Complete Protection against Influenza Virus H1N1 Strain A/PR/8/34 Challenge in Mice Immunized with Non-Adjuvanted Novirhabdovirus Vaccines
Novirhabdoviruses like Viral Hemorrhagic Septicemia Virus (VHSV) and Infectious Hematopoietic Necrosis Virus (IHNV) are fish-infecting Rhabdoviruses belonging to the Mononegavirales order. By reverse genetics, we previously showed that a recombinant VHSV expressing the West Nile Virus (WNV) E glycop...
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| description | Novirhabdoviruses like Viral Hemorrhagic Septicemia Virus (VHSV) and Infectious Hematopoietic Necrosis Virus (IHNV) are fish-infecting Rhabdoviruses belonging to the Mononegavirales order. By reverse genetics, we previously showed that a recombinant VHSV expressing the West Nile Virus (WNV) E glycoprotein could serve as a vaccine platform against WNV. In the current study, we aimed to evaluate the potential of the Novirhabdovirus platform as a vaccine against influenza virus. Recombinant Novirhabdoviruses, rVHSV-HA and rIHNV-HA, expressing at the viral surface the hemagglutinin HA ectodomain were generated and used to immunized mice. We showed that mice immunized with either, rVHSV-HA or rIHNV-HA, elicited a strong neutralizing antibody response against influenza virus. A complete protection was conferred to the immunized mice when challenged with a lethal dose of influenza H1N1 A/PR/8/34 virus. Furthermore we showed that although acting as inert antigen in mice, since naturally inactivated over 20°C, mice immunized with rVHSV-HA or rIHNV-HA in the absence of adjuvant were also completely protected from a lethal challenge. Novirhabdoviruses platform are of particular interest as vaccines for mammals since they are cost effective to produce, relatively easy to generate and very effective to protect immunized animals. |
| doi_str_mv | 10.1371/journal.pone.0164245 |
| format | Article |
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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rouxel, Ronan N</au><au>Mérour, Emilie</au><au>Biacchesi, Stéphane</au><au>Brémont, Michel</au><au>Tsuji, Moriya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete Protection against Influenza Virus H1N1 Strain A/PR/8/34 Challenge in Mice Immunized with Non-Adjuvanted Novirhabdovirus Vaccines</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-10-06</date><risdate>2016</risdate><volume>11</volume><issue>10</issue><spage>e0164245</spage><epage>e0164245</epage><pages>e0164245-e0164245</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Novirhabdoviruses like Viral Hemorrhagic Septicemia Virus (VHSV) and Infectious Hematopoietic Necrosis Virus (IHNV) are fish-infecting Rhabdoviruses belonging to the Mononegavirales order. By reverse genetics, we previously showed that a recombinant VHSV expressing the West Nile Virus (WNV) E glycoprotein could serve as a vaccine platform against WNV. In the current study, we aimed to evaluate the potential of the Novirhabdovirus platform as a vaccine against influenza virus. Recombinant Novirhabdoviruses, rVHSV-HA and rIHNV-HA, expressing at the viral surface the hemagglutinin HA ectodomain were generated and used to immunized mice. We showed that mice immunized with either, rVHSV-HA or rIHNV-HA, elicited a strong neutralizing antibody response against influenza virus. A complete protection was conferred to the immunized mice when challenged with a lethal dose of influenza H1N1 A/PR/8/34 virus. Furthermore we showed that although acting as inert antigen in mice, since naturally inactivated over 20°C, mice immunized with rVHSV-HA or rIHNV-HA in the absence of adjuvant were also completely protected from a lethal challenge. Novirhabdoviruses platform are of particular interest as vaccines for mammals since they are cost effective to produce, relatively easy to generate and very effective to protect immunized animals.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27711176</pmid><doi>10.1371/journal.pone.0164245</doi><tpages>e0164245</tpages><orcidid>https://orcid.org/0000-0002-7020-2391</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1826490511 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies, Neutralizing - blood Antibodies, Neutralizing - immunology Antibody response Antigens Biology and life sciences Carps Cell Line Dogs Female Fluorescent Antibody Technique, Indirect Genetics Genomes Glycoproteins Hemagglutinins Hemagglutinins - genetics Hemagglutinins - immunology Hemagglutinins - metabolism Hemorrhage Hemorrhagic septicemia Immunization Infections Infectious hematopoietic necrosis Influenza Influenza A Virus, H1N1 Subtype - immunology Influenza A Virus, H1N1 Subtype - pathogenicity Influenza vaccines Influenza viruses Lectins Lethal dose Life Sciences Madin Darby Canine Kidney Cells Medical research Medicine and Health Sciences Mice Mice, Inbred BALB C Microscopy, Electron Mutagenesis Novirhabdovirus - immunology Novirhabdovirus - metabolism Orthomyxoviridae Infections - immunology Orthomyxoviridae Infections - prevention & control Pandemics Plasmids - genetics Plasmids - metabolism Production capacity Proteins Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - immunology Research and Analysis Methods Septicemia Swine flu Vaccination Vaccines Vaccines, Synthetic - immunology Viruses West Nile fever West Nile virus Wildlife conservation |
| title | Complete Protection against Influenza Virus H1N1 Strain A/PR/8/34 Challenge in Mice Immunized with Non-Adjuvanted Novirhabdovirus Vaccines |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T03%3A16%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Complete%20Protection%20against%20Influenza%20Virus%20H1N1%20Strain%20A/PR/8/34%20Challenge%20in%20Mice%20Immunized%20with%20Non-Adjuvanted%20Novirhabdovirus%20Vaccines&rft.jtitle=PloS%20one&rft.au=Rouxel,%20Ronan%20N&rft.date=2016-10-06&rft.volume=11&rft.issue=10&rft.spage=e0164245&rft.epage=e0164245&rft.pages=e0164245-e0164245&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0164245&rft_dat=%3Cgale_plos_%3EA471829428%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1826490511&rft_id=info:pmid/27711176&rft_galeid=A471829428&rft_doaj_id=oai_doaj_org_article_7d0a2adf5c8c44b7a2c8d0b8c1daa58e&rfr_iscdi=true |