Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome
The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive forestry pest rapidly expanding in many countries. This pest poses a considerable threat to the urban forestry ecosystem, especially to Platanus spp. However, its molecular biology and biochemistry are poorly understood....
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| Veröffentlicht in: | PloS one 2016-08, Vol.11 (8), p.e0160609-e0160609 |
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| creator | Li, Fengqi Wang, Ran Qu, Cheng Fu, Ningning Luo, Chen Xu, Yihua |
| description | The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive forestry pest rapidly expanding in many countries. This pest poses a considerable threat to the urban forestry ecosystem, especially to Platanus spp. However, its molecular biology and biochemistry are poorly understood. This study reports the first C. ciliata transcriptome, encompassing three different life stages (Nymphs, adults female (AF) and adults male (AM)). In total, 26.53 GB of clean data and 60,879 unigenes were obtained from three RNA-seq libraries. These unigenes were annotated and classified by Nr (NCBI non-redundant protein sequences), Nt (NCBI non-redundant nucleotide sequences), Pfam (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (A manually annotated and reviewed protein sequence database), and KO (KEGG Ortholog database). After all pairwise comparisons between these three different samples, a large number of differentially expressed genes were revealed. The dramatic differences in global gene expression profiles were found between distinct life stages (nymphs and AF, nymphs and AM) and sex difference (AF and AM), with some of the significantly differentially expressed genes (DEGs) being related to metamorphosis, digestion, immune and sex difference. The different express of unigenes were validated through quantitative Real-Time PCR (qRT-PCR) for 16 randomly selected unigenes. In addition, 17,462 potential simple sequence repeat molecular markers were identified in these transcriptome resources. These comprehensive C. ciliata transcriptomic information can be utilized to promote the development of environmentally friendly methodologies to disrupt the processes of metamorphosis, digestion, immune and sex differences. |
| doi_str_mv | 10.1371/journal.pone.0160609 |
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This pest poses a considerable threat to the urban forestry ecosystem, especially to Platanus spp. However, its molecular biology and biochemistry are poorly understood. This study reports the first C. ciliata transcriptome, encompassing three different life stages (Nymphs, adults female (AF) and adults male (AM)). In total, 26.53 GB of clean data and 60,879 unigenes were obtained from three RNA-seq libraries. These unigenes were annotated and classified by Nr (NCBI non-redundant protein sequences), Nt (NCBI non-redundant nucleotide sequences), Pfam (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (A manually annotated and reviewed protein sequence database), and KO (KEGG Ortholog database). After all pairwise comparisons between these three different samples, a large number of differentially expressed genes were revealed. The dramatic differences in global gene expression profiles were found between distinct life stages (nymphs and AF, nymphs and AM) and sex difference (AF and AM), with some of the significantly differentially expressed genes (DEGs) being related to metamorphosis, digestion, immune and sex difference. The different express of unigenes were validated through quantitative Real-Time PCR (qRT-PCR) for 16 randomly selected unigenes. In addition, 17,462 potential simple sequence repeat molecular markers were identified in these transcriptome resources. These comprehensive C. ciliata transcriptomic information can be utilized to promote the development of environmentally friendly methodologies to disrupt the processes of metamorphosis, digestion, immune and sex differences.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0160609</identifier><identifier>PMID: 27494615</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adults ; Agriculture ; Amino acid sequence ; Animals ; Biochemistry ; Bioinformatics ; Biology and Life Sciences ; Ciliata ; Computer programs ; Corythucha ciliata ; Deoxyribonucleic acid ; Digestion ; DNA ; DNA polymerase ; DNA sequencing ; Forest ecosystems ; Forestry ; Gender aspects ; Gene expression ; Genes ; Genetic aspects ; Genomes ; Hemiptera ; Hemiptera - genetics ; High-Throughput Nucleotide Sequencing - methods ; Information processing ; Insect pests ; Insect Proteins - genetics ; Insecticides ; Metamorphosis ; Molecular biology ; Molecular Sequence Annotation ; Pests ; Physiological aspects ; Platanaceae ; Platanus ; Proteins ; Real-Time Polymerase Chain Reaction ; Research and Analysis Methods ; Reviews ; Ribonucleic acid ; RNA ; Sex ; Sex differences ; Tingidae ; Transcriptome - genetics</subject><ispartof>PloS one, 2016-08, Vol.11 (8), p.e0160609-e0160609</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Li et al 2016 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-ee338df56c8bfec75925bec9361a1de5d6fecb5144a89cbc5a1073c6652236ac3</citedby><cites>FETCH-LOGICAL-c791t-ee338df56c8bfec75925bec9361a1de5d6fecb5144a89cbc5a1073c6652236ac3</cites><orcidid>0000-0001-6476-9116</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975459/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975459/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27494615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhang, Youjun</contributor><creatorcontrib>Li, Fengqi</creatorcontrib><creatorcontrib>Wang, Ran</creatorcontrib><creatorcontrib>Qu, Cheng</creatorcontrib><creatorcontrib>Fu, Ningning</creatorcontrib><creatorcontrib>Luo, Chen</creatorcontrib><creatorcontrib>Xu, Yihua</creatorcontrib><title>Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive forestry pest rapidly expanding in many countries. 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These comprehensive C. ciliata transcriptomic information can be utilized to promote the development of environmentally friendly methodologies to disrupt the processes of metamorphosis, digestion, immune and sex differences.</description><subject>Adults</subject><subject>Agriculture</subject><subject>Amino acid sequence</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biology and Life Sciences</subject><subject>Ciliata</subject><subject>Computer programs</subject><subject>Corythucha ciliata</subject><subject>Deoxyribonucleic acid</subject><subject>Digestion</subject><subject>DNA</subject><subject>DNA polymerase</subject><subject>DNA sequencing</subject><subject>Forest ecosystems</subject><subject>Forestry</subject><subject>Gender aspects</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Hemiptera</subject><subject>Hemiptera - genetics</subject><subject>High-Throughput Nucleotide Sequencing - methods</subject><subject>Information processing</subject><subject>Insect pests</subject><subject>Insect Proteins - genetics</subject><subject>Insecticides</subject><subject>Metamorphosis</subject><subject>Molecular biology</subject><subject>Molecular Sequence Annotation</subject><subject>Pests</subject><subject>Physiological aspects</subject><subject>Platanaceae</subject><subject>Platanus</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Research and Analysis Methods</subject><subject>Reviews</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sex</subject><subject>Sex differences</subject><subject>Tingidae</subject><subject>Transcriptome - genetics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99v0zAQxyMEYmPwHyCwhIS2hxY7_pGEB6RRAatUaRIrvFoX59J4SuNiJ9XGX4-7ZdOK9jD5wdbd574-3_mS5C2jU8Yz9unSDb6DdrpxHU4pU1TR4llyyAqeTlRK-fMH54PkVQiXlEqeK_UyOUgzUQjF5GFydYF_BuyM7VYEuorMGvBgevT2L_TWdcTVpG-QzLstBLtFcnFtYO08kgUYJF-HFZk5f903g2mAGNta6IEcn-HabqIKfCbLKG0rwBOy9NAF46PDrfF18qKGNuCbcT9Kfn3_tpydTRbnP-az08XEZAXrJ4ic51UtlcnLGk0mi1SWaAquGLAKZaWitZRMCMgLUxoJjGbcKCXTlCsw_Ch5f6u7aV3QY9GCZjlLJcuEyiMxvyUqB5d64-0a_LV2YPWNwfmVBt9b06LOJbIqKwteVqXIOC-UBGHSHEqQKYCIWl_G24ZyjZXBrvfQ7onuezrb6JXbalFkUsgiChyPAt7FxoRer20w2LbQoRtu8pYZYzlPn4JSLlWR8oh--A99vBAjtYL4VtvVLqZodqL6VChKORX5jpo-QsVVxZ6b-BlrG-17ASd7AZHp8apfwRCCnl_8fDp7_nuf_fiAbRDavgmuHXbfNuyD4hY03oXgsb7vB6N6N0t31dC7WdLjLMWwdw97eR90Nzz8HxlEGgc</recordid><startdate>20160805</startdate><enddate>20160805</enddate><creator>Li, Fengqi</creator><creator>Wang, Ran</creator><creator>Qu, Cheng</creator><creator>Fu, Ningning</creator><creator>Luo, Chen</creator><creator>Xu, Yihua</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6476-9116</orcidid></search><sort><creationdate>20160805</creationdate><title>Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome</title><author>Li, Fengqi ; Wang, Ran ; Qu, Cheng ; Fu, Ningning ; Luo, Chen ; Xu, Yihua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c791t-ee338df56c8bfec75925bec9361a1de5d6fecb5144a89cbc5a1073c6652236ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adults</topic><topic>Agriculture</topic><topic>Amino acid sequence</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Bioinformatics</topic><topic>Biology and Life Sciences</topic><topic>Ciliata</topic><topic>Computer programs</topic><topic>Corythucha ciliata</topic><topic>Deoxyribonucleic acid</topic><topic>Digestion</topic><topic>DNA</topic><topic>DNA polymerase</topic><topic>DNA sequencing</topic><topic>Forest ecosystems</topic><topic>Forestry</topic><topic>Gender aspects</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Hemiptera</topic><topic>Hemiptera - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Fengqi</au><au>Wang, Ran</au><au>Qu, Cheng</au><au>Fu, Ningning</au><au>Luo, Chen</au><au>Xu, Yihua</au><au>Zhang, Youjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-08-05</date><risdate>2016</risdate><volume>11</volume><issue>8</issue><spage>e0160609</spage><epage>e0160609</epage><pages>e0160609-e0160609</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae), is an invasive forestry pest rapidly expanding in many countries. This pest poses a considerable threat to the urban forestry ecosystem, especially to Platanus spp. However, its molecular biology and biochemistry are poorly understood. This study reports the first C. ciliata transcriptome, encompassing three different life stages (Nymphs, adults female (AF) and adults male (AM)). In total, 26.53 GB of clean data and 60,879 unigenes were obtained from three RNA-seq libraries. These unigenes were annotated and classified by Nr (NCBI non-redundant protein sequences), Nt (NCBI non-redundant nucleotide sequences), Pfam (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (A manually annotated and reviewed protein sequence database), and KO (KEGG Ortholog database). After all pairwise comparisons between these three different samples, a large number of differentially expressed genes were revealed. The dramatic differences in global gene expression profiles were found between distinct life stages (nymphs and AF, nymphs and AM) and sex difference (AF and AM), with some of the significantly differentially expressed genes (DEGs) being related to metamorphosis, digestion, immune and sex difference. The different express of unigenes were validated through quantitative Real-Time PCR (qRT-PCR) for 16 randomly selected unigenes. In addition, 17,462 potential simple sequence repeat molecular markers were identified in these transcriptome resources. These comprehensive C. ciliata transcriptomic information can be utilized to promote the development of environmentally friendly methodologies to disrupt the processes of metamorphosis, digestion, immune and sex differences.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27494615</pmid><doi>10.1371/journal.pone.0160609</doi><tpages>e0160609</tpages><orcidid>https://orcid.org/0000-0001-6476-9116</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adults Agriculture Amino acid sequence Animals Biochemistry Bioinformatics Biology and Life Sciences Ciliata Computer programs Corythucha ciliata Deoxyribonucleic acid Digestion DNA DNA polymerase DNA sequencing Forest ecosystems Forestry Gender aspects Gene expression Genes Genetic aspects Genomes Hemiptera Hemiptera - genetics High-Throughput Nucleotide Sequencing - methods Information processing Insect pests Insect Proteins - genetics Insecticides Metamorphosis Molecular biology Molecular Sequence Annotation Pests Physiological aspects Platanaceae Platanus Proteins Real-Time Polymerase Chain Reaction Research and Analysis Methods Reviews Ribonucleic acid RNA Sex Sex differences Tingidae Transcriptome - genetics |
| title | Sequencing and Characterization of the Invasive Sycamore Lace Bug Corythucha ciliata (Hemiptera: Tingidae) Transcriptome |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T14%3A33%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sequencing%20and%20Characterization%20of%20the%20Invasive%20Sycamore%20Lace%20Bug%20Corythucha%20ciliata%20(Hemiptera:%20Tingidae)%20Transcriptome&rft.jtitle=PloS%20one&rft.au=Li,%20Fengqi&rft.date=2016-08-05&rft.volume=11&rft.issue=8&rft.spage=e0160609&rft.epage=e0160609&rft.pages=e0160609-e0160609&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0160609&rft_dat=%3Cgale_plos_%3EA460030488%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1812517468&rft_id=info:pmid/27494615&rft_galeid=A460030488&rft_doaj_id=oai_doaj_org_article_85e1d7b93bdb4733965a4c28aba52aa4&rfr_iscdi=true |