Vaccine Potential and Diversity of the Putative Cell Binding Factor (CBF, NMB0345/NEIS1825) Protein of Neisseria meningitidis

The cbf gene from Neisseria meningitidis strain MC58 encoding the putative Cell Binding Factor (CBF, NMB0345/NEIS1825) protein was cloned into the pRSETA system and a ~36-kDa recombinant (r)CBF protein expressed in Escherichia coli and purified by metal affinity chromatography. High titres of rCBF a...

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Veröffentlicht in:	PloS one 2016-08, Vol.11 (8), p.e0160403-e0160403
Hauptverfasser:	Humbert, María Victoria, Hung, Miao-Chiu, Phillips, Renee, Akoto, Charlene, Hill, Alison, Tan, Wei-Ming, Heckels, John Edward, Christodoulides, Myron
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Affinity chromatography Alleles Amino Acid Sequence Amino acids Analysis Animals Antibodies Antibodies, Bacterial - immunology Antisera Bacteria Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - immunology Bactericidal activity Binding Biodiversity Biology and Life Sciences CBF gene CBF protein Chromatography Cloning Computational Biology Conservation Conserved sequence E coli Escherichia coli Flow cytometry Genomes Health aspects Homology Immune response Immune Sera - immunology Immunity, Humoral Immunization Infections Laboratories Lipid A Liposomes Lysates Medicine Medicine and Health Sciences Meningococcal Vaccines - chemistry Meningococcal Vaccines - genetics Meningococcal Vaccines - immunology Mice Micelles Models, Molecular Monophosphoryl lipid A Neisseria Neisseria meningitidis Neisseria meningitidis - immunology Pathogenesis Protein Conformation Proteins Proteomics Research and Analysis Methods Serum bactericidal activity Strains (organisms) Vaccination Vaccines Western blotting
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