Evaluation of Genotoxic Pressure along the Sava River
In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The geno...
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| Veröffentlicht in: | PloS one 2016-09, Vol.11 (9), p.e0162450 |
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| creator | Kolarević, Stoimir Aborgiba, Mustafa Kračun-Kolarević, Margareta Kostić, Jovana Simonović, Predrag Simić, Vladica Milošković, Aleksandra Reischer, Georg Farnleitner, Andreas Gačić, Zoran Milačič, Radmila Zuliani, Tea Vidmar, Janja Pergal, Marija Piria, Marina Paunović, Momir Vuković-Gačić, Branka |
| description | In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples). |
| doi_str_mv | 10.1371/journal.pone.0162450 |
| format | Article |
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In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0162450</identifier><identifier>PMID: 27631093</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alburnoides bipunctatus ; Alburnus alburnus ; Analysis ; Animals ; Aquatic ecosystems ; Batteries ; Bioassays ; Biodiversity ; Bioindicators ; Biology ; Biology and life sciences ; Biomarkers ; Blood cells ; Chemical engineering ; Comet assay ; Cooperation ; Damage detection ; Deoxyribonucleic acid ; DNA ; DNA damage ; Earth Sciences ; Ecology and Environmental Sciences ; Ecosystem biology ; Engineering and Technology ; Environmental science ; Eukaryotes ; Europe ; Evaluation ; Fishes ; Freshwater ; Freshwater fish ; Genotoxicity ; Homogeneity ; Mollusks ; Mutagenesis ; Mutagenicity ; Mutagenicity Tests ; Mutagens - toxicity ; Oxidative stress ; Pressure ; Prokaryotes ; Research and Analysis Methods ; Rivers ; Salmonella ; Salmonella Typhimurium ; Salmonella typhimurium - drug effects ; Salmonella typhimurium - genetics ; Sediments ; SOS Response (Genetics) ; Studies ; Toxicity ; Wastewater ; Water Pollutants, Chemical - toxicity ; Water quality</subject><ispartof>PloS one, 2016-09, Vol.11 (9), p.e0162450</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Kolarević et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Kolarević et al 2016 Kolarević et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-c924977010240b9a3b120f8ab9d39beb4c3cd1c2451762edcc9c619b3c7be5163</citedby><cites>FETCH-LOGICAL-c725t-c924977010240b9a3b120f8ab9d39beb4c3cd1c2451762edcc9c619b3c7be5163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025182/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025182/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27631093$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>LI, Mei</contributor><creatorcontrib>Kolarević, Stoimir</creatorcontrib><creatorcontrib>Aborgiba, Mustafa</creatorcontrib><creatorcontrib>Kračun-Kolarević, Margareta</creatorcontrib><creatorcontrib>Kostić, Jovana</creatorcontrib><creatorcontrib>Simonović, Predrag</creatorcontrib><creatorcontrib>Simić, Vladica</creatorcontrib><creatorcontrib>Milošković, Aleksandra</creatorcontrib><creatorcontrib>Reischer, Georg</creatorcontrib><creatorcontrib>Farnleitner, Andreas</creatorcontrib><creatorcontrib>Gačić, Zoran</creatorcontrib><creatorcontrib>Milačič, Radmila</creatorcontrib><creatorcontrib>Zuliani, Tea</creatorcontrib><creatorcontrib>Vidmar, Janja</creatorcontrib><creatorcontrib>Pergal, Marija</creatorcontrib><creatorcontrib>Piria, Marina</creatorcontrib><creatorcontrib>Paunović, Momir</creatorcontrib><creatorcontrib>Vuković-Gačić, Branka</creatorcontrib><title>Evaluation of Genotoxic Pressure along the Sava River</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).</description><subject>Alburnoides bipunctatus</subject><subject>Alburnus alburnus</subject><subject>Analysis</subject><subject>Animals</subject><subject>Aquatic ecosystems</subject><subject>Batteries</subject><subject>Bioassays</subject><subject>Biodiversity</subject><subject>Bioindicators</subject><subject>Biology</subject><subject>Biology and life sciences</subject><subject>Biomarkers</subject><subject>Blood cells</subject><subject>Chemical engineering</subject><subject>Comet assay</subject><subject>Cooperation</subject><subject>Damage detection</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Ecosystem biology</subject><subject>Engineering and Technology</subject><subject>Environmental science</subject><subject>Eukaryotes</subject><subject>Europe</subject><subject>Evaluation</subject><subject>Fishes</subject><subject>Freshwater</subject><subject>Freshwater fish</subject><subject>Genotoxicity</subject><subject>Homogeneity</subject><subject>Mollusks</subject><subject>Mutagenesis</subject><subject>Mutagenicity</subject><subject>Mutagenicity Tests</subject><subject>Mutagens - toxicity</subject><subject>Oxidative stress</subject><subject>Pressure</subject><subject>Prokaryotes</subject><subject>Research and Analysis Methods</subject><subject>Rivers</subject><subject>Salmonella</subject><subject>Salmonella Typhimurium</subject><subject>Salmonella typhimurium - drug effects</subject><subject>Salmonella typhimurium - genetics</subject><subject>Sediments</subject><subject>SOS Response (Genetics)</subject><subject>Studies</subject><subject>Toxicity</subject><subject>Wastewater</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>Water 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Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kolarević, Stoimir</au><au>Aborgiba, Mustafa</au><au>Kračun-Kolarević, Margareta</au><au>Kostić, Jovana</au><au>Simonović, Predrag</au><au>Simić, Vladica</au><au>Milošković, Aleksandra</au><au>Reischer, Georg</au><au>Farnleitner, Andreas</au><au>Gačić, Zoran</au><au>Milačič, Radmila</au><au>Zuliani, Tea</au><au>Vidmar, Janja</au><au>Pergal, Marija</au><au>Piria, Marina</au><au>Paunović, Momir</au><au>Vuković-Gačić, Branka</au><au>LI, Mei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Genotoxic Pressure along the Sava River</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-09-15</date><risdate>2016</risdate><volume>11</volume><issue>9</issue><spage>e0162450</spage><pages>e0162450-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27631093</pmid><doi>10.1371/journal.pone.0162450</doi><tpages>e0162450</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-09, Vol.11 (9), p.e0162450 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1819907339 |
| source | MEDLINE; Public Library of Science; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Alburnoides bipunctatus Alburnus alburnus Analysis Animals Aquatic ecosystems Batteries Bioassays Biodiversity Bioindicators Biology Biology and life sciences Biomarkers Blood cells Chemical engineering Comet assay Cooperation Damage detection Deoxyribonucleic acid DNA DNA damage Earth Sciences Ecology and Environmental Sciences Ecosystem biology Engineering and Technology Environmental science Eukaryotes Europe Evaluation Fishes Freshwater Freshwater fish Genotoxicity Homogeneity Mollusks Mutagenesis Mutagenicity Mutagenicity Tests Mutagens - toxicity Oxidative stress Pressure Prokaryotes Research and Analysis Methods Rivers Salmonella Salmonella Typhimurium Salmonella typhimurium - drug effects Salmonella typhimurium - genetics Sediments SOS Response (Genetics) Studies Toxicity Wastewater Water Pollutants, Chemical - toxicity Water quality |
| title | Evaluation of Genotoxic Pressure along the Sava River |
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