Investigating Oral Microbiome Profiles in Children with Cleft Lip and Palate for Prognosis of Alveolar Bone Grafting
In this study, we sought to investigate the oral microbiota structure of children with cleft lip and palate (CLP) and explore the pre-operative oral bacterial composition related to the prognosis of alveolar bone grafting. In total, 28 patients (19 boys, 9 girls) with CLP who were scheduled to under...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-05, Vol.11 (5), p.e0155683-e0155683 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0155683 |
|---|---|
| container_issue | 5 |
| container_start_page | e0155683 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Liu, Luwei Zhang, Qian Lin, Jiuxiang Ma, Lian Zhou, Zhibo He, Xuesong Jia, Yilin Chen, Feng |
| description | In this study, we sought to investigate the oral microbiota structure of children with cleft lip and palate (CLP) and explore the pre-operative oral bacterial composition related to the prognosis of alveolar bone grafting. In total, 28 patients (19 boys, 9 girls) with CLP who were scheduled to undergo alveolar bone grafting for the first time were recruited. According to the clinical examination of operative sites at the third month after the operation, the individuals were divided into a non-inflammation group (n = 15) and an inflammation group (n = 13). In all, 56 unstimulated saliva samples were collected before and after the operation. The v3-v4 hypervariable regions of the 16S rRNA gene were sequenced using an Illumina MiSeq sequencing platform. Based on the beta diversity of the operational taxonomic units (OTUs) in the inflammation and non-inflammation samples, the microbial variation in the oral cavity differed significantly between the two groups before and after the operation (P < 0.05). Analysis of the relative abundances of pre-operative OTUs revealed 26 OTUs with a relative abundance higher than 0.01%, reflecting a significant difference of the relative abundance between groups (P < 0.05). According to a principal component analysis of the pre-operative samples, the inflammation-related OTUs included Tannerella sp., Porphyromonas sp., Gemella sp., Moraxella sp., Prevotella nigrescens, and Prevotella intermedia, most of which were enriched in the inflammation group and showed a significant positive correlation. A cross-validated random forest model based on the 26 different OTUs before the operation was able to fit the post-operative status of grafted sites and yielded a good classification result. The sensitivity and specificity of this classified model were 76.9% and 86.7%, respectively. These findings show that the oral microbiota profile before alveolar bone grafting may be related to the risk of post-operative inflammation at grafted sites. |
| doi_str_mv | 10.1371/journal.pone.0155683 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1789766306</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A453360307</galeid><doaj_id>oai_doaj_org_article_55802a0eefd64c228efcc6a2f71f50fa</doaj_id><sourcerecordid>A453360307</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-6c523ff93ebb330df67e63866bcc45f376a0132b03dc278282e2c0c2284326cc3</originalsourceid><addsrcrecordid>eNqNk02P0zAQhiMEYpeFf4DAEhKCQ4s_Eie5IJUKlkpFXfF1tRzHTr1y7WI7Bf49Ds2uGrQH5EMs53nf8cx4suwpgnNESvTm2vXecjPfOyvnEBUFrci97BzVBM8ohuT-yf4sexTCNYQFqSh9mJ3hEtWI1PA8iyt7kCHqjkdtO7Dx3IBPWnjXaLeT4Mo7pY0MQFuw3GrTemnBTx23YGmkimCt94DbFlxxw6MEyvlB0lkXdABOgYU5SGe4B-_SLcGl52oI8zh7oLgJ8sn4vci-fXj_dflxtt5crpaL9UzQGscZFQUmStVENg0hsFW0lHTIoBEiLxQpKYeI4AaSVuCywhWWWECBcZUTTIUgF9nzo-_euMDGggWGyqouKSWQJmJ1JFrHr9ne6x33v5njmv09cL5j3EctjGRFUUHMoZSqpfkQRCohKMeqRKqAiievt2O0vtnJVkgbUzUnptM_Vm9Z5w4sr0pU5GUyeDUaePejT11hOx2ENIZb6frh3jXMC0JLlNAX_6B3ZzdSHU8JaKtciisGU7ZIRoRCAoew8zuotFq50yL1bXgBU8HriSAxUf6KHe9DYKsvn_-f3Xyfsi9P2K3kJm6DM33UzoYpmB_B9E5D8FLdFhlBNgzHTTXYMBxsHI4ke3baoFvRzTSQP1nXCZA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1789766306</pqid></control><display><type>article</type><title>Investigating Oral Microbiome Profiles in Children with Cleft Lip and Palate for Prognosis of Alveolar Bone Grafting</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Liu, Luwei ; Zhang, Qian ; Lin, Jiuxiang ; Ma, Lian ; Zhou, Zhibo ; He, Xuesong ; Jia, Yilin ; Chen, Feng</creator><creatorcontrib>Liu, Luwei ; Zhang, Qian ; Lin, Jiuxiang ; Ma, Lian ; Zhou, Zhibo ; He, Xuesong ; Jia, Yilin ; Chen, Feng</creatorcontrib><description>In this study, we sought to investigate the oral microbiota structure of children with cleft lip and palate (CLP) and explore the pre-operative oral bacterial composition related to the prognosis of alveolar bone grafting. In total, 28 patients (19 boys, 9 girls) with CLP who were scheduled to undergo alveolar bone grafting for the first time were recruited. According to the clinical examination of operative sites at the third month after the operation, the individuals were divided into a non-inflammation group (n = 15) and an inflammation group (n = 13). In all, 56 unstimulated saliva samples were collected before and after the operation. The v3-v4 hypervariable regions of the 16S rRNA gene were sequenced using an Illumina MiSeq sequencing platform. Based on the beta diversity of the operational taxonomic units (OTUs) in the inflammation and non-inflammation samples, the microbial variation in the oral cavity differed significantly between the two groups before and after the operation (P < 0.05). Analysis of the relative abundances of pre-operative OTUs revealed 26 OTUs with a relative abundance higher than 0.01%, reflecting a significant difference of the relative abundance between groups (P < 0.05). According to a principal component analysis of the pre-operative samples, the inflammation-related OTUs included Tannerella sp., Porphyromonas sp., Gemella sp., Moraxella sp., Prevotella nigrescens, and Prevotella intermedia, most of which were enriched in the inflammation group and showed a significant positive correlation. A cross-validated random forest model based on the 26 different OTUs before the operation was able to fit the post-operative status of grafted sites and yielded a good classification result. The sensitivity and specificity of this classified model were 76.9% and 86.7%, respectively. These findings show that the oral microbiota profile before alveolar bone grafting may be related to the risk of post-operative inflammation at grafted sites.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0155683</identifier><identifier>PMID: 27191390</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abundance ; Adolescent ; Alveolar bone ; Alveolar Bone Grafting ; Analysis ; Antibiotics ; Bacteria ; Biodiversity ; Biofilms ; Biology and Life Sciences ; Bone composition ; Bone grafts ; Care and treatment ; Child ; Children ; Cleft lip ; Cleft Lip - microbiology ; Cleft Lip - surgery ; Cleft lip/palate ; Cleft Palate - microbiology ; Cleft Palate - surgery ; Ecology and Environmental Sciences ; Female ; Fistula ; Gene expression ; Gene sequencing ; Girls ; Grafting ; Hospitals ; Humans ; Infections ; Inflammation ; Investigations ; Laboratories ; Male ; Maxillofacial surgery ; Medical prognosis ; Medicine and Health Sciences ; Metagenome ; Metagenomics - methods ; Microbiomes ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Mouth - microbiology ; Oral cavity ; Orthodontics ; Pathogenesis ; Patient outcomes ; Physical growth ; Physiological aspects ; Porphyromonas gingivalis ; Preoperative Period ; Principal components analysis ; Prognosis ; Relative abundance ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Saliva ; Saliva - microbiology ; Skin & tissue grafts ; Studies ; Substitute bone ; Surgery</subject><ispartof>PloS one, 2016-05, Vol.11 (5), p.e0155683-e0155683</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Liu et al 2016 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6c523ff93ebb330df67e63866bcc45f376a0132b03dc278282e2c0c2284326cc3</citedby><cites>FETCH-LOGICAL-c692t-6c523ff93ebb330df67e63866bcc45f376a0132b03dc278282e2c0c2284326cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871547/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871547/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27191390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Luwei</creatorcontrib><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Lin, Jiuxiang</creatorcontrib><creatorcontrib>Ma, Lian</creatorcontrib><creatorcontrib>Zhou, Zhibo</creatorcontrib><creatorcontrib>He, Xuesong</creatorcontrib><creatorcontrib>Jia, Yilin</creatorcontrib><creatorcontrib>Chen, Feng</creatorcontrib><title>Investigating Oral Microbiome Profiles in Children with Cleft Lip and Palate for Prognosis of Alveolar Bone Grafting</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In this study, we sought to investigate the oral microbiota structure of children with cleft lip and palate (CLP) and explore the pre-operative oral bacterial composition related to the prognosis of alveolar bone grafting. In total, 28 patients (19 boys, 9 girls) with CLP who were scheduled to undergo alveolar bone grafting for the first time were recruited. According to the clinical examination of operative sites at the third month after the operation, the individuals were divided into a non-inflammation group (n = 15) and an inflammation group (n = 13). In all, 56 unstimulated saliva samples were collected before and after the operation. The v3-v4 hypervariable regions of the 16S rRNA gene were sequenced using an Illumina MiSeq sequencing platform. Based on the beta diversity of the operational taxonomic units (OTUs) in the inflammation and non-inflammation samples, the microbial variation in the oral cavity differed significantly between the two groups before and after the operation (P < 0.05). Analysis of the relative abundances of pre-operative OTUs revealed 26 OTUs with a relative abundance higher than 0.01%, reflecting a significant difference of the relative abundance between groups (P < 0.05). According to a principal component analysis of the pre-operative samples, the inflammation-related OTUs included Tannerella sp., Porphyromonas sp., Gemella sp., Moraxella sp., Prevotella nigrescens, and Prevotella intermedia, most of which were enriched in the inflammation group and showed a significant positive correlation. A cross-validated random forest model based on the 26 different OTUs before the operation was able to fit the post-operative status of grafted sites and yielded a good classification result. The sensitivity and specificity of this classified model were 76.9% and 86.7%, respectively. These findings show that the oral microbiota profile before alveolar bone grafting may be related to the risk of post-operative inflammation at grafted sites.</description><subject>Abundance</subject><subject>Adolescent</subject><subject>Alveolar bone</subject><subject>Alveolar Bone Grafting</subject><subject>Analysis</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Biodiversity</subject><subject>Biofilms</subject><subject>Biology and Life Sciences</subject><subject>Bone composition</subject><subject>Bone grafts</subject><subject>Care and treatment</subject><subject>Child</subject><subject>Children</subject><subject>Cleft lip</subject><subject>Cleft Lip - microbiology</subject><subject>Cleft Lip - surgery</subject><subject>Cleft lip/palate</subject><subject>Cleft Palate - microbiology</subject><subject>Cleft Palate - surgery</subject><subject>Ecology and Environmental Sciences</subject><subject>Female</subject><subject>Fistula</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Girls</subject><subject>Grafting</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Investigations</subject><subject>Laboratories</subject><subject>Male</subject><subject>Maxillofacial surgery</subject><subject>Medical prognosis</subject><subject>Medicine and Health Sciences</subject><subject>Metagenome</subject><subject>Metagenomics - methods</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microorganisms</subject><subject>Mouth - microbiology</subject><subject>Oral cavity</subject><subject>Orthodontics</subject><subject>Pathogenesis</subject><subject>Patient outcomes</subject><subject>Physical growth</subject><subject>Physiological aspects</subject><subject>Porphyromonas gingivalis</subject><subject>Preoperative Period</subject><subject>Principal components analysis</subject><subject>Prognosis</subject><subject>Relative abundance</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Saliva</subject><subject>Saliva - microbiology</subject><subject>Skin & tissue grafts</subject><subject>Studies</subject><subject>Substitute bone</subject><subject>Surgery</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk02P0zAQhiMEYpeFf4DAEhKCQ4s_Eie5IJUKlkpFXfF1tRzHTr1y7WI7Bf49Ds2uGrQH5EMs53nf8cx4suwpgnNESvTm2vXecjPfOyvnEBUFrci97BzVBM8ohuT-yf4sexTCNYQFqSh9mJ3hEtWI1PA8iyt7kCHqjkdtO7Dx3IBPWnjXaLeT4Mo7pY0MQFuw3GrTemnBTx23YGmkimCt94DbFlxxw6MEyvlB0lkXdABOgYU5SGe4B-_SLcGl52oI8zh7oLgJ8sn4vci-fXj_dflxtt5crpaL9UzQGscZFQUmStVENg0hsFW0lHTIoBEiLxQpKYeI4AaSVuCywhWWWECBcZUTTIUgF9nzo-_euMDGggWGyqouKSWQJmJ1JFrHr9ne6x33v5njmv09cL5j3EctjGRFUUHMoZSqpfkQRCohKMeqRKqAiievt2O0vtnJVkgbUzUnptM_Vm9Z5w4sr0pU5GUyeDUaePejT11hOx2ENIZb6frh3jXMC0JLlNAX_6B3ZzdSHU8JaKtciisGU7ZIRoRCAoew8zuotFq50yL1bXgBU8HriSAxUf6KHe9DYKsvn_-f3Xyfsi9P2K3kJm6DM33UzoYpmB_B9E5D8FLdFhlBNgzHTTXYMBxsHI4ke3baoFvRzTSQP1nXCZA</recordid><startdate>20160518</startdate><enddate>20160518</enddate><creator>Liu, Luwei</creator><creator>Zhang, Qian</creator><creator>Lin, Jiuxiang</creator><creator>Ma, Lian</creator><creator>Zhou, Zhibo</creator><creator>He, Xuesong</creator><creator>Jia, Yilin</creator><creator>Chen, Feng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160518</creationdate><title>Investigating Oral Microbiome Profiles in Children with Cleft Lip and Palate for Prognosis of Alveolar Bone Grafting</title><author>Liu, Luwei ; Zhang, Qian ; Lin, Jiuxiang ; Ma, Lian ; Zhou, Zhibo ; He, Xuesong ; Jia, Yilin ; Chen, Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-6c523ff93ebb330df67e63866bcc45f376a0132b03dc278282e2c0c2284326cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Abundance</topic><topic>Adolescent</topic><topic>Alveolar bone</topic><topic>Alveolar Bone Grafting</topic><topic>Analysis</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Biodiversity</topic><topic>Biofilms</topic><topic>Biology and Life Sciences</topic><topic>Bone composition</topic><topic>Bone grafts</topic><topic>Care and treatment</topic><topic>Child</topic><topic>Children</topic><topic>Cleft lip</topic><topic>Cleft Lip - microbiology</topic><topic>Cleft Lip - surgery</topic><topic>Cleft lip/palate</topic><topic>Cleft Palate - microbiology</topic><topic>Cleft Palate - surgery</topic><topic>Ecology and Environmental Sciences</topic><topic>Female</topic><topic>Fistula</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Girls</topic><topic>Grafting</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Investigations</topic><topic>Laboratories</topic><topic>Male</topic><topic>Maxillofacial surgery</topic><topic>Medical prognosis</topic><topic>Medicine and Health Sciences</topic><topic>Metagenome</topic><topic>Metagenomics - methods</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Microorganisms</topic><topic>Mouth - microbiology</topic><topic>Oral cavity</topic><topic>Orthodontics</topic><topic>Pathogenesis</topic><topic>Patient outcomes</topic><topic>Physical growth</topic><topic>Physiological aspects</topic><topic>Porphyromonas gingivalis</topic><topic>Preoperative Period</topic><topic>Principal components analysis</topic><topic>Prognosis</topic><topic>Relative abundance</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Saliva</topic><topic>Saliva - microbiology</topic><topic>Skin & tissue grafts</topic><topic>Studies</topic><topic>Substitute bone</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Luwei</creatorcontrib><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Lin, Jiuxiang</creatorcontrib><creatorcontrib>Ma, Lian</creatorcontrib><creatorcontrib>Zhou, Zhibo</creatorcontrib><creatorcontrib>He, Xuesong</creatorcontrib><creatorcontrib>Jia, Yilin</creatorcontrib><creatorcontrib>Chen, Feng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Luwei</au><au>Zhang, Qian</au><au>Lin, Jiuxiang</au><au>Ma, Lian</au><au>Zhou, Zhibo</au><au>He, Xuesong</au><au>Jia, Yilin</au><au>Chen, Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigating Oral Microbiome Profiles in Children with Cleft Lip and Palate for Prognosis of Alveolar Bone Grafting</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-05-18</date><risdate>2016</risdate><volume>11</volume><issue>5</issue><spage>e0155683</spage><epage>e0155683</epage><pages>e0155683-e0155683</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In this study, we sought to investigate the oral microbiota structure of children with cleft lip and palate (CLP) and explore the pre-operative oral bacterial composition related to the prognosis of alveolar bone grafting. In total, 28 patients (19 boys, 9 girls) with CLP who were scheduled to undergo alveolar bone grafting for the first time were recruited. According to the clinical examination of operative sites at the third month after the operation, the individuals were divided into a non-inflammation group (n = 15) and an inflammation group (n = 13). In all, 56 unstimulated saliva samples were collected before and after the operation. The v3-v4 hypervariable regions of the 16S rRNA gene were sequenced using an Illumina MiSeq sequencing platform. Based on the beta diversity of the operational taxonomic units (OTUs) in the inflammation and non-inflammation samples, the microbial variation in the oral cavity differed significantly between the two groups before and after the operation (P < 0.05). Analysis of the relative abundances of pre-operative OTUs revealed 26 OTUs with a relative abundance higher than 0.01%, reflecting a significant difference of the relative abundance between groups (P < 0.05). According to a principal component analysis of the pre-operative samples, the inflammation-related OTUs included Tannerella sp., Porphyromonas sp., Gemella sp., Moraxella sp., Prevotella nigrescens, and Prevotella intermedia, most of which were enriched in the inflammation group and showed a significant positive correlation. A cross-validated random forest model based on the 26 different OTUs before the operation was able to fit the post-operative status of grafted sites and yielded a good classification result. The sensitivity and specificity of this classified model were 76.9% and 86.7%, respectively. These findings show that the oral microbiota profile before alveolar bone grafting may be related to the risk of post-operative inflammation at grafted sites.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27191390</pmid><doi>10.1371/journal.pone.0155683</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-05, Vol.11 (5), p.e0155683-e0155683 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1789766306 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Abundance Adolescent Alveolar bone Alveolar Bone Grafting Analysis Antibiotics Bacteria Biodiversity Biofilms Biology and Life Sciences Bone composition Bone grafts Care and treatment Child Children Cleft lip Cleft Lip - microbiology Cleft Lip - surgery Cleft lip/palate Cleft Palate - microbiology Cleft Palate - surgery Ecology and Environmental Sciences Female Fistula Gene expression Gene sequencing Girls Grafting Hospitals Humans Infections Inflammation Investigations Laboratories Male Maxillofacial surgery Medical prognosis Medicine and Health Sciences Metagenome Metagenomics - methods Microbiomes Microbiota Microbiota (Symbiotic organisms) Microorganisms Mouth - microbiology Oral cavity Orthodontics Pathogenesis Patient outcomes Physical growth Physiological aspects Porphyromonas gingivalis Preoperative Period Principal components analysis Prognosis Relative abundance RNA, Ribosomal, 16S - genetics rRNA 16S Saliva Saliva - microbiology Skin & tissue grafts Studies Substitute bone Surgery |
| title | Investigating Oral Microbiome Profiles in Children with Cleft Lip and Palate for Prognosis of Alveolar Bone Grafting |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T03%3A38%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Investigating%20Oral%20Microbiome%20Profiles%20in%20Children%20with%20Cleft%20Lip%20and%20Palate%20for%20Prognosis%20of%20Alveolar%20Bone%20Grafting&rft.jtitle=PloS%20one&rft.au=Liu,%20Luwei&rft.date=2016-05-18&rft.volume=11&rft.issue=5&rft.spage=e0155683&rft.epage=e0155683&rft.pages=e0155683-e0155683&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0155683&rft_dat=%3Cgale_plos_%3EA453360307%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1789766306&rft_id=info:pmid/27191390&rft_galeid=A453360307&rft_doaj_id=oai_doaj_org_article_55802a0eefd64c228efcc6a2f71f50fa&rfr_iscdi=true |