Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation

For most sample types, the automation of RNA and DNA sample preparation workflows enables high throughput next-generation sequencing (NGS) library preparation. Greater adoption of small RNA (sRNA) sequencing has been hindered by high sample input requirements and inherent ligation side products form...

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Veröffentlicht in:	PloS one 2016-11, Vol.11 (11), p.e0167009-e0167009
Hauptverfasser:	Shore, Sabrina, Henderson, Jordana M, Lebedev, Alexandre, Salcedo, Michelle P, Zon, Gerald, McCaffrey, Anton P, Paul, Natasha, Hogrefe, Richard I
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Adapters Aptamers, Nucleotide Automation Bioinformatics Biology and life sciences Biomarkers Breast cancer Contaminants Deoxyribonucleic acid DNA DNA sequencing Gene Library Gene sequencing Genomics High-Throughput Nucleotide Sequencing Libraries Methods MicroRNAs Molecular biology Purification R&D Research & development Research and analysis methods Ribonucleic acid RNA RNA, Small Untranslated - chemistry RNA, Small Untranslated - genetics Sample preparation Technology application Workflow
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