Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli
The filamentous fungi Phaeoacremonium aleophilum (P.al, Teleomorph: Togninia minima) and Phaeomoniella chlamydospora (P.ch) are believed to be causal agents of wood symptoms associated with the Esca associated young vine decline. The occurrence of these diseases is dramatically increasing in vineyar...
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| description | The filamentous fungi Phaeoacremonium aleophilum (P.al, Teleomorph: Togninia minima) and Phaeomoniella chlamydospora (P.ch) are believed to be causal agents of wood symptoms associated with the Esca associated young vine decline. The occurrence of these diseases is dramatically increasing in vineyards all over the world whereas efficient therapeutic strategies are lacking. Both fungi occupy the same ecological niche within the grapevine trunk. We found them predominantly within the xylem vessels and surrounding cell walls which raises the question whether the transcriptional response towards plant cell secreted metabolites is comparable. In order to address this question we co-inoculated grapevine callus culture cells with the respective fungi and analyzed their transcriptomes by RNA sequencing. This experimental setup appears suitable since we aimed to investigate the effects caused by the plant thereby excluding all effects caused by other microorganisms omnipresent in planta and nutrient depletion. Bioinformatics analysis of the sequencing data revealed that 837 homologous genes were found to have comparable expression pattern whereas none of which was found to be differentially expressed in both strains upon exposure to the plant cells. Despite the fact that both fungi induced the transcription of oxido- reductases, likely to cope with reactive oxygen species produced by plant cells, the transcriptomics response of both fungi compared to each other is rather different in other domains. Within the transcriptome of P.ch beside increased transcript levels for oxido- reductases, plant cell wall degrading enzymes and detoxifying enzymes were found. On the other hand in P.al the transcription of some oxido- reductases was increased whereas others appeared to be repressed. In this fungus the confrontation to plant cells results in higher transcript levels of heat shock and chaperon-like proteins as well as genes encoding proteins involved in primary metabolism. |
| doi_str_mv | 10.1371/journal.pone.0163344 |
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The occurrence of these diseases is dramatically increasing in vineyards all over the world whereas efficient therapeutic strategies are lacking. Both fungi occupy the same ecological niche within the grapevine trunk. We found them predominantly within the xylem vessels and surrounding cell walls which raises the question whether the transcriptional response towards plant cell secreted metabolites is comparable. In order to address this question we co-inoculated grapevine callus culture cells with the respective fungi and analyzed their transcriptomes by RNA sequencing. This experimental setup appears suitable since we aimed to investigate the effects caused by the plant thereby excluding all effects caused by other microorganisms omnipresent in planta and nutrient depletion. Bioinformatics analysis of the sequencing data revealed that 837 homologous genes were found to have comparable expression pattern whereas none of which was found to be differentially expressed in both strains upon exposure to the plant cells. Despite the fact that both fungi induced the transcription of oxido- reductases, likely to cope with reactive oxygen species produced by plant cells, the transcriptomics response of both fungi compared to each other is rather different in other domains. Within the transcriptome of P.ch beside increased transcript levels for oxido- reductases, plant cell wall degrading enzymes and detoxifying enzymes were found. On the other hand in P.al the transcription of some oxido- reductases was increased whereas others appeared to be repressed. In this fungus the confrontation to plant cells results in higher transcript levels of heat shock and chaperon-like proteins as well as genes encoding proteins involved in primary metabolism.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0163344</identifier><identifier>PMID: 27658052</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Bioinformatics ; Biology and Life Sciences ; Callus ; Callus culture ; Cell culture ; Cell walls ; Disease ; Ecological niches ; Enzymes ; Flavonoids ; Fungi ; Gene expression ; Gene sequencing ; Genes ; Genomes ; Grapes ; Health aspects ; Heat shock ; Homology ; Kinases ; Laboratories ; Medicine and Health Sciences ; Metabolism ; Metabolites ; Microorganisms ; Niches (Ecology) ; Oxygen ; Pathogens ; Perfect state ; Phaeoacremonium ; Phaeomoniella chlamydospora ; Physiological aspects ; Plant cells ; Proteins ; Reactive oxygen species ; Reductases ; Ribonucleic acid ; RNA ; RNA sequencing ; Transcription ; Vineyards ; Vitaceae ; Vitis vinifera ; Wineries ; Wood ; Xylem</subject><ispartof>PloS one, 2016-09, Vol.11 (9), p.e0163344-e0163344</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Fischer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Fischer et al 2016 Fischer et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-dd3620e363e16831c13b3cda332551f1f881482945f723ad1859f311e0fafe443</citedby><cites>FETCH-LOGICAL-c725t-dd3620e363e16831c13b3cda332551f1f881482945f723ad1859f311e0fafe443</cites><orcidid>0000-0002-0413-7763</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033417/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033417/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27658052$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gerós, Hernâni</contributor><creatorcontrib>Fischer, Jochen</creatorcontrib><creatorcontrib>Compant, Stéphane</creatorcontrib><creatorcontrib>Pierron, Romain J G</creatorcontrib><creatorcontrib>Gorfer, Markus</creatorcontrib><creatorcontrib>Jacques, Alban</creatorcontrib><creatorcontrib>Thines, Eckhard</creatorcontrib><creatorcontrib>Berger, Harald</creatorcontrib><title>Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The filamentous fungi Phaeoacremonium aleophilum (P.al, Teleomorph: Togninia minima) and Phaeomoniella chlamydospora (P.ch) are believed to be causal agents of wood symptoms associated with the Esca associated young vine decline. The occurrence of these diseases is dramatically increasing in vineyards all over the world whereas efficient therapeutic strategies are lacking. Both fungi occupy the same ecological niche within the grapevine trunk. We found them predominantly within the xylem vessels and surrounding cell walls which raises the question whether the transcriptional response towards plant cell secreted metabolites is comparable. In order to address this question we co-inoculated grapevine callus culture cells with the respective fungi and analyzed their transcriptomes by RNA sequencing. This experimental setup appears suitable since we aimed to investigate the effects caused by the plant thereby excluding all effects caused by other microorganisms omnipresent in planta and nutrient depletion. Bioinformatics analysis of the sequencing data revealed that 837 homologous genes were found to have comparable expression pattern whereas none of which was found to be differentially expressed in both strains upon exposure to the plant cells. Despite the fact that both fungi induced the transcription of oxido- reductases, likely to cope with reactive oxygen species produced by plant cells, the transcriptomics response of both fungi compared to each other is rather different in other domains. Within the transcriptome of P.ch beside increased transcript levels for oxido- reductases, plant cell wall degrading enzymes and detoxifying enzymes were found. On the other hand in P.al the transcription of some oxido- reductases was increased whereas others appeared to be repressed. In this fungus the confrontation to plant cells results in higher transcript levels of heat shock and chaperon-like proteins as well as genes encoding proteins involved in primary metabolism.</description><subject>Analysis</subject><subject>Bioinformatics</subject><subject>Biology and Life Sciences</subject><subject>Callus</subject><subject>Callus culture</subject><subject>Cell culture</subject><subject>Cell walls</subject><subject>Disease</subject><subject>Ecological niches</subject><subject>Enzymes</subject><subject>Flavonoids</subject><subject>Fungi</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Grapes</subject><subject>Health aspects</subject><subject>Heat shock</subject><subject>Homology</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Medicine and Health 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Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli</title><author>Fischer, Jochen ; Compant, Stéphane ; Pierron, Romain J G ; Gorfer, Markus ; Jacques, Alban ; Thines, Eckhard ; Berger, Harald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-dd3620e363e16831c13b3cda332551f1f881482945f723ad1859f311e0fafe443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Bioinformatics</topic><topic>Biology and Life Sciences</topic><topic>Callus</topic><topic>Callus culture</topic><topic>Cell culture</topic><topic>Cell walls</topic><topic>Disease</topic><topic>Ecological niches</topic><topic>Enzymes</topic><topic>Flavonoids</topic><topic>Fungi</topic><topic>Gene expression</topic><topic>Gene 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Stéphane</au><au>Pierron, Romain J G</au><au>Gorfer, Markus</au><au>Jacques, Alban</au><au>Thines, Eckhard</au><au>Berger, Harald</au><au>Gerós, Hernâni</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-09-22</date><risdate>2016</risdate><volume>11</volume><issue>9</issue><spage>e0163344</spage><epage>e0163344</epage><pages>e0163344-e0163344</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The filamentous fungi Phaeoacremonium aleophilum (P.al, Teleomorph: Togninia minima) and Phaeomoniella chlamydospora (P.ch) are believed to be causal agents of wood symptoms associated with the Esca associated young vine decline. The occurrence of these diseases is dramatically increasing in vineyards all over the world whereas efficient therapeutic strategies are lacking. Both fungi occupy the same ecological niche within the grapevine trunk. We found them predominantly within the xylem vessels and surrounding cell walls which raises the question whether the transcriptional response towards plant cell secreted metabolites is comparable. In order to address this question we co-inoculated grapevine callus culture cells with the respective fungi and analyzed their transcriptomes by RNA sequencing. This experimental setup appears suitable since we aimed to investigate the effects caused by the plant thereby excluding all effects caused by other microorganisms omnipresent in planta and nutrient depletion. Bioinformatics analysis of the sequencing data revealed that 837 homologous genes were found to have comparable expression pattern whereas none of which was found to be differentially expressed in both strains upon exposure to the plant cells. Despite the fact that both fungi induced the transcription of oxido- reductases, likely to cope with reactive oxygen species produced by plant cells, the transcriptomics response of both fungi compared to each other is rather different in other domains. Within the transcriptome of P.ch beside increased transcript levels for oxido- reductases, plant cell wall degrading enzymes and detoxifying enzymes were found. On the other hand in P.al the transcription of some oxido- reductases was increased whereas others appeared to be repressed. In this fungus the confrontation to plant cells results in higher transcript levels of heat shock and chaperon-like proteins as well as genes encoding proteins involved in primary metabolism.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27658052</pmid><doi>10.1371/journal.pone.0163344</doi><tpages>e0163344</tpages><orcidid>https://orcid.org/0000-0002-0413-7763</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2016-09, Vol.11 (9), p.e0163344-e0163344 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1822391599 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Bioinformatics Biology and Life Sciences Callus Callus culture Cell culture Cell walls Disease Ecological niches Enzymes Flavonoids Fungi Gene expression Gene sequencing Genes Genomes Grapes Health aspects Heat shock Homology Kinases Laboratories Medicine and Health Sciences Metabolism Metabolites Microorganisms Niches (Ecology) Oxygen Pathogens Perfect state Phaeoacremonium Phaeomoniella chlamydospora Physiological aspects Plant cells Proteins Reactive oxygen species Reductases Ribonucleic acid RNA RNA sequencing Transcription Vineyards Vitaceae Vitis vinifera Wineries Wood Xylem |
| title | Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T13%3A16%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differing%20Alterations%20of%20Two%20Esca%20Associated%20Fungi,%20Phaeoacremonium%20aleophilum%20and%20Phaeomoniella%20chlamydospora%20on%20Transcriptomic%20Level,%20to%20Co-Cultured%20Vitis%20vinifera%20L.%20calli&rft.jtitle=PloS%20one&rft.au=Fischer,%20Jochen&rft.date=2016-09-22&rft.volume=11&rft.issue=9&rft.spage=e0163344&rft.epage=e0163344&rft.pages=e0163344-e0163344&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0163344&rft_dat=%3Cgale_plos_%3EA471899300%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1822391599&rft_id=info:pmid/27658052&rft_galeid=A471899300&rft_doaj_id=oai_doaj_org_article_967fdad1cebe4183a4d93d09dfff7916&rfr_iscdi=true |