Complete Nucleotide Sequence of IncP-1β Plasmid pDTC28 Reveals a Non-Functional Variant of the blaGES-Type Gene
Plasmid pDTC28 was isolated from the sediments of Haihe River using E. coli CV601 (gfp-tagged) as recipient and indigenous bacteria from the sediment as donors. This plasmid confers reduced susceptibility to tetracycline and sulfamethoxazole. The complete sequence of plasmid pDTC28 was 61,503 bp in...
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| description | Plasmid pDTC28 was isolated from the sediments of Haihe River using E. coli CV601 (gfp-tagged) as recipient and indigenous bacteria from the sediment as donors. This plasmid confers reduced susceptibility to tetracycline and sulfamethoxazole. The complete sequence of plasmid pDTC28 was 61,503 bp in length with an average G+C content of 64.09%. Plasmid pDTC28 belongs to the IncP-1β group by phylogenetic analysis. The backbones of plasmid pDTC28 and other IncP-1β plasmids are very classical and conserved, whereas the accessory regions of these plasmids are diverse. A blaGES-5-like gene was found on the accessory region, and this blaGES-5-like gene contained 18 silent mutations and 7 missense mutations compared with the blaGES-5 gene. The mutations resulted in 7 amino acid substitutions in GES-5 carbapenemase, causing the loss of function of the blaGES-5-like gene on plasmid pDTC28 against carbapenems and even β-lactams. The enzyme produced by the blaGES-5-like gene cassette may be a new variant of GES-type enzymes. Thus, the plasmid sequenced in this study will expand our understanding of GES-type β-lactamases and provide insights into the genetic platforms used for the dissemination of GES-type genes. |
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This plasmid confers reduced susceptibility to tetracycline and sulfamethoxazole. The complete sequence of plasmid pDTC28 was 61,503 bp in length with an average G+C content of 64.09%. Plasmid pDTC28 belongs to the IncP-1β group by phylogenetic analysis. The backbones of plasmid pDTC28 and other IncP-1β plasmids are very classical and conserved, whereas the accessory regions of these plasmids are diverse. A blaGES-5-like gene was found on the accessory region, and this blaGES-5-like gene contained 18 silent mutations and 7 missense mutations compared with the blaGES-5 gene. The mutations resulted in 7 amino acid substitutions in GES-5 carbapenemase, causing the loss of function of the blaGES-5-like gene on plasmid pDTC28 against carbapenems and even β-lactams. The enzyme produced by the blaGES-5-like gene cassette may be a new variant of GES-type enzymes. Thus, the plasmid sequenced in this study will expand our understanding of GES-type β-lactamases and provide insights into the genetic platforms used for the dissemination of GES-type genes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0154975</identifier><identifier>PMID: 27152950</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino acids ; Antibiotics ; Antimicrobial agents ; Bacteria ; Bacteriology ; Biology and life sciences ; Carbapenemase ; Carbapenems ; Chemotherapy ; Drug resistance ; E coli ; Engineering ; Engineering and Technology ; Environmental science ; Escherichia coli ; Escherichia coli - genetics ; Fluvial sediments ; Genes ; Geologic Sediments - microbiology ; Gram-negative bacteria ; Laboratories ; Medicine and Health Sciences ; Missense mutation ; Mutation ; Nucleotide sequence ; Phylogeny ; Plasmids ; Research and Analysis Methods ; Rivers ; Sediments ; Sulfamethoxazole</subject><ispartof>PloS one, 2016-05, Vol.11 (5), p.e0154975-e0154975</ispartof><rights>2016 Dang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Dang et al 2016 Dang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4415-b26f1629976ec04422dfdcf739c589daef882776701baa15165526f15dda23803</citedby><cites>FETCH-LOGICAL-c4415-b26f1629976ec04422dfdcf739c589daef882776701baa15165526f15dda23803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859535/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859535/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27152950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hayes, Finbarr</contributor><creatorcontrib>Dang, Bingjun</creatorcontrib><creatorcontrib>Mao, Daqing</creatorcontrib><creatorcontrib>Luo, Yi</creatorcontrib><title>Complete Nucleotide Sequence of IncP-1β Plasmid pDTC28 Reveals a Non-Functional Variant of the blaGES-Type Gene</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Plasmid pDTC28 was isolated from the sediments of Haihe River using E. coli CV601 (gfp-tagged) as recipient and indigenous bacteria from the sediment as donors. This plasmid confers reduced susceptibility to tetracycline and sulfamethoxazole. The complete sequence of plasmid pDTC28 was 61,503 bp in length with an average G+C content of 64.09%. Plasmid pDTC28 belongs to the IncP-1β group by phylogenetic analysis. The backbones of plasmid pDTC28 and other IncP-1β plasmids are very classical and conserved, whereas the accessory regions of these plasmids are diverse. A blaGES-5-like gene was found on the accessory region, and this blaGES-5-like gene contained 18 silent mutations and 7 missense mutations compared with the blaGES-5 gene. The mutations resulted in 7 amino acid substitutions in GES-5 carbapenemase, causing the loss of function of the blaGES-5-like gene on plasmid pDTC28 against carbapenems and even β-lactams. The enzyme produced by the blaGES-5-like gene cassette may be a new variant of GES-type enzymes. Thus, the plasmid sequenced in this study will expand our understanding of GES-type β-lactamases and provide insights into the genetic platforms used for the dissemination of GES-type genes.</description><subject>Amino acids</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biology and life sciences</subject><subject>Carbapenemase</subject><subject>Carbapenems</subject><subject>Chemotherapy</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Engineering</subject><subject>Engineering and Technology</subject><subject>Environmental science</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fluvial sediments</subject><subject>Genes</subject><subject>Geologic Sediments - microbiology</subject><subject>Gram-negative bacteria</subject><subject>Laboratories</subject><subject>Medicine and Health Sciences</subject><subject>Missense mutation</subject><subject>Mutation</subject><subject>Nucleotide sequence</subject><subject>Phylogeny</subject><subject>Plasmids</subject><subject>Research and Analysis Methods</subject><subject>Rivers</subject><subject>Sediments</subject><subject>Sulfamethoxazole</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUs1u1DAYjBCIlsIbILDEhUsW_8b2BQkt7bJSVSq6cLUc-0ubVWKHOKnU1-qD9JnIsmnVIk627Jn55htNlr0leEGYJJ-2ceyDbRZdDLDARHAtxbPskGhG84Ji9vzR_SB7ldIWY8FUUbzMDqgkgmqBD7NuGduugQHQ2egaiEPtAV3A7xGCAxQrtA7uPCd3t-i8samtPeq-bpZUoR9wDbZJyKKzGPKTMbihjpMf9Mv2tQ3DjjtcASobuzq-yDc3HaAVBHidvagmHryZz6Ps58nxZvktP_2-Wi-_nOaOcyLykhYVKajWsgCHOafUV95VkmknlPYWKqWolIXEpLSWCFIIsaMI7y1lCrOj7P1et2tiMnNYyRCpJBWFIGpCrPcIH-3WdH3d2v7GRFubvw-xvzS2H-opFVNJjkEpXlrmeVVSKyxTwIBp7LyjetL6PE8byxa8gzD0tnki-vQn1FfmMl4broQWTEwCH2eBPk7hp8G0dXLQNDZAHPe-NSsIIxP0wz_Q_2_H9yjXx5R6qB7MEGx2BbpnmV2BzFygifbu8SIPpPvGsD9zL8MM</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Dang, Bingjun</creator><creator>Mao, Daqing</creator><creator>Luo, Yi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160501</creationdate><title>Complete Nucleotide Sequence of IncP-1β Plasmid pDTC28 Reveals a Non-Functional Variant of the blaGES-Type Gene</title><author>Dang, Bingjun ; Mao, Daqing ; Luo, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4415-b26f1629976ec04422dfdcf739c589daef882776701baa15165526f15dda23803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino acids</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biology and life sciences</topic><topic>Carbapenemase</topic><topic>Carbapenems</topic><topic>Chemotherapy</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Engineering</topic><topic>Engineering and Technology</topic><topic>Environmental science</topic><topic>Escherichia coli</topic><topic>Escherichia coli - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dang, Bingjun</au><au>Mao, Daqing</au><au>Luo, Yi</au><au>Hayes, Finbarr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete Nucleotide Sequence of IncP-1β Plasmid pDTC28 Reveals a Non-Functional Variant of the blaGES-Type Gene</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>11</volume><issue>5</issue><spage>e0154975</spage><epage>e0154975</epage><pages>e0154975-e0154975</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Plasmid pDTC28 was isolated from the sediments of Haihe River using E. coli CV601 (gfp-tagged) as recipient and indigenous bacteria from the sediment as donors. This plasmid confers reduced susceptibility to tetracycline and sulfamethoxazole. The complete sequence of plasmid pDTC28 was 61,503 bp in length with an average G+C content of 64.09%. Plasmid pDTC28 belongs to the IncP-1β group by phylogenetic analysis. The backbones of plasmid pDTC28 and other IncP-1β plasmids are very classical and conserved, whereas the accessory regions of these plasmids are diverse. A blaGES-5-like gene was found on the accessory region, and this blaGES-5-like gene contained 18 silent mutations and 7 missense mutations compared with the blaGES-5 gene. The mutations resulted in 7 amino acid substitutions in GES-5 carbapenemase, causing the loss of function of the blaGES-5-like gene on plasmid pDTC28 against carbapenems and even β-lactams. The enzyme produced by the blaGES-5-like gene cassette may be a new variant of GES-type enzymes. Thus, the plasmid sequenced in this study will expand our understanding of GES-type β-lactamases and provide insights into the genetic platforms used for the dissemination of GES-type genes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27152950</pmid><doi>10.1371/journal.pone.0154975</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acids Antibiotics Antimicrobial agents Bacteria Bacteriology Biology and life sciences Carbapenemase Carbapenems Chemotherapy Drug resistance E coli Engineering Engineering and Technology Environmental science Escherichia coli Escherichia coli - genetics Fluvial sediments Genes Geologic Sediments - microbiology Gram-negative bacteria Laboratories Medicine and Health Sciences Missense mutation Mutation Nucleotide sequence Phylogeny Plasmids Research and Analysis Methods Rivers Sediments Sulfamethoxazole |
| title | Complete Nucleotide Sequence of IncP-1β Plasmid pDTC28 Reveals a Non-Functional Variant of the blaGES-Type Gene |
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