Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish
Hurdles in the treatment of retinal degeneration include managing the functional rewiring of surviving photoreceptors and integration of any newly added cells into the remaining second-order retinal neurons. Zebrafish are the premier genetic model for such questions, and we present two new transgeni...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-11, Vol.11 (11), p.e0166932-e0166932 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0166932 |
|---|---|
| container_issue | 11 |
| container_start_page | e0166932 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Hagerman, Gordon F Noel, Nicole C L Cao, Sylvia Y DuVal, Michèle G Oel, A Phillip Allison, W Ted |
| description | Hurdles in the treatment of retinal degeneration include managing the functional rewiring of surviving photoreceptors and integration of any newly added cells into the remaining second-order retinal neurons. Zebrafish are the premier genetic model for such questions, and we present two new transgenic lines allowing us to contrast vision loss and recovery following conditional ablation of specific cone types: UV or blue cones. The ablation of each cone type proved to be thorough (killing 80% of cells in each intended cone class), specific, and cell-autonomous. We assessed the loss and recovery of vision in larvae via the optomotor behavioural response (OMR). This visually mediated behaviour decreased to about 5% or 20% of control levels following ablation of UV or blue cones, respectively (P |
| doi_str_mv | 10.1371/journal.pone.0166932 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1844332559</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A471946473</galeid><doaj_id>oai_doaj_org_article_368d28fe86474c08a9f33cd824dbdc0f</doaj_id><sourcerecordid>A471946473</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-14228f21d4f263646ae32208ea205a7542b6b694e3bde5f30eafa97ee0f3e61c3</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYqPwDxBEQkJw0eKv2MkNUqkYVJo0UWAX3FiOc9y6cuMSJ4P9e5w1mxq0i8kXtuznvOfLJ0leYjTDVOAPW981tXKzva9hhjDnBSWPklMctykniD4-Op8kz0LYIpTRnPOnyQkReUGFKE6Tbyu1t1W6Au2voLlOvUkvbeiUS8-6WrfW1-k8BK-taqFK_9h2k35yHaSL6DWdl07dILZOf0HZKGPD5nnyxCgX4MWwT5KfZ59_LL5Ozy--LBfz86kWJGunmBGSG4IrZginnHEFlBCUgyIoUyJjpOQlLxjQsoLMUATKqEIAIEOBY00nyeuD7t75IIdqBIlzxiglWVZEYnkgKq-2ct_YnWqupVdW3lz4Zi1V01rtQFKeVzEcyDkTTKNcFYZSXeWEVWWlo8tJ8nHw1pU7qDTUbaPcSHT8UtuNXPsrmWGS8YxGgXeDQON_dxBaubNBg3OqBt_1cWdIxLZg8QCUMY5EzDKib_5D7y_EQK1VzNXWxscQdS8q50zggsWs-whn91BxVbCzOvbb2Hg_Mng_MohMC3_btepCkMvvq4ezF5dj9u0RuwHl2k3wruu_WhiD7ADqxofQgLnrB0ayH5Lbash-SOQwJNHs1XEv74xup4L-A_QMCss</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1844332559</pqid></control><display><type>article</type><title>Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Hagerman, Gordon F ; Noel, Nicole C L ; Cao, Sylvia Y ; DuVal, Michèle G ; Oel, A Phillip ; Allison, W Ted</creator><creatorcontrib>Hagerman, Gordon F ; Noel, Nicole C L ; Cao, Sylvia Y ; DuVal, Michèle G ; Oel, A Phillip ; Allison, W Ted</creatorcontrib><description>Hurdles in the treatment of retinal degeneration include managing the functional rewiring of surviving photoreceptors and integration of any newly added cells into the remaining second-order retinal neurons. Zebrafish are the premier genetic model for such questions, and we present two new transgenic lines allowing us to contrast vision loss and recovery following conditional ablation of specific cone types: UV or blue cones. The ablation of each cone type proved to be thorough (killing 80% of cells in each intended cone class), specific, and cell-autonomous. We assessed the loss and recovery of vision in larvae via the optomotor behavioural response (OMR). This visually mediated behaviour decreased to about 5% or 20% of control levels following ablation of UV or blue cones, respectively (P<0.05). We further assessed ocular photoreception by measuring the effects of UV light on body pigmentation, and observed that photoreceptor deficits and recovery occurred (p<0.01) with a timeline coincident to the OMR results. This corroborated and extended previous conclusions that UV cones are required photoreceptors for modulating body pigmentation, addressing assumptions that were unavoidable in previous experiments. Functional vision recovery following UV cone ablation was robust, as measured by both assays, returning to control levels within four days. In contrast, robust functional recovery following blue cone ablation was unexpectedly rapid, returning to normal levels within 24 hours after ablation. Ablation of cones led to increased proliferation in the retina, though the rapid recovery of vision following blue cone ablation was demonstrated to not be mediated by blue cone regeneration. Thus rapid visual recovery occurs following ablation of some, but not all, cone subtypes, suggesting an opportunity to contrast and dissect the sources and mechanisms of outer retinal recovery during cone photoreceptor death and regeneration.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0166932</identifier><identifier>PMID: 27893779</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Ablation (Surgery) ; Ablation Techniques ; Animals ; Animals, Genetically Modified ; Apoptosis ; Biology and Life Sciences ; Cell Death ; Cell Proliferation ; Cones ; Danio rerio ; Degeneration ; Genetics ; Larva ; Larvae ; Medicine and Health Sciences ; Melanins - metabolism ; Metronidazole - pharmacology ; Nitroreductases - genetics ; Nitroreductases - metabolism ; Oncorhynchus mykiss ; Photoreception ; Photoreceptors ; Physical Sciences ; Physiological aspects ; Pigmentation ; Recovery ; Recovery (Medical) ; Recovery of function ; Regeneration ; Research and Analysis Methods ; Retina ; Retina - cytology ; Retinal Cone Photoreceptor Cells - drug effects ; Retinal Cone Photoreceptor Cells - physiology ; Retinal Degeneration ; Rewiring ; Social Sciences ; Stem cells ; Thyroid gland ; Trout ; Ultraviolet radiation ; Ultraviolet Rays ; Vision ; Vision, Ocular - physiology ; Visual perception ; Zebrafish ; Zebrafish - genetics</subject><ispartof>PloS one, 2016-11, Vol.11 (11), p.e0166932-e0166932</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Hagerman et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Hagerman et al 2016 Hagerman et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-14228f21d4f263646ae32208ea205a7542b6b694e3bde5f30eafa97ee0f3e61c3</citedby><cites>FETCH-LOGICAL-c725t-14228f21d4f263646ae32208ea205a7542b6b694e3bde5f30eafa97ee0f3e61c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125653/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125653/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27893779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hagerman, Gordon F</creatorcontrib><creatorcontrib>Noel, Nicole C L</creatorcontrib><creatorcontrib>Cao, Sylvia Y</creatorcontrib><creatorcontrib>DuVal, Michèle G</creatorcontrib><creatorcontrib>Oel, A Phillip</creatorcontrib><creatorcontrib>Allison, W Ted</creatorcontrib><title>Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hurdles in the treatment of retinal degeneration include managing the functional rewiring of surviving photoreceptors and integration of any newly added cells into the remaining second-order retinal neurons. Zebrafish are the premier genetic model for such questions, and we present two new transgenic lines allowing us to contrast vision loss and recovery following conditional ablation of specific cone types: UV or blue cones. The ablation of each cone type proved to be thorough (killing 80% of cells in each intended cone class), specific, and cell-autonomous. We assessed the loss and recovery of vision in larvae via the optomotor behavioural response (OMR). This visually mediated behaviour decreased to about 5% or 20% of control levels following ablation of UV or blue cones, respectively (P<0.05). We further assessed ocular photoreception by measuring the effects of UV light on body pigmentation, and observed that photoreceptor deficits and recovery occurred (p<0.01) with a timeline coincident to the OMR results. This corroborated and extended previous conclusions that UV cones are required photoreceptors for modulating body pigmentation, addressing assumptions that were unavoidable in previous experiments. Functional vision recovery following UV cone ablation was robust, as measured by both assays, returning to control levels within four days. In contrast, robust functional recovery following blue cone ablation was unexpectedly rapid, returning to normal levels within 24 hours after ablation. Ablation of cones led to increased proliferation in the retina, though the rapid recovery of vision following blue cone ablation was demonstrated to not be mediated by blue cone regeneration. Thus rapid visual recovery occurs following ablation of some, but not all, cone subtypes, suggesting an opportunity to contrast and dissect the sources and mechanisms of outer retinal recovery during cone photoreceptor death and regeneration.</description><subject>Ablation (Surgery)</subject><subject>Ablation Techniques</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Apoptosis</subject><subject>Biology and Life Sciences</subject><subject>Cell Death</subject><subject>Cell Proliferation</subject><subject>Cones</subject><subject>Danio rerio</subject><subject>Degeneration</subject><subject>Genetics</subject><subject>Larva</subject><subject>Larvae</subject><subject>Medicine and Health Sciences</subject><subject>Melanins - metabolism</subject><subject>Metronidazole - pharmacology</subject><subject>Nitroreductases - genetics</subject><subject>Nitroreductases - metabolism</subject><subject>Oncorhynchus mykiss</subject><subject>Photoreception</subject><subject>Photoreceptors</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Pigmentation</subject><subject>Recovery</subject><subject>Recovery (Medical)</subject><subject>Recovery of function</subject><subject>Regeneration</subject><subject>Research and Analysis Methods</subject><subject>Retina</subject><subject>Retina - cytology</subject><subject>Retinal Cone Photoreceptor Cells - drug effects</subject><subject>Retinal Cone Photoreceptor Cells - physiology</subject><subject>Retinal Degeneration</subject><subject>Rewiring</subject><subject>Social Sciences</subject><subject>Stem cells</subject><subject>Thyroid gland</subject><subject>Trout</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><subject>Vision</subject><subject>Vision, Ocular - physiology</subject><subject>Visual perception</subject><subject>Zebrafish</subject><subject>Zebrafish - genetics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYqPwDxBEQkJw0eKv2MkNUqkYVJo0UWAX3FiOc9y6cuMSJ4P9e5w1mxq0i8kXtuznvOfLJ0leYjTDVOAPW981tXKzva9hhjDnBSWPklMctykniD4-Op8kz0LYIpTRnPOnyQkReUGFKE6Tbyu1t1W6Au2voLlOvUkvbeiUS8-6WrfW1-k8BK-taqFK_9h2k35yHaSL6DWdl07dILZOf0HZKGPD5nnyxCgX4MWwT5KfZ59_LL5Ozy--LBfz86kWJGunmBGSG4IrZginnHEFlBCUgyIoUyJjpOQlLxjQsoLMUATKqEIAIEOBY00nyeuD7t75IIdqBIlzxiglWVZEYnkgKq-2ct_YnWqupVdW3lz4Zi1V01rtQFKeVzEcyDkTTKNcFYZSXeWEVWWlo8tJ8nHw1pU7qDTUbaPcSHT8UtuNXPsrmWGS8YxGgXeDQON_dxBaubNBg3OqBt_1cWdIxLZg8QCUMY5EzDKib_5D7y_EQK1VzNXWxscQdS8q50zggsWs-whn91BxVbCzOvbb2Hg_Mng_MohMC3_btepCkMvvq4ezF5dj9u0RuwHl2k3wruu_WhiD7ADqxofQgLnrB0ayH5Lbash-SOQwJNHs1XEv74xup4L-A_QMCss</recordid><startdate>20161128</startdate><enddate>20161128</enddate><creator>Hagerman, Gordon F</creator><creator>Noel, Nicole C L</creator><creator>Cao, Sylvia Y</creator><creator>DuVal, Michèle G</creator><creator>Oel, A Phillip</creator><creator>Allison, W Ted</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20161128</creationdate><title>Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish</title><author>Hagerman, Gordon F ; Noel, Nicole C L ; Cao, Sylvia Y ; DuVal, Michèle G ; Oel, A Phillip ; Allison, W Ted</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-14228f21d4f263646ae32208ea205a7542b6b694e3bde5f30eafa97ee0f3e61c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Ablation (Surgery)</topic><topic>Ablation Techniques</topic><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Apoptosis</topic><topic>Biology and Life Sciences</topic><topic>Cell Death</topic><topic>Cell Proliferation</topic><topic>Cones</topic><topic>Danio rerio</topic><topic>Degeneration</topic><topic>Genetics</topic><topic>Larva</topic><topic>Larvae</topic><topic>Medicine and Health Sciences</topic><topic>Melanins - metabolism</topic><topic>Metronidazole - pharmacology</topic><topic>Nitroreductases - genetics</topic><topic>Nitroreductases - metabolism</topic><topic>Oncorhynchus mykiss</topic><topic>Photoreception</topic><topic>Photoreceptors</topic><topic>Physical Sciences</topic><topic>Physiological aspects</topic><topic>Pigmentation</topic><topic>Recovery</topic><topic>Recovery (Medical)</topic><topic>Recovery of function</topic><topic>Regeneration</topic><topic>Research and Analysis Methods</topic><topic>Retina</topic><topic>Retina - cytology</topic><topic>Retinal Cone Photoreceptor Cells - drug effects</topic><topic>Retinal Cone Photoreceptor Cells - physiology</topic><topic>Retinal Degeneration</topic><topic>Rewiring</topic><topic>Social Sciences</topic><topic>Stem cells</topic><topic>Thyroid gland</topic><topic>Trout</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><topic>Vision</topic><topic>Vision, Ocular - physiology</topic><topic>Visual perception</topic><topic>Zebrafish</topic><topic>Zebrafish - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hagerman, Gordon F</creatorcontrib><creatorcontrib>Noel, Nicole C L</creatorcontrib><creatorcontrib>Cao, Sylvia Y</creatorcontrib><creatorcontrib>DuVal, Michèle G</creatorcontrib><creatorcontrib>Oel, A Phillip</creatorcontrib><creatorcontrib>Allison, W Ted</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hagerman, Gordon F</au><au>Noel, Nicole C L</au><au>Cao, Sylvia Y</au><au>DuVal, Michèle G</au><au>Oel, A Phillip</au><au>Allison, W Ted</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-11-28</date><risdate>2016</risdate><volume>11</volume><issue>11</issue><spage>e0166932</spage><epage>e0166932</epage><pages>e0166932-e0166932</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hurdles in the treatment of retinal degeneration include managing the functional rewiring of surviving photoreceptors and integration of any newly added cells into the remaining second-order retinal neurons. Zebrafish are the premier genetic model for such questions, and we present two new transgenic lines allowing us to contrast vision loss and recovery following conditional ablation of specific cone types: UV or blue cones. The ablation of each cone type proved to be thorough (killing 80% of cells in each intended cone class), specific, and cell-autonomous. We assessed the loss and recovery of vision in larvae via the optomotor behavioural response (OMR). This visually mediated behaviour decreased to about 5% or 20% of control levels following ablation of UV or blue cones, respectively (P<0.05). We further assessed ocular photoreception by measuring the effects of UV light on body pigmentation, and observed that photoreceptor deficits and recovery occurred (p<0.01) with a timeline coincident to the OMR results. This corroborated and extended previous conclusions that UV cones are required photoreceptors for modulating body pigmentation, addressing assumptions that were unavoidable in previous experiments. Functional vision recovery following UV cone ablation was robust, as measured by both assays, returning to control levels within four days. In contrast, robust functional recovery following blue cone ablation was unexpectedly rapid, returning to normal levels within 24 hours after ablation. Ablation of cones led to increased proliferation in the retina, though the rapid recovery of vision following blue cone ablation was demonstrated to not be mediated by blue cone regeneration. Thus rapid visual recovery occurs following ablation of some, but not all, cone subtypes, suggesting an opportunity to contrast and dissect the sources and mechanisms of outer retinal recovery during cone photoreceptor death and regeneration.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27893779</pmid><doi>10.1371/journal.pone.0166932</doi><tpages>e0166932</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-11, Vol.11 (11), p.e0166932-e0166932 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1844332559 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Ablation (Surgery) Ablation Techniques Animals Animals, Genetically Modified Apoptosis Biology and Life Sciences Cell Death Cell Proliferation Cones Danio rerio Degeneration Genetics Larva Larvae Medicine and Health Sciences Melanins - metabolism Metronidazole - pharmacology Nitroreductases - genetics Nitroreductases - metabolism Oncorhynchus mykiss Photoreception Photoreceptors Physical Sciences Physiological aspects Pigmentation Recovery Recovery (Medical) Recovery of function Regeneration Research and Analysis Methods Retina Retina - cytology Retinal Cone Photoreceptor Cells - drug effects Retinal Cone Photoreceptor Cells - physiology Retinal Degeneration Rewiring Social Sciences Stem cells Thyroid gland Trout Ultraviolet radiation Ultraviolet Rays Vision Vision, Ocular - physiology Visual perception Zebrafish Zebrafish - genetics |
| title | Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T00%3A58%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20Recovery%20of%20Visual%20Function%20Associated%20with%20Blue%20Cone%20Ablation%20in%20Zebrafish&rft.jtitle=PloS%20one&rft.au=Hagerman,%20Gordon%20F&rft.date=2016-11-28&rft.volume=11&rft.issue=11&rft.spage=e0166932&rft.epage=e0166932&rft.pages=e0166932-e0166932&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0166932&rft_dat=%3Cgale_plos_%3EA471946473%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1844332559&rft_id=info:pmid/27893779&rft_galeid=A471946473&rft_doaj_id=oai_doaj_org_article_368d28fe86474c08a9f33cd824dbdc0f&rfr_iscdi=true |