Expression of Inflammatory and Cell Death Program Genes and Comet DNA Damage Assay Induced by Escherichia coli in Layer Hens
Modern methods of industrial poultry and egg production systems involve stressful practices that stimulate Escherichia coli (E. coli) activity causing endotoxic shock. This investigation was conducted to evaluate the expression of pro-inflammatory cytokines and cell death program genes and DNA damag...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-06, Vol.11 (6), p.e0158314-e0158314 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0158314 |
|---|---|
| container_issue | 6 |
| container_start_page | e0158314 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Mehaisen, Gamal M K Eshak, Mariam G El Sabry, M I Abass, Ahmed O |
| description | Modern methods of industrial poultry and egg production systems involve stressful practices that stimulate Escherichia coli (E. coli) activity causing endotoxic shock. This investigation was conducted to evaluate the expression of pro-inflammatory cytokines and cell death program genes and DNA damage induced by E. coli in the brain and liver tissues of laying hens. A total of two hundred and ten H&N brown layer hens with 20 week age, were used in this research. First, preliminary experiments were designed (60 hens in total) to establish the optimal exposure dose of E. coli and to determine the nearest time of notable response to be used in the remainder studies of this research. At 35-wk of age, 150 hens were randomly assigned into 2 groups with 3 replicates of 25 birds each; the first group was injected in the brachial wing vein with 107 E. coli colony/hen, while the second group was injected with saline and served as a control. The body temperature and plasma corticosterone concentration were measured 3 hr after injection. Specimens of liver and brain were obtained from each group and the gene expression of p38 mitogen-activated protein kinase, interlukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), Bax, and caspase-3 genes were measured by quantitative real-time PCR. DNA damage in the brain and liver tissues were also measured by comet assay. Hens treated with E. coli showed significant (P |
| doi_str_mv | 10.1371/journal.pone.0158314 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1799851978</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A456433544</galeid><doaj_id>oai_doaj_org_article_c21de4a61ecb4363ad010f2deaa37d18</doaj_id><sourcerecordid>A456433544</sourcerecordid><originalsourceid>FETCH-LOGICAL-c684t-d54ad8ce3cc3add5b6b21c4db1e04d9351c6b23bf80cd6088327484403c88bc53</originalsourceid><addsrcrecordid>eNqNkkGP0zAQhSMEYpeFf4DAEhLi0mLHjuNckKq27FaqgAOcrYk9aVMlcbETRCV-PC7NrlrEgZMtzzfPz-OXJC8ZnTKes_c7N_gOmunedTilLFOciUfJNSt4OpEp5Y_P9lfJsxB2lGZcSfk0uUpzLnKZF9fJr-XPvccQatcRV5FVVzXQttA7fyDQWTLHpiELhH5Lvni38dCSW-wwnIquxZ4sPs3IAlrYIJmFAIcoYgeDlpQHsgxmi7422xqIcU1N6o6s4YCe3GEXnidPKmgCvhjXm-Tbx-XX-d1k_fl2NZ-tJ0Yq0U9sJsAqg9wYDtZmpSxTZoQtGVJhC54xE094WSlqrKRK8TQXSgjKjVKlyfhN8vqku29c0OPggmZ5UaiMFbmKxOpEWAc7vfd1C_6gHdT6z4HzGw2-r02D2qTMogDJ0JSCy-iIMlqlFgF4btlR68N421C2aA12vYfmQvSy0tVbvXE_tCjSNMvSKPBuFPDu-4Ch120dTPwI6NAN0beiSnKa5fR_UCpoyqSM6Ju_0H8PYqQ2EN9ad5WLFs1RVM9EJgXnmRCRentGbRGafhtcM_QxRuESFCfQeBeCx-phDIzqY4zvTehjjPUY49j26nyED033ueW_ARur7m8</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1799851978</pqid></control><display><type>article</type><title>Expression of Inflammatory and Cell Death Program Genes and Comet DNA Damage Assay Induced by Escherichia coli in Layer Hens</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>Open Access: PubMed Central</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Free Full-Text Journals in Chemistry</source><source>EZB Electronic Journals Library</source><creator>Mehaisen, Gamal M K ; Eshak, Mariam G ; El Sabry, M I ; Abass, Ahmed O</creator><creatorcontrib>Mehaisen, Gamal M K ; Eshak, Mariam G ; El Sabry, M I ; Abass, Ahmed O</creatorcontrib><description>Modern methods of industrial poultry and egg production systems involve stressful practices that stimulate Escherichia coli (E. coli) activity causing endotoxic shock. This investigation was conducted to evaluate the expression of pro-inflammatory cytokines and cell death program genes and DNA damage induced by E. coli in the brain and liver tissues of laying hens. A total of two hundred and ten H&N brown layer hens with 20 week age, were used in this research. First, preliminary experiments were designed (60 hens in total) to establish the optimal exposure dose of E. coli and to determine the nearest time of notable response to be used in the remainder studies of this research. At 35-wk of age, 150 hens were randomly assigned into 2 groups with 3 replicates of 25 birds each; the first group was injected in the brachial wing vein with 107 E. coli colony/hen, while the second group was injected with saline and served as a control. The body temperature and plasma corticosterone concentration were measured 3 hr after injection. Specimens of liver and brain were obtained from each group and the gene expression of p38 mitogen-activated protein kinase, interlukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), Bax, and caspase-3 genes were measured by quantitative real-time PCR. DNA damage in the brain and liver tissues were also measured by comet assay. Hens treated with E. coli showed significant (P<0.05) increase of body temperature and plasma corticosterone (42.6°C and 14.5 ng/ml, respectively) compared to the control group (41.1°C and 5.5 ng/ml, respectively). Additional remarkable over-inflammation gene expression of p38, IL-1β and TNF-α.genes were also detected in the brain (2.2-fold, 2.0-fold and 3.3-fold, respectively) and the liver (2.1-fold, 1.9-fold and 3.0-fold, respectively) tissues of the infected chickens. It is also important to note that hens injected with E. coli showed an increase in DNA damage in the brain and liver cells (P<0.05). These results were synchronized with activating cell death program since our data showed significant high expression of Bax gene by 2.8- and 2.7-fold and caspase-3 gene by 2.5- and 2.7-fold in the brain and liver tissues of infected chickens, respectively (P<0.05). In conclusion, the current study indicates that E. coli injection induces inflammatory physiological response and triggers cell death program in the brain and liver. Our results provide more understanding to endotoxic shock by E. coli in chickens at cellular level. Further studies are required to confirm if such responses are destructive or protective to set the means through which a chicken mounts a successful defense against avian pathogenic E. coli.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0158314</identifier><identifier>PMID: 27347679</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal tissues ; Animals ; Apoptosis ; Bacteria ; Bacterial infections ; BAX gene ; Bax protein ; Bioassay ; Bioassays ; Biology and Life Sciences ; Biomarkers ; Birds ; Body Temperature ; Brain ; Brain damage ; Brain injury ; Caspase ; Caspase-3 ; Cell death ; Cell Death - genetics ; Chickens ; Chromosomes ; Comet Assay ; Corticosterone ; Corticosterone - blood ; Cytokines ; Damage detection ; Deoxyribonucleic acid ; Disease ; DNA ; DNA Damage ; DNA methylation ; E coli ; Egg production ; Eggs ; Endotoxemia ; Escherichia coli ; Escherichia coli - physiology ; Escherichia coli Infections - veterinary ; Gene Expression ; Genes ; Genetic aspects ; Genomes ; Hepatocytes ; IL-1β ; Infections ; Inflammation ; Injection ; Investigations ; Kinases ; Liver ; MAP kinase ; Medicine and Health Sciences ; Mortality ; Physiological aspects ; Poultry ; Poultry Diseases - genetics ; Poultry Diseases - microbiology ; Protein kinase ; Proteins ; Shock ; Signal transduction ; Stress, Physiological - genetics ; Temperature ; Toxicity ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α</subject><ispartof>PloS one, 2016-06, Vol.11 (6), p.e0158314-e0158314</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Mehaisen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Mehaisen et al 2016 Mehaisen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c684t-d54ad8ce3cc3add5b6b21c4db1e04d9351c6b23bf80cd6088327484403c88bc53</citedby><cites>FETCH-LOGICAL-c684t-d54ad8ce3cc3add5b6b21c4db1e04d9351c6b23bf80cd6088327484403c88bc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4922552/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4922552/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27347679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mehaisen, Gamal M K</creatorcontrib><creatorcontrib>Eshak, Mariam G</creatorcontrib><creatorcontrib>El Sabry, M I</creatorcontrib><creatorcontrib>Abass, Ahmed O</creatorcontrib><title>Expression of Inflammatory and Cell Death Program Genes and Comet DNA Damage Assay Induced by Escherichia coli in Layer Hens</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Modern methods of industrial poultry and egg production systems involve stressful practices that stimulate Escherichia coli (E. coli) activity causing endotoxic shock. This investigation was conducted to evaluate the expression of pro-inflammatory cytokines and cell death program genes and DNA damage induced by E. coli in the brain and liver tissues of laying hens. A total of two hundred and ten H&N brown layer hens with 20 week age, were used in this research. First, preliminary experiments were designed (60 hens in total) to establish the optimal exposure dose of E. coli and to determine the nearest time of notable response to be used in the remainder studies of this research. At 35-wk of age, 150 hens were randomly assigned into 2 groups with 3 replicates of 25 birds each; the first group was injected in the brachial wing vein with 107 E. coli colony/hen, while the second group was injected with saline and served as a control. The body temperature and plasma corticosterone concentration were measured 3 hr after injection. Specimens of liver and brain were obtained from each group and the gene expression of p38 mitogen-activated protein kinase, interlukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), Bax, and caspase-3 genes were measured by quantitative real-time PCR. DNA damage in the brain and liver tissues were also measured by comet assay. Hens treated with E. coli showed significant (P<0.05) increase of body temperature and plasma corticosterone (42.6°C and 14.5 ng/ml, respectively) compared to the control group (41.1°C and 5.5 ng/ml, respectively). Additional remarkable over-inflammation gene expression of p38, IL-1β and TNF-α.genes were also detected in the brain (2.2-fold, 2.0-fold and 3.3-fold, respectively) and the liver (2.1-fold, 1.9-fold and 3.0-fold, respectively) tissues of the infected chickens. It is also important to note that hens injected with E. coli showed an increase in DNA damage in the brain and liver cells (P<0.05). These results were synchronized with activating cell death program since our data showed significant high expression of Bax gene by 2.8- and 2.7-fold and caspase-3 gene by 2.5- and 2.7-fold in the brain and liver tissues of infected chickens, respectively (P<0.05). In conclusion, the current study indicates that E. coli injection induces inflammatory physiological response and triggers cell death program in the brain and liver. Our results provide more understanding to endotoxic shock by E. coli in chickens at cellular level. Further studies are required to confirm if such responses are destructive or protective to set the means through which a chicken mounts a successful defense against avian pathogenic E. coli.</description><subject>Analysis</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>BAX gene</subject><subject>Bax protein</subject><subject>Bioassay</subject><subject>Bioassays</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Birds</subject><subject>Body Temperature</subject><subject>Brain</subject><subject>Brain damage</subject><subject>Brain injury</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Cell death</subject><subject>Cell Death - genetics</subject><subject>Chickens</subject><subject>Chromosomes</subject><subject>Comet Assay</subject><subject>Corticosterone</subject><subject>Corticosterone - blood</subject><subject>Cytokines</subject><subject>Damage detection</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>DNA methylation</subject><subject>E coli</subject><subject>Egg production</subject><subject>Eggs</subject><subject>Endotoxemia</subject><subject>Escherichia coli</subject><subject>Escherichia coli - physiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Hepatocytes</subject><subject>IL-1β</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Injection</subject><subject>Investigations</subject><subject>Kinases</subject><subject>Liver</subject><subject>MAP kinase</subject><subject>Medicine and Health Sciences</subject><subject>Mortality</subject><subject>Physiological aspects</subject><subject>Poultry</subject><subject>Poultry Diseases - genetics</subject><subject>Poultry Diseases - microbiology</subject><subject>Protein kinase</subject><subject>Proteins</subject><subject>Shock</subject><subject>Signal transduction</subject><subject>Stress, Physiological - genetics</subject><subject>Temperature</subject><subject>Toxicity</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkkGP0zAQhSMEYpeFf4DAEhLi0mLHjuNckKq27FaqgAOcrYk9aVMlcbETRCV-PC7NrlrEgZMtzzfPz-OXJC8ZnTKes_c7N_gOmunedTilLFOciUfJNSt4OpEp5Y_P9lfJsxB2lGZcSfk0uUpzLnKZF9fJr-XPvccQatcRV5FVVzXQttA7fyDQWTLHpiELhH5Lvni38dCSW-wwnIquxZ4sPs3IAlrYIJmFAIcoYgeDlpQHsgxmi7422xqIcU1N6o6s4YCe3GEXnidPKmgCvhjXm-Tbx-XX-d1k_fl2NZ-tJ0Yq0U9sJsAqg9wYDtZmpSxTZoQtGVJhC54xE094WSlqrKRK8TQXSgjKjVKlyfhN8vqku29c0OPggmZ5UaiMFbmKxOpEWAc7vfd1C_6gHdT6z4HzGw2-r02D2qTMogDJ0JSCy-iIMlqlFgF4btlR68N421C2aA12vYfmQvSy0tVbvXE_tCjSNMvSKPBuFPDu-4Ch120dTPwI6NAN0beiSnKa5fR_UCpoyqSM6Ju_0H8PYqQ2EN9ad5WLFs1RVM9EJgXnmRCRentGbRGafhtcM_QxRuESFCfQeBeCx-phDIzqY4zvTehjjPUY49j26nyED033ueW_ARur7m8</recordid><startdate>20160627</startdate><enddate>20160627</enddate><creator>Mehaisen, Gamal M K</creator><creator>Eshak, Mariam G</creator><creator>El Sabry, M I</creator><creator>Abass, Ahmed O</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160627</creationdate><title>Expression of Inflammatory and Cell Death Program Genes and Comet DNA Damage Assay Induced by Escherichia coli in Layer Hens</title><author>Mehaisen, Gamal M K ; Eshak, Mariam G ; El Sabry, M I ; Abass, Ahmed O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c684t-d54ad8ce3cc3add5b6b21c4db1e04d9351c6b23bf80cd6088327484403c88bc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>BAX gene</topic><topic>Bax protein</topic><topic>Bioassay</topic><topic>Bioassays</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Birds</topic><topic>Body Temperature</topic><topic>Brain</topic><topic>Brain damage</topic><topic>Brain injury</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Cell death</topic><topic>Cell Death - genetics</topic><topic>Chickens</topic><topic>Chromosomes</topic><topic>Comet Assay</topic><topic>Corticosterone</topic><topic>Corticosterone - blood</topic><topic>Cytokines</topic><topic>Damage detection</topic><topic>Deoxyribonucleic acid</topic><topic>Disease</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>DNA methylation</topic><topic>E coli</topic><topic>Egg production</topic><topic>Eggs</topic><topic>Endotoxemia</topic><topic>Escherichia coli</topic><topic>Escherichia coli - physiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Hepatocytes</topic><topic>IL-1β</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Injection</topic><topic>Investigations</topic><topic>Kinases</topic><topic>Liver</topic><topic>MAP kinase</topic><topic>Medicine and Health Sciences</topic><topic>Mortality</topic><topic>Physiological aspects</topic><topic>Poultry</topic><topic>Poultry Diseases - genetics</topic><topic>Poultry Diseases - microbiology</topic><topic>Protein kinase</topic><topic>Proteins</topic><topic>Shock</topic><topic>Signal transduction</topic><topic>Stress, Physiological - genetics</topic><topic>Temperature</topic><topic>Toxicity</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mehaisen, Gamal M K</creatorcontrib><creatorcontrib>Eshak, Mariam G</creatorcontrib><creatorcontrib>El Sabry, M I</creatorcontrib><creatorcontrib>Abass, Ahmed O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mehaisen, Gamal M K</au><au>Eshak, Mariam G</au><au>El Sabry, M I</au><au>Abass, Ahmed O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Inflammatory and Cell Death Program Genes and Comet DNA Damage Assay Induced by Escherichia coli in Layer Hens</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-06-27</date><risdate>2016</risdate><volume>11</volume><issue>6</issue><spage>e0158314</spage><epage>e0158314</epage><pages>e0158314-e0158314</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Modern methods of industrial poultry and egg production systems involve stressful practices that stimulate Escherichia coli (E. coli) activity causing endotoxic shock. This investigation was conducted to evaluate the expression of pro-inflammatory cytokines and cell death program genes and DNA damage induced by E. coli in the brain and liver tissues of laying hens. A total of two hundred and ten H&N brown layer hens with 20 week age, were used in this research. First, preliminary experiments were designed (60 hens in total) to establish the optimal exposure dose of E. coli and to determine the nearest time of notable response to be used in the remainder studies of this research. At 35-wk of age, 150 hens were randomly assigned into 2 groups with 3 replicates of 25 birds each; the first group was injected in the brachial wing vein with 107 E. coli colony/hen, while the second group was injected with saline and served as a control. The body temperature and plasma corticosterone concentration were measured 3 hr after injection. Specimens of liver and brain were obtained from each group and the gene expression of p38 mitogen-activated protein kinase, interlukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), Bax, and caspase-3 genes were measured by quantitative real-time PCR. DNA damage in the brain and liver tissues were also measured by comet assay. Hens treated with E. coli showed significant (P<0.05) increase of body temperature and plasma corticosterone (42.6°C and 14.5 ng/ml, respectively) compared to the control group (41.1°C and 5.5 ng/ml, respectively). Additional remarkable over-inflammation gene expression of p38, IL-1β and TNF-α.genes were also detected in the brain (2.2-fold, 2.0-fold and 3.3-fold, respectively) and the liver (2.1-fold, 1.9-fold and 3.0-fold, respectively) tissues of the infected chickens. It is also important to note that hens injected with E. coli showed an increase in DNA damage in the brain and liver cells (P<0.05). These results were synchronized with activating cell death program since our data showed significant high expression of Bax gene by 2.8- and 2.7-fold and caspase-3 gene by 2.5- and 2.7-fold in the brain and liver tissues of infected chickens, respectively (P<0.05). In conclusion, the current study indicates that E. coli injection induces inflammatory physiological response and triggers cell death program in the brain and liver. Our results provide more understanding to endotoxic shock by E. coli in chickens at cellular level. Further studies are required to confirm if such responses are destructive or protective to set the means through which a chicken mounts a successful defense against avian pathogenic E. coli.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27347679</pmid><doi>10.1371/journal.pone.0158314</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-06, Vol.11 (6), p.e0158314-e0158314 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1799851978 |
| source | Public Library of Science (PLoS) Journals Open Access; Open Access: PubMed Central; MEDLINE; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Analysis Animal tissues Animals Apoptosis Bacteria Bacterial infections BAX gene Bax protein Bioassay Bioassays Biology and Life Sciences Biomarkers Birds Body Temperature Brain Brain damage Brain injury Caspase Caspase-3 Cell death Cell Death - genetics Chickens Chromosomes Comet Assay Corticosterone Corticosterone - blood Cytokines Damage detection Deoxyribonucleic acid Disease DNA DNA Damage DNA methylation E coli Egg production Eggs Endotoxemia Escherichia coli Escherichia coli - physiology Escherichia coli Infections - veterinary Gene Expression Genes Genetic aspects Genomes Hepatocytes IL-1β Infections Inflammation Injection Investigations Kinases Liver MAP kinase Medicine and Health Sciences Mortality Physiological aspects Poultry Poultry Diseases - genetics Poultry Diseases - microbiology Protein kinase Proteins Shock Signal transduction Stress, Physiological - genetics Temperature Toxicity Tumor necrosis factor-TNF Tumor necrosis factor-α |
| title | Expression of Inflammatory and Cell Death Program Genes and Comet DNA Damage Assay Induced by Escherichia coli in Layer Hens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T19%3A29%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20Inflammatory%20and%20Cell%20Death%20Program%20Genes%20and%20Comet%20DNA%20Damage%20Assay%20Induced%20by%20Escherichia%20coli%20in%20Layer%20Hens&rft.jtitle=PloS%20one&rft.au=Mehaisen,%20Gamal%20M%20K&rft.date=2016-06-27&rft.volume=11&rft.issue=6&rft.spage=e0158314&rft.epage=e0158314&rft.pages=e0158314-e0158314&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0158314&rft_dat=%3Cgale_plos_%3EA456433544%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1799851978&rft_id=info:pmid/27347679&rft_galeid=A456433544&rft_doaj_id=oai_doaj_org_article_c21de4a61ecb4363ad010f2deaa37d18&rfr_iscdi=true |