Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.)
Serine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs...
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| creator | Li, Zhiyong Tang, Liqun Qiu, Jiehua Zhang, Wen Wang, Yifeng Tong, Xiaohong Wei, Xiangjin Hou, Yuxuan Zhang, Jian |
| description | Serine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice. |
| doi_str_mv | 10.1371/journal.pone.0159737 |
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To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0159737</identifier><identifier>PMID: 27448032</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abscisic acid ; Analysis ; Arabidopsis ; Biology and Life Sciences ; Carboxypeptidase ; Carboxypeptidases - genetics ; Carboxypeptidases - metabolism ; Cluster Analysis ; Dormancy ; Endosperm ; Enzymes ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant - drug effects ; Genetic aspects ; Genome, Plant ; Genomes ; Germination ; Germination - genetics ; Grain ; Growth ; Kinases ; Molecular biology ; Organ Specificity - genetics ; Oryza - physiology ; Oryza sativa ; Plant genetics ; Plant Growth Regulators - pharmacology ; Proteins ; Proteolysis ; Research and Analysis Methods ; Ribonucleic acid ; Rice ; RNA ; Seed germination ; Seeds ; Seeds - physiology ; Serine ; Serine carboxypeptidase ; Signaling ; Transcriptome ; Yeast ; Zea mays - genetics</subject><ispartof>PloS one, 2016-07, Vol.11 (7), p.e0159737-e0159737</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Li et al 2016 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-db04647dce8f1898a530b8f82b26046b8c9aee8ff4813db1f0a99619abcb81433</citedby><cites>FETCH-LOGICAL-c725t-db04647dce8f1898a530b8f82b26046b8c9aee8ff4813db1f0a99619abcb81433</cites><orcidid>0000-0003-2804-0162</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957776/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957776/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27448032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zhiyong</creatorcontrib><creatorcontrib>Tang, Liqun</creatorcontrib><creatorcontrib>Qiu, Jiehua</creatorcontrib><creatorcontrib>Zhang, Wen</creatorcontrib><creatorcontrib>Wang, Yifeng</creatorcontrib><creatorcontrib>Tong, Xiaohong</creatorcontrib><creatorcontrib>Wei, Xiangjin</creatorcontrib><creatorcontrib>Hou, Yuxuan</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><title>Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Serine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice.</description><subject>Abscisic acid</subject><subject>Analysis</subject><subject>Arabidopsis</subject><subject>Biology and Life Sciences</subject><subject>Carboxypeptidase</subject><subject>Carboxypeptidases - genetics</subject><subject>Carboxypeptidases - metabolism</subject><subject>Cluster Analysis</subject><subject>Dormancy</subject><subject>Endosperm</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genetic aspects</subject><subject>Genome, Plant</subject><subject>Genomes</subject><subject>Germination</subject><subject>Germination - genetics</subject><subject>Grain</subject><subject>Growth</subject><subject>Kinases</subject><subject>Molecular biology</subject><subject>Organ Specificity - genetics</subject><subject>Oryza - physiology</subject><subject>Oryza sativa</subject><subject>Plant genetics</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Proteins</subject><subject>Proteolysis</subject><subject>Research and Analysis Methods</subject><subject>Ribonucleic acid</subject><subject>Rice</subject><subject>RNA</subject><subject>Seed germination</subject><subject>Seeds</subject><subject>Seeds - physiology</subject><subject>Serine</subject><subject>Serine carboxypeptidase</subject><subject>Signaling</subject><subject>Transcriptome</subject><subject>Yeast</subject><subject>Zea mays - genetics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk21r2zAQx83YWLtu32BshsFoXyTTg62HN4NS1qwQCDTb3oqzfE5VHCuT7NLs009Z0pKMvigCSdz97q_TSZdl7ykZUy7pl1s_hA7a8cp3OCa01JLLF9kx1ZyNBCP85d7-KHsT4y0hJVdCvM6OmCwKRTg7zmCOwXWYWwiVv1-vcNW7GiLmhcivcTG00GPMJwFcl1-6tnXdIoeuzueIdT7BsHQd9M53efJfO4v56Sys_0Aek_UO8un47G32qoE24rvdepL9vPz24-L7aDqbXF2cT0dWsrIf1RUpRCFri6qhSisoOalUo1jFRPJUymrA5GsKRXld0YaA1oJqqGylaMH5SfZxq7tqfTS76kRDFRGMc8bKRFxtidrDrVkFt4SwNh6c-WfwYWEg9M62aATBwlYgEVEUrGwUEqt0U1lGSxBKJ62vu9OGaokp664P0B6IHno6d2MW_s4UupRSiiRwuhMI_veAsTdLFy22LXToh03elCpVCvEclEgmdZoS-uk_9OlC7KgFpLu6rvEpRbsRNedFqYkgmspEjZ-g0qhx6Wz6dY1L9oOAs4OAxPR43y9giNFcza-fz85-HbKf99gbhLa_ib4dNv8uHoLFFrTBxxiweXwPSsymaR6qYTZNY3ZNk8I-7L_lY9BDl_C_woYQAw</recordid><startdate>20160722</startdate><enddate>20160722</enddate><creator>Li, Zhiyong</creator><creator>Tang, Liqun</creator><creator>Qiu, Jiehua</creator><creator>Zhang, Wen</creator><creator>Wang, Yifeng</creator><creator>Tong, Xiaohong</creator><creator>Wei, Xiangjin</creator><creator>Hou, Yuxuan</creator><creator>Zhang, Jian</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2804-0162</orcidid></search><sort><creationdate>20160722</creationdate><title>Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.)</title><author>Li, Zhiyong ; Tang, Liqun ; Qiu, Jiehua ; Zhang, Wen ; Wang, Yifeng ; Tong, Xiaohong ; Wei, Xiangjin ; Hou, Yuxuan ; Zhang, Jian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-db04647dce8f1898a530b8f82b26046b8c9aee8ff4813db1f0a99619abcb81433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Abscisic acid</topic><topic>Analysis</topic><topic>Arabidopsis</topic><topic>Biology and Life Sciences</topic><topic>Carboxypeptidase</topic><topic>Carboxypeptidases - genetics</topic><topic>Carboxypeptidases - metabolism</topic><topic>Cluster Analysis</topic><topic>Dormancy</topic><topic>Endosperm</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genetic aspects</topic><topic>Genome, Plant</topic><topic>Genomes</topic><topic>Germination</topic><topic>Germination - genetics</topic><topic>Grain</topic><topic>Growth</topic><topic>Kinases</topic><topic>Molecular biology</topic><topic>Organ Specificity - genetics</topic><topic>Oryza - physiology</topic><topic>Oryza sativa</topic><topic>Plant genetics</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Proteins</topic><topic>Proteolysis</topic><topic>Research and Analysis Methods</topic><topic>Ribonucleic acid</topic><topic>Rice</topic><topic>RNA</topic><topic>Seed germination</topic><topic>Seeds</topic><topic>Seeds - physiology</topic><topic>Serine</topic><topic>Serine carboxypeptidase</topic><topic>Signaling</topic><topic>Transcriptome</topic><topic>Yeast</topic><topic>Zea mays - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhiyong</au><au>Tang, Liqun</au><au>Qiu, Jiehua</au><au>Zhang, Wen</au><au>Wang, Yifeng</au><au>Tong, Xiaohong</au><au>Wei, Xiangjin</au><au>Hou, Yuxuan</au><au>Zhang, Jian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-07-22</date><risdate>2016</risdate><volume>11</volume><issue>7</issue><spage>e0159737</spage><epage>e0159737</epage><pages>e0159737-e0159737</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Serine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27448032</pmid><doi>10.1371/journal.pone.0159737</doi><tpages>e0159737</tpages><orcidid>https://orcid.org/0000-0003-2804-0162</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abscisic acid Analysis Arabidopsis Biology and Life Sciences Carboxypeptidase Carboxypeptidases - genetics Carboxypeptidases - metabolism Cluster Analysis Dormancy Endosperm Enzymes Gene expression Gene Expression Profiling Gene Expression Regulation, Plant - drug effects Genetic aspects Genome, Plant Genomes Germination Germination - genetics Grain Growth Kinases Molecular biology Organ Specificity - genetics Oryza - physiology Oryza sativa Plant genetics Plant Growth Regulators - pharmacology Proteins Proteolysis Research and Analysis Methods Ribonucleic acid Rice RNA Seed germination Seeds Seeds - physiology Serine Serine carboxypeptidase Signaling Transcriptome Yeast Zea mays - genetics |
| title | Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.) |
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