Treatment of Oral Candidiasis Using Photodithazine®- Mediated Photodynamic Therapy In Vivo
This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine® (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. S...
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| description | This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine® (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (p |
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This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (p<0.05). Animals underwent PDZ-mediated aPDT showed complete remission of oral lesions, while animals treated with NYS presented partial remission of oral lesions in both periods assessed. Histological evaluation revealed mild inflammatory infiltrate in the groups treated with aPDT and NYS in both periods assessed. The aPDT induced the TNF-α expression when compared with the control (P-L-) (p<0.05), 24 h and 7 days after treatment. In summary, the murine model developed here was able to mimic the infection and PDZ-mediated aPDT was effective to treat mice with oral candidiasis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0156947</identifier><identifier>PMID: 27253525</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal models ; Animals ; Biofilms ; Biology and Life Sciences ; Cancer ; Candida ; Candida albicans ; Candida albicans - drug effects ; Candida albicans - growth & development ; Candidiasis ; Candidiasis, Oral - drug therapy ; Candidiasis, Oral - genetics ; Candidiasis, Oral - microbiology ; Candidiasis, Oral - pathology ; Colony Count, Microbial ; Cytochrome ; Cytokines ; Data processing ; Deactivation ; Dental schools ; Diabetes ; Disease Models, Animal ; Female ; Gene expression ; Gene Expression Regulation - drug effects ; Glucosamine - analogs & derivatives ; Glucosamine - pharmacology ; Glucosamine - therapeutic use ; In vivo methods and tests ; Inactivation ; Infections ; Inflammation ; Interleukin 1 ; Interleukin 6 ; Interleukin-1beta - genetics ; Interleukin-1beta - metabolism ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Kinetics ; Lasers ; Lesions ; Light ; Medicine and Health Sciences ; Mice ; Microorganisms ; Nystatin ; Performance evaluation ; Photochemotherapy ; Photodynamic therapy ; Physiology ; Remission ; Research and Analysis Methods ; Studies ; Tongue ; Treatment Outcome ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Variance analysis</subject><ispartof>PloS one, 2016-06, Vol.11 (6), p.e0156947</ispartof><rights>2016 Carmello et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Carmello et al 2016 Carmello et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-852a3781970fbe1aa61175ab8c5543e4438fa9db990b30c46e04d1d19323ad453</citedby><cites>FETCH-LOGICAL-c592t-852a3781970fbe1aa61175ab8c5543e4438fa9db990b30c46e04d1d19323ad453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890797/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890797/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27253525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carmello, Juliana Cabrini</creatorcontrib><creatorcontrib>Alves, Fernanda</creatorcontrib><creatorcontrib>G Basso, Fernanda</creatorcontrib><creatorcontrib>de Souza Costa, Carlos Alberto</creatorcontrib><creatorcontrib>Bagnato, Vanderlei Salvador</creatorcontrib><creatorcontrib>Mima, Ewerton Garcia de Oliveira</creatorcontrib><creatorcontrib>Pavarina, Ana Cláudia</creatorcontrib><title>Treatment of Oral Candidiasis Using Photodithazine®- Mediated Photodynamic Therapy In Vivo</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine® (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (p<0.05). Animals underwent PDZ-mediated aPDT showed complete remission of oral lesions, while animals treated with NYS presented partial remission of oral lesions in both periods assessed. Histological evaluation revealed mild inflammatory infiltrate in the groups treated with aPDT and NYS in both periods assessed. The aPDT induced the TNF-α expression when compared with the control (P-L-) (p<0.05), 24 h and 7 days after treatment. In summary, the murine model developed here was able to mimic the infection and PDZ-mediated aPDT was effective to treat mice with oral candidiasis.</description><subject>Animal models</subject><subject>Animals</subject><subject>Biofilms</subject><subject>Biology and Life Sciences</subject><subject>Cancer</subject><subject>Candida</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Candida albicans - growth & development</subject><subject>Candidiasis</subject><subject>Candidiasis, Oral - drug therapy</subject><subject>Candidiasis, Oral - genetics</subject><subject>Candidiasis, Oral - microbiology</subject><subject>Candidiasis, Oral - pathology</subject><subject>Colony Count, Microbial</subject><subject>Cytochrome</subject><subject>Cytokines</subject><subject>Data processing</subject><subject>Deactivation</subject><subject>Dental schools</subject><subject>Diabetes</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - 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drug effects</topic><topic>Candida albicans - growth & development</topic><topic>Candidiasis</topic><topic>Candidiasis, Oral - drug therapy</topic><topic>Candidiasis, Oral - genetics</topic><topic>Candidiasis, Oral - microbiology</topic><topic>Candidiasis, Oral - pathology</topic><topic>Colony Count, Microbial</topic><topic>Cytochrome</topic><topic>Cytokines</topic><topic>Data processing</topic><topic>Deactivation</topic><topic>Dental schools</topic><topic>Diabetes</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glucosamine - analogs & derivatives</topic><topic>Glucosamine - pharmacology</topic><topic>Glucosamine - therapeutic use</topic><topic>In vivo methods and tests</topic><topic>Inactivation</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Interleukin 1</topic><topic>Interleukin 6</topic><topic>Interleukin-1beta - genetics</topic><topic>Interleukin-1beta - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carmello, Juliana Cabrini</au><au>Alves, Fernanda</au><au>G Basso, Fernanda</au><au>de Souza Costa, Carlos Alberto</au><au>Bagnato, Vanderlei Salvador</au><au>Mima, Ewerton Garcia de Oliveira</au><au>Pavarina, Ana Cláudia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Treatment of Oral Candidiasis Using Photodithazine®- Mediated Photodynamic Therapy In Vivo</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-06-02</date><risdate>2016</risdate><volume>11</volume><issue>6</issue><spage>e0156947</spage><pages>e0156947-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral candidiasis in a murine model using Photodithazine® (PDZ). This model of oral candidiasis was developed to allow the monitoring of the infection and the establishment of the aPDT treatment. Six-week-old female mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDZ-mediated aPDT and nystatin treatment were carried out for 5 consecutive days with one application per day. The macroscopic evaluation of oral lesions was performed. After each treatment, the tongue was swabbed to recover C. albicans cells. Viable colonies were quantified and the number of CFU/ml determined. The animals were sacrificed 24 hours and 7 days after treatment and the tongues were surgically removed for histological analysis and analysis of inflammatory cytokines expression (IL-1, TNF-α and IL-6) by RT-qPCR. Data were analyzed by two-way ANOVA. PDZ-mediated aPDT was as effective as Nystatin (NYS group) in the inactivation of C. albicans, reducing 3 and 3.2 logs10 respectively, 24 h after treatment (p<0.05). Animals underwent PDZ-mediated aPDT showed complete remission of oral lesions, while animals treated with NYS presented partial remission of oral lesions in both periods assessed. Histological evaluation revealed mild inflammatory infiltrate in the groups treated with aPDT and NYS in both periods assessed. The aPDT induced the TNF-α expression when compared with the control (P-L-) (p<0.05), 24 h and 7 days after treatment. In summary, the murine model developed here was able to mimic the infection and PDZ-mediated aPDT was effective to treat mice with oral candidiasis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27253525</pmid><doi>10.1371/journal.pone.0156947</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-06, Vol.11 (6), p.e0156947 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1819099782 |
| source | MEDLINE; Public Library of Science; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Animal models Animals Biofilms Biology and Life Sciences Cancer Candida Candida albicans Candida albicans - drug effects Candida albicans - growth & development Candidiasis Candidiasis, Oral - drug therapy Candidiasis, Oral - genetics Candidiasis, Oral - microbiology Candidiasis, Oral - pathology Colony Count, Microbial Cytochrome Cytokines Data processing Deactivation Dental schools Diabetes Disease Models, Animal Female Gene expression Gene Expression Regulation - drug effects Glucosamine - analogs & derivatives Glucosamine - pharmacology Glucosamine - therapeutic use In vivo methods and tests Inactivation Infections Inflammation Interleukin 1 Interleukin 6 Interleukin-1beta - genetics Interleukin-1beta - metabolism Interleukin-6 - genetics Interleukin-6 - metabolism Kinetics Lasers Lesions Light Medicine and Health Sciences Mice Microorganisms Nystatin Performance evaluation Photochemotherapy Photodynamic therapy Physiology Remission Research and Analysis Methods Studies Tongue Treatment Outcome Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-TNF Tumor necrosis factor-α Variance analysis |
| title | Treatment of Oral Candidiasis Using Photodithazine®- Mediated Photodynamic Therapy In Vivo |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T16%3A01%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Treatment%20of%20Oral%20Candidiasis%20Using%20Photodithazine%C2%AE-%20Mediated%20Photodynamic%20Therapy%20In%20Vivo&rft.jtitle=PloS%20one&rft.au=Carmello,%20Juliana%20Cabrini&rft.date=2016-06-02&rft.volume=11&rft.issue=6&rft.spage=e0156947&rft.pages=e0156947-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0156947&rft_dat=%3Cproquest_plos_%3E1793905691%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1819099782&rft_id=info:pmid/27253525&rft_doaj_id=oai_doaj_org_article_9e4471ee8b4c4704ab52845753da3706&rfr_iscdi=true |