Quantitation of Murine Stroma and Selective Purification of the Human Tumor Component of Patient-Derived Xenografts for Genomic Analysis

Patient-derived xenograft (PDX) mouse models are increasingly used for preclinical therapeutic testing of human cancer. A limitation in molecular and genetic characterization of PDX tumors is the presence of integral murine stroma. This is particularly problematic for genomic sequencing of PDX model...

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Veröffentlicht in:	PloS one 2016-09, Vol.11 (9), p.e0160587-e0160587
Hauptverfasser:	Schneeberger, Valentina E, Allaj, Viola, Gardner, Eric E, Poirier, J T, Rudin, Charles M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal models Animals Bioinformatics Biology and Life Sciences Cancer Cloning Computational Biology - methods Deoxyribonucleic acid Disease Models, Animal DNA DNA sequencing Epigenetics Flow cytometry Fluorescence Gene expression Gene sequencing Genes Genetic aspects Genetic engineering Genomes Genomic analysis Genomics Genomics - methods Health aspects Heterografts High-Throughput Nucleotide Sequencing Histology Humans Lung cancer Lung diseases Medical research Medicine Medicine and Health Sciences Mice Mutation Neoplasms - genetics Neoplasms - pathology Pharmacology Polymerase chain reaction Positive selection Purification Quantitation Research and Analysis Methods Rodents Stroma Stromal Cells - metabolism Stromal Cells - pathology Tumors Virology Xenografts
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