A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Most Relevant Members of the B. pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensis

The Burkholderia pseudomallei complex classically consisted of B. mallei, B. pseudomallei, and B. thailandensis, but has now expanded to include B. oklahomensis, B. humptydooensis, and three unassigned Burkholderia clades. Methods for detecting and differentiating the B. pseudomallei complex has bee...

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Veröffentlicht in:	PloS one 2016-10, Vol.11 (10), p.e0164006-e0164006
Hauptverfasser:	Lowe, Chinn-Woan, Satterfield, Benjamin A, Nelson, Daniel B, Thiriot, Joseph D, Heder, Michael J, March, Jordon K, Drake, David S, Lew, Cynthia S, Bunnell, Annette J, Moore, Emily S, O'Neill, Kim L, Robison, Richard A
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Bacteria Biology and Life Sciences Burkholderia Burkholderia - genetics Burkholderia - isolation & purification Burkholderia Infections - diagnosis Burkholderia Infections - microbiology Burkholderia mallei Burkholderia mallei - genetics Burkholderia mallei - isolation & purification Burkholderia pseudomallei Burkholderia pseudomallei - genetics Burkholderia pseudomallei - isolation & purification Differentiation DNA, Bacterial - isolation & purification Fatalities Glanders Glanders - microbiology Humans Identification methods Infections Infectious diseases Laboratories Medicine and Health Sciences Melioidosis Melioidosis - microbiology Molecular biology Oryza Pathogens Polymerase chain reaction Real time Real-Time Polymerase Chain Reaction - methods Research and Analysis Methods Sensitivity analysis Sensitivity and Specificity Sequence Analysis, DNA - methods Species
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