Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge
Dogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because t...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-08, Vol.11 (8), p.e0161169-e0161169 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0161169 |
|---|---|
| container_issue | 8 |
| container_start_page | e0161169 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Resende, Lucilene Aparecida Aguiar-Soares, Rodrigo Dian de Oliveira Gama-Ker, Henrique Roatt, Bruno Mendes Mendonça, Ludmila Zanandreis de Alves, Marina Luiza Rodrigues Silveira-Lemos, Denise da Corrêa-Oliveira, Rodrigo Martins-Filho, Olindo Assis Araújo, Márcio Sobreira Silva Fujiwara, Ricardo Toshio Gontijo, Nelder Figueiredo Reis, Alexandre Barbosa Giunchetti, Rodolfo Cordeiro |
| description | Dogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in past decades. We investigated the immunogenicity of the "LbSapSal" vaccine (L. braziliensis antigens, saponin as adjuvant, and Lutzomyia longipalpis salivary gland extract) in dogs at baseline (T0), during the post-vaccination protocol (T3rd) and after early (T90) and late (T885) times following L. chagasi-challenge. Our major data indicated that immunization with "LbSapSal" is able to induce biomarkers characterized by enhanced amounts of type I (tumor necrosis factor [TNF]-α, interleukin [IL]-12, interferon [IFN]-γ) cytokines and reduction in type II cytokines (IL-4 and TGF-β), even after experimental challenge. The establishment of a prominent pro-inflammatory immune response after "LbSapSal" immunization supported the increased levels of nitric oxide production, favoring a reduction in spleen parasitism (78.9%) and indicating long-lasting protection against L. chagasi infection. In conclusion, these results confirmed the hypothesis that the "LbSapSal" vaccination is a potential tool to control the Leishmania chagasi infection. |
| doi_str_mv | 10.1371/journal.pone.0161169 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1813899704</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A461496488</galeid><doaj_id>oai_doaj_org_article_18c902706d8d490b882feba67f9ae054</doaj_id><sourcerecordid>A461496488</sourcerecordid><originalsourceid>FETCH-LOGICAL-c618t-ffd04c51e6ba8c92fd144c8f3a7587b5d9f382b500dce4613beee1f1e4a200443</originalsourceid><addsrcrecordid>eNqNklGL1DAUhYso7rr6D0QLgvgya9KmafqysAyuDgworPoabtObToc0GZNW8MH_bjrTHWbEB58Scr97cnJyk-QlJdc0L-n7rRu9BXO9cxavCeWU8upRckmrPFvwjOSPT_YXybMQtoQUueD8aXKRlUXBC8Evk9-rfgdqSJ1O1_U97O7BpN9Bqc5i2tl0CXbarfp-tM64tlOxDrZJv4CH0A3HszuEYfQY0hq187hnQA_o0zV2YdNHHUjVBtrYtVhuwBi0LT5PnmgwAV_M61Xy7e7D1-Wnxfrzx9Xydr1QnIphoXVDmCoo8hqEqjLdUMaU0DmUhSjroql0LrK6IKRRyDjNa0SkmiKDjBDG8qvk9UF3Z1yQc3JBUkFzUVUlmYjVgWgcbOXOdz34X9JBJ_cHzrcS_NApg7FLVSQrCW9EwypSC5FprIGXugIkxaR1M9821j1GS3bwYM5Ezyu228jW_ZSsqjjjJAq8mwW8-zFiGGTfBYXGgEU37n1XlGVFyf8HZfGnM1FE9M1f6L-DmKkW4ls7q120qCZReRujZdGhEJF6e0JtEMywCc6MQ-dsOAfZAVTeheBRH2OgRE5z_GBCTnMs5zmOba9OIzw2PQxu_gctd_AS</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1813899704</pqid></control><display><type>article</type><title>Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Resende, Lucilene Aparecida ; Aguiar-Soares, Rodrigo Dian de Oliveira ; Gama-Ker, Henrique ; Roatt, Bruno Mendes ; Mendonça, Ludmila Zanandreis de ; Alves, Marina Luiza Rodrigues ; Silveira-Lemos, Denise da ; Corrêa-Oliveira, Rodrigo ; Martins-Filho, Olindo Assis ; Araújo, Márcio Sobreira Silva ; Fujiwara, Ricardo Toshio ; Gontijo, Nelder Figueiredo ; Reis, Alexandre Barbosa ; Giunchetti, Rodolfo Cordeiro</creator><creatorcontrib>Resende, Lucilene Aparecida ; Aguiar-Soares, Rodrigo Dian de Oliveira ; Gama-Ker, Henrique ; Roatt, Bruno Mendes ; Mendonça, Ludmila Zanandreis de ; Alves, Marina Luiza Rodrigues ; Silveira-Lemos, Denise da ; Corrêa-Oliveira, Rodrigo ; Martins-Filho, Olindo Assis ; Araújo, Márcio Sobreira Silva ; Fujiwara, Ricardo Toshio ; Gontijo, Nelder Figueiredo ; Reis, Alexandre Barbosa ; Giunchetti, Rodolfo Cordeiro</creatorcontrib><description>Dogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in past decades. We investigated the immunogenicity of the "LbSapSal" vaccine (L. braziliensis antigens, saponin as adjuvant, and Lutzomyia longipalpis salivary gland extract) in dogs at baseline (T0), during the post-vaccination protocol (T3rd) and after early (T90) and late (T885) times following L. chagasi-challenge. Our major data indicated that immunization with "LbSapSal" is able to induce biomarkers characterized by enhanced amounts of type I (tumor necrosis factor [TNF]-α, interleukin [IL]-12, interferon [IFN]-γ) cytokines and reduction in type II cytokines (IL-4 and TGF-β), even after experimental challenge. The establishment of a prominent pro-inflammatory immune response after "LbSapSal" immunization supported the increased levels of nitric oxide production, favoring a reduction in spleen parasitism (78.9%) and indicating long-lasting protection against L. chagasi infection. In conclusion, these results confirmed the hypothesis that the "LbSapSal" vaccination is a potential tool to control the Leishmania chagasi infection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0161169</identifier><identifier>PMID: 27556586</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antigens ; Anxiety ; Biology and Life Sciences ; Biomarkers ; Brazil ; Cytokines ; Cytokines - metabolism ; Dog diseases ; Dog Diseases - immunology ; Dog Diseases - metabolism ; Dog Diseases - parasitology ; Dog Diseases - prevention & control ; Dogs ; Female ; Immune response ; Immune system ; Immunization ; Immunogenicity ; Immunology ; Infections ; Inflammation ; Inflammation Mediators - metabolism ; Interferon ; Interleukin 4 ; Leishmania ; Leishmania chagasi ; Leishmania infantum ; Leishmania infantum - immunology ; Leishmaniasis Vaccines - administration & dosage ; Leishmaniasis Vaccines - immunology ; Leishmaniasis, Visceral - veterinary ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - metabolism ; Lutzomyia longipalpis ; Lymphocytes ; Male ; Medicine and Health Sciences ; Models, Biological ; Nitric oxide ; Nitric Oxide - biosynthesis ; Parasite Load ; Parasite vaccines ; Parasites ; Parasitic diseases ; Parasitism ; Pneumoviridae ; Prevention ; Proteins ; Reduction ; Salivary gland ; Spleen ; Spleen - immunology ; Spleen - parasitology ; Testing ; Tumor necrosis factor ; Vaccination ; Vaccines ; Vector-borne diseases ; Veterinary vaccines ; Visceral leishmaniasis</subject><ispartof>PloS one, 2016-08, Vol.11 (8), p.e0161169-e0161169</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Resende et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Resende et al 2016 Resende et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-ffd04c51e6ba8c92fd144c8f3a7587b5d9f382b500dce4613beee1f1e4a200443</citedby><cites>FETCH-LOGICAL-c618t-ffd04c51e6ba8c92fd144c8f3a7587b5d9f382b500dce4613beee1f1e4a200443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996460/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996460/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27556586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Resende, Lucilene Aparecida</creatorcontrib><creatorcontrib>Aguiar-Soares, Rodrigo Dian de Oliveira</creatorcontrib><creatorcontrib>Gama-Ker, Henrique</creatorcontrib><creatorcontrib>Roatt, Bruno Mendes</creatorcontrib><creatorcontrib>Mendonça, Ludmila Zanandreis de</creatorcontrib><creatorcontrib>Alves, Marina Luiza Rodrigues</creatorcontrib><creatorcontrib>Silveira-Lemos, Denise da</creatorcontrib><creatorcontrib>Corrêa-Oliveira, Rodrigo</creatorcontrib><creatorcontrib>Martins-Filho, Olindo Assis</creatorcontrib><creatorcontrib>Araújo, Márcio Sobreira Silva</creatorcontrib><creatorcontrib>Fujiwara, Ricardo Toshio</creatorcontrib><creatorcontrib>Gontijo, Nelder Figueiredo</creatorcontrib><creatorcontrib>Reis, Alexandre Barbosa</creatorcontrib><creatorcontrib>Giunchetti, Rodolfo Cordeiro</creatorcontrib><title>Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Dogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in past decades. We investigated the immunogenicity of the "LbSapSal" vaccine (L. braziliensis antigens, saponin as adjuvant, and Lutzomyia longipalpis salivary gland extract) in dogs at baseline (T0), during the post-vaccination protocol (T3rd) and after early (T90) and late (T885) times following L. chagasi-challenge. Our major data indicated that immunization with "LbSapSal" is able to induce biomarkers characterized by enhanced amounts of type I (tumor necrosis factor [TNF]-α, interleukin [IL]-12, interferon [IFN]-γ) cytokines and reduction in type II cytokines (IL-4 and TGF-β), even after experimental challenge. The establishment of a prominent pro-inflammatory immune response after "LbSapSal" immunization supported the increased levels of nitric oxide production, favoring a reduction in spleen parasitism (78.9%) and indicating long-lasting protection against L. chagasi infection. In conclusion, these results confirmed the hypothesis that the "LbSapSal" vaccination is a potential tool to control the Leishmania chagasi infection.</description><subject>Animals</subject><subject>Antigens</subject><subject>Anxiety</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Brazil</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Dog diseases</subject><subject>Dog Diseases - immunology</subject><subject>Dog Diseases - metabolism</subject><subject>Dog Diseases - parasitology</subject><subject>Dog Diseases - prevention & control</subject><subject>Dogs</subject><subject>Female</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunology</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Interferon</subject><subject>Interleukin 4</subject><subject>Leishmania</subject><subject>Leishmania chagasi</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - immunology</subject><subject>Leishmaniasis Vaccines - administration & dosage</subject><subject>Leishmaniasis Vaccines - immunology</subject><subject>Leishmaniasis, Visceral - veterinary</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lutzomyia longipalpis</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Models, Biological</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Parasite Load</subject><subject>Parasite vaccines</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitism</subject><subject>Pneumoviridae</subject><subject>Prevention</subject><subject>Proteins</subject><subject>Reduction</subject><subject>Salivary gland</subject><subject>Spleen</subject><subject>Spleen - immunology</subject><subject>Spleen - parasitology</subject><subject>Testing</subject><subject>Tumor necrosis factor</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vector-borne diseases</subject><subject>Veterinary vaccines</subject><subject>Visceral leishmaniasis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNklGL1DAUhYso7rr6D0QLgvgya9KmafqysAyuDgworPoabtObToc0GZNW8MH_bjrTHWbEB58Scr97cnJyk-QlJdc0L-n7rRu9BXO9cxavCeWU8upRckmrPFvwjOSPT_YXybMQtoQUueD8aXKRlUXBC8Evk9-rfgdqSJ1O1_U97O7BpN9Bqc5i2tl0CXbarfp-tM64tlOxDrZJv4CH0A3HszuEYfQY0hq187hnQA_o0zV2YdNHHUjVBtrYtVhuwBi0LT5PnmgwAV_M61Xy7e7D1-Wnxfrzx9Xydr1QnIphoXVDmCoo8hqEqjLdUMaU0DmUhSjroql0LrK6IKRRyDjNa0SkmiKDjBDG8qvk9UF3Z1yQc3JBUkFzUVUlmYjVgWgcbOXOdz34X9JBJ_cHzrcS_NApg7FLVSQrCW9EwypSC5FprIGXugIkxaR1M9821j1GS3bwYM5Ezyu228jW_ZSsqjjjJAq8mwW8-zFiGGTfBYXGgEU37n1XlGVFyf8HZfGnM1FE9M1f6L-DmKkW4ls7q120qCZReRujZdGhEJF6e0JtEMywCc6MQ-dsOAfZAVTeheBRH2OgRE5z_GBCTnMs5zmOba9OIzw2PQxu_gctd_AS</recordid><startdate>20160824</startdate><enddate>20160824</enddate><creator>Resende, Lucilene Aparecida</creator><creator>Aguiar-Soares, Rodrigo Dian de Oliveira</creator><creator>Gama-Ker, Henrique</creator><creator>Roatt, Bruno Mendes</creator><creator>Mendonça, Ludmila Zanandreis de</creator><creator>Alves, Marina Luiza Rodrigues</creator><creator>Silveira-Lemos, Denise da</creator><creator>Corrêa-Oliveira, Rodrigo</creator><creator>Martins-Filho, Olindo Assis</creator><creator>Araújo, Márcio Sobreira Silva</creator><creator>Fujiwara, Ricardo Toshio</creator><creator>Gontijo, Nelder Figueiredo</creator><creator>Reis, Alexandre Barbosa</creator><creator>Giunchetti, Rodolfo Cordeiro</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160824</creationdate><title>Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge</title><author>Resende, Lucilene Aparecida ; Aguiar-Soares, Rodrigo Dian de Oliveira ; Gama-Ker, Henrique ; Roatt, Bruno Mendes ; Mendonça, Ludmila Zanandreis de ; Alves, Marina Luiza Rodrigues ; Silveira-Lemos, Denise da ; Corrêa-Oliveira, Rodrigo ; Martins-Filho, Olindo Assis ; Araújo, Márcio Sobreira Silva ; Fujiwara, Ricardo Toshio ; Gontijo, Nelder Figueiredo ; Reis, Alexandre Barbosa ; Giunchetti, Rodolfo Cordeiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-ffd04c51e6ba8c92fd144c8f3a7587b5d9f382b500dce4613beee1f1e4a200443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Anxiety</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Brazil</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Dog diseases</topic><topic>Dog Diseases - immunology</topic><topic>Dog Diseases - metabolism</topic><topic>Dog Diseases - parasitology</topic><topic>Dog Diseases - prevention & control</topic><topic>Dogs</topic><topic>Female</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunology</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Interferon</topic><topic>Interleukin 4</topic><topic>Leishmania</topic><topic>Leishmania chagasi</topic><topic>Leishmania infantum</topic><topic>Leishmania infantum - immunology</topic><topic>Leishmaniasis Vaccines - administration & dosage</topic><topic>Leishmaniasis Vaccines - immunology</topic><topic>Leishmaniasis, Visceral - veterinary</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Lutzomyia longipalpis</topic><topic>Lymphocytes</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Models, Biological</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Parasite Load</topic><topic>Parasite vaccines</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Parasitism</topic><topic>Pneumoviridae</topic><topic>Prevention</topic><topic>Proteins</topic><topic>Reduction</topic><topic>Salivary gland</topic><topic>Spleen</topic><topic>Spleen - immunology</topic><topic>Spleen - parasitology</topic><topic>Testing</topic><topic>Tumor necrosis factor</topic><topic>Vaccination</topic><topic>Vaccines</topic><topic>Vector-borne diseases</topic><topic>Veterinary vaccines</topic><topic>Visceral leishmaniasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Resende, Lucilene Aparecida</creatorcontrib><creatorcontrib>Aguiar-Soares, Rodrigo Dian de Oliveira</creatorcontrib><creatorcontrib>Gama-Ker, Henrique</creatorcontrib><creatorcontrib>Roatt, Bruno Mendes</creatorcontrib><creatorcontrib>Mendonça, Ludmila Zanandreis de</creatorcontrib><creatorcontrib>Alves, Marina Luiza Rodrigues</creatorcontrib><creatorcontrib>Silveira-Lemos, Denise da</creatorcontrib><creatorcontrib>Corrêa-Oliveira, Rodrigo</creatorcontrib><creatorcontrib>Martins-Filho, Olindo Assis</creatorcontrib><creatorcontrib>Araújo, Márcio Sobreira Silva</creatorcontrib><creatorcontrib>Fujiwara, Ricardo Toshio</creatorcontrib><creatorcontrib>Gontijo, Nelder Figueiredo</creatorcontrib><creatorcontrib>Reis, Alexandre Barbosa</creatorcontrib><creatorcontrib>Giunchetti, Rodolfo Cordeiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Resende, Lucilene Aparecida</au><au>Aguiar-Soares, Rodrigo Dian de Oliveira</au><au>Gama-Ker, Henrique</au><au>Roatt, Bruno Mendes</au><au>Mendonça, Ludmila Zanandreis de</au><au>Alves, Marina Luiza Rodrigues</au><au>Silveira-Lemos, Denise da</au><au>Corrêa-Oliveira, Rodrigo</au><au>Martins-Filho, Olindo Assis</au><au>Araújo, Márcio Sobreira Silva</au><au>Fujiwara, Ricardo Toshio</au><au>Gontijo, Nelder Figueiredo</au><au>Reis, Alexandre Barbosa</au><au>Giunchetti, Rodolfo Cordeiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-08-24</date><risdate>2016</risdate><volume>11</volume><issue>8</issue><spage>e0161169</spage><epage>e0161169</epage><pages>e0161169-e0161169</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Dogs represent the most important domestic reservoir of L. chagasi (syn. L. infantum). A vaccine against canine visceral leishmaniasis (CVL) would be an important tool for decreasing the anxiety related to possible L. chagasi infection and for controlling human visceral leishmaniasis (VL). Because the sand fly salivary proteins are potent immunogens obligatorily co-deposited during transmission of Leishmania parasites, their inclusion in an anti-Leishmania vaccine has been investigated in past decades. We investigated the immunogenicity of the "LbSapSal" vaccine (L. braziliensis antigens, saponin as adjuvant, and Lutzomyia longipalpis salivary gland extract) in dogs at baseline (T0), during the post-vaccination protocol (T3rd) and after early (T90) and late (T885) times following L. chagasi-challenge. Our major data indicated that immunization with "LbSapSal" is able to induce biomarkers characterized by enhanced amounts of type I (tumor necrosis factor [TNF]-α, interleukin [IL]-12, interferon [IFN]-γ) cytokines and reduction in type II cytokines (IL-4 and TGF-β), even after experimental challenge. The establishment of a prominent pro-inflammatory immune response after "LbSapSal" immunization supported the increased levels of nitric oxide production, favoring a reduction in spleen parasitism (78.9%) and indicating long-lasting protection against L. chagasi infection. In conclusion, these results confirmed the hypothesis that the "LbSapSal" vaccination is a potential tool to control the Leishmania chagasi infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27556586</pmid><doi>10.1371/journal.pone.0161169</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-08, Vol.11 (8), p.e0161169-e0161169 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1813899704 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Antigens Anxiety Biology and Life Sciences Biomarkers Brazil Cytokines Cytokines - metabolism Dog diseases Dog Diseases - immunology Dog Diseases - metabolism Dog Diseases - parasitology Dog Diseases - prevention & control Dogs Female Immune response Immune system Immunization Immunogenicity Immunology Infections Inflammation Inflammation Mediators - metabolism Interferon Interleukin 4 Leishmania Leishmania chagasi Leishmania infantum Leishmania infantum - immunology Leishmaniasis Vaccines - administration & dosage Leishmaniasis Vaccines - immunology Leishmaniasis, Visceral - veterinary Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Lutzomyia longipalpis Lymphocytes Male Medicine and Health Sciences Models, Biological Nitric oxide Nitric Oxide - biosynthesis Parasite Load Parasite vaccines Parasites Parasitic diseases Parasitism Pneumoviridae Prevention Proteins Reduction Salivary gland Spleen Spleen - immunology Spleen - parasitology Testing Tumor necrosis factor Vaccination Vaccines Vector-borne diseases Veterinary vaccines Visceral leishmaniasis |
| title | Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T22%3A00%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impact%20of%20LbSapSal%20Vaccine%20in%20Canine%20Immunological%20and%20Parasitological%20Features%20before%20and%20after%20Leishmania%20chagasi-Challenge&rft.jtitle=PloS%20one&rft.au=Resende,%20Lucilene%20Aparecida&rft.date=2016-08-24&rft.volume=11&rft.issue=8&rft.spage=e0161169&rft.epage=e0161169&rft.pages=e0161169-e0161169&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0161169&rft_dat=%3Cgale_plos_%3EA461496488%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1813899704&rft_id=info:pmid/27556586&rft_galeid=A461496488&rft_doaj_id=oai_doaj_org_article_18c902706d8d490b882feba67f9ae054&rfr_iscdi=true |