Dietary Enrichment with 20% Fish Oil Decreases Mucus Production and the Inflammatory Response in Mice with Ovalbumin-Induced Allergic Lung Inflammation
The prevalence of asthma has increased in recent decades, which may be related to higher dietary intake of (n-6) polyunsaturated fatty acids (PUFA) and lower intake of (n-3) PUFA, e.g., those contained in fish oil. The objective of this study was to determine if dietary PUFA enrichment decreases muc...
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| Veröffentlicht in: | PloS one 2016-09, Vol.11 (9), p.e0163819-e0163819 |
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| creator | Hall, Jean A Hartman, Jaye Skinner, Monica M Schwindt, Adam R Fischer, Kay A Vorachek, William R Bobe, Gerd Valentine, Beth A |
| description | The prevalence of asthma has increased in recent decades, which may be related to higher dietary intake of (n-6) polyunsaturated fatty acids (PUFA) and lower intake of (n-3) PUFA, e.g., those contained in fish oil. The objective of this study was to determine if dietary PUFA enrichment decreases mucus production or the inflammatory response associated with ovalbumin (OVA)-induced allergic lung inflammation. Mice (n = 10/group) were fed control, 20% fish oil, or 20% corn oil enriched diets for a total of 12 weeks. At 8 and 10 weeks, mice were given an intraperitoneal injection of saline (10 control-fed mice) or OVA (30 remaining mice). Once at 10 weeks and on 3 consecutive days during week 12, mice were challenged by nebulizing with saline or OVA. Mice were euthanized 24 hours after the last challenge and blood was collected for plasma FA analysis. Bronchoalveolar lavage (BAL) fluid was collected to determine cell composition and Th2-type cytokine (IL-4, IL-13) concentrations. Periodic acid-Schiff (PAS) + mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue were quantified using morphometric analysis. Relative abundance of mRNA for mucin (Muc4, Muc5ac, and Muc5b) and Th2-type cytokine (IL-4, IL-5, and IL-13) genes were compared with ß-actin by qPCR. Supplementation with either corn oil or fish oil effectively altered plasma FA profiles towards more (n-6) FA or (n-3) FA, respectively (P < 0.0001). Sensitization and challenge with OVA increased the proportion of neutrophils, lymphocytes, and eosinophils, and decreased the proportion of macrophages and concentrations of IL-13 in BAL fluid; increased the percentage of PAS+ mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue; and increased gene expression of mucins (Muc4, Muc5ac, and Muc5b) and Th2-type cytokines (IL-5 and IL-13) in lung tissue of control-fed mice. Dietary PUFA reversed the increase in PAS+ mucus-producing cells (P = 0.003). In addition, dietary enrichment with fish oil attenuated the percentage of CD45+ inflammatory cell infiltrates in lung tissue, and increased Muc4 and Muc 5b gene expression compared with OVA-sensitized and challenged control mice. In conclusion, dietary enrichment with either (n-3) or (n-6) PUFA decreased mucus production in lung tissues of OVA-sensitized and challenged mice. More specifically, enrichment with dietary (n-3) PUFA decreased CD45+ inflammatory cell infiltrates, thus inducing potentially beneficial changes in lung |
| doi_str_mv | 10.1371/journal.pone.0163819 |
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The objective of this study was to determine if dietary PUFA enrichment decreases mucus production or the inflammatory response associated with ovalbumin (OVA)-induced allergic lung inflammation. Mice (n = 10/group) were fed control, 20% fish oil, or 20% corn oil enriched diets for a total of 12 weeks. At 8 and 10 weeks, mice were given an intraperitoneal injection of saline (10 control-fed mice) or OVA (30 remaining mice). Once at 10 weeks and on 3 consecutive days during week 12, mice were challenged by nebulizing with saline or OVA. Mice were euthanized 24 hours after the last challenge and blood was collected for plasma FA analysis. Bronchoalveolar lavage (BAL) fluid was collected to determine cell composition and Th2-type cytokine (IL-4, IL-13) concentrations. Periodic acid-Schiff (PAS) + mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue were quantified using morphometric analysis. Relative abundance of mRNA for mucin (Muc4, Muc5ac, and Muc5b) and Th2-type cytokine (IL-4, IL-5, and IL-13) genes were compared with ß-actin by qPCR. Supplementation with either corn oil or fish oil effectively altered plasma FA profiles towards more (n-6) FA or (n-3) FA, respectively (P < 0.0001). Sensitization and challenge with OVA increased the proportion of neutrophils, lymphocytes, and eosinophils, and decreased the proportion of macrophages and concentrations of IL-13 in BAL fluid; increased the percentage of PAS+ mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue; and increased gene expression of mucins (Muc4, Muc5ac, and Muc5b) and Th2-type cytokines (IL-5 and IL-13) in lung tissue of control-fed mice. Dietary PUFA reversed the increase in PAS+ mucus-producing cells (P = 0.003). In addition, dietary enrichment with fish oil attenuated the percentage of CD45+ inflammatory cell infiltrates in lung tissue, and increased Muc4 and Muc 5b gene expression compared with OVA-sensitized and challenged control mice. In conclusion, dietary enrichment with either (n-3) or (n-6) PUFA decreased mucus production in lung tissues of OVA-sensitized and challenged mice. More specifically, enrichment with dietary (n-3) PUFA decreased CD45+ inflammatory cell infiltrates, thus inducing potentially beneficial changes in lung tissue of OVA-sensitized and challenged mice.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0163819</identifier><identifier>PMID: 27669173</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actin ; Albumin ; Alveoli ; Animal tissues ; Asthma ; B cells ; Biology and Life Sciences ; Bronchoalveolar lavage ; Bronchus ; CD45 antigen ; Corn ; Corn oil ; Cytokines ; Diet ; Dietary intake ; Dietary supplements ; Drug dosages ; Enrichment ; Eosinophils ; Fatty acids ; Fish ; Fish oils ; Gene expression ; Inflammation ; Inflammatory response ; Interleukin 13 ; Interleukin 4 ; Interleukin 5 ; Leukocytes (eosinophilic) ; Leukocytes (neutrophilic) ; Lungs ; Lymphocytes ; Lymphocytes T ; Macrophages ; Medicine and Health Sciences ; Metabolism ; Metabolites ; Mice ; Morphometry ; Mucin ; Mucins ; Mucus ; Oils & fats ; Ovalbumin ; Polyunsaturated fatty acids ; Production management ; Relative abundance ; Rodents ; Statistics ; Supplementation ; Tissues ; Unsaturated fatty acids ; Vegetable oils ; Veterinary colleges ; Veterinary medicine</subject><ispartof>PloS one, 2016-09, Vol.11 (9), p.e0163819-e0163819</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Hall et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Hall et al 2016 Hall et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-95b7f46c970990a57a54545d5314725ca3fb9ca630791ede149fcc805d636c743</citedby><cites>FETCH-LOGICAL-c725t-95b7f46c970990a57a54545d5314725ca3fb9ca630791ede149fcc805d636c743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036796/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036796/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27669173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Aguila, Marcia B.</contributor><creatorcontrib>Hall, Jean A</creatorcontrib><creatorcontrib>Hartman, Jaye</creatorcontrib><creatorcontrib>Skinner, Monica M</creatorcontrib><creatorcontrib>Schwindt, Adam R</creatorcontrib><creatorcontrib>Fischer, Kay A</creatorcontrib><creatorcontrib>Vorachek, William R</creatorcontrib><creatorcontrib>Bobe, Gerd</creatorcontrib><creatorcontrib>Valentine, Beth A</creatorcontrib><title>Dietary Enrichment with 20% Fish Oil Decreases Mucus Production and the Inflammatory Response in Mice with Ovalbumin-Induced Allergic Lung Inflammation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The prevalence of asthma has increased in recent decades, which may be related to higher dietary intake of (n-6) polyunsaturated fatty acids (PUFA) and lower intake of (n-3) PUFA, e.g., those contained in fish oil. The objective of this study was to determine if dietary PUFA enrichment decreases mucus production or the inflammatory response associated with ovalbumin (OVA)-induced allergic lung inflammation. Mice (n = 10/group) were fed control, 20% fish oil, or 20% corn oil enriched diets for a total of 12 weeks. At 8 and 10 weeks, mice were given an intraperitoneal injection of saline (10 control-fed mice) or OVA (30 remaining mice). Once at 10 weeks and on 3 consecutive days during week 12, mice were challenged by nebulizing with saline or OVA. Mice were euthanized 24 hours after the last challenge and blood was collected for plasma FA analysis. Bronchoalveolar lavage (BAL) fluid was collected to determine cell composition and Th2-type cytokine (IL-4, IL-13) concentrations. Periodic acid-Schiff (PAS) + mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue were quantified using morphometric analysis. Relative abundance of mRNA for mucin (Muc4, Muc5ac, and Muc5b) and Th2-type cytokine (IL-4, IL-5, and IL-13) genes were compared with ß-actin by qPCR. Supplementation with either corn oil or fish oil effectively altered plasma FA profiles towards more (n-6) FA or (n-3) FA, respectively (P < 0.0001). Sensitization and challenge with OVA increased the proportion of neutrophils, lymphocytes, and eosinophils, and decreased the proportion of macrophages and concentrations of IL-13 in BAL fluid; increased the percentage of PAS+ mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue; and increased gene expression of mucins (Muc4, Muc5ac, and Muc5b) and Th2-type cytokines (IL-5 and IL-13) in lung tissue of control-fed mice. Dietary PUFA reversed the increase in PAS+ mucus-producing cells (P = 0.003). In addition, dietary enrichment with fish oil attenuated the percentage of CD45+ inflammatory cell infiltrates in lung tissue, and increased Muc4 and Muc 5b gene expression compared with OVA-sensitized and challenged control mice. In conclusion, dietary enrichment with either (n-3) or (n-6) PUFA decreased mucus production in lung tissues of OVA-sensitized and challenged mice. More specifically, enrichment with dietary (n-3) PUFA decreased CD45+ inflammatory cell infiltrates, thus inducing potentially beneficial changes in lung tissue of OVA-sensitized and challenged mice.</description><subject>Actin</subject><subject>Albumin</subject><subject>Alveoli</subject><subject>Animal tissues</subject><subject>Asthma</subject><subject>B cells</subject><subject>Biology and Life Sciences</subject><subject>Bronchoalveolar lavage</subject><subject>Bronchus</subject><subject>CD45 antigen</subject><subject>Corn</subject><subject>Corn oil</subject><subject>Cytokines</subject><subject>Diet</subject><subject>Dietary intake</subject><subject>Dietary supplements</subject><subject>Drug dosages</subject><subject>Enrichment</subject><subject>Eosinophils</subject><subject>Fatty acids</subject><subject>Fish</subject><subject>Fish oils</subject><subject>Gene expression</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 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Enrichment with 20% Fish Oil Decreases Mucus Production and the Inflammatory Response in Mice with Ovalbumin-Induced Allergic Lung Inflammation</title><author>Hall, Jean A ; Hartman, Jaye ; Skinner, Monica M ; Schwindt, Adam R ; Fischer, Kay A ; Vorachek, William R ; Bobe, Gerd ; Valentine, Beth A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-95b7f46c970990a57a54545d5314725ca3fb9ca630791ede149fcc805d636c743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Actin</topic><topic>Albumin</topic><topic>Alveoli</topic><topic>Animal tissues</topic><topic>Asthma</topic><topic>B cells</topic><topic>Biology and Life Sciences</topic><topic>Bronchoalveolar lavage</topic><topic>Bronchus</topic><topic>CD45 antigen</topic><topic>Corn</topic><topic>Corn oil</topic><topic>Cytokines</topic><topic>Diet</topic><topic>Dietary intake</topic><topic>Dietary supplements</topic><topic>Drug 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Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hall, Jean A</au><au>Hartman, Jaye</au><au>Skinner, Monica M</au><au>Schwindt, Adam R</au><au>Fischer, Kay A</au><au>Vorachek, William R</au><au>Bobe, Gerd</au><au>Valentine, Beth A</au><au>Aguila, Marcia B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary Enrichment with 20% Fish Oil Decreases Mucus Production and the Inflammatory Response in Mice with Ovalbumin-Induced Allergic Lung Inflammation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-09-26</date><risdate>2016</risdate><volume>11</volume><issue>9</issue><spage>e0163819</spage><epage>e0163819</epage><pages>e0163819-e0163819</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The prevalence of asthma has increased in recent decades, which may be related to higher dietary intake of (n-6) polyunsaturated fatty acids (PUFA) and lower intake of (n-3) PUFA, e.g., those contained in fish oil. The objective of this study was to determine if dietary PUFA enrichment decreases mucus production or the inflammatory response associated with ovalbumin (OVA)-induced allergic lung inflammation. Mice (n = 10/group) were fed control, 20% fish oil, or 20% corn oil enriched diets for a total of 12 weeks. At 8 and 10 weeks, mice were given an intraperitoneal injection of saline (10 control-fed mice) or OVA (30 remaining mice). Once at 10 weeks and on 3 consecutive days during week 12, mice were challenged by nebulizing with saline or OVA. Mice were euthanized 24 hours after the last challenge and blood was collected for plasma FA analysis. Bronchoalveolar lavage (BAL) fluid was collected to determine cell composition and Th2-type cytokine (IL-4, IL-13) concentrations. Periodic acid-Schiff (PAS) + mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue were quantified using morphometric analysis. Relative abundance of mRNA for mucin (Muc4, Muc5ac, and Muc5b) and Th2-type cytokine (IL-4, IL-5, and IL-13) genes were compared with ß-actin by qPCR. Supplementation with either corn oil or fish oil effectively altered plasma FA profiles towards more (n-6) FA or (n-3) FA, respectively (P < 0.0001). Sensitization and challenge with OVA increased the proportion of neutrophils, lymphocytes, and eosinophils, and decreased the proportion of macrophages and concentrations of IL-13 in BAL fluid; increased the percentage of PAS+ mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue; and increased gene expression of mucins (Muc4, Muc5ac, and Muc5b) and Th2-type cytokines (IL-5 and IL-13) in lung tissue of control-fed mice. Dietary PUFA reversed the increase in PAS+ mucus-producing cells (P = 0.003). In addition, dietary enrichment with fish oil attenuated the percentage of CD45+ inflammatory cell infiltrates in lung tissue, and increased Muc4 and Muc 5b gene expression compared with OVA-sensitized and challenged control mice. In conclusion, dietary enrichment with either (n-3) or (n-6) PUFA decreased mucus production in lung tissues of OVA-sensitized and challenged mice. More specifically, enrichment with dietary (n-3) PUFA decreased CD45+ inflammatory cell infiltrates, thus inducing potentially beneficial changes in lung tissue of OVA-sensitized and challenged mice.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27669173</pmid><doi>10.1371/journal.pone.0163819</doi><tpages>e0163819</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-09, Vol.11 (9), p.e0163819-e0163819 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1823427496 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Actin Albumin Alveoli Animal tissues Asthma B cells Biology and Life Sciences Bronchoalveolar lavage Bronchus CD45 antigen Corn Corn oil Cytokines Diet Dietary intake Dietary supplements Drug dosages Enrichment Eosinophils Fatty acids Fish Fish oils Gene expression Inflammation Inflammatory response Interleukin 13 Interleukin 4 Interleukin 5 Leukocytes (eosinophilic) Leukocytes (neutrophilic) Lungs Lymphocytes Lymphocytes T Macrophages Medicine and Health Sciences Metabolism Metabolites Mice Morphometry Mucin Mucins Mucus Oils & fats Ovalbumin Polyunsaturated fatty acids Production management Relative abundance Rodents Statistics Supplementation Tissues Unsaturated fatty acids Vegetable oils Veterinary colleges Veterinary medicine |
| title | Dietary Enrichment with 20% Fish Oil Decreases Mucus Production and the Inflammatory Response in Mice with Ovalbumin-Induced Allergic Lung Inflammation |
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