Microbiome in the Apical Root Canal System of Teeth with Post-Treatment Apical Periodontitis
Bacteria present in the apical root canal system are directly involved with the pathogenesis of post-treatment apical periodontitis. This study used a next-generation sequencing approach to identify the bacterial taxa occurring in cryopulverized apical root samples from root canal-treated teeth with...
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| Veröffentlicht in: | PloS one 2016-09, Vol.11 (9), p.e0162887-e0162887 |
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| creator | Siqueira, Jr, José F Antunes, Henrique S Rôças, Isabela N Rachid, Caio T C C Alves, Flávio R F |
| description | Bacteria present in the apical root canal system are directly involved with the pathogenesis of post-treatment apical periodontitis. This study used a next-generation sequencing approach to identify the bacterial taxa occurring in cryopulverized apical root samples from root canal-treated teeth with post-treatment disease.
Apical root specimens obtained during periradicular surgery of ten adequately treated teeth with persistent apical periodontitis were cryogenically ground. DNA was extracted from the powder and the microbiome was characterized on the basis of the V4 hypervariable region of the 16S rRNA gene by using paired-end sequencing on Illumina MiSeq device.
All samples were positive for the presence of bacterial DNA. Bacterial taxa were mapped to 11 phyla and 103 genera composed by 538 distinct operational taxonomic units (OTUs) at 3% of dissimilarity. Over 85% of the sequences belonged to 4 phyla: Proteobacteria, Firmicutes, Fusobacteria and Actinobacteria. In general, these 4 phyla accounted for approximately 80% of the distinct OTUs found in the apical root samples. Proteobacteria was the most abundant phylum in 6/10 samples. Fourteen genera had representatives identified in all cases. Overall, the genera Fusobacterium and Pseudomonas were the most dominant. Enterococcus was found in 4 cases, always in relatively low abundance.
This study showed a highly complex bacterial community in the apical root canal system of adequately treated teeth with persistent apical periodontitis. This suggests that this disease is characterized by multispecies bacterial communities and has a heterogeneous etiology, because the community composition largely varied from case to case. |
| doi_str_mv | 10.1371/journal.pone.0162887 |
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Apical root specimens obtained during periradicular surgery of ten adequately treated teeth with persistent apical periodontitis were cryogenically ground. DNA was extracted from the powder and the microbiome was characterized on the basis of the V4 hypervariable region of the 16S rRNA gene by using paired-end sequencing on Illumina MiSeq device.
All samples were positive for the presence of bacterial DNA. Bacterial taxa were mapped to 11 phyla and 103 genera composed by 538 distinct operational taxonomic units (OTUs) at 3% of dissimilarity. Over 85% of the sequences belonged to 4 phyla: Proteobacteria, Firmicutes, Fusobacteria and Actinobacteria. In general, these 4 phyla accounted for approximately 80% of the distinct OTUs found in the apical root samples. Proteobacteria was the most abundant phylum in 6/10 samples. Fourteen genera had representatives identified in all cases. Overall, the genera Fusobacterium and Pseudomonas were the most dominant. Enterococcus was found in 4 cases, always in relatively low abundance.
This study showed a highly complex bacterial community in the apical root canal system of adequately treated teeth with persistent apical periodontitis. This suggests that this disease is characterized by multispecies bacterial communities and has a heterogeneous etiology, because the community composition largely varied from case to case.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0162887</identifier><identifier>PMID: 27689802</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Bacteria ; Bacterial infections ; Bacteroidetes ; Biology and Life Sciences ; Communities ; Community ; Community composition ; Dentistry ; Deoxyribonucleic acid ; Development and progression ; DNA ; Endodontics ; Engineering and Technology ; Etiology ; Firmicutes ; Gene sequencing ; Genera ; Gum disease ; Infections ; Laboratories ; Medical treatment ; Medicine and Health Sciences ; Microbiology ; Microbiomes ; Microbiota ; Pathogenesis ; Pathogens ; Periodontitis ; Powder ; Research and Analysis Methods ; RNA ; Root canal therapy ; Root canals ; rRNA 16S ; Studies ; Surgery ; Taxa ; Teeth</subject><ispartof>PloS one, 2016-09, Vol.11 (9), p.e0162887-e0162887</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Siqueira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Siqueira et al 2016 Siqueira et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-b6159178c280680b28f2e5ea69b71b22e2b61cfd65e118dec4d2f6a645398ee43</citedby><cites>FETCH-LOGICAL-c692t-b6159178c280680b28f2e5ea69b71b22e2b61cfd65e118dec4d2f6a645398ee43</cites><orcidid>0000-0002-9922-8202</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045198/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045198/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27689802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Siqueira, Jr, José F</creatorcontrib><creatorcontrib>Antunes, Henrique S</creatorcontrib><creatorcontrib>Rôças, Isabela N</creatorcontrib><creatorcontrib>Rachid, Caio T C C</creatorcontrib><creatorcontrib>Alves, Flávio R F</creatorcontrib><title>Microbiome in the Apical Root Canal System of Teeth with Post-Treatment Apical Periodontitis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bacteria present in the apical root canal system are directly involved with the pathogenesis of post-treatment apical periodontitis. This study used a next-generation sequencing approach to identify the bacterial taxa occurring in cryopulverized apical root samples from root canal-treated teeth with post-treatment disease.
Apical root specimens obtained during periradicular surgery of ten adequately treated teeth with persistent apical periodontitis were cryogenically ground. DNA was extracted from the powder and the microbiome was characterized on the basis of the V4 hypervariable region of the 16S rRNA gene by using paired-end sequencing on Illumina MiSeq device.
All samples were positive for the presence of bacterial DNA. Bacterial taxa were mapped to 11 phyla and 103 genera composed by 538 distinct operational taxonomic units (OTUs) at 3% of dissimilarity. Over 85% of the sequences belonged to 4 phyla: Proteobacteria, Firmicutes, Fusobacteria and Actinobacteria. In general, these 4 phyla accounted for approximately 80% of the distinct OTUs found in the apical root samples. Proteobacteria was the most abundant phylum in 6/10 samples. Fourteen genera had representatives identified in all cases. Overall, the genera Fusobacterium and Pseudomonas were the most dominant. Enterococcus was found in 4 cases, always in relatively low abundance.
This study showed a highly complex bacterial community in the apical root canal system of adequately treated teeth with persistent apical periodontitis. This suggests that this disease is characterized by multispecies bacterial communities and has a heterogeneous etiology, because the community composition largely varied from case to case.</description><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Bacteroidetes</subject><subject>Biology and Life Sciences</subject><subject>Communities</subject><subject>Community</subject><subject>Community composition</subject><subject>Dentistry</subject><subject>Deoxyribonucleic acid</subject><subject>Development and progression</subject><subject>DNA</subject><subject>Endodontics</subject><subject>Engineering and Technology</subject><subject>Etiology</subject><subject>Firmicutes</subject><subject>Gene sequencing</subject><subject>Genera</subject><subject>Gum disease</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Medical treatment</subject><subject>Medicine and Health 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One</addtitle><date>2016-09-30</date><risdate>2016</risdate><volume>11</volume><issue>9</issue><spage>e0162887</spage><epage>e0162887</epage><pages>e0162887-e0162887</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bacteria present in the apical root canal system are directly involved with the pathogenesis of post-treatment apical periodontitis. This study used a next-generation sequencing approach to identify the bacterial taxa occurring in cryopulverized apical root samples from root canal-treated teeth with post-treatment disease.
Apical root specimens obtained during periradicular surgery of ten adequately treated teeth with persistent apical periodontitis were cryogenically ground. DNA was extracted from the powder and the microbiome was characterized on the basis of the V4 hypervariable region of the 16S rRNA gene by using paired-end sequencing on Illumina MiSeq device.
All samples were positive for the presence of bacterial DNA. Bacterial taxa were mapped to 11 phyla and 103 genera composed by 538 distinct operational taxonomic units (OTUs) at 3% of dissimilarity. Over 85% of the sequences belonged to 4 phyla: Proteobacteria, Firmicutes, Fusobacteria and Actinobacteria. In general, these 4 phyla accounted for approximately 80% of the distinct OTUs found in the apical root samples. Proteobacteria was the most abundant phylum in 6/10 samples. Fourteen genera had representatives identified in all cases. Overall, the genera Fusobacterium and Pseudomonas were the most dominant. Enterococcus was found in 4 cases, always in relatively low abundance.
This study showed a highly complex bacterial community in the apical root canal system of adequately treated teeth with persistent apical periodontitis. This suggests that this disease is characterized by multispecies bacterial communities and has a heterogeneous etiology, because the community composition largely varied from case to case.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27689802</pmid><doi>10.1371/journal.pone.0162887</doi><tpages>e0162887</tpages><orcidid>https://orcid.org/0000-0002-9922-8202</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Bacteria Bacterial infections Bacteroidetes Biology and Life Sciences Communities Community Community composition Dentistry Deoxyribonucleic acid Development and progression DNA Endodontics Engineering and Technology Etiology Firmicutes Gene sequencing Genera Gum disease Infections Laboratories Medical treatment Medicine and Health Sciences Microbiology Microbiomes Microbiota Pathogenesis Pathogens Periodontitis Powder Research and Analysis Methods RNA Root canal therapy Root canals rRNA 16S Studies Surgery Taxa Teeth |
| title | Microbiome in the Apical Root Canal System of Teeth with Post-Treatment Apical Periodontitis |
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