Characterization of Mammalian ADAM2 and Its Absence from Human Sperm
The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-06, Vol.11 (6), p.e0158321-e0158321 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0158321 |
|---|---|
| container_issue | 6 |
| container_start_page | e0158321 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Choi, Heejin Jin, Sora Kwon, Jun Tae Kim, Jihye Jeong, Juri Kim, Jaehwan Jeon, Suyeon Park, Zee Yong Jung, Kang-Jin Park, Kwangsung Cho, Chunghee |
| description | The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other ADAMs form complexes that contribute to sperm-sperm adhesion, sperm-egg interactions, and the migration of sperm in the female reproductive tract. Here, we generated specific antibodies against mouse and human ADAM2, and investigated various features of ADAM2 in mice, monkeys and humans. We found that the cytoplasmic domain of ADAM2 might enable the differential association of this protein with other ADAMs in mice. Western blot analysis with the anti-human ADAM2 antibodies showed that ADAM2 is present in the testis and sperm of monkeys. Monkey ADAM2 was found to associate with chaperone proteins in testis. In humans, we identified ADAM2 as a 100-kDa protein in the testis, but failed to detect it in sperm. This is surprising given the results in mice and monkeys, but it is consistent with the failure of ADAM2 identification in the previous proteomic analyses of human sperm. These findings suggest that the reproductive functions of ADAM2 differ between humans and mice. Our protein analysis showed the presence of potential ADAM2 complexes involving yet-unknown proteins in human testis. Taken together, our results provide new information regarding the characteristics of ADAM2 in mammalian species, including humans. |
| doi_str_mv | 10.1371/journal.pone.0158321 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1799362764</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A456433612</galeid><doaj_id>oai_doaj_org_article_23f4cecaf1f446be94901a446ec10129</doaj_id><sourcerecordid>A456433612</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-d700e019ceb32a471d4cd804d36e758fea1ad68c22772af9cf1899d300a811863</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKv2s4NUtUBq7RpEgNurVPnuM2UxJ2dIODX49BuatAuJl_YOn7O6-PzkWUvKZlSruiHa9-HFurp1rc4JXSmOaOPsmNacDaRjPDHB-ej7FmM14TMuJbyaXbEFBeUC32cnS42EMB2GKo_0FW-zb3LL6BpoK6gzeen8wuWQ1vmyy7m81XE1mLugm_ys75JwNUWQ_M8e-Kgjvhiv59k3z9_-rY4m5xfflku5ucTq9ism5SKECS0sLjiDISipbClJqLkEtVMOwQKpdSWMaUYuMI6qoui5ISAplRLfpK93uluax_NPgHRUFUUXDIlRSKWO6L0cG22oWog_DYeKvPP4MPaQOgqW6Nh3AmLFhx1QsgVFqIgFNIRLSWUFUnr4_61ftVgabHtAtQj0fFNW23M2v80okgp1zwJvNsLBH_TY-xMU0WLdQ0t-j7FrYlWXEqlHoIOMRE-oG_-Q-9PxJ5aQ_pr1TqfQrSDqJmLWQK4pCxR03uotEpsKpsay1XJPnJ4P3JITIe_ujX0MZrl1deHs5c_xuzbA3aDUHeb6Ot-6Mg4BsUOtMHHGNDd1YMSM8zFbTbMMBdmPxfJ7dVhLe-cbgeB_wVP9AR5</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1799362764</pqid></control><display><type>article</type><title>Characterization of Mammalian ADAM2 and Its Absence from Human Sperm</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Choi, Heejin ; Jin, Sora ; Kwon, Jun Tae ; Kim, Jihye ; Jeong, Juri ; Kim, Jaehwan ; Jeon, Suyeon ; Park, Zee Yong ; Jung, Kang-Jin ; Park, Kwangsung ; Cho, Chunghee</creator><contributor>Gill, Andrew C.</contributor><creatorcontrib>Choi, Heejin ; Jin, Sora ; Kwon, Jun Tae ; Kim, Jihye ; Jeong, Juri ; Kim, Jaehwan ; Jeon, Suyeon ; Park, Zee Yong ; Jung, Kang-Jin ; Park, Kwangsung ; Cho, Chunghee ; Gill, Andrew C.</creatorcontrib><description>The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other ADAMs form complexes that contribute to sperm-sperm adhesion, sperm-egg interactions, and the migration of sperm in the female reproductive tract. Here, we generated specific antibodies against mouse and human ADAM2, and investigated various features of ADAM2 in mice, monkeys and humans. We found that the cytoplasmic domain of ADAM2 might enable the differential association of this protein with other ADAMs in mice. Western blot analysis with the anti-human ADAM2 antibodies showed that ADAM2 is present in the testis and sperm of monkeys. Monkey ADAM2 was found to associate with chaperone proteins in testis. In humans, we identified ADAM2 as a 100-kDa protein in the testis, but failed to detect it in sperm. This is surprising given the results in mice and monkeys, but it is consistent with the failure of ADAM2 identification in the previous proteomic analyses of human sperm. These findings suggest that the reproductive functions of ADAM2 differ between humans and mice. Our protein analysis showed the presence of potential ADAM2 complexes involving yet-unknown proteins in human testis. Taken together, our results provide new information regarding the characteristics of ADAM2 in mammalian species, including humans.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0158321</identifier><identifier>PMID: 27341348</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino Acid Sequence ; Amino acids ; Analysis ; Animals ; Antibodies ; Biology and Life Sciences ; Chemical properties ; Chromatography, Liquid ; Epidermal growth factor ; Failure analysis ; Fertilins - chemistry ; Fertilins - genetics ; Fertilins - metabolism ; Germ cells ; Humans ; Immunoglobulins ; Life sciences ; Macaca fascicularis ; Male ; Mammals ; Mammals - metabolism ; Medicine and Health Sciences ; Metalloproteinase ; Mice ; Monkeys ; Physiological aspects ; Protein Interaction Domains and Motifs ; Proteins ; Proteomics ; Reproduction ; Reproductive system ; Research and Analysis Methods ; Sperm ; Spermatozoa - metabolism ; Tandem Mass Spectrometry ; Testis - metabolism</subject><ispartof>PloS one, 2016-06, Vol.11 (6), p.e0158321-e0158321</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Choi et al 2016 Choi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-d700e019ceb32a471d4cd804d36e758fea1ad68c22772af9cf1899d300a811863</citedby><cites>FETCH-LOGICAL-c725t-d700e019ceb32a471d4cd804d36e758fea1ad68c22772af9cf1899d300a811863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920383/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4920383/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27341348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gill, Andrew C.</contributor><creatorcontrib>Choi, Heejin</creatorcontrib><creatorcontrib>Jin, Sora</creatorcontrib><creatorcontrib>Kwon, Jun Tae</creatorcontrib><creatorcontrib>Kim, Jihye</creatorcontrib><creatorcontrib>Jeong, Juri</creatorcontrib><creatorcontrib>Kim, Jaehwan</creatorcontrib><creatorcontrib>Jeon, Suyeon</creatorcontrib><creatorcontrib>Park, Zee Yong</creatorcontrib><creatorcontrib>Jung, Kang-Jin</creatorcontrib><creatorcontrib>Park, Kwangsung</creatorcontrib><creatorcontrib>Cho, Chunghee</creatorcontrib><title>Characterization of Mammalian ADAM2 and Its Absence from Human Sperm</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other ADAMs form complexes that contribute to sperm-sperm adhesion, sperm-egg interactions, and the migration of sperm in the female reproductive tract. Here, we generated specific antibodies against mouse and human ADAM2, and investigated various features of ADAM2 in mice, monkeys and humans. We found that the cytoplasmic domain of ADAM2 might enable the differential association of this protein with other ADAMs in mice. Western blot analysis with the anti-human ADAM2 antibodies showed that ADAM2 is present in the testis and sperm of monkeys. Monkey ADAM2 was found to associate with chaperone proteins in testis. In humans, we identified ADAM2 as a 100-kDa protein in the testis, but failed to detect it in sperm. This is surprising given the results in mice and monkeys, but it is consistent with the failure of ADAM2 identification in the previous proteomic analyses of human sperm. These findings suggest that the reproductive functions of ADAM2 differ between humans and mice. Our protein analysis showed the presence of potential ADAM2 complexes involving yet-unknown proteins in human testis. Taken together, our results provide new information regarding the characteristics of ADAM2 in mammalian species, including humans.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biology and Life Sciences</subject><subject>Chemical properties</subject><subject>Chromatography, Liquid</subject><subject>Epidermal growth factor</subject><subject>Failure analysis</subject><subject>Fertilins - chemistry</subject><subject>Fertilins - genetics</subject><subject>Fertilins - metabolism</subject><subject>Germ cells</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Life sciences</subject><subject>Macaca fascicularis</subject><subject>Male</subject><subject>Mammals</subject><subject>Mammals - metabolism</subject><subject>Medicine and Health Sciences</subject><subject>Metalloproteinase</subject><subject>Mice</subject><subject>Monkeys</subject><subject>Physiological aspects</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Reproduction</subject><subject>Reproductive system</subject><subject>Research and Analysis Methods</subject><subject>Sperm</subject><subject>Spermatozoa - metabolism</subject><subject>Tandem Mass Spectrometry</subject><subject>Testis - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKv2s4NUtUBq7RpEgNurVPnuM2UxJ2dIODX49BuatAuJl_YOn7O6-PzkWUvKZlSruiHa9-HFurp1rc4JXSmOaOPsmNacDaRjPDHB-ej7FmM14TMuJbyaXbEFBeUC32cnS42EMB2GKo_0FW-zb3LL6BpoK6gzeen8wuWQ1vmyy7m81XE1mLugm_ys75JwNUWQ_M8e-Kgjvhiv59k3z9_-rY4m5xfflku5ucTq9ism5SKECS0sLjiDISipbClJqLkEtVMOwQKpdSWMaUYuMI6qoui5ISAplRLfpK93uluax_NPgHRUFUUXDIlRSKWO6L0cG22oWog_DYeKvPP4MPaQOgqW6Nh3AmLFhx1QsgVFqIgFNIRLSWUFUnr4_61ftVgabHtAtQj0fFNW23M2v80okgp1zwJvNsLBH_TY-xMU0WLdQ0t-j7FrYlWXEqlHoIOMRE-oG_-Q-9PxJ5aQ_pr1TqfQrSDqJmLWQK4pCxR03uotEpsKpsay1XJPnJ4P3JITIe_ujX0MZrl1deHs5c_xuzbA3aDUHeb6Ot-6Mg4BsUOtMHHGNDd1YMSM8zFbTbMMBdmPxfJ7dVhLe-cbgeB_wVP9AR5</recordid><startdate>20160624</startdate><enddate>20160624</enddate><creator>Choi, Heejin</creator><creator>Jin, Sora</creator><creator>Kwon, Jun Tae</creator><creator>Kim, Jihye</creator><creator>Jeong, Juri</creator><creator>Kim, Jaehwan</creator><creator>Jeon, Suyeon</creator><creator>Park, Zee Yong</creator><creator>Jung, Kang-Jin</creator><creator>Park, Kwangsung</creator><creator>Cho, Chunghee</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160624</creationdate><title>Characterization of Mammalian ADAM2 and Its Absence from Human Sperm</title><author>Choi, Heejin ; Jin, Sora ; Kwon, Jun Tae ; Kim, Jihye ; Jeong, Juri ; Kim, Jaehwan ; Jeon, Suyeon ; Park, Zee Yong ; Jung, Kang-Jin ; Park, Kwangsung ; Cho, Chunghee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-d700e019ceb32a471d4cd804d36e758fea1ad68c22772af9cf1899d300a811863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Biology and Life Sciences</topic><topic>Chemical properties</topic><topic>Chromatography, Liquid</topic><topic>Epidermal growth factor</topic><topic>Failure analysis</topic><topic>Fertilins - chemistry</topic><topic>Fertilins - genetics</topic><topic>Fertilins - metabolism</topic><topic>Germ cells</topic><topic>Humans</topic><topic>Immunoglobulins</topic><topic>Life sciences</topic><topic>Macaca fascicularis</topic><topic>Male</topic><topic>Mammals</topic><topic>Mammals - metabolism</topic><topic>Medicine and Health Sciences</topic><topic>Metalloproteinase</topic><topic>Mice</topic><topic>Monkeys</topic><topic>Physiological aspects</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Reproduction</topic><topic>Reproductive system</topic><topic>Research and Analysis Methods</topic><topic>Sperm</topic><topic>Spermatozoa - metabolism</topic><topic>Tandem Mass Spectrometry</topic><topic>Testis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Heejin</creatorcontrib><creatorcontrib>Jin, Sora</creatorcontrib><creatorcontrib>Kwon, Jun Tae</creatorcontrib><creatorcontrib>Kim, Jihye</creatorcontrib><creatorcontrib>Jeong, Juri</creatorcontrib><creatorcontrib>Kim, Jaehwan</creatorcontrib><creatorcontrib>Jeon, Suyeon</creatorcontrib><creatorcontrib>Park, Zee Yong</creatorcontrib><creatorcontrib>Jung, Kang-Jin</creatorcontrib><creatorcontrib>Park, Kwangsung</creatorcontrib><creatorcontrib>Cho, Chunghee</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Heejin</au><au>Jin, Sora</au><au>Kwon, Jun Tae</au><au>Kim, Jihye</au><au>Jeong, Juri</au><au>Kim, Jaehwan</au><au>Jeon, Suyeon</au><au>Park, Zee Yong</au><au>Jung, Kang-Jin</au><au>Park, Kwangsung</au><au>Cho, Chunghee</au><au>Gill, Andrew C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Mammalian ADAM2 and Its Absence from Human Sperm</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-06-24</date><risdate>2016</risdate><volume>11</volume><issue>6</issue><spage>e0158321</spage><epage>e0158321</epage><pages>e0158321-e0158321</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other ADAMs form complexes that contribute to sperm-sperm adhesion, sperm-egg interactions, and the migration of sperm in the female reproductive tract. Here, we generated specific antibodies against mouse and human ADAM2, and investigated various features of ADAM2 in mice, monkeys and humans. We found that the cytoplasmic domain of ADAM2 might enable the differential association of this protein with other ADAMs in mice. Western blot analysis with the anti-human ADAM2 antibodies showed that ADAM2 is present in the testis and sperm of monkeys. Monkey ADAM2 was found to associate with chaperone proteins in testis. In humans, we identified ADAM2 as a 100-kDa protein in the testis, but failed to detect it in sperm. This is surprising given the results in mice and monkeys, but it is consistent with the failure of ADAM2 identification in the previous proteomic analyses of human sperm. These findings suggest that the reproductive functions of ADAM2 differ between humans and mice. Our protein analysis showed the presence of potential ADAM2 complexes involving yet-unknown proteins in human testis. Taken together, our results provide new information regarding the characteristics of ADAM2 in mammalian species, including humans.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27341348</pmid><doi>10.1371/journal.pone.0158321</doi><tpages>e0158321</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-06, Vol.11 (6), p.e0158321-e0158321 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1799362764 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Amino acids Analysis Animals Antibodies Biology and Life Sciences Chemical properties Chromatography, Liquid Epidermal growth factor Failure analysis Fertilins - chemistry Fertilins - genetics Fertilins - metabolism Germ cells Humans Immunoglobulins Life sciences Macaca fascicularis Male Mammals Mammals - metabolism Medicine and Health Sciences Metalloproteinase Mice Monkeys Physiological aspects Protein Interaction Domains and Motifs Proteins Proteomics Reproduction Reproductive system Research and Analysis Methods Sperm Spermatozoa - metabolism Tandem Mass Spectrometry Testis - metabolism |
| title | Characterization of Mammalian ADAM2 and Its Absence from Human Sperm |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T01%3A57%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20Mammalian%20ADAM2%20and%20Its%20Absence%20from%20Human%20Sperm&rft.jtitle=PloS%20one&rft.au=Choi,%20Heejin&rft.date=2016-06-24&rft.volume=11&rft.issue=6&rft.spage=e0158321&rft.epage=e0158321&rft.pages=e0158321-e0158321&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0158321&rft_dat=%3Cgale_plos_%3EA456433612%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1799362764&rft_id=info:pmid/27341348&rft_galeid=A456433612&rft_doaj_id=oai_doaj_org_article_23f4cecaf1f446be94901a446ec10129&rfr_iscdi=true |