Effect of Transplanting Various Concentrations of a Composite of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Hyaluronic Acid Hydrogel on Articular Cartilage Repair in a Rabbit Model
Mesenchymal stem cells (MSCs) are known to have therapeutic potential for cartilage repair. However, the optimal concentration of MSCs for cartilage repair remains unclear. Therefore, we aimed to explore the feasibility of cartilage repair by human umbilical cord blood-derived MSCs (hUCB-MSCs) and t...
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| creator | Park, Yong-Beom Ha, Chul-Won Kim, Jin-A Rhim, Ji-Heon Park, Yong-Geun Chung, Jun Young Lee, Han-Jun |
| description | Mesenchymal stem cells (MSCs) are known to have therapeutic potential for cartilage repair. However, the optimal concentration of MSCs for cartilage repair remains unclear. Therefore, we aimed to explore the feasibility of cartilage repair by human umbilical cord blood-derived MSCs (hUCB-MSCs) and to determine the optimal concentrations of the MSCs in a rabbit model.
Osteochondral defects were created in the trochlear groove of femur in 55 rabbits. Four experimental groups (11 rabbits/group) were treated by transplanting the composite of hUCB-MSCs and HA with various MSCs concentrations (0.1, 0.5, 1.0, and 1.5 x 107 cells/ml). One control group was left untreated. At 4, 8, and 16 weeks post-transplantation, the degree of cartilage repair was evaluated grossly and histologically.
Overall, transplanting hUCB-MSCs and HA hydrogel resulted in cartilage repair tissue with better quality than the control without transplantation (P = 0.015 in 0.1, P = 0.004 in 0.5, P = 0.004 in 1.0, P = 0.132 in 1.5 x 107 cells/ml). Interestingly, high cell concentration of hUCB-MSCs (1.5×107 cells/ml) was inferior to low cell concentrations (0.1, 0.5, and 1.0 x 107 cells/ml) in cartilage repair (P = 0.394,P = 0.041, P = 0.699, respectively). The 0.5 x 107 cells/ml group showed the highest cartilage repair score at 4, 8 and 16 weeks post transplantation, and followed by 0.1x107 cells/ml group or 1.0 x 107 cell/ml group.
The results of this study suggest that transplantation of the composite of hUCB-MSCs and HA is beneficial for cartilage repair. In addition, this study shows that optimal MSC concentration needs to be determined for better cartilage repair. |
| doi_str_mv | 10.1371/journal.pone.0165446 |
| format | Article |
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Osteochondral defects were created in the trochlear groove of femur in 55 rabbits. Four experimental groups (11 rabbits/group) were treated by transplanting the composite of hUCB-MSCs and HA with various MSCs concentrations (0.1, 0.5, 1.0, and 1.5 x 107 cells/ml). One control group was left untreated. At 4, 8, and 16 weeks post-transplantation, the degree of cartilage repair was evaluated grossly and histologically.
Overall, transplanting hUCB-MSCs and HA hydrogel resulted in cartilage repair tissue with better quality than the control without transplantation (P = 0.015 in 0.1, P = 0.004 in 0.5, P = 0.004 in 1.0, P = 0.132 in 1.5 x 107 cells/ml). Interestingly, high cell concentration of hUCB-MSCs (1.5×107 cells/ml) was inferior to low cell concentrations (0.1, 0.5, and 1.0 x 107 cells/ml) in cartilage repair (P = 0.394,P = 0.041, P = 0.699, respectively). The 0.5 x 107 cells/ml group showed the highest cartilage repair score at 4, 8 and 16 weeks post transplantation, and followed by 0.1x107 cells/ml group or 1.0 x 107 cell/ml group.
The results of this study suggest that transplantation of the composite of hUCB-MSCs and HA is beneficial for cartilage repair. In addition, this study shows that optimal MSC concentration needs to be determined for better cartilage repair.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0165446</identifier><identifier>PMID: 27824874</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accreditation ; Analysis ; Animals ; Biocompatibility ; Biology and Life Sciences ; Biomedical materials ; Blood ; Bone marrow ; Bone surgery ; Cartilage ; Cartilage (articular) ; Cartilage, Articular - drug effects ; Cartilage, Articular - physiology ; Cord blood ; Feasibility studies ; Femur ; Fetal Blood - physiology ; Health aspects ; Hospitals ; Humans ; Hyaluronic acid ; Hyaluronic Acid - therapeutic use ; Hydrogel, Polyethylene Glycol Dimethacrylate - therapeutic use ; Hydrogels ; Laboratory animals ; Male ; Medicine ; Medicine and Health Sciences ; Mesenchymal Stem Cell Transplantation - methods ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - physiology ; Mesenchyme ; Orthopedics ; Physical Sciences ; Rabbits ; Repair ; Research and Analysis Methods ; Skin & tissue grafts ; Stem cell transplantation ; Stem cells ; Studies ; Tissue Engineering - methods ; Transplantation ; Umbilical cord ; Wound Healing - physiology</subject><ispartof>PloS one, 2016-11, Vol.11 (11), p.e0165446-e0165446</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Park et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Park et al 2016 Park et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-118ba230ed74871a5d6600dbad905aeae13a1d696a0ca9d93a0f7786ab2f5c573</citedby><cites>FETCH-LOGICAL-c725t-118ba230ed74871a5d6600dbad905aeae13a1d696a0ca9d93a0f7786ab2f5c573</cites><orcidid>0000-0002-3741-2311</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100912/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100912/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23868,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27824874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kerkis, Irina</contributor><creatorcontrib>Park, Yong-Beom</creatorcontrib><creatorcontrib>Ha, Chul-Won</creatorcontrib><creatorcontrib>Kim, Jin-A</creatorcontrib><creatorcontrib>Rhim, Ji-Heon</creatorcontrib><creatorcontrib>Park, Yong-Geun</creatorcontrib><creatorcontrib>Chung, Jun Young</creatorcontrib><creatorcontrib>Lee, Han-Jun</creatorcontrib><title>Effect of Transplanting Various Concentrations of a Composite of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Hyaluronic Acid Hydrogel on Articular Cartilage Repair in a Rabbit Model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mesenchymal stem cells (MSCs) are known to have therapeutic potential for cartilage repair. However, the optimal concentration of MSCs for cartilage repair remains unclear. Therefore, we aimed to explore the feasibility of cartilage repair by human umbilical cord blood-derived MSCs (hUCB-MSCs) and to determine the optimal concentrations of the MSCs in a rabbit model.
Osteochondral defects were created in the trochlear groove of femur in 55 rabbits. Four experimental groups (11 rabbits/group) were treated by transplanting the composite of hUCB-MSCs and HA with various MSCs concentrations (0.1, 0.5, 1.0, and 1.5 x 107 cells/ml). One control group was left untreated. At 4, 8, and 16 weeks post-transplantation, the degree of cartilage repair was evaluated grossly and histologically.
Overall, transplanting hUCB-MSCs and HA hydrogel resulted in cartilage repair tissue with better quality than the control without transplantation (P = 0.015 in 0.1, P = 0.004 in 0.5, P = 0.004 in 1.0, P = 0.132 in 1.5 x 107 cells/ml). Interestingly, high cell concentration of hUCB-MSCs (1.5×107 cells/ml) was inferior to low cell concentrations (0.1, 0.5, and 1.0 x 107 cells/ml) in cartilage repair (P = 0.394,P = 0.041, P = 0.699, respectively). The 0.5 x 107 cells/ml group showed the highest cartilage repair score at 4, 8 and 16 weeks post transplantation, and followed by 0.1x107 cells/ml group or 1.0 x 107 cell/ml group.
The results of this study suggest that transplantation of the composite of hUCB-MSCs and HA is beneficial for cartilage repair. In addition, this study shows that optimal MSC concentration needs to be determined for better cartilage repair.</description><subject>Accreditation</subject><subject>Analysis</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biology and Life Sciences</subject><subject>Biomedical materials</subject><subject>Blood</subject><subject>Bone marrow</subject><subject>Bone surgery</subject><subject>Cartilage</subject><subject>Cartilage (articular)</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cartilage, Articular - physiology</subject><subject>Cord blood</subject><subject>Feasibility studies</subject><subject>Femur</subject><subject>Fetal Blood - physiology</subject><subject>Health aspects</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Hyaluronic acid</subject><subject>Hyaluronic Acid - therapeutic use</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - therapeutic use</subject><subject>Hydrogels</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mesenchyme</subject><subject>Orthopedics</subject><subject>Physical Sciences</subject><subject>Rabbits</subject><subject>Repair</subject><subject>Research and Analysis Methods</subject><subject>Skin & tissue grafts</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Tissue Engineering - methods</subject><subject>Transplantation</subject><subject>Umbilical cord</subject><subject>Wound Healing - physiology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk81u1DAQxyMEoqXwBggsISE47GI7iZ1ckJal0EqtKvXrak1sZ9eVY2_tpKJPyGvhdLdVF_VQ5RB7_Ju_58OTZe8JnpKck29XfggO7HTlnZ5iwsqiYC-yXVLndMIozl8-Wu9kb2K8wrjMK8ZeZzuUV7SoeLGb_d1vWy175Ft0HsDFlQXXG7dAlxCMHyKaeye16wP0xrs4cpBs3cpH0-txezB04NBF1xhrJNh0GBT6Yb1Xk586mBut0LGO2snlbZeOz3rdobm2NiJwCh3cgh2Cd0aimTTjXgW_0BZ5h2ahN3KwENAc0tLCQqNTvQITkHEpjFNoGtOjY6-0fZu9asFG_W7z38sufu2fzw8mRye_D-ezo4nktOwnhFQN0BxrxVP-BErFGMaqAVXjEjRokgNRrGaAJdSqzgG3nFcMGtqWsuT5XvZxrbuyPopND6IgVc4ppZiViThcE8rDlVgF00G4FR6MuDP4sBBjNtJqkbO6lYpVTSVT87gEzHPaUJo3BJqyIUnr--a2oem0WjfCbolunzizFAt_I0qCcU1oEviyEQj-etCxF52JMlUfnE7dTXEXrMApdv4MNGF1XdQsoZ_-Q58uxIZaQMrVuNanEOUoKmYFT2DFOU7U9AkqfUp3Rqa33Zpk33L4uuWQmF7_6RcwxCgOz06fz55cbrOfH7FLDbZfRm-Hu3e_DRZrUAYfY9DtQz8IFuNo3ldDjKMpNqOZ3D487uWD0_0s5v8AAAY10w</recordid><startdate>20161108</startdate><enddate>20161108</enddate><creator>Park, Yong-Beom</creator><creator>Ha, Chul-Won</creator><creator>Kim, Jin-A</creator><creator>Rhim, Ji-Heon</creator><creator>Park, Yong-Geun</creator><creator>Chung, Jun Young</creator><creator>Lee, Han-Jun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3741-2311</orcidid></search><sort><creationdate>20161108</creationdate><title>Effect of Transplanting Various Concentrations of a Composite of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Hyaluronic Acid Hydrogel on Articular Cartilage Repair in a Rabbit Model</title><author>Park, Yong-Beom ; Ha, Chul-Won ; Kim, Jin-A ; Rhim, Ji-Heon ; Park, Yong-Geun ; Chung, Jun Young ; Lee, Han-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-118ba230ed74871a5d6600dbad905aeae13a1d696a0ca9d93a0f7786ab2f5c573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Accreditation</topic><topic>Analysis</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biology and Life Sciences</topic><topic>Biomedical materials</topic><topic>Blood</topic><topic>Bone marrow</topic><topic>Bone surgery</topic><topic>Cartilage</topic><topic>Cartilage (articular)</topic><topic>Cartilage, Articular - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Yong-Beom</au><au>Ha, Chul-Won</au><au>Kim, Jin-A</au><au>Rhim, Ji-Heon</au><au>Park, Yong-Geun</au><au>Chung, Jun Young</au><au>Lee, Han-Jun</au><au>Kerkis, Irina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Transplanting Various Concentrations of a Composite of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Hyaluronic Acid Hydrogel on Articular Cartilage Repair in a Rabbit Model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-11-08</date><risdate>2016</risdate><volume>11</volume><issue>11</issue><spage>e0165446</spage><epage>e0165446</epage><pages>e0165446-e0165446</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mesenchymal stem cells (MSCs) are known to have therapeutic potential for cartilage repair. However, the optimal concentration of MSCs for cartilage repair remains unclear. Therefore, we aimed to explore the feasibility of cartilage repair by human umbilical cord blood-derived MSCs (hUCB-MSCs) and to determine the optimal concentrations of the MSCs in a rabbit model.
Osteochondral defects were created in the trochlear groove of femur in 55 rabbits. Four experimental groups (11 rabbits/group) were treated by transplanting the composite of hUCB-MSCs and HA with various MSCs concentrations (0.1, 0.5, 1.0, and 1.5 x 107 cells/ml). One control group was left untreated. At 4, 8, and 16 weeks post-transplantation, the degree of cartilage repair was evaluated grossly and histologically.
Overall, transplanting hUCB-MSCs and HA hydrogel resulted in cartilage repair tissue with better quality than the control without transplantation (P = 0.015 in 0.1, P = 0.004 in 0.5, P = 0.004 in 1.0, P = 0.132 in 1.5 x 107 cells/ml). Interestingly, high cell concentration of hUCB-MSCs (1.5×107 cells/ml) was inferior to low cell concentrations (0.1, 0.5, and 1.0 x 107 cells/ml) in cartilage repair (P = 0.394,P = 0.041, P = 0.699, respectively). The 0.5 x 107 cells/ml group showed the highest cartilage repair score at 4, 8 and 16 weeks post transplantation, and followed by 0.1x107 cells/ml group or 1.0 x 107 cell/ml group.
The results of this study suggest that transplantation of the composite of hUCB-MSCs and HA is beneficial for cartilage repair. In addition, this study shows that optimal MSC concentration needs to be determined for better cartilage repair.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27824874</pmid><doi>10.1371/journal.pone.0165446</doi><tpages>e0165446</tpages><orcidid>https://orcid.org/0000-0002-3741-2311</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-11, Vol.11 (11), p.e0165446-e0165446 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1837222065 |
| source | PubMed Central Free; MEDLINE; Public Library of Science; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; EZB Electronic Journals Library |
| subjects | Accreditation Analysis Animals Biocompatibility Biology and Life Sciences Biomedical materials Blood Bone marrow Bone surgery Cartilage Cartilage (articular) Cartilage, Articular - drug effects Cartilage, Articular - physiology Cord blood Feasibility studies Femur Fetal Blood - physiology Health aspects Hospitals Humans Hyaluronic acid Hyaluronic Acid - therapeutic use Hydrogel, Polyethylene Glycol Dimethacrylate - therapeutic use Hydrogels Laboratory animals Male Medicine Medicine and Health Sciences Mesenchymal Stem Cell Transplantation - methods Mesenchymal stem cells Mesenchymal Stromal Cells - physiology Mesenchyme Orthopedics Physical Sciences Rabbits Repair Research and Analysis Methods Skin & tissue grafts Stem cell transplantation Stem cells Studies Tissue Engineering - methods Transplantation Umbilical cord Wound Healing - physiology |
| title | Effect of Transplanting Various Concentrations of a Composite of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Hyaluronic Acid Hydrogel on Articular Cartilage Repair in a Rabbit Model |
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