Mycobacterium ulcerans Mouse Model Refinement for Pre-Clinical Profiling of Vaccine Candidates
Buruli Ulcer is a neglected tropical disease leading to extensive disabilities and morbidity in West Africa. In this paper we sought to characterize various strains of Mycobacterium ulcerans (M.ulcerans) with different origins and laboratory passage records while refining a mouse model for Buruli ul...
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| description | Buruli Ulcer is a neglected tropical disease leading to extensive disabilities and morbidity in West Africa. In this paper we sought to characterize various strains of Mycobacterium ulcerans (M.ulcerans) with different origins and laboratory passage records while refining a mouse model for Buruli ulcer. We described, compared and followed the kinetics of the histo-pathological outcome of infection of a collection of strains at various anatomical sites of infection in order to find a suitable model for further immunization studies. Moreover we compared the outcome of infection in C57Bl/6 and Balbc/J mice. Specifically we described thoroughly one M. ulcerans strain characterized by slow growth rate and limited tissue necrosis, which presents close ressemblance with the infection kinetics in humans. This strain caused macrophages as well as T and B cells infiltration, correlating with mycobacterial proliferation at the site of infection as well as in the draining lymph nodes, making it a suitable strain to screen vaccine candidates efficacy. |
| doi_str_mv | 10.1371/journal.pone.0167059 |
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In this paper we sought to characterize various strains of Mycobacterium ulcerans (M.ulcerans) with different origins and laboratory passage records while refining a mouse model for Buruli ulcer. We described, compared and followed the kinetics of the histo-pathological outcome of infection of a collection of strains at various anatomical sites of infection in order to find a suitable model for further immunization studies. Moreover we compared the outcome of infection in C57Bl/6 and Balbc/J mice. Specifically we described thoroughly one M. ulcerans strain characterized by slow growth rate and limited tissue necrosis, which presents close ressemblance with the infection kinetics in humans. This strain caused macrophages as well as T and B cells infiltration, correlating with mycobacterial proliferation at the site of infection as well as in the draining lymph nodes, making it a suitable strain to screen vaccine candidates efficacy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0167059</identifier><identifier>PMID: 27893778</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; B cells ; Bacterial Vaccines - administration & dosage ; Bacterial Vaccines - immunology ; Biology and Life Sciences ; Buruli ulcer ; Buruli Ulcer - immunology ; Buruli Ulcer - pathology ; Buruli Ulcer - prevention & control ; Cell proliferation ; Disabilities ; Disease ; Disease Models, Animal ; Diseases ; Drug Evaluation, Preclinical ; Foot - microbiology ; Foot - pathology ; Genomes ; Growth rate ; Health aspects ; House mouse ; Immune system ; Immunization ; Infections ; Infiltration ; Inflammation - immunology ; Inflammation - microbiology ; Inflammation - prevention & control ; Kinetics ; Lymph nodes ; Lymphocytes B ; Macrophages ; Macrophages - drug effects ; Macrophages - immunology ; Macrophages - microbiology ; Mammals ; Medicine and Health Sciences ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Morbidity ; Mycobacterium ; Mycobacterium marinum ; Mycobacterium ulcerans ; Mycobacterium ulcerans - drug effects ; Mycobacterium ulcerans - immunology ; Public health ; Research and Analysis Methods ; Tuberculosis ; Ulcers ; Vaccines</subject><ispartof>PloS one, 2016-11, Vol.11 (11), p.e0167059-e0167059</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Bénard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Bénard et al 2016 Bénard et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-7de5d0acceb785241ff6dd0e9536cc922cf1cf63e884b6efc9309fb9920325d53</citedby><cites>FETCH-LOGICAL-c725t-7de5d0acceb785241ff6dd0e9536cc922cf1cf63e884b6efc9309fb9920325d53</cites><orcidid>0000-0003-2099-149X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125663/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125663/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27893778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Cardona, Pere-Joan</contributor><creatorcontrib>Bénard, Angèle</creatorcontrib><creatorcontrib>Sala, Claudia</creatorcontrib><creatorcontrib>Pluschke, Gerd</creatorcontrib><title>Mycobacterium ulcerans Mouse Model Refinement for Pre-Clinical Profiling of Vaccine Candidates</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Buruli Ulcer is a neglected tropical disease leading to extensive disabilities and morbidity in West Africa. In this paper we sought to characterize various strains of Mycobacterium ulcerans (M.ulcerans) with different origins and laboratory passage records while refining a mouse model for Buruli ulcer. We described, compared and followed the kinetics of the histo-pathological outcome of infection of a collection of strains at various anatomical sites of infection in order to find a suitable model for further immunization studies. Moreover we compared the outcome of infection in C57Bl/6 and Balbc/J mice. Specifically we described thoroughly one M. ulcerans strain characterized by slow growth rate and limited tissue necrosis, which presents close ressemblance with the infection kinetics in humans. This strain caused macrophages as well as T and B cells infiltration, correlating with mycobacterial proliferation at the site of infection as well as in the draining lymph nodes, making it a suitable strain to screen vaccine candidates efficacy.</description><subject>Analysis</subject><subject>Animals</subject><subject>B cells</subject><subject>Bacterial Vaccines - administration & dosage</subject><subject>Bacterial Vaccines - immunology</subject><subject>Biology and Life Sciences</subject><subject>Buruli ulcer</subject><subject>Buruli Ulcer - immunology</subject><subject>Buruli Ulcer - pathology</subject><subject>Buruli Ulcer - prevention & control</subject><subject>Cell proliferation</subject><subject>Disabilities</subject><subject>Disease</subject><subject>Disease Models, Animal</subject><subject>Diseases</subject><subject>Drug Evaluation, Preclinical</subject><subject>Foot - microbiology</subject><subject>Foot - pathology</subject><subject>Genomes</subject><subject>Growth rate</subject><subject>Health aspects</subject><subject>House mouse</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Infections</subject><subject>Infiltration</subject><subject>Inflammation - 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administration & dosage</topic><topic>Bacterial Vaccines - immunology</topic><topic>Biology and Life Sciences</topic><topic>Buruli ulcer</topic><topic>Buruli Ulcer - immunology</topic><topic>Buruli Ulcer - pathology</topic><topic>Buruli Ulcer - prevention & control</topic><topic>Cell proliferation</topic><topic>Disabilities</topic><topic>Disease</topic><topic>Disease Models, Animal</topic><topic>Diseases</topic><topic>Drug Evaluation, Preclinical</topic><topic>Foot - microbiology</topic><topic>Foot - pathology</topic><topic>Genomes</topic><topic>Growth rate</topic><topic>Health aspects</topic><topic>House mouse</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Infections</topic><topic>Infiltration</topic><topic>Inflammation - immunology</topic><topic>Inflammation - microbiology</topic><topic>Inflammation - prevention & control</topic><topic>Kinetics</topic><topic>Lymph nodes</topic><topic>Lymphocytes B</topic><topic>Macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bénard, Angèle</au><au>Sala, Claudia</au><au>Pluschke, Gerd</au><au>Cardona, Pere-Joan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycobacterium ulcerans Mouse Model Refinement for Pre-Clinical Profiling of Vaccine Candidates</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-11-28</date><risdate>2016</risdate><volume>11</volume><issue>11</issue><spage>e0167059</spage><epage>e0167059</epage><pages>e0167059-e0167059</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Buruli Ulcer is a neglected tropical disease leading to extensive disabilities and morbidity in West Africa. In this paper we sought to characterize various strains of Mycobacterium ulcerans (M.ulcerans) with different origins and laboratory passage records while refining a mouse model for Buruli ulcer. We described, compared and followed the kinetics of the histo-pathological outcome of infection of a collection of strains at various anatomical sites of infection in order to find a suitable model for further immunization studies. Moreover we compared the outcome of infection in C57Bl/6 and Balbc/J mice. Specifically we described thoroughly one M. ulcerans strain characterized by slow growth rate and limited tissue necrosis, which presents close ressemblance with the infection kinetics in humans. This strain caused macrophages as well as T and B cells infiltration, correlating with mycobacterial proliferation at the site of infection as well as in the draining lymph nodes, making it a suitable strain to screen vaccine candidates efficacy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27893778</pmid><doi>10.1371/journal.pone.0167059</doi><tpages>e0167059</tpages><orcidid>https://orcid.org/0000-0003-2099-149X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Animals B cells Bacterial Vaccines - administration & dosage Bacterial Vaccines - immunology Biology and Life Sciences Buruli ulcer Buruli Ulcer - immunology Buruli Ulcer - pathology Buruli Ulcer - prevention & control Cell proliferation Disabilities Disease Disease Models, Animal Diseases Drug Evaluation, Preclinical Foot - microbiology Foot - pathology Genomes Growth rate Health aspects House mouse Immune system Immunization Infections Infiltration Inflammation - immunology Inflammation - microbiology Inflammation - prevention & control Kinetics Lymph nodes Lymphocytes B Macrophages Macrophages - drug effects Macrophages - immunology Macrophages - microbiology Mammals Medicine and Health Sciences Mice Mice, Inbred BALB C Mice, Inbred C57BL Morbidity Mycobacterium Mycobacterium marinum Mycobacterium ulcerans Mycobacterium ulcerans - drug effects Mycobacterium ulcerans - immunology Public health Research and Analysis Methods Tuberculosis Ulcers Vaccines |
| title | Mycobacterium ulcerans Mouse Model Refinement for Pre-Clinical Profiling of Vaccine Candidates |
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