In Silico Design and Experimental Validation of siRNAs Targeting Conserved Regions of Multiple Hepatitis C Virus Genotypes

RNA interference (RNAi) is a post-transcriptional gene silencing mechanism that mediates the sequence-specific degradation of targeted RNA and thus provides a tremendous opportunity for development of oligonucleotide-based drugs. Here, we report on the design and validation of small interfering RNAs...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2016-07, Vol.11 (7), p.e0159211-e0159211
Hauptverfasser:	ElHefnawi, Mahmoud, Kim, TaeKyu, Kamar, Mona A, Min, Saehong, Hassan, Nafisa M, El-Ahwany, Eman, Kim, Heeyoung, Zada, Suher, Amer, Marwa, Windisch, Marc P
Format:	Artikel
Sprache:	eng
Schlagworte:	Accessibility Antiviral agents Antiviral Agents - pharmacology Binding Sites Biology Biology and life sciences Care and treatment Cell culture Cell Line, Tumor Cell lines Computer Simulation Conserved Sequence - genetics Design Drug development Drugs Effectiveness Gene expression Gene sequencing Gene silencing Genetic aspects Genome, Viral Genomes Genotype Genotype & phenotype Genotypes Green Fluorescent Proteins - metabolism Hepacivirus - drug effects Hepacivirus - genetics Hepatitis Hepatitis C Hepatitis C virus Humans Informatics Interferon Internal ribosome entry site Internal Ribosome Entry Sites - genetics Laboratories Liver cancer Low concentrations Luciferases - metabolism Medicine and health sciences Nucleic Acid Conformation Nucleotide sequence Oligonucleotides Physiological aspects Post-transcription Proteins Recombinant Fusion Proteins - metabolism Replication Replicon - genetics Reproducibility of Results Research and Analysis Methods Ribonucleic acid RNA RNA interference RNA viruses RNA, Small Interfering - therapeutic use RNA-mediated interference RNAi Therapeutics Science Side effects siRNA Therapeutic applications Time Factors Viral Nonstructural Proteins Virus Replication - drug effects Viruses
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!