Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool

To establish a novel protocol for differentiation of retinal pigment epithelium (RPE) with high purity from mouse induced pluripotent stem cells (iPSC). Retinal progenitor cells were differentiated from mouse iPSC, and RPE differentiation was then enhanced by activation of the Wnt signaling pathway,...

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Veröffentlicht in:	PloS one 2016-07, Vol.11 (7), p.e0158282-e0158282
Hauptverfasser:	Iwasaki, Yuko, Sugita, Sunao, Mandai, Michiko, Yonemura, Shigenobu, Onishi, Akishi, Ito, Shin-Ichiro, Mochizuki, Manabu, Ohno-Matsui, Kyoko, Takahashi, Masayo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens Biology and Life Sciences Cell Communication Cell Culture Techniques Cell Differentiation Cell Proliferation Cells (biology) Cells, Cultured Cellular structure Collagenases - chemistry Differentiation Electron microscopy Embryonic Stem Cells - cytology Epithelium Fibroblast growth factors Gene expression Genetic aspects Immunocytochemistry Induced Pluripotent Stem Cells - cytology Inhibition Kinases Lymphocytes - cytology Medicine and Health Sciences Mice Neural stem cells Peptide Hydrolases - chemistry Phagocytosis Physical Sciences Physiological aspects Plates (structural members) Pluripotency Polarity Progenitor cells Protein kinase Purification Purity Research and Analysis Methods Retina Retinal pigment epithelium Retinal Pigment Epithelium - metabolism rho-Associated Kinases - metabolism Rod Cell Outer Segment - metabolism Rodents Signal Transduction Signaling Stem cell transplantation Stem cells Tight junctions Wnt protein
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