A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies

Staphylococcal protein A (SpA) and streptococcal protein G (SpG) affinity chromatography are the gold standards for purifying monoclonal antibodies (mAbs) in therapeutic applications. However, camelid VHH single-domain Abs (sdAbs or VHHs) are not bound by SpG and only sporadically bound by SpA. Curr...

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Veröffentlicht in:	PloS one 2016-09, Vol.11 (9), p.e0163113
Hauptverfasser:	Henry, Kevin A, Sulea, Traian, van Faassen, Henk, Hussack, Greg, Purisima, Enrico O, MacKenzie, C Roger, Arbabi-Ghahroudi, Mehdi
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Affinity chromatography Animals Antigens Binding Bioinformatics Biology and Life Sciences Camelids, New World - immunology Cancer Chromatography Councils Environmental science Frequency variation Genes Health aspects Immunoglobulins Immunological tolerance Medicine and Health Sciences Monoclonal antibodies Mutagenesis Mutation Nanobodies Physical Sciences Protein A Protein binding Protein Engineering Protein G Proteins Purification Research and analysis methods Single-Domain Antibodies - genetics Staphylococcal Protein A - genetics Streptococcal protein G Streptococcus Therapeutic applications
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