High-Speed Mouse Backcrossing Through the Female Germ Line
Transferring mouse mutations into specific mouse strain backgrounds can be critical for appropriate analysis of phenotypic effects of targeted genomic alterations and quantitative trait loci. Speed congenic breeding strategies incorporating marker-assisted selection of progeny with the highest perce...
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| Veröffentlicht in: | PloS one 2016-12, Vol.11 (12), p.e0166822-e0166822 |
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| creator | Grove, Erin Eckardt, Sigrid McLaughlin, K John |
| description | Transferring mouse mutations into specific mouse strain backgrounds can be critical for appropriate analysis of phenotypic effects of targeted genomic alterations and quantitative trait loci. Speed congenic breeding strategies incorporating marker-assisted selection of progeny with the highest percentage target background as breeders for the next generation can produce congenic strains within approximately 5 generations. When mating selected donor males to target strain females, this may require more than 1 year, with each generation lasting 10 to 11 weeks including 3 weeks of gestation and 7 to 8 weeks until the males reach sexual maturity. Because ovulation can be induced in female mice as early as 3 weeks of age, superovulation-aided backcrossing of marker-selected females could accelerate the production of congenic animals by approximately 4 weeks per generation, reducing time and cost. Using this approach, we transferred a transgenic strain of undefined genetic background to >99% C57BL/6J within 10 months, with most generations lasting 7 weeks. This involved less than 60 mice in total, with 9 to 18 animals per generation. Our data demonstrate that high-speed backcrossing through the female germline is feasible and practical with small mouse numbers. |
| doi_str_mv | 10.1371/journal.pone.0166822 |
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Speed congenic breeding strategies incorporating marker-assisted selection of progeny with the highest percentage target background as breeders for the next generation can produce congenic strains within approximately 5 generations. When mating selected donor males to target strain females, this may require more than 1 year, with each generation lasting 10 to 11 weeks including 3 weeks of gestation and 7 to 8 weeks until the males reach sexual maturity. Because ovulation can be induced in female mice as early as 3 weeks of age, superovulation-aided backcrossing of marker-selected females could accelerate the production of congenic animals by approximately 4 weeks per generation, reducing time and cost. Using this approach, we transferred a transgenic strain of undefined genetic background to >99% C57BL/6J within 10 months, with most generations lasting 7 weeks. This involved less than 60 mice in total, with 9 to 18 animals per generation. Our data demonstrate that high-speed backcrossing through the female germline is feasible and practical with small mouse numbers.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0166822</identifier><identifier>PMID: 27926922</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Animal genetic engineering ; Animals ; Animals, Congenic - physiology ; Biology and Life Sciences ; Breeding ; Diabetes ; DNA methylation ; Embryos ; Female ; Females ; Gene expression ; Genetically modified mice ; Genetics ; Genomes ; Germ Cells - physiology ; Gestation ; High speed ; Inbreeding - methods ; Insulin ; Laboratory animals ; Male ; Males ; Marker-assisted selection ; Mating ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Mutation ; Ovulation ; Phenotype ; Progeny ; Quantitative trait loci ; Quantitative Trait Loci - physiology ; Reproduction - physiology ; Research and Analysis Methods ; Rodents ; Sexual maturity</subject><ispartof>PloS one, 2016-12, Vol.11 (12), p.e0166822-e0166822</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Grove et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Grove et al 2016 Grove et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-f5d4428bc5b6b2ccf0bf11f091d61e79fbf707b0954690cc92553a1c953eac93</citedby><cites>FETCH-LOGICAL-c725t-f5d4428bc5b6b2ccf0bf11f091d61e79fbf707b0954690cc92553a1c953eac93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142779/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142779/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23868,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27926922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hohenstein, Peter</contributor><creatorcontrib>Grove, Erin</creatorcontrib><creatorcontrib>Eckardt, Sigrid</creatorcontrib><creatorcontrib>McLaughlin, K John</creatorcontrib><title>High-Speed Mouse Backcrossing Through the Female Germ Line</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Transferring mouse mutations into specific mouse strain backgrounds can be critical for appropriate analysis of phenotypic effects of targeted genomic alterations and quantitative trait loci. Speed congenic breeding strategies incorporating marker-assisted selection of progeny with the highest percentage target background as breeders for the next generation can produce congenic strains within approximately 5 generations. When mating selected donor males to target strain females, this may require more than 1 year, with each generation lasting 10 to 11 weeks including 3 weeks of gestation and 7 to 8 weeks until the males reach sexual maturity. Because ovulation can be induced in female mice as early as 3 weeks of age, superovulation-aided backcrossing of marker-selected females could accelerate the production of congenic animals by approximately 4 weeks per generation, reducing time and cost. Using this approach, we transferred a transgenic strain of undefined genetic background to >99% C57BL/6J within 10 months, with most generations lasting 7 weeks. This involved less than 60 mice in total, with 9 to 18 animals per generation. Our data demonstrate that high-speed backcrossing through the female germline is feasible and practical with small mouse numbers.</description><subject>Age</subject><subject>Animal genetic engineering</subject><subject>Animals</subject><subject>Animals, Congenic - physiology</subject><subject>Biology and Life Sciences</subject><subject>Breeding</subject><subject>Diabetes</subject><subject>DNA methylation</subject><subject>Embryos</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Genetically modified mice</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Germ Cells - physiology</subject><subject>Gestation</subject><subject>High speed</subject><subject>Inbreeding - methods</subject><subject>Insulin</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Males</subject><subject>Marker-assisted selection</subject><subject>Mating</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mutation</subject><subject>Ovulation</subject><subject>Phenotype</subject><subject>Progeny</subject><subject>Quantitative trait loci</subject><subject>Quantitative Trait Loci - 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physiology</topic><topic>Biology and Life Sciences</topic><topic>Breeding</topic><topic>Diabetes</topic><topic>DNA methylation</topic><topic>Embryos</topic><topic>Female</topic><topic>Females</topic><topic>Gene expression</topic><topic>Genetically modified mice</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Germ Cells - physiology</topic><topic>Gestation</topic><topic>High speed</topic><topic>Inbreeding - methods</topic><topic>Insulin</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Males</topic><topic>Marker-assisted selection</topic><topic>Mating</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mutation</topic><topic>Ovulation</topic><topic>Phenotype</topic><topic>Progeny</topic><topic>Quantitative trait loci</topic><topic>Quantitative Trait Loci - physiology</topic><topic>Reproduction - physiology</topic><topic>Research and Analysis Methods</topic><topic>Rodents</topic><topic>Sexual maturity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grove, Erin</creatorcontrib><creatorcontrib>Eckardt, Sigrid</creatorcontrib><creatorcontrib>McLaughlin, K John</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Science (Gale in Context)</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grove, Erin</au><au>Eckardt, Sigrid</au><au>McLaughlin, K John</au><au>Hohenstein, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Speed Mouse Backcrossing Through the Female Germ Line</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-12-07</date><risdate>2016</risdate><volume>11</volume><issue>12</issue><spage>e0166822</spage><epage>e0166822</epage><pages>e0166822-e0166822</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Transferring mouse mutations into specific mouse strain backgrounds can be critical for appropriate analysis of phenotypic effects of targeted genomic alterations and quantitative trait loci. Speed congenic breeding strategies incorporating marker-assisted selection of progeny with the highest percentage target background as breeders for the next generation can produce congenic strains within approximately 5 generations. When mating selected donor males to target strain females, this may require more than 1 year, with each generation lasting 10 to 11 weeks including 3 weeks of gestation and 7 to 8 weeks until the males reach sexual maturity. Because ovulation can be induced in female mice as early as 3 weeks of age, superovulation-aided backcrossing of marker-selected females could accelerate the production of congenic animals by approximately 4 weeks per generation, reducing time and cost. Using this approach, we transferred a transgenic strain of undefined genetic background to >99% C57BL/6J within 10 months, with most generations lasting 7 weeks. This involved less than 60 mice in total, with 9 to 18 animals per generation. Our data demonstrate that high-speed backcrossing through the female germline is feasible and practical with small mouse numbers.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27926922</pmid><doi>10.1371/journal.pone.0166822</doi><tpages>e0166822</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Age Animal genetic engineering Animals Animals, Congenic - physiology Biology and Life Sciences Breeding Diabetes DNA methylation Embryos Female Females Gene expression Genetically modified mice Genetics Genomes Germ Cells - physiology Gestation High speed Inbreeding - methods Insulin Laboratory animals Male Males Marker-assisted selection Mating Medicine and Health Sciences Mice Mice, Inbred C57BL Mutation Ovulation Phenotype Progeny Quantitative trait loci Quantitative Trait Loci - physiology Reproduction - physiology Research and Analysis Methods Rodents Sexual maturity |
| title | High-Speed Mouse Backcrossing Through the Female Germ Line |
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