Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)
Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and wat...
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| description | Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together with histological characterization. Metabolite profiling showed relatively higher levels of glutamine, arginine and lysine in embryogenic callus than in dry and watery calli, while shoot base tissue showed an intermediate level of primary metabolites. For the five secondary metabolites analyzed (ie. panduratin, pinocembrin, pinostrobin, cardamonin and alpinetin), shoot base had the highest concentrations, followed by watery, dry and embryogenic calli. Furthermore, intracellular auxin levels were found to decrease from dry to watery calli, followed by shoot base and finally embryogenic calli. Our morphological observations showed the presence of fibrils on the cell surface of embryogenic callus while diphenylboric acid 2-aminoethylester staining indicated the presence of flavonoids in both dry and embryogenic calli. Periodic acid-Schiff staining showed that shoot base and dry and embryogenic calli contained starch reserves while none were found in watery callus. This study identified several primary metabolites that could be used as markers of embryogenic cells in B. rotunda, while secondary metabolite analysis indicated that biosynthesis pathways of these important metabolites may not be active in callus and embryogenic tissue. |
| doi_str_mv | 10.1371/journal.pone.0156714 |
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In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together with histological characterization. Metabolite profiling showed relatively higher levels of glutamine, arginine and lysine in embryogenic callus than in dry and watery calli, while shoot base tissue showed an intermediate level of primary metabolites. For the five secondary metabolites analyzed (ie. panduratin, pinocembrin, pinostrobin, cardamonin and alpinetin), shoot base had the highest concentrations, followed by watery, dry and embryogenic calli. Furthermore, intracellular auxin levels were found to decrease from dry to watery calli, followed by shoot base and finally embryogenic calli. Our morphological observations showed the presence of fibrils on the cell surface of embryogenic callus while diphenylboric acid 2-aminoethylester staining indicated the presence of flavonoids in both dry and embryogenic calli. Periodic acid-Schiff staining showed that shoot base and dry and embryogenic calli contained starch reserves while none were found in watery callus. This study identified several primary metabolites that could be used as markers of embryogenic cells in B. rotunda, while secondary metabolite analysis indicated that biosynthesis pathways of these important metabolites may not be active in callus and embryogenic tissue.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0156714</identifier><identifier>PMID: 27258536</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Amino acids ; Amino Acids - chemistry ; Arginine ; Biology and Life Sciences ; Biosynthesis ; Boesenbergia rotunda ; Boron Compounds - chemistry ; Callus ; Cell culture ; Cell surface ; Chromatography ; Chromatography, High Pressure Liquid ; Culture Media ; Esters - chemistry ; Fibrils ; Flavonoids ; Ginger ; Glutamine ; Hormones ; Indoleacetic Acids - chemistry ; Influence ; Liquid chromatography ; Lymphocytes B ; Lysine ; Mass Spectrometry ; Mass spectroscopy ; Metabolites ; Metabolome ; Physical Sciences ; Physiological aspects ; Plant Shoots - metabolism ; Principal Component Analysis ; Research and Analysis Methods ; Secondary metabolites ; Staining ; Starch ; Tissue culture ; Tissue Culture Techniques ; Zingiber officinale - embryology ; Zingiber officinale - metabolism ; Zingiberaceae</subject><ispartof>PloS one, 2016-06, Vol.11 (6), p.e0156714</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Ng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Ng et al 2016 Ng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-d69991420eadaf4c36546c67a8846d44e6c7dcbe978679451a0e0b231350b3ca3</citedby><cites>FETCH-LOGICAL-c725t-d69991420eadaf4c36546c67a8846d44e6c7dcbe978679451a0e0b231350b3ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892522/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4892522/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27258536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mudiam, Mohana Krishna Reddy</contributor><creatorcontrib>Ng, Theresa Lee Mei</creatorcontrib><creatorcontrib>Karim, Rezaul</creatorcontrib><creatorcontrib>Tan, Yew Seong</creatorcontrib><creatorcontrib>Teh, Huey Fang</creatorcontrib><creatorcontrib>Danial, Asma Dazni</creatorcontrib><creatorcontrib>Ho, Li Sim</creatorcontrib><creatorcontrib>Khalid, Norzulaani</creatorcontrib><creatorcontrib>Appleton, David Ross</creatorcontrib><creatorcontrib>Harikrishna, Jennifer Ann</creatorcontrib><title>Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. 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Our morphological observations showed the presence of fibrils on the cell surface of embryogenic callus while diphenylboric acid 2-aminoethylester staining indicated the presence of flavonoids in both dry and embryogenic calli. Periodic acid-Schiff staining showed that shoot base and dry and embryogenic calli contained starch reserves while none were found in watery callus. This study identified several primary metabolites that could be used as markers of embryogenic cells in B. rotunda, while secondary metabolite analysis indicated that biosynthesis pathways of these important metabolites may not be active in callus and embryogenic tissue.</description><subject>Acids</subject><subject>Amino acids</subject><subject>Amino Acids - chemistry</subject><subject>Arginine</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Boesenbergia rotunda</subject><subject>Boron Compounds - chemistry</subject><subject>Callus</subject><subject>Cell culture</subject><subject>Cell surface</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Culture Media</subject><subject>Esters - chemistry</subject><subject>Fibrils</subject><subject>Flavonoids</subject><subject>Ginger</subject><subject>Glutamine</subject><subject>Hormones</subject><subject>Indoleacetic Acids - chemistry</subject><subject>Influence</subject><subject>Liquid chromatography</subject><subject>Lymphocytes B</subject><subject>Lysine</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Metabolome</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Plant Shoots - metabolism</subject><subject>Principal Component Analysis</subject><subject>Research and Analysis Methods</subject><subject>Secondary metabolites</subject><subject>Staining</subject><subject>Starch</subject><subject>Tissue culture</subject><subject>Tissue Culture Techniques</subject><subject>Zingiber officinale - embryology</subject><subject>Zingiber officinale - metabolism</subject><subject>Zingiberaceae</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYqPwDxBEQkLbRYsdfyS5mVSqbVQaDDHg1nKck9RVYhc7QeyeH47bZlODJoF8Yev4Oa-Pz0cUvcRohkmK361t74xsZhtrYIYw4ymmj6JjnJNkyhNEHh-cj6Jn3q8RYiTj_Gl0lKQJyxjhx9HveauNjedKl7E0ZXwDyppSutv4I3SysI3uIP7sbNmrTlsTaxOft4W7tTUYrXYun6yZHtoWsml6H9sqvtCmBues7eLL3TE-eW_BgynA1VrGznZ9eOz0efSkko2HF8M-ib5dnH9dfJheXV8uF_OrqQrxdtOS53mOaYJAlrKiinBGueKpzDLKS0qBq7RUBeRpxtOcMiwRoCIhmDBUECXJJHq919001oshgV7gNCcpIyRsk2i5J0or12LjdBtSIazUYmewrhbSdVo1ICgAykqa5QXPKc5RxillpOI4kVgSRYLW2fBaX7RQKjCdk81IdHxj9ErU9qcImglLkiBwMgg4-6MH34lWewVNIw3YPsSdoSxNCEL432jIB02T0BABffMX-nAiBqqW4a_aVDaEqLaiYk5ZqAFjZEvNHqDCKqHVoZGg0sE-cjgdOQSmg19dLXvvxfLmy_-z19_H7NsDdgWy6VbeNv22Z_0YpHtQOeu9g-q-HhiJ7VzdZUNs50oMcxXcXh3W8t7pbpDIH9mAHEc</recordid><startdate>20160603</startdate><enddate>20160603</enddate><creator>Ng, Theresa Lee Mei</creator><creator>Karim, Rezaul</creator><creator>Tan, Yew Seong</creator><creator>Teh, Huey Fang</creator><creator>Danial, Asma Dazni</creator><creator>Ho, Li Sim</creator><creator>Khalid, Norzulaani</creator><creator>Appleton, David Ross</creator><creator>Harikrishna, Jennifer Ann</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160603</creationdate><title>Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)</title><author>Ng, Theresa Lee Mei ; Karim, Rezaul ; Tan, Yew Seong ; Teh, Huey Fang ; Danial, Asma Dazni ; Ho, Li Sim ; Khalid, Norzulaani ; Appleton, David Ross ; Harikrishna, Jennifer Ann</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-d69991420eadaf4c36546c67a8846d44e6c7dcbe978679451a0e0b231350b3ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acids</topic><topic>Amino acids</topic><topic>Amino Acids - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, Theresa Lee Mei</au><au>Karim, Rezaul</au><au>Tan, Yew Seong</au><au>Teh, Huey Fang</au><au>Danial, Asma Dazni</au><au>Ho, Li Sim</au><au>Khalid, Norzulaani</au><au>Appleton, David Ross</au><au>Harikrishna, Jennifer Ann</au><au>Mudiam, Mohana Krishna Reddy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-06-03</date><risdate>2016</risdate><volume>11</volume><issue>6</issue><spage>e0156714</spage><pages>e0156714-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together with histological characterization. Metabolite profiling showed relatively higher levels of glutamine, arginine and lysine in embryogenic callus than in dry and watery calli, while shoot base tissue showed an intermediate level of primary metabolites. For the five secondary metabolites analyzed (ie. panduratin, pinocembrin, pinostrobin, cardamonin and alpinetin), shoot base had the highest concentrations, followed by watery, dry and embryogenic calli. Furthermore, intracellular auxin levels were found to decrease from dry to watery calli, followed by shoot base and finally embryogenic calli. Our morphological observations showed the presence of fibrils on the cell surface of embryogenic callus while diphenylboric acid 2-aminoethylester staining indicated the presence of flavonoids in both dry and embryogenic calli. Periodic acid-Schiff staining showed that shoot base and dry and embryogenic calli contained starch reserves while none were found in watery callus. This study identified several primary metabolites that could be used as markers of embryogenic cells in B. rotunda, while secondary metabolite analysis indicated that biosynthesis pathways of these important metabolites may not be active in callus and embryogenic tissue.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27258536</pmid><doi>10.1371/journal.pone.0156714</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-06, Vol.11 (6), p.e0156714 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1793753379 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Amino acids Amino Acids - chemistry Arginine Biology and Life Sciences Biosynthesis Boesenbergia rotunda Boron Compounds - chemistry Callus Cell culture Cell surface Chromatography Chromatography, High Pressure Liquid Culture Media Esters - chemistry Fibrils Flavonoids Ginger Glutamine Hormones Indoleacetic Acids - chemistry Influence Liquid chromatography Lymphocytes B Lysine Mass Spectrometry Mass spectroscopy Metabolites Metabolome Physical Sciences Physiological aspects Plant Shoots - metabolism Principal Component Analysis Research and Analysis Methods Secondary metabolites Staining Starch Tissue culture Tissue Culture Techniques Zingiber officinale - embryology Zingiber officinale - metabolism Zingiberaceae |
| title | Amino Acid and Secondary Metabolite Production in Embryogenic and Non-Embryogenic Callus of Fingerroot Ginger (Boesenbergia rotunda) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T21%3A30%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Amino%20Acid%20and%20Secondary%20Metabolite%20Production%20in%20Embryogenic%20and%20Non-Embryogenic%20Callus%20of%20Fingerroot%20Ginger%20(Boesenbergia%20rotunda)&rft.jtitle=PloS%20one&rft.au=Ng,%20Theresa%20Lee%20Mei&rft.date=2016-06-03&rft.volume=11&rft.issue=6&rft.spage=e0156714&rft.pages=e0156714-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0156714&rft_dat=%3Cgale_plos_%3EA454205539%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1793753379&rft_id=info:pmid/27258536&rft_galeid=A454205539&rft_doaj_id=oai_doaj_org_article_4ee08d489b694190864453f612a1a3c3&rfr_iscdi=true |