Association between Polycystic Ovary Syndrome and Gut Microbiota
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromat...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-04, Vol.11 (4), p.e0153196-e0153196 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0153196 |
|---|---|
| container_issue | 4 |
| container_start_page | e0153196 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Guo, Yanjie Qi, Yane Yang, Xuefei Zhao, Lihui Wen, Shu Liu, Yinhui Tang, Li |
| description | Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats. |
| doi_str_mv | 10.1371/journal.pone.0153196 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1782205374</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A453447939</galeid><doaj_id>oai_doaj_org_article_fe6f6b01507d4e878851455d63d14a63</doaj_id><sourcerecordid>A453447939</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-a8e177686a963bd360a522ec6bf749bd070ee77a46ae4a51c9cad891d5bc7d543</originalsourceid><addsrcrecordid>eNqNklFv0zAUhSMEYmPwDxBEQkLw0GLHju28IKoJRqWhIga8Wo5907pK4hI7g_57nDWbGrQH5Adb9nePfa5PkjzHaI4Jx--2ru9aVc93roU5wjnBBXuQnOKCZDOWIfLwaH2SPPF-i1BOBGOPk5OMo4Iwmp0mHxbeO21VsK5NSwi_Adr0q6v3eu-D1enqWnX79Grfms41kKrWpBd9SL9Y3bnSuqCeJo8qVXt4Ns5nyY9PH7-ff55dri6W54vLmea5CDMlAHPOBFMFI6UhDKk8y0CzsuK0KA3iCIBzRZkCqnKsC62MKLDJS81NTslZ8vKgu6udl6N5LzEXWRZ98YFYHgjj1FbuOtvEp0unrLzZcN1aqi56qkFWwCpWxqYhbigILkSOaZ4bRgymipGo9X68rS8bMBra0Kl6Ijo9ae1Grt21pIIwTlkUeDMKdO5XDz7IxnoNda1acP3NuwkhgpMBffUPer-7kVqraMC2lYv36kFULmhOKOUFKSI1v4eKw0BjdUxKZeP-pODtpCAyAf6Eteq9l8urb__Prn5O2ddH7AZUHTbe1f2QMz8F6QGMgfK-g-quyRjJIei33ZBD0OUY9Fj24viD7opuk03-Aul290Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1782205374</pqid></control><display><type>article</type><title>Association between Polycystic Ovary Syndrome and Gut Microbiota</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Guo, Yanjie ; Qi, Yane ; Yang, Xuefei ; Zhao, Lihui ; Wen, Shu ; Liu, Yinhui ; Tang, Li</creator><creatorcontrib>Guo, Yanjie ; Qi, Yane ; Yang, Xuefei ; Zhao, Lihui ; Wen, Shu ; Liu, Yinhui ; Tang, Li</creatorcontrib><description>Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0153196</identifier><identifier>PMID: 27093642</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal tissues ; Animals ; Aromatase ; Aromatase Inhibitors - pharmacology ; Biology and Life Sciences ; Biosynthesis ; Care and treatment ; Cysts ; Development and progression ; Diabetes ; Disease ; Dosage and administration ; Dysbacteriosis ; Endocrine disorders ; Endocrinology ; Estrous Cycle - drug effects ; Estrous Cycle - physiology ; Estrus ; Etiology ; Fecal microflora ; Feces ; Female ; Fibroblasts ; Gastrointestinal Microbiome - drug effects ; Gastrointestinal Microbiome - physiology ; Immune system ; Insulin resistance ; Intestinal microflora ; Lactobacillus ; Letrozole ; Medical research ; Medicine and Health Sciences ; Metabolism ; Metabolites ; Microbiota ; Microbiota (Symbiotic organisms) ; Microbiota - drug effects ; Nitriles - pharmacology ; Obesity ; Ovary - pathology ; Pathogenesis ; Pathogens ; Physiological aspects ; Polycystic ovary syndrome ; Polycystic Ovary Syndrome - drug therapy ; Polycystic Ovary Syndrome - pathology ; Rats ; Rats, Sprague-Dawley ; Research and Analysis Methods ; Researchers ; Rodents ; Science ; Studies ; Testosterone ; Transplantation ; Triazoles - pharmacology ; Womens health</subject><ispartof>PloS one, 2016-04, Vol.11 (4), p.e0153196-e0153196</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Guo et al 2016 Guo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-a8e177686a963bd360a522ec6bf749bd070ee77a46ae4a51c9cad891d5bc7d543</citedby><cites>FETCH-LOGICAL-c758t-a8e177686a963bd360a522ec6bf749bd070ee77a46ae4a51c9cad891d5bc7d543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836746/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836746/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27093642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Yanjie</creatorcontrib><creatorcontrib>Qi, Yane</creatorcontrib><creatorcontrib>Yang, Xuefei</creatorcontrib><creatorcontrib>Zhao, Lihui</creatorcontrib><creatorcontrib>Wen, Shu</creatorcontrib><creatorcontrib>Liu, Yinhui</creatorcontrib><creatorcontrib>Tang, Li</creatorcontrib><title>Association between Polycystic Ovary Syndrome and Gut Microbiota</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.</description><subject>Analysis</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Aromatase</subject><subject>Aromatase Inhibitors - pharmacology</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Care and treatment</subject><subject>Cysts</subject><subject>Development and progression</subject><subject>Diabetes</subject><subject>Disease</subject><subject>Dosage and administration</subject><subject>Dysbacteriosis</subject><subject>Endocrine disorders</subject><subject>Endocrinology</subject><subject>Estrous Cycle - drug effects</subject><subject>Estrous Cycle - physiology</subject><subject>Estrus</subject><subject>Etiology</subject><subject>Fecal microflora</subject><subject>Feces</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Gastrointestinal Microbiome - physiology</subject><subject>Immune system</subject><subject>Insulin resistance</subject><subject>Intestinal microflora</subject><subject>Lactobacillus</subject><subject>Letrozole</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microbiota - drug effects</subject><subject>Nitriles - pharmacology</subject><subject>Obesity</subject><subject>Ovary - pathology</subject><subject>Pathogenesis</subject><subject>Pathogens</subject><subject>Physiological aspects</subject><subject>Polycystic ovary syndrome</subject><subject>Polycystic Ovary Syndrome - drug therapy</subject><subject>Polycystic Ovary Syndrome - pathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Research and Analysis Methods</subject><subject>Researchers</subject><subject>Rodents</subject><subject>Science</subject><subject>Studies</subject><subject>Testosterone</subject><subject>Transplantation</subject><subject>Triazoles - pharmacology</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNklFv0zAUhSMEYmPwDxBEQkLw0GLHju28IKoJRqWhIga8Wo5907pK4hI7g_57nDWbGrQH5Adb9nePfa5PkjzHaI4Jx--2ru9aVc93roU5wjnBBXuQnOKCZDOWIfLwaH2SPPF-i1BOBGOPk5OMo4Iwmp0mHxbeO21VsK5NSwi_Adr0q6v3eu-D1enqWnX79Grfms41kKrWpBd9SL9Y3bnSuqCeJo8qVXt4Ns5nyY9PH7-ff55dri6W54vLmea5CDMlAHPOBFMFI6UhDKk8y0CzsuK0KA3iCIBzRZkCqnKsC62MKLDJS81NTslZ8vKgu6udl6N5LzEXWRZ98YFYHgjj1FbuOtvEp0unrLzZcN1aqi56qkFWwCpWxqYhbigILkSOaZ4bRgymipGo9X68rS8bMBra0Kl6Ijo9ae1Grt21pIIwTlkUeDMKdO5XDz7IxnoNda1acP3NuwkhgpMBffUPer-7kVqraMC2lYv36kFULmhOKOUFKSI1v4eKw0BjdUxKZeP-pODtpCAyAf6Eteq9l8urb__Prn5O2ddH7AZUHTbe1f2QMz8F6QGMgfK-g-quyRjJIei33ZBD0OUY9Fj24viD7opuk03-Aul290Q</recordid><startdate>20160419</startdate><enddate>20160419</enddate><creator>Guo, Yanjie</creator><creator>Qi, Yane</creator><creator>Yang, Xuefei</creator><creator>Zhao, Lihui</creator><creator>Wen, Shu</creator><creator>Liu, Yinhui</creator><creator>Tang, Li</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160419</creationdate><title>Association between Polycystic Ovary Syndrome and Gut Microbiota</title><author>Guo, Yanjie ; Qi, Yane ; Yang, Xuefei ; Zhao, Lihui ; Wen, Shu ; Liu, Yinhui ; Tang, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-a8e177686a963bd360a522ec6bf749bd070ee77a46ae4a51c9cad891d5bc7d543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Aromatase</topic><topic>Aromatase Inhibitors - pharmacology</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Care and treatment</topic><topic>Cysts</topic><topic>Development and progression</topic><topic>Diabetes</topic><topic>Disease</topic><topic>Dosage and administration</topic><topic>Dysbacteriosis</topic><topic>Endocrine disorders</topic><topic>Endocrinology</topic><topic>Estrous Cycle - drug effects</topic><topic>Estrous Cycle - physiology</topic><topic>Estrus</topic><topic>Etiology</topic><topic>Fecal microflora</topic><topic>Feces</topic><topic>Female</topic><topic>Fibroblasts</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>Gastrointestinal Microbiome - physiology</topic><topic>Immune system</topic><topic>Insulin resistance</topic><topic>Intestinal microflora</topic><topic>Lactobacillus</topic><topic>Letrozole</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Microbiota</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Microbiota - drug effects</topic><topic>Nitriles - pharmacology</topic><topic>Obesity</topic><topic>Ovary - pathology</topic><topic>Pathogenesis</topic><topic>Pathogens</topic><topic>Physiological aspects</topic><topic>Polycystic ovary syndrome</topic><topic>Polycystic Ovary Syndrome - drug therapy</topic><topic>Polycystic Ovary Syndrome - pathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Research and Analysis Methods</topic><topic>Researchers</topic><topic>Rodents</topic><topic>Science</topic><topic>Studies</topic><topic>Testosterone</topic><topic>Transplantation</topic><topic>Triazoles - pharmacology</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Yanjie</creatorcontrib><creatorcontrib>Qi, Yane</creatorcontrib><creatorcontrib>Yang, Xuefei</creatorcontrib><creatorcontrib>Zhao, Lihui</creatorcontrib><creatorcontrib>Wen, Shu</creatorcontrib><creatorcontrib>Liu, Yinhui</creatorcontrib><creatorcontrib>Tang, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Yanjie</au><au>Qi, Yane</au><au>Yang, Xuefei</au><au>Zhao, Lihui</au><au>Wen, Shu</au><au>Liu, Yinhui</au><au>Tang, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association between Polycystic Ovary Syndrome and Gut Microbiota</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-04-19</date><risdate>2016</risdate><volume>11</volume><issue>4</issue><spage>e0153196</spage><epage>e0153196</epage><pages>e0153196-e0153196</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27093642</pmid><doi>10.1371/journal.pone.0153196</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-04, Vol.11 (4), p.e0153196-e0153196 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1782205374 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analysis Animal tissues Animals Aromatase Aromatase Inhibitors - pharmacology Biology and Life Sciences Biosynthesis Care and treatment Cysts Development and progression Diabetes Disease Dosage and administration Dysbacteriosis Endocrine disorders Endocrinology Estrous Cycle - drug effects Estrous Cycle - physiology Estrus Etiology Fecal microflora Feces Female Fibroblasts Gastrointestinal Microbiome - drug effects Gastrointestinal Microbiome - physiology Immune system Insulin resistance Intestinal microflora Lactobacillus Letrozole Medical research Medicine and Health Sciences Metabolism Metabolites Microbiota Microbiota (Symbiotic organisms) Microbiota - drug effects Nitriles - pharmacology Obesity Ovary - pathology Pathogenesis Pathogens Physiological aspects Polycystic ovary syndrome Polycystic Ovary Syndrome - drug therapy Polycystic Ovary Syndrome - pathology Rats Rats, Sprague-Dawley Research and Analysis Methods Researchers Rodents Science Studies Testosterone Transplantation Triazoles - pharmacology Womens health |
| title | Association between Polycystic Ovary Syndrome and Gut Microbiota |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T06%3A12%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Association%20between%20Polycystic%20Ovary%20Syndrome%20and%20Gut%20Microbiota&rft.jtitle=PloS%20one&rft.au=Guo,%20Yanjie&rft.date=2016-04-19&rft.volume=11&rft.issue=4&rft.spage=e0153196&rft.epage=e0153196&rft.pages=e0153196-e0153196&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0153196&rft_dat=%3Cgale_plos_%3EA453447939%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1782205374&rft_id=info:pmid/27093642&rft_galeid=A453447939&rft_doaj_id=oai_doaj_org_article_fe6f6b01507d4e878851455d63d14a63&rfr_iscdi=true |