Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis
Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2016-04, Vol.10 (4), p.e0004634-e0004634 |
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| creator | Huang, Fuqiang Dang, Zhisheng Suzuki, Yutaka Horiuchi, Terumi Yagi, Kinpei Kouguchi, Hirokazu Irie, Takao Kim, Kyeongsoon Oku, Yuzaburo |
| description | Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development. |
| doi_str_mv | 10.1371/journal.pntd.0004634 |
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Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0004634</identifier><identifier>PMID: 27092774</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antigens ; Antigens, Helminth - biosynthesis ; Biology and Life Sciences ; Echinococcus multilocularis ; Echinococcus multilocularis - genetics ; Echinococcus multilocularis - growth & development ; Eggs ; Gene expression ; Genetic aspects ; Genomes ; Heat shock proteins ; High-Throughput Nucleotide Sequencing ; Infections ; Larvae ; Liver ; Medicine and Health Sciences ; Parasites ; Public health ; Tapeworms ; Transcriptome ; Tropical diseases ; Worms ; Zoonoses</subject><ispartof>PLoS neglected tropical diseases, 2016-04, Vol.10 (4), p.e0004634-e0004634</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: . PLoS Negl Trop Dis 10(4): e0004634. doi:10.1371/journal.pntd.0004634</rights><rights>2016 Huang et al 2016 Huang et al</rights><rights>2016 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: . 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The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.</description><subject>Animals</subject><subject>Antigens</subject><subject>Antigens, Helminth - biosynthesis</subject><subject>Biology and Life Sciences</subject><subject>Echinococcus multilocularis</subject><subject>Echinococcus multilocularis - genetics</subject><subject>Echinococcus multilocularis - growth & development</subject><subject>Eggs</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Heat shock proteins</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Infections</subject><subject>Larvae</subject><subject>Liver</subject><subject>Medicine and Health Sciences</subject><subject>Parasites</subject><subject>Public health</subject><subject>Tapeworms</subject><subject>Transcriptome</subject><subject>Tropical diseases</subject><subject>Worms</subject><subject>Zoonoses</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqFklGL1DAUhYso7rr6D0QLgvgyY9IkTfMiDMu4LqysoD6HbHozkyVNatKK8-9Nd7rLjCzYPDTpPffk9uMUxWuMlphw_PE2jNErt-z90C4RQrQm9ElxigVhi4oT9vRgf1K8SOkWISZYg58XJxVHouKcnhb9Knvskk1l8OUFeCjXf_oIKdl8_haDsQ5K68trr0Pqt5BLpfJtuVbR7crvg9pA-RUGpSENoc1FE0NXrvXW-qCD1mMqu9EN1gU9OhVtelk8M8oleDW_z4qfn9c_zr8srq4vLs9XVwvNKzIsMCUtFZzWleZEmKrBqmU4b0yDDGuYrphi_EZgrCkFpFVlal5DS0BQjStEzoq3e9_ehSRnWEli3ghGa45wVlzuFW1Qt7KPtlNxJ4Oy8u5DiBup4mC1A6mwblpaAVNI0PyoShsKZqJuoEE8e32abxtvOmg1-CEqd2R6XPF2Kzfht6QNqWsxDfNhNojh15hhys4mDc4pD2Gc5hZI1A3JXP4vbQihGJMJwrt_pI-DmFUblf_VehPyiHoylSvKBSO1uAO6fESVVwud1cHDFJXjhvcHDVtQbtim4MYhRysdC-leqGNIKYJ54IaRnLJ-P7Wcsi7nrOe2N4fMH5ruw03-AmgL-uk</recordid><startdate>20160419</startdate><enddate>20160419</enddate><creator>Huang, Fuqiang</creator><creator>Dang, Zhisheng</creator><creator>Suzuki, Yutaka</creator><creator>Horiuchi, Terumi</creator><creator>Yagi, Kinpei</creator><creator>Kouguchi, Hirokazu</creator><creator>Irie, Takao</creator><creator>Kim, Kyeongsoon</creator><creator>Oku, Yuzaburo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7U2</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1447-9916</orcidid></search><sort><creationdate>20160419</creationdate><title>Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis</title><author>Huang, Fuqiang ; Dang, Zhisheng ; Suzuki, Yutaka ; Horiuchi, Terumi ; Yagi, Kinpei ; Kouguchi, Hirokazu ; Irie, Takao ; Kim, Kyeongsoon ; Oku, Yuzaburo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c723t-143d497462c739f281ad519f2f80f585c25a57b911c44e0ca2f676ed3e94c1203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Antigens, Helminth - 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Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27092774</pmid><doi>10.1371/journal.pntd.0004634</doi><orcidid>https://orcid.org/0000-0003-1447-9916</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigens Antigens, Helminth - biosynthesis Biology and Life Sciences Echinococcus multilocularis Echinococcus multilocularis - genetics Echinococcus multilocularis - growth & development Eggs Gene expression Genetic aspects Genomes Heat shock proteins High-Throughput Nucleotide Sequencing Infections Larvae Liver Medicine and Health Sciences Parasites Public health Tapeworms Transcriptome Tropical diseases Worms Zoonoses |
| title | Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis |
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