Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus
Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and...
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| Veröffentlicht in: | PloS one 2016-04, Vol.11 (4), p.e0153019 |
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| creator | Maifeld, Sarah V Ro, Bodrey Mok, Hoyin Chu, Marla Yu, Li Yamagata, Ryan Leonardson, Tansy Chio, Vera Parhy, Bandita Park, Samuel Carlson, Marcia Machhi, Shushil Ulbrandt, Nancy Falsey, Ann R Walsh, Edward E Wang, C Kathy Esser, Mark T Zuo, Fengrong |
| description | Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population. |
| doi_str_mv | 10.1371/journal.pone.0153019 |
| format | Article |
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We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0153019</identifier><identifier>PMID: 27070145</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aged ; Analysis ; Antibodies, Monoclonal, Humanized - immunology ; Antibodies, Neutralizing - blood ; Antibodies, Viral - blood ; Antigens ; Antigens, Viral - immunology ; Assaying ; Biology and Life Sciences ; Care and treatment ; Causes of ; Child, Preschool ; Complications and side effects ; Dentistry ; Diagnostic systems ; Electrochemiluminescence ; Emitter-coupled logic ; Enzyme-Linked Immunosorbent Assay ; Geriatrics ; Health aspects ; Health care ; Humans ; Immune response (humoral) ; Immunity, Humoral ; Immunoglobulins ; Immunology ; Infant ; Infants ; Infections ; Luminescent Measurements - methods ; Medical diagnosis ; Medicine ; Medicine and Health Sciences ; Mortality ; Neutralization Tests ; Nucleocapsid Proteins - immunology ; Older people ; People and Places ; Proteins ; Research and Analysis Methods ; Respiratory syncytial virus ; Respiratory Syncytial Virus Infections - diagnosis ; Respiratory Syncytial Virus Infections - immunology ; Respiratory Syncytial Virus, Human - immunology ; Respiratory tract infections ; Risk factors ; Sensitivity ; Serologic Tests - methods ; Serology ; Technology application ; Vaccination ; Vaccines ; Viral Envelope Proteins - immunology ; Viral Fusion Proteins - immunology ; Viruses</subject><ispartof>PloS one, 2016-04, Vol.11 (4), p.e0153019</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Maifeld et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Maifeld et al 2016 Maifeld et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-6e37d30bc4a9d2eafaa3f7cafe5335090b7952130bebfbf59de7266c368a19f53</citedby><cites>FETCH-LOGICAL-c725t-6e37d30bc4a9d2eafaa3f7cafe5335090b7952130bebfbf59de7266c368a19f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829208/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829208/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27070145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maifeld, Sarah V</creatorcontrib><creatorcontrib>Ro, Bodrey</creatorcontrib><creatorcontrib>Mok, Hoyin</creatorcontrib><creatorcontrib>Chu, Marla</creatorcontrib><creatorcontrib>Yu, Li</creatorcontrib><creatorcontrib>Yamagata, Ryan</creatorcontrib><creatorcontrib>Leonardson, Tansy</creatorcontrib><creatorcontrib>Chio, Vera</creatorcontrib><creatorcontrib>Parhy, Bandita</creatorcontrib><creatorcontrib>Park, Samuel</creatorcontrib><creatorcontrib>Carlson, Marcia</creatorcontrib><creatorcontrib>Machhi, Shushil</creatorcontrib><creatorcontrib>Ulbrandt, Nancy</creatorcontrib><creatorcontrib>Falsey, Ann R</creatorcontrib><creatorcontrib>Walsh, Edward E</creatorcontrib><creatorcontrib>Wang, C Kathy</creatorcontrib><creatorcontrib>Esser, Mark T</creatorcontrib><creatorcontrib>Zuo, Fengrong</creatorcontrib><title>Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. 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| language | eng |
| recordid | cdi_plos_journals_1780486782 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aged Analysis Antibodies, Monoclonal, Humanized - immunology Antibodies, Neutralizing - blood Antibodies, Viral - blood Antigens Antigens, Viral - immunology Assaying Biology and Life Sciences Care and treatment Causes of Child, Preschool Complications and side effects Dentistry Diagnostic systems Electrochemiluminescence Emitter-coupled logic Enzyme-Linked Immunosorbent Assay Geriatrics Health aspects Health care Humans Immune response (humoral) Immunity, Humoral Immunoglobulins Immunology Infant Infants Infections Luminescent Measurements - methods Medical diagnosis Medicine Medicine and Health Sciences Mortality Neutralization Tests Nucleocapsid Proteins - immunology Older people People and Places Proteins Research and Analysis Methods Respiratory syncytial virus Respiratory Syncytial Virus Infections - diagnosis Respiratory Syncytial Virus Infections - immunology Respiratory Syncytial Virus, Human - immunology Respiratory tract infections Risk factors Sensitivity Serologic Tests - methods Serology Technology application Vaccination Vaccines Viral Envelope Proteins - immunology Viral Fusion Proteins - immunology Viruses |
| title | Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T13%3A42%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20Electrochemiluminescent%20Serology%20Assays%20to%20Measure%20the%20Humoral%20Response%20to%20Antigens%20of%20Respiratory%20Syncytial%20Virus&rft.jtitle=PloS%20one&rft.au=Maifeld,%20Sarah%20V&rft.date=2016-04-12&rft.volume=11&rft.issue=4&rft.spage=e0153019&rft.pages=e0153019-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0153019&rft_dat=%3Cgale_plos_%3EA453448372%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1780486782&rft_id=info:pmid/27070145&rft_galeid=A453448372&rft_doaj_id=oai_doaj_org_article_e38e856e2cd84e9988ee2b82cce8bc51&rfr_iscdi=true |