Microbial Diversity of Source and Point-of-Use Water in Rural Haiti - A Pyrosequencing-Based Metagenomic Survey
Haiti endures the poorest water and sanitation infrastructure in the Western Hemisphere, where waterborne diseases cause significant morbidity and mortality. Most of these diseases are reported to be caused by waterborne pathogens. In this study, we examined the overall bacterial diversity of select...
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| Veröffentlicht in: | PloS one 2016-12, Vol.11 (12), p.e0167353-e0167353 |
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| creator | Mukherjee, Nabanita Bartelli, Debra Patra, Cyril Chauhan, Bhavin V Dowd, Scot E Banerjee, Pratik |
| description | Haiti endures the poorest water and sanitation infrastructure in the Western Hemisphere, where waterborne diseases cause significant morbidity and mortality. Most of these diseases are reported to be caused by waterborne pathogens. In this study, we examined the overall bacterial diversity of selected source and point-of-use water from rural areas in Central Plateau, Haiti using pyrosequencing of 16s rRNA genes. Taxonomic composition of water samples revealed an abundance of Firmicutes phyla, followed by Proteobacteria and Bacteroidetes. A total of 38 bacterial families and 60 genera were identified. The presence of several Klebsiella spp. (tentatively, K. pneumoniae, K. variicola and other Klebsiella spp.) was detected in most water samples. Several other human pathogens such as Aeromonas, Bacillus, Clostridium, and Yersinia constituted significantly higher proportion of bacterial communities in the point-of-use water samples compared to source water. Bacterial genera traditionally associated with biofilm formation, such as Chryseobacterium, Fusobacterium, Prevotella, Pseudomonas were found in the point-of-use waters obtained from water filters or domestic water storage containers. Although the pyrosequencing method utilized in this study did not reveal the viability status of these pathogens, the abundance of genetic footprints of the pathogens in water samples indicate the probable risk of bacterial transmission to humans. Therefore, the importance of appropriate handling, purification, and treatment of the source water needed to be clearly communicated to the communities in rural Haiti to ensure the water is safe for their daily use and intake. |
| doi_str_mv | 10.1371/journal.pone.0167353 |
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Most of these diseases are reported to be caused by waterborne pathogens. In this study, we examined the overall bacterial diversity of selected source and point-of-use water from rural areas in Central Plateau, Haiti using pyrosequencing of 16s rRNA genes. Taxonomic composition of water samples revealed an abundance of Firmicutes phyla, followed by Proteobacteria and Bacteroidetes. A total of 38 bacterial families and 60 genera were identified. The presence of several Klebsiella spp. (tentatively, K. pneumoniae, K. variicola and other Klebsiella spp.) was detected in most water samples. Several other human pathogens such as Aeromonas, Bacillus, Clostridium, and Yersinia constituted significantly higher proportion of bacterial communities in the point-of-use water samples compared to source water. Bacterial genera traditionally associated with biofilm formation, such as Chryseobacterium, Fusobacterium, Prevotella, Pseudomonas were found in the point-of-use waters obtained from water filters or domestic water storage containers. Although the pyrosequencing method utilized in this study did not reveal the viability status of these pathogens, the abundance of genetic footprints of the pathogens in water samples indicate the probable risk of bacterial transmission to humans. Therefore, the importance of appropriate handling, purification, and treatment of the source water needed to be clearly communicated to the communities in rural Haiti to ensure the water is safe for their daily use and intake.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0167353</identifier><identifier>PMID: 27936055</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject><![CDATA[Abundance ; Aeromonas ; Analysis ; Bacillus ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteroidetes - classification ; Bacteroidetes - genetics ; Bacteroidetes - isolation & purification ; Biodiversity ; Biofilms ; Biology and Life Sciences ; Care and treatment ; Chryseobacterium ; Clostridium ; Containers ; Disease transmission ; Domestic water ; Drinking water ; Earth Sciences ; Environmental health ; Epidemiology ; Firmicutes ; Firmicutes - classification ; Firmicutes - genetics ; Firmicutes - isolation & purification ; Fusobacterium ; Genera ; Haiti ; Humans ; Identification ; Klebsiella ; Klebsiella pneumoniae ; Management ; Medicine and Health Sciences ; Metagenomics ; Microorganisms ; Morbidity ; Pathogens ; Population ; Prevotella ; Proteobacteria ; Proteobacteria - classification ; Proteobacteria - genetics ; Proteobacteria - isolation & purification ; Pseudomonas ; Public health ; Purification ; Risk assessment ; Risk factors ; RNA, Bacterial - genetics ; RNA, Bacterial - isolation & purification ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 16S - isolation & purification ; rRNA 16S ; Rural areas ; Sanitation ; Storage containers ; Studies ; Surveys ; Urinary tract diseases ; Urinary tract infections ; Urogenital system ; Viability ; Water analysis ; Water filters ; Water Microbiology ; Water purification ; Water quality ; Water resource management ; Water sampling ; Water storage ; Water treatment ; Waterborne infections ; Western Hemisphere ; Yersinia]]></subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0167353-e0167353</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Mukherjee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Most of these diseases are reported to be caused by waterborne pathogens. In this study, we examined the overall bacterial diversity of selected source and point-of-use water from rural areas in Central Plateau, Haiti using pyrosequencing of 16s rRNA genes. Taxonomic composition of water samples revealed an abundance of Firmicutes phyla, followed by Proteobacteria and Bacteroidetes. A total of 38 bacterial families and 60 genera were identified. The presence of several Klebsiella spp. (tentatively, K. pneumoniae, K. variicola and other Klebsiella spp.) was detected in most water samples. Several other human pathogens such as Aeromonas, Bacillus, Clostridium, and Yersinia constituted significantly higher proportion of bacterial communities in the point-of-use water samples compared to source water. Bacterial genera traditionally associated with biofilm formation, such as Chryseobacterium, Fusobacterium, Prevotella, Pseudomonas were found in the point-of-use waters obtained from water filters or domestic water storage containers. Although the pyrosequencing method utilized in this study did not reveal the viability status of these pathogens, the abundance of genetic footprints of the pathogens in water samples indicate the probable risk of bacterial transmission to humans. 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Most of these diseases are reported to be caused by waterborne pathogens. In this study, we examined the overall bacterial diversity of selected source and point-of-use water from rural areas in Central Plateau, Haiti using pyrosequencing of 16s rRNA genes. Taxonomic composition of water samples revealed an abundance of Firmicutes phyla, followed by Proteobacteria and Bacteroidetes. A total of 38 bacterial families and 60 genera were identified. The presence of several Klebsiella spp. (tentatively, K. pneumoniae, K. variicola and other Klebsiella spp.) was detected in most water samples. Several other human pathogens such as Aeromonas, Bacillus, Clostridium, and Yersinia constituted significantly higher proportion of bacterial communities in the point-of-use water samples compared to source water. Bacterial genera traditionally associated with biofilm formation, such as Chryseobacterium, Fusobacterium, Prevotella, Pseudomonas were found in the point-of-use waters obtained from water filters or domestic water storage containers. Although the pyrosequencing method utilized in this study did not reveal the viability status of these pathogens, the abundance of genetic footprints of the pathogens in water samples indicate the probable risk of bacterial transmission to humans. Therefore, the importance of appropriate handling, purification, and treatment of the source water needed to be clearly communicated to the communities in rural Haiti to ensure the water is safe for their daily use and intake.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27936055</pmid><doi>10.1371/journal.pone.0167353</doi><tpages>e0167353</tpages><orcidid>https://orcid.org/0000-0001-5553-5350</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-12, Vol.11 (12), p.e0167353-e0167353 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1847563207 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abundance Aeromonas Analysis Bacillus Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteroidetes - classification Bacteroidetes - genetics Bacteroidetes - isolation & purification Biodiversity Biofilms Biology and Life Sciences Care and treatment Chryseobacterium Clostridium Containers Disease transmission Domestic water Drinking water Earth Sciences Environmental health Epidemiology Firmicutes Firmicutes - classification Firmicutes - genetics Firmicutes - isolation & purification Fusobacterium Genera Haiti Humans Identification Klebsiella Klebsiella pneumoniae Management Medicine and Health Sciences Metagenomics Microorganisms Morbidity Pathogens Population Prevotella Proteobacteria Proteobacteria - classification Proteobacteria - genetics Proteobacteria - isolation & purification Pseudomonas Public health Purification Risk assessment Risk factors RNA, Bacterial - genetics RNA, Bacterial - isolation & purification RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 16S - isolation & purification rRNA 16S Rural areas Sanitation Storage containers Studies Surveys Urinary tract diseases Urinary tract infections Urogenital system Viability Water analysis Water filters Water Microbiology Water purification Water quality Water resource management Water sampling Water storage Water treatment Waterborne infections Western Hemisphere Yersinia |
| title | Microbial Diversity of Source and Point-of-Use Water in Rural Haiti - A Pyrosequencing-Based Metagenomic Survey |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T23%3A12%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microbial%20Diversity%20of%20Source%20and%20Point-of-Use%20Water%20in%20Rural%20Haiti%20-%20A%20Pyrosequencing-Based%20Metagenomic%20Survey&rft.jtitle=PloS%20one&rft.au=Mukherjee,%20Nabanita&rft.date=2016-12-09&rft.volume=11&rft.issue=12&rft.spage=e0167353&rft.epage=e0167353&rft.pages=e0167353-e0167353&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0167353&rft_dat=%3Cgale_plos_%3EA473427986%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1847563207&rft_id=info:pmid/27936055&rft_galeid=A473427986&rft_doaj_id=oai_doaj_org_article_79689e0641f8431aa7adbc80b4178306&rfr_iscdi=true |