Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats
The present study was conducted over a course of 104 weeks to estimate the carcinogenicity of ethanol-extracted Brazilian green propolis (EEP). Groups of 50 male and 50 female Wistar Hannover rats, 6-week-old at commencement were exposed to EEP at doses of 0, 0.5 or 2.5% in the diet. Survival rates...
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| description | The present study was conducted over a course of 104 weeks to estimate the carcinogenicity of ethanol-extracted Brazilian green propolis (EEP). Groups of 50 male and 50 female Wistar Hannover rats, 6-week-old at commencement were exposed to EEP at doses of 0, 0.5 or 2.5% in the diet. Survival rates of 0.5% and 2.5% EEP-treated male and female rats, respectively, were significantly higher than those of respective control groups. Overall histopathological evaluation of neoplasms in rat tissues after 2 years showed no significant increase of tumors or preneoplastic lesions in any organ of animals administered EEP. Significantly lower incidences of pituitary tumors in 0.5% EEP male and 2.5% EEP female groups, malignant lymphoma/leukemia in both 2.5% EEP-treated males and females and total thyroid tumors in 0.5% EEP male group were found. Administration of EEP caused significant decreases of lymphoid hyperplasia of the thymus and lymph nodes in 2.5% EEP-treated rats, tubular cell hyperplasia of kidneys in all EEP groups, and cortical hyperplasia of adrenals in EEP-treated females. In the blood, significant reduction of neutrophils in all EEP-treated males and band neutrophils in 2.5% EEP-treated females was found indicating lower levels of inflammation. Total cholesterol and triglicerides levels were significantly lower in the blood of 2.5% EEP-treated female rats. In conclusion, under the conditions of the 2-year feeding experiment, EEP was not carcinogenic, did not induce significant histopathological changes in any organ, and further exerted anti-inflammatory and antitumorigenic effects resulting in increase of survival of Wistar Hannover rats. |
| doi_str_mv | 10.1371/journal.pone.0158654 |
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Groups of 50 male and 50 female Wistar Hannover rats, 6-week-old at commencement were exposed to EEP at doses of 0, 0.5 or 2.5% in the diet. Survival rates of 0.5% and 2.5% EEP-treated male and female rats, respectively, were significantly higher than those of respective control groups. Overall histopathological evaluation of neoplasms in rat tissues after 2 years showed no significant increase of tumors or preneoplastic lesions in any organ of animals administered EEP. Significantly lower incidences of pituitary tumors in 0.5% EEP male and 2.5% EEP female groups, malignant lymphoma/leukemia in both 2.5% EEP-treated males and females and total thyroid tumors in 0.5% EEP male group were found. Administration of EEP caused significant decreases of lymphoid hyperplasia of the thymus and lymph nodes in 2.5% EEP-treated rats, tubular cell hyperplasia of kidneys in all EEP groups, and cortical hyperplasia of adrenals in EEP-treated females. In the blood, significant reduction of neutrophils in all EEP-treated males and band neutrophils in 2.5% EEP-treated females was found indicating lower levels of inflammation. Total cholesterol and triglicerides levels were significantly lower in the blood of 2.5% EEP-treated female rats. In conclusion, under the conditions of the 2-year feeding experiment, EEP was not carcinogenic, did not induce significant histopathological changes in any organ, and further exerted anti-inflammatory and antitumorigenic effects resulting in increase of survival of Wistar Hannover rats.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0158654</identifier><identifier>PMID: 27391589</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Animal tissues ; Animals ; Apoptosis ; Biology and Life Sciences ; Bladder ; Blood ; Body Weight - drug effects ; Brain cancer ; Brain tumors ; Cancer ; Carcinogenesis - drug effects ; Carcinogenicity ; Carcinogens ; Carcinogens - toxicity ; Care and treatment ; Cell growth ; Cell Transformation, Neoplastic ; Cholesterol ; Cytokines ; Diet ; Drug dosages ; Enzymes ; Ethanol ; Ethanol - chemistry ; Female ; Females ; Hyperplasia ; Inflammation ; Kidneys ; Laboratory animals ; Lesions ; Leukemia ; Leukocytes (neutrophilic) ; Lymph nodes ; Lymphoma ; Male ; Males ; Medicine ; Medicine and Health Sciences ; Mutagenicity Tests ; Neoplasia ; Neoplasms ; Pathology ; Physiological aspects ; Pituitary ; Pituitary gland ; Plant extracts ; Plant Extracts - chemistry ; Properties ; Propolis ; Propolis - chemistry ; Prostate ; Rats ; Rats, Wistar ; Rodents ; Survival ; Thymus ; Thyroid ; Thyroid cancer ; Tumor necrosis factor-TNF ; Tumorigenesis ; Tumors ; University graduates</subject><ispartof>PloS one, 2016-07, Vol.11 (7), p.e0158654</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Kakehashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Kakehashi et al 2016 Kakehashi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-9d3f89398c69eafbec6b53101006eaf7dd5ffb125242401777bc618815efe3f13</citedby><cites>FETCH-LOGICAL-c791t-9d3f89398c69eafbec6b53101006eaf7dd5ffb125242401777bc618815efe3f13</cites><orcidid>0000-0003-1149-1450</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938237/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938237/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27391589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kakehashi, Anna</creatorcontrib><creatorcontrib>Ishii, Naomi</creatorcontrib><creatorcontrib>Fujioka, Masaki</creatorcontrib><creatorcontrib>Doi, Kenichiro</creatorcontrib><creatorcontrib>Gi, Min</creatorcontrib><creatorcontrib>Wanibuchi, Hideki</creatorcontrib><title>Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The present study was conducted over a course of 104 weeks to estimate the carcinogenicity of ethanol-extracted Brazilian green propolis (EEP). Groups of 50 male and 50 female Wistar Hannover rats, 6-week-old at commencement were exposed to EEP at doses of 0, 0.5 or 2.5% in the diet. Survival rates of 0.5% and 2.5% EEP-treated male and female rats, respectively, were significantly higher than those of respective control groups. Overall histopathological evaluation of neoplasms in rat tissues after 2 years showed no significant increase of tumors or preneoplastic lesions in any organ of animals administered EEP. Significantly lower incidences of pituitary tumors in 0.5% EEP male and 2.5% EEP female groups, malignant lymphoma/leukemia in both 2.5% EEP-treated males and females and total thyroid tumors in 0.5% EEP male group were found. Administration of EEP caused significant decreases of lymphoid hyperplasia of the thymus and lymph nodes in 2.5% EEP-treated rats, tubular cell hyperplasia of kidneys in all EEP groups, and cortical hyperplasia of adrenals in EEP-treated females. In the blood, significant reduction of neutrophils in all EEP-treated males and band neutrophils in 2.5% EEP-treated females was found indicating lower levels of inflammation. Total cholesterol and triglicerides levels were significantly lower in the blood of 2.5% EEP-treated female rats. In conclusion, under the conditions of the 2-year feeding experiment, EEP was not carcinogenic, did not induce significant histopathological changes in any organ, and further exerted anti-inflammatory and antitumorigenic effects resulting in increase of survival of Wistar Hannover rats.</description><subject>Acids</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biology and Life Sciences</subject><subject>Bladder</subject><subject>Blood</subject><subject>Body Weight - drug effects</subject><subject>Brain cancer</subject><subject>Brain tumors</subject><subject>Cancer</subject><subject>Carcinogenesis - drug effects</subject><subject>Carcinogenicity</subject><subject>Carcinogens</subject><subject>Carcinogens - toxicity</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cholesterol</subject><subject>Cytokines</subject><subject>Diet</subject><subject>Drug dosages</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>Ethanol - chemistry</subject><subject>Female</subject><subject>Females</subject><subject>Hyperplasia</subject><subject>Inflammation</subject><subject>Kidneys</subject><subject>Laboratory animals</subject><subject>Lesions</subject><subject>Leukemia</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lymph nodes</subject><subject>Lymphoma</subject><subject>Male</subject><subject>Males</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mutagenicity Tests</subject><subject>Neoplasia</subject><subject>Neoplasms</subject><subject>Pathology</subject><subject>Physiological aspects</subject><subject>Pituitary</subject><subject>Pituitary gland</subject><subject>Plant extracts</subject><subject>Plant Extracts - chemistry</subject><subject>Properties</subject><subject>Propolis</subject><subject>Propolis - chemistry</subject><subject>Prostate</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Survival</subject><subject>Thymus</subject><subject>Thyroid</subject><subject>Thyroid cancer</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>University graduates</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk19v0zAUxSMEYmPwDRBEQkLw0GLHcZy8II2psEqThsaAR8txrlNPqV1spyp8epw1mxq0B5QH__udk9zj3CR5idEcE4Y_3NjeGdHNN9bAHGFaFjR_lBzjimSzIkPk8cH8KHnm_Q1ClJRF8TQ5yhipoqI6TtpFWAlju9liF5yQAZr0kxN_dKeFSb86u7Gd9uliBy74YR1ABr2FdKFUnPnUmvS6X1unWzDgI6pN-lP7IFx6LoyxW3DplQj-efJEic7Di3E8Sb5_Xlyfnc8uLr8sz04vZpJVOMyqhqiyIlUpiwqEqkEWNSUYYYSKuGZNQ5WqcUazPMsRZozVssBliSkoIAqTk-T13nfTWc_HjDzHJcpiQjjPIrHcE40VN3zj9Fq439wKzW83rGu5cEHLDjirVI4RzTOGUC5pWcssp6LKAARjNC-i18fxbX29hkaCiSF2E9PpidEr3totzytSZoRFg3ejgbO_evCBr7WX0HXCgO1vv7ssCGJ0qOzNP-jD1Y1UK2IB2ig7XOtgyk9zyghlrKSRmj9AxaeBtZbxh1I67k8E7yeCyATYhVb03vPlt6v_Zy9_TNm3B-wKRBdW3nZ90Nb4KZjvQems9w7UfcgY8aEf7tLgQz_wsR-i7NXhBd2L7hqA_AUo0QV7</recordid><startdate>20160708</startdate><enddate>20160708</enddate><creator>Kakehashi, Anna</creator><creator>Ishii, Naomi</creator><creator>Fujioka, Masaki</creator><creator>Doi, Kenichiro</creator><creator>Gi, Min</creator><creator>Wanibuchi, Hideki</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1149-1450</orcidid></search><sort><creationdate>20160708</creationdate><title>Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats</title><author>Kakehashi, Anna ; Ishii, Naomi ; Fujioka, Masaki ; Doi, Kenichiro ; Gi, Min ; Wanibuchi, Hideki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c791t-9d3f89398c69eafbec6b53101006eaf7dd5ffb125242401777bc618815efe3f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acids</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biology and Life Sciences</topic><topic>Bladder</topic><topic>Blood</topic><topic>Body Weight - 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Groups of 50 male and 50 female Wistar Hannover rats, 6-week-old at commencement were exposed to EEP at doses of 0, 0.5 or 2.5% in the diet. Survival rates of 0.5% and 2.5% EEP-treated male and female rats, respectively, were significantly higher than those of respective control groups. Overall histopathological evaluation of neoplasms in rat tissues after 2 years showed no significant increase of tumors or preneoplastic lesions in any organ of animals administered EEP. Significantly lower incidences of pituitary tumors in 0.5% EEP male and 2.5% EEP female groups, malignant lymphoma/leukemia in both 2.5% EEP-treated males and females and total thyroid tumors in 0.5% EEP male group were found. Administration of EEP caused significant decreases of lymphoid hyperplasia of the thymus and lymph nodes in 2.5% EEP-treated rats, tubular cell hyperplasia of kidneys in all EEP groups, and cortical hyperplasia of adrenals in EEP-treated females. In the blood, significant reduction of neutrophils in all EEP-treated males and band neutrophils in 2.5% EEP-treated females was found indicating lower levels of inflammation. Total cholesterol and triglicerides levels were significantly lower in the blood of 2.5% EEP-treated female rats. In conclusion, under the conditions of the 2-year feeding experiment, EEP was not carcinogenic, did not induce significant histopathological changes in any organ, and further exerted anti-inflammatory and antitumorigenic effects resulting in increase of survival of Wistar Hannover rats.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27391589</pmid><doi>10.1371/journal.pone.0158654</doi><orcidid>https://orcid.org/0000-0003-1149-1450</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2016-07, Vol.11 (7), p.e0158654 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1802586142 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Animal tissues Animals Apoptosis Biology and Life Sciences Bladder Blood Body Weight - drug effects Brain cancer Brain tumors Cancer Carcinogenesis - drug effects Carcinogenicity Carcinogens Carcinogens - toxicity Care and treatment Cell growth Cell Transformation, Neoplastic Cholesterol Cytokines Diet Drug dosages Enzymes Ethanol Ethanol - chemistry Female Females Hyperplasia Inflammation Kidneys Laboratory animals Lesions Leukemia Leukocytes (neutrophilic) Lymph nodes Lymphoma Male Males Medicine Medicine and Health Sciences Mutagenicity Tests Neoplasia Neoplasms Pathology Physiological aspects Pituitary Pituitary gland Plant extracts Plant Extracts - chemistry Properties Propolis Propolis - chemistry Prostate Rats Rats, Wistar Rodents Survival Thymus Thyroid Thyroid cancer Tumor necrosis factor-TNF Tumorigenesis Tumors University graduates |
| title | Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T00%3A58%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ethanol-Extracted%20Brazilian%20Propolis%20Exerts%20Protective%20Effects%20on%20Tumorigenesis%20in%20Wistar%20Hannover%20Rats&rft.jtitle=PloS%20one&rft.au=Kakehashi,%20Anna&rft.date=2016-07-08&rft.volume=11&rft.issue=7&rft.spage=e0158654&rft.pages=e0158654-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0158654&rft_dat=%3Cgale_plos_%3EA457357785%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1802586142&rft_id=info:pmid/27391589&rft_galeid=A457357785&rft_doaj_id=oai_doaj_org_article_79f4105427004c58bc245a92eea77546&rfr_iscdi=true |