Increased Abundance of Proteins Involved in Resistance to Oxidative and Nitrosative Stress at the Last Stages of Growth and Development of Leishmania amazonensis Promastigotes Revealed by Proteome Analysis
Leishmania amazonensis is one of the major etiological agents of the neglected, stigmatizing disease termed american cutaneous leishmaniasis (ACL). ACL is a zoonosis and rodents are the main reservoirs. Most cases of ACL are reported in Brazil, Bolivia, Colombia and Peru. The biological cycle of the...
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| description | Leishmania amazonensis is one of the major etiological agents of the neglected, stigmatizing disease termed american cutaneous leishmaniasis (ACL). ACL is a zoonosis and rodents are the main reservoirs. Most cases of ACL are reported in Brazil, Bolivia, Colombia and Peru. The biological cycle of the parasite is digenetic because sand fly vectors transmit the motile promastigote stage to the mammalian host dermis during blood meal intakes. The amastigote stage survives within phagocytes of the mammalian host. The purpose of this study is detection and identification of changes in protein abundance by 2DE/MALDI-TOF/TOF at the main growth phases of L. amazonensis promastigotes in axenic culture and the differentiation process that takes place simultaneously. The average number of proteins detected per gel is 202 and the non-redundant cumulative number is 339. Of those, 63 are differentially abundant throughout growth and simultaneous differentiation of L. amazonensis promastigotes. The main finding is that certain proteins involved in resistance to nitrosative and oxidative stress are more abundant at the last stages of growth and differentiation of cultured L. amazonensis promastigotes. These proteins are the arginase, a light variant of the tryparedoxin peroxidase, the iron superoxide dismutase, the regulatory subunit of the protein kinase A and a light HSP70 variant. These data taken together with the decrease of the stress-inducible protein 1 levels are additional evidence supporting the previously described pre-adaptative hypothesis, which consists of preparation in advance towards the amastigote stage. |
| doi_str_mv | 10.1371/journal.pone.0164344 |
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ACL is a zoonosis and rodents are the main reservoirs. Most cases of ACL are reported in Brazil, Bolivia, Colombia and Peru. The biological cycle of the parasite is digenetic because sand fly vectors transmit the motile promastigote stage to the mammalian host dermis during blood meal intakes. The amastigote stage survives within phagocytes of the mammalian host. The purpose of this study is detection and identification of changes in protein abundance by 2DE/MALDI-TOF/TOF at the main growth phases of L. amazonensis promastigotes in axenic culture and the differentiation process that takes place simultaneously. The average number of proteins detected per gel is 202 and the non-redundant cumulative number is 339. Of those, 63 are differentially abundant throughout growth and simultaneous differentiation of L. amazonensis promastigotes. The main finding is that certain proteins involved in resistance to nitrosative and oxidative stress are more abundant at the last stages of growth and differentiation of cultured L. amazonensis promastigotes. These proteins are the arginase, a light variant of the tryparedoxin peroxidase, the iron superoxide dismutase, the regulatory subunit of the protein kinase A and a light HSP70 variant. These data taken together with the decrease of the stress-inducible protein 1 levels are additional evidence supporting the previously described pre-adaptative hypothesis, which consists of preparation in advance towards the amastigote stage.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0164344</identifier><identifier>PMID: 27776144</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abundance ; Arginase ; Biology and Life Sciences ; Culture techniques ; Cutaneous leishmaniasis ; Dermis ; Differentiation ; Electrophoresis, Gel, Two-Dimensional ; Etiology ; Gene expression ; Genomics ; Growth ; Hsp70 protein ; Immunology ; Iron ; Leishmania ; Leishmania - genetics ; Leishmania - metabolism ; Leishmania amazonensis ; Leishmania infantum ; Leishmania mexicana ; Leishmaniasis ; Mammals ; Nitric oxide ; Nitrosation ; Oxidation resistance ; Oxidative Stress ; Parasites ; Parasitic diseases ; Peroxidase ; Phagocytes ; Promastigotes ; Protein kinase A ; Protein kinases ; Proteins ; Proteolysis ; Proteome ; Proteomes ; Protozoan Proteins - metabolism ; Pure culture ; Rodents ; Signal Transduction ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Superoxide dismutase ; Tryparedoxin peroxidase ; Up-Regulation ; Vector-borne diseases ; Vectors ; Zoonoses</subject><ispartof>PloS one, 2016-10, Vol.11 (10), p.e0164344-e0164344</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Alcolea et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Alcolea et al 2016 Alcolea et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-bce22ac3a98044e4136a66e5a66389eb649bb98881af66536e07ec79b1b14d0e3</citedby><cites>FETCH-LOGICAL-c725t-bce22ac3a98044e4136a66e5a66389eb649bb98881af66536e07ec79b1b14d0e3</cites><orcidid>0000-0002-0729-8941</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5077082/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5077082/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27776144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Stäger, Simona</contributor><creatorcontrib>Alcolea, Pedro J</creatorcontrib><creatorcontrib>Alonso, Ana</creatorcontrib><creatorcontrib>García-Tabares, Francisco</creatorcontrib><creatorcontrib>Mena, María C</creatorcontrib><creatorcontrib>Ciordia, Sergio</creatorcontrib><creatorcontrib>Larraga, Vicente</creatorcontrib><title>Increased Abundance of Proteins Involved in Resistance to Oxidative and Nitrosative Stress at the Last Stages of Growth and Development of Leishmania amazonensis Promastigotes Revealed by Proteome Analysis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Leishmania amazonensis is one of the major etiological agents of the neglected, stigmatizing disease termed american cutaneous leishmaniasis (ACL). 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metabolism</subject><subject>Pure culture</subject><subject>Rodents</subject><subject>Signal Transduction</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Superoxide dismutase</subject><subject>Tryparedoxin peroxidase</subject><subject>Up-Regulation</subject><subject>Vector-borne diseases</subject><subject>Vectors</subject><subject>Zoonoses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0WLHju3cIFUDRqWKoQ24tRzntPWU2J3tlI3_yH_CabtpRbuYIuXDfs7rN-cjy15iNMaE4w8XrvdWteOVszBGmFFC6aPsEFekGLECkcd33g-yZyFcIFQSwdjT7KDgnDNM6WH2d2q1BxWgySd1bxtlNeRunn_3LoKxIZ_atWvXadvY_AyCCXGDRJefXplGRbOGXNkm_2aid2H7fR49hJCrmMcl5DMVYlpSCwiD8ol3v-NyE_MJ1tC6VQc2DjszMGHZKWtUrjr1J_2XTecNVrokYRbJUUge1qDa5Ke-3pp0HeSTlIjrxD7PnsxVG-DF7nmU_fzy-cfx19Hs9GR6PJmNNC_KOKo1FIXSRFUCUQoUE6YYgzLdiKigZrSq60oIgdWcsZIwQBw0r2pcY9ogIEfZ663uqnVB7ioRJBakQFiIEiViuiUapy7kyptO-WvplJGbBecXUvlodAuyFroRSFWa1w0tgVa6KignZc0xbkDrpPVxd1pfd9DolC6v2j3R_R1rlnLh1rJEnCNRJIF3OwHvLnsIUXYmaGhbZcH1G9-cFIylfnkAWpZY0GJA3_yH3p-IHbVIVZPGzl2yqAdROaEcV6jggiVqfA-VrgY6o1MnzE1a3wt4vxeQmAhXcaH6EOT0_Ozh7OmvffbtHXaZWi0ug2v7aJwN-yDdgjq1ffAwv60HRnIY0JtsyGFA5W5AU9iru7W8DbqZSPIPM2M5xw</recordid><startdate>20161024</startdate><enddate>20161024</enddate><creator>Alcolea, Pedro J</creator><creator>Alonso, Ana</creator><creator>García-Tabares, Francisco</creator><creator>Mena, María C</creator><creator>Ciordia, Sergio</creator><creator>Larraga, Vicente</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0729-8941</orcidid></search><sort><creationdate>20161024</creationdate><title>Increased Abundance of Proteins Involved in Resistance to Oxidative and Nitrosative Stress at the Last Stages of Growth and Development of Leishmania amazonensis Promastigotes Revealed by Proteome Analysis</title><author>Alcolea, Pedro J ; Alonso, Ana ; García-Tabares, Francisco ; Mena, María C ; Ciordia, Sergio ; Larraga, Vicente</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-bce22ac3a98044e4136a66e5a66389eb649bb98881af66536e07ec79b1b14d0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Abundance</topic><topic>Arginase</topic><topic>Biology and Life Sciences</topic><topic>Culture techniques</topic><topic>Cutaneous leishmaniasis</topic><topic>Dermis</topic><topic>Differentiation</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Etiology</topic><topic>Gene expression</topic><topic>Genomics</topic><topic>Growth</topic><topic>Hsp70 protein</topic><topic>Immunology</topic><topic>Iron</topic><topic>Leishmania</topic><topic>Leishmania - 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ACL is a zoonosis and rodents are the main reservoirs. Most cases of ACL are reported in Brazil, Bolivia, Colombia and Peru. The biological cycle of the parasite is digenetic because sand fly vectors transmit the motile promastigote stage to the mammalian host dermis during blood meal intakes. The amastigote stage survives within phagocytes of the mammalian host. The purpose of this study is detection and identification of changes in protein abundance by 2DE/MALDI-TOF/TOF at the main growth phases of L. amazonensis promastigotes in axenic culture and the differentiation process that takes place simultaneously. The average number of proteins detected per gel is 202 and the non-redundant cumulative number is 339. Of those, 63 are differentially abundant throughout growth and simultaneous differentiation of L. amazonensis promastigotes. The main finding is that certain proteins involved in resistance to nitrosative and oxidative stress are more abundant at the last stages of growth and differentiation of cultured L. amazonensis promastigotes. These proteins are the arginase, a light variant of the tryparedoxin peroxidase, the iron superoxide dismutase, the regulatory subunit of the protein kinase A and a light HSP70 variant. These data taken together with the decrease of the stress-inducible protein 1 levels are additional evidence supporting the previously described pre-adaptative hypothesis, which consists of preparation in advance towards the amastigote stage.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27776144</pmid><doi>10.1371/journal.pone.0164344</doi><tpages>e0164344</tpages><orcidid>https://orcid.org/0000-0002-0729-8941</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1832018850 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abundance Arginase Biology and Life Sciences Culture techniques Cutaneous leishmaniasis Dermis Differentiation Electrophoresis, Gel, Two-Dimensional Etiology Gene expression Genomics Growth Hsp70 protein Immunology Iron Leishmania Leishmania - genetics Leishmania - metabolism Leishmania amazonensis Leishmania infantum Leishmania mexicana Leishmaniasis Mammals Nitric oxide Nitrosation Oxidation resistance Oxidative Stress Parasites Parasitic diseases Peroxidase Phagocytes Promastigotes Protein kinase A Protein kinases Proteins Proteolysis Proteome Proteomes Protozoan Proteins - metabolism Pure culture Rodents Signal Transduction Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Superoxide dismutase Tryparedoxin peroxidase Up-Regulation Vector-borne diseases Vectors Zoonoses |
| title | Increased Abundance of Proteins Involved in Resistance to Oxidative and Nitrosative Stress at the Last Stages of Growth and Development of Leishmania amazonensis Promastigotes Revealed by Proteome Analysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A11%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20Abundance%20of%20Proteins%20Involved%20in%20Resistance%20to%20Oxidative%20and%20Nitrosative%20Stress%20at%20the%20Last%20Stages%20of%20Growth%20and%20Development%20of%20Leishmania%20amazonensis%20Promastigotes%20Revealed%20by%20Proteome%20Analysis&rft.jtitle=PloS%20one&rft.au=Alcolea,%20Pedro%20J&rft.date=2016-10-24&rft.volume=11&rft.issue=10&rft.spage=e0164344&rft.epage=e0164344&rft.pages=e0164344-e0164344&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0164344&rft_dat=%3Cgale_plos_%3EA471902786%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1832018850&rft_id=info:pmid/27776144&rft_galeid=A471902786&rft_doaj_id=oai_doaj_org_article_b8cd80a9c7bd45e49c924735b711decc&rfr_iscdi=true |