Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell Lines

The snoMEN (snoRNA Modulator of gene ExpressioN) vector technology was developed from a human box C/D snoRNA, HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets, allowing knock-down of targeted genes. Here we have screened additional human...

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Veröffentlicht in:	PloS one 2016-04, Vol.11 (4), p.e0154759-e0154759
Hauptverfasser:	Ono, Motoharu, Yamada, Kayo, Bensaddek, Dalila, Afzal, Vackar, Biddlestone, John, Ortmann, Brian, Mudie, Sharon, Boivin, Vincent, Scott, Michelle S, Rocha, Sonia, Lamond, Angus I
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biochemistry Biology and life sciences Biotechnology Cell cycle Cell Line Cell lines Cyclin-dependent kinases Efficiency Expression vectors Gene Expression Gene Knockdown Techniques - methods Gene therapy Genetic Vectors Green Fluorescent Proteins - genetics Health sciences HeLa Cells Humans Hypoxia-Inducible Factor 1, alpha Subunit - genetics Kinases Life sciences Mass spectrometry Mass spectroscopy Multiplexing Nucleic Acid Conformation Nucleoli Nucleotide sequence Protein Binding Proteins Proteomics Recombinant Fusion Proteins - genetics Research and analysis methods Ribonucleic acid Ribonucleoproteins, Small Nucleolar - metabolism RNA RNA polymerase RNA, Small Nucleolar - chemistry RNA, Small Nucleolar - genetics RNA, Small Nucleolar - metabolism snoRNA Technology Transcription
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