Method Specific Calibration Corrects for DNA Extraction Method Effects on Relative Telomere Length Measurements by Quantitative PCR

Telomere length (TL) is increasingly being used as a biomarker in epidemiological, biomedical and ecological studies. A wide range of DNA extraction techniques have been used in telomere experiments and recent quantitative PCR (qPCR) based studies suggest that the choice of DNA extraction method may...

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Veröffentlicht in:	PloS one 2016-10, Vol.11 (10), p.e0164046-e0164046
Hauptverfasser:	Seeker, Luise A, Holland, Rebecca, Underwood, Sarah, Fairlie, Jennifer, Psifidi, Androniki, Ilska, Joanna J, Bagnall, Ainsley, Whitelaw, Bruce, Coffey, Mike, Banos, Georgios, Nussey, Daniel H
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging Animals Beef cattle Biology and Life Sciences Biomarkers Blood Calibration Cattle Coefficient of variation Correlation analysis Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA - isolation & purification Ecological monitoring Ecological studies Engineering and Technology Epidemiology Evolutionary biology Female Genetic testing Historical account Internet Leukocytes Male Mathematical analysis Measurement Measurement methods Methods Phenols Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Research and analysis methods Researchers Salting Sheep Silica Silicon dioxide Statistical analysis Statistical methods Studies Telomere - genetics Telomere Homeostasis Telomeres
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