Global DNA Methylation Changes in Nile Tilapia Gonads during High Temperature-Induced Masculinization
In some fish species, high or low temperature can switch the sex determination mechanisms and induce fish sex reversal when the gonads are undifferentiated. During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation chang...
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| description | In some fish species, high or low temperature can switch the sex determination mechanisms and induce fish sex reversal when the gonads are undifferentiated. During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing. The DNA methylation level in CF was higher than that in CM for various chromosomes. Both females and males showed an increase in methylation levels on various chromosomes after high-temperature induction. We identified 64,438 (CF/CM), 63,437 (TF/IM), 98,675 (TF/CF), 235,270 (IM/CM) and 119,958 (IM/CF) differentially methylated regions (DMRs) in Nile tilapia gonads, representing approximately 0.70% (CF/CM), 0.69% (TF/IM), 1.07% (TF/CF), 2.56% (IM/CM), and 1.30% (IM/CF)of the length of the genome. A total of 89 and 65 genes that exhibited DMRs in their gene bodies and promoters were mapped to the Nile tilapia genome. Furthermore, more than half of the genes with DMRs in the gene body in CF/CM were also included in the IM/CM, TF/CF, TF/IM, and IM/CF groups. Additionally, many important pathways, including neuroactive ligand-receptor interaction, extracellular matrix-receptor interaction, and biosynthesis of unsaturated fatty acids were identified. This study provided an important foundation to investigate the molecular mechanism of high temperature-induced sex reversal in fish species. |
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During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing. The DNA methylation level in CF was higher than that in CM for various chromosomes. Both females and males showed an increase in methylation levels on various chromosomes after high-temperature induction. We identified 64,438 (CF/CM), 63,437 (TF/IM), 98,675 (TF/CF), 235,270 (IM/CM) and 119,958 (IM/CF) differentially methylated regions (DMRs) in Nile tilapia gonads, representing approximately 0.70% (CF/CM), 0.69% (TF/IM), 1.07% (TF/CF), 2.56% (IM/CM), and 1.30% (IM/CF)of the length of the genome. A total of 89 and 65 genes that exhibited DMRs in their gene bodies and promoters were mapped to the Nile tilapia genome. Furthermore, more than half of the genes with DMRs in the gene body in CF/CM were also included in the IM/CM, TF/CF, TF/IM, and IM/CF groups. Additionally, many important pathways, including neuroactive ligand-receptor interaction, extracellular matrix-receptor interaction, and biosynthesis of unsaturated fatty acids were identified. This study provided an important foundation to investigate the molecular mechanism of high temperature-induced sex reversal in fish species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0158483</identifier><identifier>PMID: 27486872</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal sciences ; Animals ; Biology and life sciences ; Biosynthesis ; Chromosomes ; Chromosomes - genetics ; Cichlids - genetics ; Comparative analysis ; Deoxyribonucleic acid ; DNA ; DNA Methylation ; DNA sequencing ; Epigenetics ; Extracellular matrix ; Fatty acids ; Female ; Females ; Fish ; Fishes ; Gene expression ; Gene Expression Regulation ; Gene Regulatory Networks ; Gene sequencing ; Genes ; Genomes ; Genomics ; Gonads ; Gonads - ultrastructure ; High temperature ; Histology ; Hot Temperature ; Immunoprecipitation ; Low temperature ; Male ; Males ; Medicine and Health Sciences ; Methylation ; Morphology ; Oreochromis niloticus ; Physiological aspects ; Research and Analysis Methods ; Sequence Analysis, DNA ; Sex ; Sex determination ; Sex Determination Processes ; Sex reversal ; Temperature effects ; Tilapia ; Unsaturated fatty acids ; Zoology</subject><ispartof>PloS one, 2016-08, Vol.11 (8), p.e0158483-e0158483</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing. The DNA methylation level in CF was higher than that in CM for various chromosomes. Both females and males showed an increase in methylation levels on various chromosomes after high-temperature induction. We identified 64,438 (CF/CM), 63,437 (TF/IM), 98,675 (TF/CF), 235,270 (IM/CM) and 119,958 (IM/CF) differentially methylated regions (DMRs) in Nile tilapia gonads, representing approximately 0.70% (CF/CM), 0.69% (TF/IM), 1.07% (TF/CF), 2.56% (IM/CM), and 1.30% (IM/CF)of the length of the genome. A total of 89 and 65 genes that exhibited DMRs in their gene bodies and promoters were mapped to the Nile tilapia genome. Furthermore, more than half of the genes with DMRs in the gene body in CF/CM were also included in the IM/CM, TF/CF, TF/IM, and IM/CF groups. Additionally, many important pathways, including neuroactive ligand-receptor interaction, extracellular matrix-receptor interaction, and biosynthesis of unsaturated fatty acids were identified. This study provided an important foundation to investigate the molecular mechanism of high temperature-induced sex reversal in fish species.</description><subject>Animal sciences</subject><subject>Animals</subject><subject>Biology and life sciences</subject><subject>Biosynthesis</subject><subject>Chromosomes</subject><subject>Chromosomes - genetics</subject><subject>Cichlids - genetics</subject><subject>Comparative analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Methylation</subject><subject>DNA sequencing</subject><subject>Epigenetics</subject><subject>Extracellular matrix</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Females</subject><subject>Fish</subject><subject>Fishes</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene Regulatory Networks</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Gonads</subject><subject>Gonads - ultrastructure</subject><subject>High temperature</subject><subject>Histology</subject><subject>Hot Temperature</subject><subject>Immunoprecipitation</subject><subject>Low temperature</subject><subject>Male</subject><subject>Males</subject><subject>Medicine and Health Sciences</subject><subject>Methylation</subject><subject>Morphology</subject><subject>Oreochromis niloticus</subject><subject>Physiological aspects</subject><subject>Research and Analysis Methods</subject><subject>Sequence Analysis, DNA</subject><subject>Sex</subject><subject>Sex determination</subject><subject>Sex Determination Processes</subject><subject>Sex reversal</subject><subject>Temperature effects</subject><subject>Tilapia</subject><subject>Unsaturated fatty acids</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYqPwDxBEQkJw0WInju3cIE0Dukr7kKBwa53EJ6kr1-7iBDF-Pe7aTS3axeQLW_ZzXp_PJHlNyYTmgn5a-qFzYCdr73BCaCGZzJ8kx7TMszHPSP5073yUvAhhSUiRS86fJ0eZYJJLkR0nOLW-Apt-uTxJL7Bf3FjojXfp6QJciyE1Lr00FtO5sbA2kE69Ax1SPXTGtemZaRfpHFdr7KAfOhzPnB5q1OkFhHqwxpm_t3Ivk2cN2ICvdvso-fnt6_z0bHx-NZ2dnpyPa5EV_bhiXAsugAGlDREIKEvArKKsKEEIqERTFVzSTEjJdKVlqWMkTZnpqgHQLB8lb7e6a-uD2mUoKCqJ5ITyjEZitiW0h6Vad2YF3Y3yYNTthe9aBV1vaouKCWiKkmlCsWJZWUCFVFcFYRkrUXIRtT7vfhuqFeoaXd-BPRA9fHFmoVr_W7FSZDnPo8CHnUDnrwcMvVqZUKO14NAPG78pLQmN8T4CJSUnBeebEN_9hz6ciB3VQozVuMZHF-uNqDphghJa0ujiKJk8QMWlcWXq2HlNbI5Dg48HBpHp8U_fwhCCmv34_nj26tch-36PXSDYfhG8HTbdFQ5BtgXrzofQYXNfD0rUZnDusqE2g6N2gxPN3uzX8t7oblLyfwGUEiY</recordid><startdate>20160803</startdate><enddate>20160803</enddate><creator>Sun, Li-Xue</creator><creator>Wang, Yi-Ya</creator><creator>Zhao, Yan</creator><creator>Wang, Hui</creator><creator>Li, Ning</creator><creator>Ji, Xiang Shan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3285-7952</orcidid></search><sort><creationdate>20160803</creationdate><title>Global DNA Methylation Changes in Nile Tilapia Gonads during High Temperature-Induced Masculinization</title><author>Sun, Li-Xue ; Wang, Yi-Ya ; Zhao, Yan ; Wang, Hui ; Li, Ning ; Ji, Xiang Shan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-b46d767a4a11f07eae89ae2b1459a77ab7fb568127884dbd89d274f92dbfaad43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animal sciences</topic><topic>Animals</topic><topic>Biology and life sciences</topic><topic>Biosynthesis</topic><topic>Chromosomes</topic><topic>Chromosomes - 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During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing. The DNA methylation level in CF was higher than that in CM for various chromosomes. Both females and males showed an increase in methylation levels on various chromosomes after high-temperature induction. We identified 64,438 (CF/CM), 63,437 (TF/IM), 98,675 (TF/CF), 235,270 (IM/CM) and 119,958 (IM/CF) differentially methylated regions (DMRs) in Nile tilapia gonads, representing approximately 0.70% (CF/CM), 0.69% (TF/IM), 1.07% (TF/CF), 2.56% (IM/CM), and 1.30% (IM/CF)of the length of the genome. A total of 89 and 65 genes that exhibited DMRs in their gene bodies and promoters were mapped to the Nile tilapia genome. Furthermore, more than half of the genes with DMRs in the gene body in CF/CM were also included in the IM/CM, TF/CF, TF/IM, and IM/CF groups. Additionally, many important pathways, including neuroactive ligand-receptor interaction, extracellular matrix-receptor interaction, and biosynthesis of unsaturated fatty acids were identified. This study provided an important foundation to investigate the molecular mechanism of high temperature-induced sex reversal in fish species.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27486872</pmid><doi>10.1371/journal.pone.0158483</doi><tpages>e0158483</tpages><orcidid>https://orcid.org/0000-0002-3285-7952</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animal sciences Animals Biology and life sciences Biosynthesis Chromosomes Chromosomes - genetics Cichlids - genetics Comparative analysis Deoxyribonucleic acid DNA DNA Methylation DNA sequencing Epigenetics Extracellular matrix Fatty acids Female Females Fish Fishes Gene expression Gene Expression Regulation Gene Regulatory Networks Gene sequencing Genes Genomes Genomics Gonads Gonads - ultrastructure High temperature Histology Hot Temperature Immunoprecipitation Low temperature Male Males Medicine and Health Sciences Methylation Morphology Oreochromis niloticus Physiological aspects Research and Analysis Methods Sequence Analysis, DNA Sex Sex determination Sex Determination Processes Sex reversal Temperature effects Tilapia Unsaturated fatty acids Zoology |
| title | Global DNA Methylation Changes in Nile Tilapia Gonads during High Temperature-Induced Masculinization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T04%3A22%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Global%20DNA%20Methylation%20Changes%20in%20Nile%20Tilapia%20Gonads%20during%20High%20Temperature-Induced%20Masculinization&rft.jtitle=PloS%20one&rft.au=Sun,%20Li-Xue&rft.date=2016-08-03&rft.volume=11&rft.issue=8&rft.spage=e0158483&rft.epage=e0158483&rft.pages=e0158483-e0158483&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0158483&rft_dat=%3Cgale_plos_%3EA471019136%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1808601621&rft_id=info:pmid/27486872&rft_galeid=A471019136&rft_doaj_id=oai_doaj_org_article_47af594d01eb4295abe1db504249e867&rfr_iscdi=true |