Dynamic ASXL1 Exon Skipping and Alternative Circular Splicing in Single Human Cells

Circular RNAs comprise a poorly understood new class of noncoding RNA. In this study, we used a combination of targeted deletion, high-resolution splicing detection, and single-cell sequencing to deeply probe ASXL1 circular splicing. We found that efficient circular splicing required the canonical t...

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Veröffentlicht in:	PloS one 2016-10, Vol.11 (10), p.e0164085-e0164085
Hauptverfasser:	Koh, Winston, Gonzalez, Veronica, Natarajan, Sivaraman, Carter, Robert, Brown, Patrick O, Gawad, Charles
Format:	Artikel
Sprache:	eng
Schlagworte:	Alternative Splicing Antisense RNA Bioengineering Biology and life sciences Biosynthesis Cells (Biology) Cellular biology Circularity Clonal deletion Cloning Competition Exon skipping Exons Gene deletion Gene expression HeLa Cells High-Throughput Nucleotide Sequencing Humans Interrogation Isoforms Oncology Plasmids Polymerase Chain Reaction Proteins Repressor Proteins - genetics Research and analysis methods Ribonucleic acid RNA RNA - genetics RNA Precursors - genetics Single-Cell Analysis Transcription
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