding normal tissues (P < 0.05). Immunohistochemistry analysis in 169 cervical cancer cases revealed that increased KIF20A expression was strongly associated with human papillomavirus (HPV) infection (P = 0.008), clinical stage (P = 0.001), tumor recurrence (P = 0.016), vital status (P < 0.001), the property of the surgical margin (P = 0.032), the lymphovascular space involvement (P = 0.014), and pelvic lymph node metastasis (P = 0.001). The overall survival and disease-free survival of patients with high levels of KIF20A expression were significantly poorer than those with low KIF20A expression. KIF20A was an independent survival prognostic factor, as evidenced by univariate and multivariate analysis.
Our results illustrate that elevated KIF20A expression correlates with HPV infection, clinical stage, tumor recurrence, lymphovascular space involvement, pelvic lymph node metastasis, and poor outcome in early-stage cervical squamous cell carcinoma patients. KIF20A aberrant expression is a novel independent unfavorable prognostic factor and may present a potential therapeutic target for cervical cancer.</description><subject>Aberration</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - mortality</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - therapy</subject><subject>Cell division</subject><subject>Cell Line, Tumor</subject><subject>Cell survival</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Collaboration</subject><subject>Combined Modality Therapy</subject><subject>Comparative analysis</subject><subject>Development and progression</subject><subject>Disease Progression</subject><subject>Epithelial cells</subject><subject>Ethics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Hospitals</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Infections</subject><subject>Kaplan-Meier Estimate</subject><subject>Kinesin</subject><subject>Kinesin - genetics</subject><subject>Laboratories</subject><subject>Lesions</subject><subject>Lymph</subject><subject>Lymph nodes</subject><subject>Lymphatic Metastasis</subject><subject>Lymphatic system</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Melanoma</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>mRNA</subject><subject>Multivariate analysis</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Staging</subject><subject>Oncology</subject><subject>Pancreatic cancer</subject><subject>Papillomaviridae</subject><subject>Paraffin</subject><subject>Patients</subject><subject>Prognosis</subject><subject>Proportional Hazards Models</subject><subject>Proteins</subject><subject>Radiation therapy</subject><subject>Research and Analysis Methods</subject><subject>Squamous cell carcinoma</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Studies</subject><subject>Surgery</subject><subject>Survival</subject><subject>Tissues</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - mortality</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Uterine Cervical Neoplasms - therapy</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYqPwBggsISG4aPG_JPYNUlV1rGJSJzq4tVznNHWVxJ2dlO0NeGxc2k4t2sXkC1vHv_P5-LNPkrwleEBYTr6sXOcbXQ3WroEBJlnOuXyWnBPJaD-jmD0_Wp8lr0JYYZwykWUvkzOaS06kpOfJn0tbLtH4bu0hBOsa5Bbo--SC4iGaBDQMwRmrWyjQb9su0bVzHk034HVVoVnnN3ajK6SbAt10ddy69q48CNkGjbWv7vuzVpeARhBpE-nZbadr14UYiSIj7Y1tXK1fJy8WugrwZj_3kp8X45vRZf9q-m0yGl71TU7Ttk8ZlpoYBiljBeepKDCDuTGFpEDTFDMGGjM9F8CYwIUpmADCCOQizyETkvWS9zvddeWC2psYFBFcEMy2Cr1ksiMKp1dq7W2t_b1y2qp_AedLpX1rTQVqnkopuDGwEJRzonVWUDIXPAUp5oUQUevr_rRuXkNhoGmjdyeipzuNXarSbVRKUioojQKf9gLe3XYQWlXbYKJzuoFoYqw7lVxmXOCnoDTLMiJJRD_8hz5uxJ4qdbyrbRYulmi2omrIc5blJONppAaPUHEUUFsTP-fCxvhJwueThMi0cNeWugtBTWY_ns5Of52yH4_YJeiqXQZXdW38jeEU5DvQeBeCh8XDexCstr11cENte0vteyumvTt-y4ekQzOxv8LHHcU</recordid><startdate>20161212</startdate><enddate>20161212</enddate><creator>Zhang, Weijing</creator><creator>He, Weiling</creator><creator>Shi, Yongjie</creator><creator>Gu, Haifeng</creator><creator>Li, Min</creator><creator>Liu, Zhimin</creator><creator>Feng, Yanling</creator><creator>Zheng, Nianzhen</creator><creator>Xie, Chuanmiao</creator><creator>Zhang, Yanna</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20161212</creationdate><title>High Expression of KIF20A Is Associated with Poor Overall Survival and Tumor Progression in Early-Stage Cervical Squamous Cell Carcinoma</title><author>Zhang, Weijing ; He, Weiling ; Shi, Yongjie ; Gu, Haifeng ; Li, Min ; Liu, Zhimin ; Feng, Yanling ; Zheng, Nianzhen ; Xie, Chuanmiao ; Zhang, Yanna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-2309a1c3e533d4458d03ebccd92e255033ea03ab8e3380dcd38e131e7877e6893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aberration</topic><topic>Adult</topic><topic>Aged</topic><topic>Antigens</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - mortality</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - therapy</topic><topic>Cell division</topic><topic>Cell Line, Tumor</topic><topic>Cell survival</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Collaboration</topic><topic>Combined Modality Therapy</topic><topic>Comparative analysis</topic><topic>Development and progression</topic><topic>Disease Progression</topic><topic>Epithelial cells</topic><topic>Ethics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Hospitals</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Infections</topic><topic>Kaplan-Meier Estimate</topic><topic>Kinesin</topic><topic>Kinesin - genetics</topic><topic>Laboratories</topic><topic>Lesions</topic><topic>Lymph</topic><topic>Lymph nodes</topic><topic>Lymphatic Metastasis</topic><topic>Lymphatic system</topic><topic>Medical prognosis</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Melanoma</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>mRNA</topic><topic>Multivariate analysis</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Staging</topic><topic>Oncology</topic><topic>Pancreatic cancer</topic><topic>Papillomaviridae</topic><topic>Paraffin</topic><topic>Patients</topic><topic>Prognosis</topic><topic>Proportional Hazards Models</topic><topic>Proteins</topic><topic>Radiation therapy</topic><topic>Research and Analysis Methods</topic><topic>Squamous cell carcinoma</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Studies</topic><topic>Surgery</topic><topic>Survival</topic><topic>Tissues</topic><topic>Tumor cell lines</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Uterine Cervical Neoplasms - mortality</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Uterine Cervical Neoplasms - therapy</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Weijing</creatorcontrib><creatorcontrib>He, Weiling</creatorcontrib><creatorcontrib>Shi, Yongjie</creatorcontrib><creatorcontrib>Gu, Haifeng</creatorcontrib><creatorcontrib>Li, Min</creatorcontrib><creatorcontrib>Liu, Zhimin</creatorcontrib><creatorcontrib>Feng, Yanling</creatorcontrib><creatorcontrib>Zheng, Nianzhen</creatorcontrib><creatorcontrib>Xie, Chuanmiao</creatorcontrib><creatorcontrib>Zhang, Yanna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Weijing</au><au>He, Weiling</au><au>Shi, Yongjie</au><au>Gu, Haifeng</au><au>Li, Min</au><au>Liu, Zhimin</au><au>Feng, Yanling</au><au>Zheng, Nianzhen</au><au>Xie, Chuanmiao</au><au>Zhang, Yanna</au><au>Coleman, William B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High Expression of KIF20A Is Associated with Poor Overall Survival and Tumor Progression in Early-Stage Cervical Squamous Cell Carcinoma</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-12-12</date><risdate>2016</risdate><volume>11</volume><issue>12</issue><spage>e0167449</spage><epage>e0167449</epage><pages>e0167449-e0167449</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The kinesin family member 20a (KIF20A) protein has been implicated in the development and progression of many human cancers; however, its precise function and role in cervical cancer remain largely unclear. This study aimed to investigate the expression profile and prognostic value of KIF20A in patients with early-stage cervical squamous cell carcinoma.
We examined the mRNA and protein levels of KIF20A in eight cervical cancer cell lines and eight paired cervical cancer samples, compared with normal cervical epithelial cells and adjacent normal cervical tissues, respectively. Immunohistochemistry was performed to detect the expression of KIF20A in paraffin-embedded specimens from 169 early-stage cervical squamous cell carcinoma patients. Statistical analyses were applied to analyze the association between KIF20A expression and clinical variables, as well with patient survival.
The mRNA and protein expression levels of KIF20A were significantly elevated in cervical cancer cell lines and lesions compared with normal cells and corresponding normal tissues (P < 0.05). Immunohistochemistry analysis in 169 cervical cancer cases revealed that increased KIF20A expression was strongly associated with human papillomavirus (HPV) infection (P = 0.008), clinical stage (P = 0.001), tumor recurrence (P = 0.016), vital status (P < 0.001), the property of the surgical margin (P = 0.032), the lymphovascular space involvement (P = 0.014), and pelvic lymph node metastasis (P = 0.001). The overall survival and disease-free survival of patients with high levels of KIF20A expression were significantly poorer than those with low KIF20A expression. KIF20A was an independent survival prognostic factor, as evidenced by univariate and multivariate analysis.
Our results illustrate that elevated KIF20A expression correlates with HPV infection, clinical stage, tumor recurrence, lymphovascular space involvement, pelvic lymph node metastasis, and poor outcome in early-stage cervical squamous cell carcinoma patients. KIF20A aberrant expression is a novel independent unfavorable prognostic factor and may present a potential therapeutic target for cervical cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27941992</pmid><doi>10.1371/journal.pone.0167449</doi><tpages>e0167449</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-12, Vol.11 (12), p.e0167449-e0167449 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1848103550 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aberration Adult Aged Antigens Biology and Life Sciences Biotechnology Breast cancer Cancer Cancer therapies Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - mortality Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - therapy Cell division Cell Line, Tumor Cell survival Cervical cancer Cervix Collaboration Combined Modality Therapy Comparative analysis Development and progression Disease Progression Epithelial cells Ethics Female Gene Expression Gene Expression Regulation, Neoplastic Genetic aspects Hospitals Human papillomavirus Humans Immunohistochemistry Infections Kaplan-Meier Estimate Kinesin Kinesin - genetics Laboratories Lesions Lymph Lymph nodes Lymphatic Metastasis Lymphatic system Medical prognosis Medicine Medicine and Health Sciences Melanoma Metastases Metastasis Middle Aged mRNA Multivariate analysis Neoplasm Grading Neoplasm Staging Oncology Pancreatic cancer Papillomaviridae Paraffin Patients Prognosis Proportional Hazards Models Proteins Radiation therapy Research and Analysis Methods Squamous cell carcinoma Statistical analysis Statistical methods Studies Surgery Survival Tissues Tumor cell lines Tumors Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - mortality Uterine Cervical Neoplasms - pathology Uterine Cervical Neoplasms - therapy Womens health |
| title | High Expression of KIF20A Is Associated with Poor Overall Survival and Tumor Progression in Early-Stage Cervical Squamous Cell Carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-01T07%3A51%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High%20Expression%20of%20KIF20A%20Is%20Associated%20with%20Poor%20Overall%20Survival%20and%20Tumor%20Progression%20in%20Early-Stage%20Cervical%20Squamous%20Cell%20Carcinoma&rft.jtitle=PloS%20one&rft.au=Zhang,%20Weijing&rft.date=2016-12-12&rft.volume=11&rft.issue=12&rft.spage=e0167449&rft.epage=e0167449&rft.pages=e0167449-e0167449&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0167449&rft_dat=%3Cgale_plos_%3EA473671645%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1848103550&rft_id=info:pmid/27941992&rft_galeid=A473671645&rft_doaj_id=oai_doaj_org_article_b59984ccef82441aa6d21b845e98bd88&rfr_iscdi=true |