Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides

Lipopolysaccharide (LPS), the major constituent of the outer membrane of Gram-negative bacteria, can trigger severe inflammatory responses during bacterial infections, possibly leading to septic shock. One approach to combatting endotoxic shock is to neutralize the most conserved part and major medi...

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Veröffentlicht in:	PloS one 2016-06, Vol.11 (6), p.e0156530-e0156530
Hauptverfasser:	Martínez-Sernández, Victoria, Orbegozo-Medina, Ricardo A, Romarís, Fernanda, Paniagua, Esperanza, Ubeira, Florencio M
Format:	Artikel
Sprache:	eng
Schlagworte:	Avidin Bacteria Binding Biological activity Biology and Life Sciences Casein Detergents E coli Endotoxemia Engineering and Technology Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Escherichia coli Genetic aspects Gram-negative bacteria Inflammation Lipid A Lipopolysaccharides Lipopolysaccharides - metabolism Macrophages Medicine and Health Sciences Methods Peptides Peptides - metabolism pH effects Physical Sciences Physiological aspects Polymyxin B Protein Binding Proteins Proteins - metabolism Research and Analysis Methods Septic shock Serotypes Tumor necrosis factor-α
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