Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells
Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medic...
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| Veröffentlicht in: | PloS one 2016-11, Vol.11 (11), p.e0165715 |
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| creator | Chang, Wei-Fang Hwu, Yuh-Ming Xu, Jie Lin, Chen-Ju Wang, Sheng-Wen Cheng, An-Sheng Lu, Jean Lu, Chung-Hao Sung, Li-Ying |
| description | Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medicine. In this study, we derived patient-specific iPSCs using CCs from human infertility clinics for the first time. The human cumulus cell derived iPSCs (hc-iPSCs) were characterized for growth, karyotype, expression of pluripotency genes, and were subjected to embryoid bodies (EBs) and teratoma assays to evaluate their differentiation capacity. Hc-iPSCs display typical iPSC characteristics, and are capable of differentiating into all germ layers in vitro and in vivo. We further show that putative primordial germ cell like cells (PGCLCs) can be derived using hc-iPSCs. Our data demonstrate the feasibility of deriving patient-specific pluripotent stem cells using CCs. |
| doi_str_mv | 10.1371/journal.pone.0165715 |
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In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medicine. In this study, we derived patient-specific iPSCs using CCs from human infertility clinics for the first time. The human cumulus cell derived iPSCs (hc-iPSCs) were characterized for growth, karyotype, expression of pluripotency genes, and were subjected to embryoid bodies (EBs) and teratoma assays to evaluate their differentiation capacity. Hc-iPSCs display typical iPSC characteristics, and are capable of differentiating into all germ layers in vitro and in vivo. We further show that putative primordial germ cell like cells (PGCLCs) can be derived using hc-iPSCs. Our data demonstrate the feasibility of deriving patient-specific pluripotent stem cells using CCs.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0165715</identifier><identifier>PMID: 27802323</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Agricultural biotechnology ; Biological materials ; Biology and Life Sciences ; Cell Differentiation ; Chromosomes ; Chromosomes, Human, X - genetics ; Cloning ; Community colleges ; Cumulus Cells - cytology ; Embryos ; Feasibility studies ; Female ; Fibroblasts ; Gene expression ; Gene Expression Regulation ; Germ Layers - cytology ; Growth factors ; Gynecology ; Hospitals ; Humans ; In vitro fertilization ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - metabolism ; Infertility ; Medicine ; Medicine and Health Sciences ; Obstetrics ; Patients ; Pluripotency ; Regenerative medicine ; Reproductive technologies ; Research and Analysis Methods ; Stem cell research ; Stem cells ; Teratoma</subject><ispartof>PloS one, 2016-11, Vol.11 (11), p.e0165715</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Chang et al 2016 Chang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c795t-d9636e7096eaf59cc68ba7d4f7696c00a70f02bb2871d01f9a38ae34f8b9f2b03</citedby><cites>FETCH-LOGICAL-c795t-d9636e7096eaf59cc68ba7d4f7696c00a70f02bb2871d01f9a38ae34f8b9f2b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089679/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089679/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23849,27907,27908,53774,53776,79351,79352</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27802323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhou, Yunli</contributor><creatorcontrib>Chang, Wei-Fang</creatorcontrib><creatorcontrib>Hwu, Yuh-Ming</creatorcontrib><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Lin, Chen-Ju</creatorcontrib><creatorcontrib>Wang, Sheng-Wen</creatorcontrib><creatorcontrib>Cheng, An-Sheng</creatorcontrib><creatorcontrib>Lu, Jean</creatorcontrib><creatorcontrib>Lu, Chung-Hao</creatorcontrib><creatorcontrib>Sung, Li-Ying</creatorcontrib><title>Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. 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Our data demonstrate the feasibility of deriving patient-specific pluripotent stem cells using CCs.</description><subject>Adult</subject><subject>Agricultural biotechnology</subject><subject>Biological materials</subject><subject>Biology and Life Sciences</subject><subject>Cell Differentiation</subject><subject>Chromosomes</subject><subject>Chromosomes, Human, X - genetics</subject><subject>Cloning</subject><subject>Community colleges</subject><subject>Cumulus Cells - cytology</subject><subject>Embryos</subject><subject>Feasibility studies</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Germ Layers - cytology</subject><subject>Growth factors</subject><subject>Gynecology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>In vitro fertilization</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - 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Yuh-Ming</au><au>Xu, Jie</au><au>Lin, Chen-Ju</au><au>Wang, Sheng-Wen</au><au>Cheng, An-Sheng</au><au>Lu, Jean</au><au>Lu, Chung-Hao</au><au>Sung, Li-Ying</au><au>Zhou, Yunli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>11</volume><issue>11</issue><spage>e0165715</spage><pages>e0165715-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. 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Our data demonstrate the feasibility of deriving patient-specific pluripotent stem cells using CCs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27802323</pmid><doi>10.1371/journal.pone.0165715</doi><tpages>e0165715</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Agricultural biotechnology Biological materials Biology and Life Sciences Cell Differentiation Chromosomes Chromosomes, Human, X - genetics Cloning Community colleges Cumulus Cells - cytology Embryos Feasibility studies Female Fibroblasts Gene expression Gene Expression Regulation Germ Layers - cytology Growth factors Gynecology Hospitals Humans In vitro fertilization Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Infertility Medicine Medicine and Health Sciences Obstetrics Patients Pluripotency Regenerative medicine Reproductive technologies Research and Analysis Methods Stem cell research Stem cells Teratoma |
| title | Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells |
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