Cold Atmospheric Plasma Inhibits HIV-1 Replication in Macrophages by Targeting Both the Virus and the Cells
Cold atmospheric plasma (CAP) is a specific type of partially ionized gas that is less than 104°F at the point of application. It was recently shown that CAP can be used for decontamination and sterilization, as well as anti-cancer treatment. Here, we investigated the effects of CAP on HIV-1 replica...
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| creator | Volotskova, Olga Dubrovsky, Larisa Keidar, Michael Bukrinsky, Michael |
| description | Cold atmospheric plasma (CAP) is a specific type of partially ionized gas that is less than 104°F at the point of application. It was recently shown that CAP can be used for decontamination and sterilization, as well as anti-cancer treatment. Here, we investigated the effects of CAP on HIV-1 replication in monocyte-derived macrophages (MDM). We demonstrate that pre-treatment of MDM with CAP reduced levels of CD4 and CCR5, inhibiting virus-cell fusion, viral reverse transcription and integration. In addition, CAP pre-treatment affected cellular factors required for post-entry events, as replication of VSV-G-pseudotyped HIV-1, which by-passes HIV receptor-mediated fusion at the plasma membrane during entry, was also inhibited. Interestingly, virus particles produced by CAP-treated cells had reduced infectivity, suggesting that the inhibitory effect of CAP extended to the second cycle of infection. These results demonstrate that anti-HIV activity of CAP involves the effects on target cells and the virus, and suggest that CAP may be considered for potential application as an anti-HIV treatment. |
| doi_str_mv | 10.1371/journal.pone.0165322 |
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It was recently shown that CAP can be used for decontamination and sterilization, as well as anti-cancer treatment. Here, we investigated the effects of CAP on HIV-1 replication in monocyte-derived macrophages (MDM). We demonstrate that pre-treatment of MDM with CAP reduced levels of CD4 and CCR5, inhibiting virus-cell fusion, viral reverse transcription and integration. In addition, CAP pre-treatment affected cellular factors required for post-entry events, as replication of VSV-G-pseudotyped HIV-1, which by-passes HIV receptor-mediated fusion at the plasma membrane during entry, was also inhibited. Interestingly, virus particles produced by CAP-treated cells had reduced infectivity, suggesting that the inhibitory effect of CAP extended to the second cycle of infection. 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Volotskova et al 2016 Volotskova et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c795t-8383268d52e8e65bfd8aaadf95c8f6beef6dd19861f3fd20aa051523dad5b08c3</citedby><cites>FETCH-LOGICAL-c795t-8383268d52e8e65bfd8aaadf95c8f6beef6dd19861f3fd20aa051523dad5b08c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081187/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5081187/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27783659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Harrich, David</contributor><creatorcontrib>Volotskova, Olga</creatorcontrib><creatorcontrib>Dubrovsky, Larisa</creatorcontrib><creatorcontrib>Keidar, Michael</creatorcontrib><creatorcontrib>Bukrinsky, Michael</creatorcontrib><title>Cold Atmospheric Plasma Inhibits HIV-1 Replication in Macrophages by Targeting Both the Virus and the Cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cold atmospheric plasma (CAP) is a specific type of partially ionized gas that is less than 104°F at the point of application. It was recently shown that CAP can be used for decontamination and sterilization, as well as anti-cancer treatment. Here, we investigated the effects of CAP on HIV-1 replication in monocyte-derived macrophages (MDM). We demonstrate that pre-treatment of MDM with CAP reduced levels of CD4 and CCR5, inhibiting virus-cell fusion, viral reverse transcription and integration. In addition, CAP pre-treatment affected cellular factors required for post-entry events, as replication of VSV-G-pseudotyped HIV-1, which by-passes HIV receptor-mediated fusion at the plasma membrane during entry, was also inhibited. Interestingly, virus particles produced by CAP-treated cells had reduced infectivity, suggesting that the inhibitory effect of CAP extended to the second cycle of infection. These results demonstrate that anti-HIV activity of CAP involves the effects on target cells and the virus, and suggest that CAP may be considered for potential application as an anti-HIV treatment.</description><subject>Aerospace engineering</subject><subject>AIDS treatment</subject><subject>Antiviral activity</subject><subject>Atmospheric Pressure</subject><subject>Biology and Life Sciences</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>CCR5 protein</subject><subject>CD4 antigen</subject><subject>CD4 Antigens - metabolism</subject><subject>Cell cycle</subject><subject>Cell fusion</subject><subject>Cells, Cultured</subject><subject>Charged particles</subject><subject>Cold</subject><subject>Decontamination</subject><subject>Health aspects</subject><subject>Helium - chemistry</subject><subject>Helium - pharmacology</subject><subject>Herpes viruses</subject><subject>HIV</subject><subject>HIV-1 - physiology</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunology</subject><subject>Infectivity</subject><subject>Lentivirus</subject><subject>Macrophages</subject><subject>Macrophages - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Volotskova, Olga</au><au>Dubrovsky, Larisa</au><au>Keidar, Michael</au><au>Bukrinsky, Michael</au><au>Harrich, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cold Atmospheric Plasma Inhibits HIV-1 Replication in Macrophages by Targeting Both the Virus and the Cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-10-26</date><risdate>2016</risdate><volume>11</volume><issue>10</issue><spage>e0165322</spage><epage>e0165322</epage><pages>e0165322-e0165322</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cold atmospheric plasma (CAP) is a specific type of partially ionized gas that is less than 104°F at the point of application. It was recently shown that CAP can be used for decontamination and sterilization, as well as anti-cancer treatment. Here, we investigated the effects of CAP on HIV-1 replication in monocyte-derived macrophages (MDM). We demonstrate that pre-treatment of MDM with CAP reduced levels of CD4 and CCR5, inhibiting virus-cell fusion, viral reverse transcription and integration. In addition, CAP pre-treatment affected cellular factors required for post-entry events, as replication of VSV-G-pseudotyped HIV-1, which by-passes HIV receptor-mediated fusion at the plasma membrane during entry, was also inhibited. Interestingly, virus particles produced by CAP-treated cells had reduced infectivity, suggesting that the inhibitory effect of CAP extended to the second cycle of infection. These results demonstrate that anti-HIV activity of CAP involves the effects on target cells and the virus, and suggest that CAP may be considered for potential application as an anti-HIV treatment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27783659</pmid><doi>10.1371/journal.pone.0165322</doi><tpages>e0165322</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aerospace engineering AIDS treatment Antiviral activity Atmospheric Pressure Biology and Life Sciences Cancer Cancer therapies CCR5 protein CD4 antigen CD4 Antigens - metabolism Cell cycle Cell fusion Cells, Cultured Charged particles Cold Decontamination Health aspects Helium - chemistry Helium - pharmacology Herpes viruses HIV HIV-1 - physiology Human immunodeficiency virus Humans Immunology Infectivity Lentivirus Macrophages Macrophages - cytology Macrophages - drug effects Macrophages - metabolism Macrophages - virology Medicine and Health Sciences Membrane fusion Monocytes Monocytes - cytology Plasma Plasma Gases - chemistry Plasma Gases - pharmacology Receptors, CCR5 - metabolism Replication Retroviridae Reverse transcription Sterilization Virus replication Virus Replication - drug effects Viruses |
| title | Cold Atmospheric Plasma Inhibits HIV-1 Replication in Macrophages by Targeting Both the Virus and the Cells |
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