Antioxidant Activity and ROS-Dependent Apoptotic Effect of Scurrula ferruginea (Jack) Danser Methanol Extract in Human Breast Cancer Cell MDA-MB-231
Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, w...
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| Veröffentlicht in: | PloS one 2016-07, Vol.11 (7), p.e0158942-e0158942 |
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| creator | Marvibaigi, Mohsen Amini, Neda Supriyanto, Eko Abdul Majid, Fadzilah Adibah Kumar Jaganathan, Saravana Jamil, Shajarahtunnur Hamzehalipour Almaki, Javad Nasiri, Rozita |
| description | Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, which showed potent antioxidant activities, and high phenolic and flavonoid contents, were investigated in human breast cancer cell line (MDA-MB-231) and non-cancer human skin fibroblast cells (HSF-1184). The activities and characteristics varied depending on the different parts of S. ferruginea, solvent polarity, and concentrations of extracts. The stem methanol extract showed the highest amount of both phenolic (273.51 ± 4.84 mg gallic acid/g extract) and flavonoid contents (163.41 ± 4.62 mg catechin/g extract) and strong DPPH• radical scavenging (IC50 = 27.81 μg/mL) and metal chelation activity (IC50 = 80.20 μg/mL). The stem aqueous extract showed the highest ABTS•+ scavenging ability. The stem methanol and aqueous extracts exhibited dose-dependent cytotoxic activity against MDA-MB-231 cells with IC50 of 19.27 and 50.35 μg/mL, respectively. Furthermore, the extracts inhibited the migration and colony formation of MDA-MB-231 cells in a concentration-dependent manner. Morphological observations revealed hallmark properties of apoptosis in treated cells. The methanol extract induced an increase in ROS generation and mitochondrial depolarization in MDA-MB-231 cells, suggesting its potent apoptotic activity. The present study demonstrated that the S. ferruginea methanol extract mediated MDA-MB-231 cell growth inhibition via induction of apoptosis which was confirmed by Western blot analysis. It may be a potential anticancer agent; however, its in vivo anticancer activity needs to be investigated. |
| doi_str_mv | 10.1371/journal.pone.0158942 |
| format | Article |
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This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, which showed potent antioxidant activities, and high phenolic and flavonoid contents, were investigated in human breast cancer cell line (MDA-MB-231) and non-cancer human skin fibroblast cells (HSF-1184). The activities and characteristics varied depending on the different parts of S. ferruginea, solvent polarity, and concentrations of extracts. The stem methanol extract showed the highest amount of both phenolic (273.51 ± 4.84 mg gallic acid/g extract) and flavonoid contents (163.41 ± 4.62 mg catechin/g extract) and strong DPPH• radical scavenging (IC50 = 27.81 μg/mL) and metal chelation activity (IC50 = 80.20 μg/mL). The stem aqueous extract showed the highest ABTS•+ scavenging ability. The stem methanol and aqueous extracts exhibited dose-dependent cytotoxic activity against MDA-MB-231 cells with IC50 of 19.27 and 50.35 μg/mL, respectively. Furthermore, the extracts inhibited the migration and colony formation of MDA-MB-231 cells in a concentration-dependent manner. Morphological observations revealed hallmark properties of apoptosis in treated cells. The methanol extract induced an increase in ROS generation and mitochondrial depolarization in MDA-MB-231 cells, suggesting its potent apoptotic activity. The present study demonstrated that the S. ferruginea methanol extract mediated MDA-MB-231 cell growth inhibition via induction of apoptosis which was confirmed by Western blot analysis. It may be a potential anticancer agent; however, its in vivo anticancer activity needs to be investigated.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0158942</identifier><identifier>PMID: 27410459</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anticancer properties ; Antioxidants ; Antioxidants (Nutrients) ; Antioxidants - chemistry ; Antioxidants - isolation & purification ; Antioxidants - metabolism ; Antioxidants - pharmacology ; Antitumor activity ; Apoptosis ; Apoptosis - drug effects ; Biology and Life Sciences ; Biphenyl Compounds - metabolism ; Branches ; Breast cancer ; Breast Neoplasms - pathology ; Cancer ; Care and treatment ; Caspase 3 - metabolism ; Caspase 7 - metabolism ; Catechin ; Cell Line, Tumor ; Cell Movement - drug effects ; Cell Nucleus - drug effects ; Cell Proliferation - drug effects ; Chelation ; Cytotoxicity ; Deciduous trees ; Depolarization ; Drug therapy ; Flavonoids - analysis ; Gallic acid ; Humans ; In vivo methods and tests ; Iron - metabolism ; Loranthaceae ; Medicine and Health Sciences ; Membrane Potential, Mitochondrial - drug effects ; Methanol ; Methanol - chemistry ; Mistletoes ; Mitochondria ; Phenolic compounds ; Phenols ; Phenols - analysis ; Physical Sciences ; Picrates - metabolism ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - metabolism ; Plant Extracts - pharmacology ; Plant Stems - chemistry ; Polarity ; Poly(ADP-ribose) Polymerases - metabolism ; Reactive Oxygen Species - metabolism ; Research and Analysis Methods ; Risk factors ; Santalales ; Scavenging ; Skin ; Tracheophyta - chemistry ; Viscum album ; Water - chemistry</subject><ispartof>PloS one, 2016-07, Vol.11 (7), p.e0158942-e0158942</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Marvibaigi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Marvibaigi et al 2016 Marvibaigi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-8ceb2d40070820c155e2271f2b7730665828fd16269ec811fb2511e99f17575d3</citedby><cites>FETCH-LOGICAL-c725t-8ceb2d40070820c155e2271f2b7730665828fd16269ec811fb2511e99f17575d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943642/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943642/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27410459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lebedeva, Irina V</contributor><creatorcontrib>Marvibaigi, Mohsen</creatorcontrib><creatorcontrib>Amini, Neda</creatorcontrib><creatorcontrib>Supriyanto, Eko</creatorcontrib><creatorcontrib>Abdul Majid, Fadzilah Adibah</creatorcontrib><creatorcontrib>Kumar Jaganathan, Saravana</creatorcontrib><creatorcontrib>Jamil, Shajarahtunnur</creatorcontrib><creatorcontrib>Hamzehalipour Almaki, Javad</creatorcontrib><creatorcontrib>Nasiri, Rozita</creatorcontrib><title>Antioxidant Activity and ROS-Dependent Apoptotic Effect of Scurrula ferruginea (Jack) Danser Methanol Extract in Human Breast Cancer Cell MDA-MB-231</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, which showed potent antioxidant activities, and high phenolic and flavonoid contents, were investigated in human breast cancer cell line (MDA-MB-231) and non-cancer human skin fibroblast cells (HSF-1184). The activities and characteristics varied depending on the different parts of S. ferruginea, solvent polarity, and concentrations of extracts. The stem methanol extract showed the highest amount of both phenolic (273.51 ± 4.84 mg gallic acid/g extract) and flavonoid contents (163.41 ± 4.62 mg catechin/g extract) and strong DPPH• radical scavenging (IC50 = 27.81 μg/mL) and metal chelation activity (IC50 = 80.20 μg/mL). The stem aqueous extract showed the highest ABTS•+ scavenging ability. The stem methanol and aqueous extracts exhibited dose-dependent cytotoxic activity against MDA-MB-231 cells with IC50 of 19.27 and 50.35 μg/mL, respectively. Furthermore, the extracts inhibited the migration and colony formation of MDA-MB-231 cells in a concentration-dependent manner. Morphological observations revealed hallmark properties of apoptosis in treated cells. The methanol extract induced an increase in ROS generation and mitochondrial depolarization in MDA-MB-231 cells, suggesting its potent apoptotic activity. The present study demonstrated that the S. ferruginea methanol extract mediated MDA-MB-231 cell growth inhibition via induction of apoptosis which was confirmed by Western blot analysis. It may be a potential anticancer agent; however, its in vivo anticancer activity needs to be investigated.</description><subject>Anticancer properties</subject><subject>Antioxidants</subject><subject>Antioxidants (Nutrients)</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - metabolism</subject><subject>Antioxidants - pharmacology</subject><subject>Antitumor activity</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biology and Life Sciences</subject><subject>Biphenyl Compounds - metabolism</subject><subject>Branches</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 7 - metabolism</subject><subject>Catechin</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Cell Nucleus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marvibaigi, Mohsen</au><au>Amini, Neda</au><au>Supriyanto, Eko</au><au>Abdul Majid, Fadzilah Adibah</au><au>Kumar Jaganathan, Saravana</au><au>Jamil, Shajarahtunnur</au><au>Hamzehalipour Almaki, Javad</au><au>Nasiri, Rozita</au><au>Lebedeva, Irina V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant Activity and ROS-Dependent Apoptotic Effect of Scurrula ferruginea (Jack) Danser Methanol Extract in Human Breast Cancer Cell MDA-MB-231</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-07-13</date><risdate>2016</risdate><volume>11</volume><issue>7</issue><spage>e0158942</spage><epage>e0158942</epage><pages>e0158942-e0158942</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Scurrula ferruginea (Jack) Danser is one of the mistletoe species belonging to Loranthaceae family, which grows on the branches of many deciduous trees in tropical countries. This study evaluated the antioxidant activities of S. ferruginea extracts. The cytotoxic activity of the selected extracts, which showed potent antioxidant activities, and high phenolic and flavonoid contents, were investigated in human breast cancer cell line (MDA-MB-231) and non-cancer human skin fibroblast cells (HSF-1184). The activities and characteristics varied depending on the different parts of S. ferruginea, solvent polarity, and concentrations of extracts. The stem methanol extract showed the highest amount of both phenolic (273.51 ± 4.84 mg gallic acid/g extract) and flavonoid contents (163.41 ± 4.62 mg catechin/g extract) and strong DPPH• radical scavenging (IC50 = 27.81 μg/mL) and metal chelation activity (IC50 = 80.20 μg/mL). The stem aqueous extract showed the highest ABTS•+ scavenging ability. The stem methanol and aqueous extracts exhibited dose-dependent cytotoxic activity against MDA-MB-231 cells with IC50 of 19.27 and 50.35 μg/mL, respectively. Furthermore, the extracts inhibited the migration and colony formation of MDA-MB-231 cells in a concentration-dependent manner. Morphological observations revealed hallmark properties of apoptosis in treated cells. The methanol extract induced an increase in ROS generation and mitochondrial depolarization in MDA-MB-231 cells, suggesting its potent apoptotic activity. The present study demonstrated that the S. ferruginea methanol extract mediated MDA-MB-231 cell growth inhibition via induction of apoptosis which was confirmed by Western blot analysis. It may be a potential anticancer agent; however, its in vivo anticancer activity needs to be investigated.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27410459</pmid><doi>10.1371/journal.pone.0158942</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-07, Vol.11 (7), p.e0158942-e0158942 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1803789597 |
| source | Electronic Journals Library; MEDLINE; PLoS_OA刊; PubMed Central; Directory of Open Access Journals; Free Full-Text Journals in Chemistry |
| subjects | Anticancer properties Antioxidants Antioxidants (Nutrients) Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - metabolism Antioxidants - pharmacology Antitumor activity Apoptosis Apoptosis - drug effects Biology and Life Sciences Biphenyl Compounds - metabolism Branches Breast cancer Breast Neoplasms - pathology Cancer Care and treatment Caspase 3 - metabolism Caspase 7 - metabolism Catechin Cell Line, Tumor Cell Movement - drug effects Cell Nucleus - drug effects Cell Proliferation - drug effects Chelation Cytotoxicity Deciduous trees Depolarization Drug therapy Flavonoids - analysis Gallic acid Humans In vivo methods and tests Iron - metabolism Loranthaceae Medicine and Health Sciences Membrane Potential, Mitochondrial - drug effects Methanol Methanol - chemistry Mistletoes Mitochondria Phenolic compounds Phenols Phenols - analysis Physical Sciences Picrates - metabolism Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - metabolism Plant Extracts - pharmacology Plant Stems - chemistry Polarity Poly(ADP-ribose) Polymerases - metabolism Reactive Oxygen Species - metabolism Research and Analysis Methods Risk factors Santalales Scavenging Skin Tracheophyta - chemistry Viscum album Water - chemistry |
| title | Antioxidant Activity and ROS-Dependent Apoptotic Effect of Scurrula ferruginea (Jack) Danser Methanol Extract in Human Breast Cancer Cell MDA-MB-231 |
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