The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis
Mycobacterium tuberculosis (M.tb) remains a successful pathogen, causing tuberculosis disease numbers to constantly increase. Although great progress has been made in delineating the disease, the host-pathogen interaction is incompletely described. B cells have shown to function as both effectors an...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-04, Vol.11 (4), p.e0152710-e0152710 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0152710 |
|---|---|
| container_issue | 4 |
| container_start_page | e0152710 |
| container_title | PloS one |
| container_volume | 11 |
| creator | du Plessis, Willem J Kleynhans, Léanie du Plessis, Nelita Stanley, Kim Malherbe, Stephanus T Maasdorp, Elizna Ronacher, Katharina Chegou, Novel N Walzl, Gerhard Loxton, Andre G |
| description | Mycobacterium tuberculosis (M.tb) remains a successful pathogen, causing tuberculosis disease numbers to constantly increase. Although great progress has been made in delineating the disease, the host-pathogen interaction is incompletely described. B cells have shown to function as both effectors and regulators of immunity via non-humoral methods in both innate and adaptive immune settings. Here we assessed specific B cell functional interaction following stimulation with a broad range of antigens within the LTBI milieu. Our results indicate that B cells readily produce pro- and anti-inflammatory cytokines (including IL-1β, IL-10, IL-17, IL-21 and TNF-α) in response to stimulation. TLR4 and TLR9 based stimulations achieved the greatest secreted cytokine-production response and BCG stimulation displayed a clear preference for inducing IL-1β production. We also show that the cytokines produced by B cells are implicated strongly in cell-mediated communication and that plasma (memory) B cells (CD19+CD27+CD138+) is the subset with the greatest contribution to cytokine production. Collectively our data provides insight into B cell responses, where they are implicated in and quantifies responses from specific B cell phenotypes. These findings warrant further functional B cell research with a focus on specific B cell phenotypes under conditions of active TB disease to further our knowledge about the contribution of various cell subsets which could have implications for future vaccine development or refined B cell orientated treatment in the health setting. |
| doi_str_mv | 10.1371/journal.pone.0152710 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1779033902</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A453452062</galeid><doaj_id>oai_doaj_org_article_bb3f865476bf4f689afebb5da0923231</doaj_id><sourcerecordid>A453452062</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-b041796c875a573736f3c53aa36d31412e94375b348c3c81c7f1684ac12fa88c3</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eJvO1wglYpBpUpDW-HWcly79ZTGne0g-Pc4NJsatIvJF7FOnvf18Tk-RfESginEHH649l1oVTPd-9ZMAaSIQ_CoOIUVRhOGAH58tD8pnsV4DQDFgrGnxQnigAIMxGmxXW1Ned61Ojmf3cpLE7NhNKW35edybpomlsmXsza5jWmdLq-S23WN6vGP5az8Hkzjdq5V4U_W7n1IvXKpkmlTuepqE3TX-Oji8-KJVU00L4bvWfHj_Mtq_m2yvPi6mM-WE80RTZMaEMgrpgWninLMMbNYU6wUZmsMCUSmIpjTGhOhsRZQcwuZIEpDZJXIsbPi9cF3n4-VQ5GihJxXAOMKoEwsDsTaq2u5D26Xk5deOfkv4MNGqpCcboysa2wFo4Sz2hLLRKWsqWu6VqBCGGGYvT4Np3X1zqx1vnVQzch0_Kd1W7nxvyQRCAmKs8G7wSD4m87EJHcu6lx21Rrf9XkLinDut3gAyishEGYko2_-Q-8vxEBtVL6ra63PKereVM4IxYQiwHpqeg-V19rsnM6Pz7ocHwnejwSZSeZ32qguRrm4unw4e_FzzL49YrdGNWkbfdP1TzGOQXIAdfAxBmPv-gGB7Gfnthqynx05zE6WvTru5Z3odljwX4omEf4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1779033902</pqid></control><display><type>article</type><title>The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>du Plessis, Willem J ; Kleynhans, Léanie ; du Plessis, Nelita ; Stanley, Kim ; Malherbe, Stephanus T ; Maasdorp, Elizna ; Ronacher, Katharina ; Chegou, Novel N ; Walzl, Gerhard ; Loxton, Andre G</creator><contributor>Wilkinson, Katalin Andrea</contributor><creatorcontrib>du Plessis, Willem J ; Kleynhans, Léanie ; du Plessis, Nelita ; Stanley, Kim ; Malherbe, Stephanus T ; Maasdorp, Elizna ; Ronacher, Katharina ; Chegou, Novel N ; Walzl, Gerhard ; Loxton, Andre G ; Wilkinson, Katalin Andrea</creatorcontrib><description>Mycobacterium tuberculosis (M.tb) remains a successful pathogen, causing tuberculosis disease numbers to constantly increase. Although great progress has been made in delineating the disease, the host-pathogen interaction is incompletely described. B cells have shown to function as both effectors and regulators of immunity via non-humoral methods in both innate and adaptive immune settings. Here we assessed specific B cell functional interaction following stimulation with a broad range of antigens within the LTBI milieu. Our results indicate that B cells readily produce pro- and anti-inflammatory cytokines (including IL-1β, IL-10, IL-17, IL-21 and TNF-α) in response to stimulation. TLR4 and TLR9 based stimulations achieved the greatest secreted cytokine-production response and BCG stimulation displayed a clear preference for inducing IL-1β production. We also show that the cytokines produced by B cells are implicated strongly in cell-mediated communication and that plasma (memory) B cells (CD19+CD27+CD138+) is the subset with the greatest contribution to cytokine production. Collectively our data provides insight into B cell responses, where they are implicated in and quantifies responses from specific B cell phenotypes. These findings warrant further functional B cell research with a focus on specific B cell phenotypes under conditions of active TB disease to further our knowledge about the contribution of various cell subsets which could have implications for future vaccine development or refined B cell orientated treatment in the health setting.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0152710</identifier><identifier>PMID: 27050308</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antigens ; Antigens, Bacterial - immunology ; B cells ; B-Lymphocytes - immunology ; Bacillus Calmette-Guerin vaccine ; BCG ; Biology and Life Sciences ; Care and treatment ; CD19 antigen ; CD27 antigen ; Cell interactions ; Cells, Cultured ; Cytokines ; Cytokines - biosynthesis ; Dosage and administration ; Humans ; Humoral immunity ; Immunity ; Immunologic Memory ; Immunological memory ; Inflammation ; Inflammation Mediators - metabolism ; Interleukin 10 ; Interleukin 17 ; Interleukin 21 ; Latent Tuberculosis - immunology ; Lipopolysaccharides - pharmacology ; Lymphocytes B ; Medicine and Health Sciences ; Memory cells ; Mycobacterium tuberculosis ; Pathogens ; Regulators ; Stimulation ; T cell receptors ; TLR4 protein ; TLR9 protein ; Toll-Like Receptor 9 - physiology ; Toll-like receptors ; Tuberculosis ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Vaccine development ; Vaccines</subject><ispartof>PloS one, 2016-04, Vol.11 (4), p.e0152710-e0152710</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 du Plessis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 du Plessis et al 2016 du Plessis et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-b041796c875a573736f3c53aa36d31412e94375b348c3c81c7f1684ac12fa88c3</citedby><cites>FETCH-LOGICAL-c725t-b041796c875a573736f3c53aa36d31412e94375b348c3c81c7f1684ac12fa88c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822853/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822853/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27050308$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wilkinson, Katalin Andrea</contributor><creatorcontrib>du Plessis, Willem J</creatorcontrib><creatorcontrib>Kleynhans, Léanie</creatorcontrib><creatorcontrib>du Plessis, Nelita</creatorcontrib><creatorcontrib>Stanley, Kim</creatorcontrib><creatorcontrib>Malherbe, Stephanus T</creatorcontrib><creatorcontrib>Maasdorp, Elizna</creatorcontrib><creatorcontrib>Ronacher, Katharina</creatorcontrib><creatorcontrib>Chegou, Novel N</creatorcontrib><creatorcontrib>Walzl, Gerhard</creatorcontrib><creatorcontrib>Loxton, Andre G</creatorcontrib><title>The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mycobacterium tuberculosis (M.tb) remains a successful pathogen, causing tuberculosis disease numbers to constantly increase. Although great progress has been made in delineating the disease, the host-pathogen interaction is incompletely described. B cells have shown to function as both effectors and regulators of immunity via non-humoral methods in both innate and adaptive immune settings. Here we assessed specific B cell functional interaction following stimulation with a broad range of antigens within the LTBI milieu. Our results indicate that B cells readily produce pro- and anti-inflammatory cytokines (including IL-1β, IL-10, IL-17, IL-21 and TNF-α) in response to stimulation. TLR4 and TLR9 based stimulations achieved the greatest secreted cytokine-production response and BCG stimulation displayed a clear preference for inducing IL-1β production. We also show that the cytokines produced by B cells are implicated strongly in cell-mediated communication and that plasma (memory) B cells (CD19+CD27+CD138+) is the subset with the greatest contribution to cytokine production. Collectively our data provides insight into B cell responses, where they are implicated in and quantifies responses from specific B cell phenotypes. These findings warrant further functional B cell research with a focus on specific B cell phenotypes under conditions of active TB disease to further our knowledge about the contribution of various cell subsets which could have implications for future vaccine development or refined B cell orientated treatment in the health setting.</description><subject>Antigens</subject><subject>Antigens, Bacterial - immunology</subject><subject>B cells</subject><subject>B-Lymphocytes - immunology</subject><subject>Bacillus Calmette-Guerin vaccine</subject><subject>BCG</subject><subject>Biology and Life Sciences</subject><subject>Care and treatment</subject><subject>CD19 antigen</subject><subject>CD27 antigen</subject><subject>Cell interactions</subject><subject>Cells, Cultured</subject><subject>Cytokines</subject><subject>Cytokines - biosynthesis</subject><subject>Dosage and administration</subject><subject>Humans</subject><subject>Humoral immunity</subject><subject>Immunity</subject><subject>Immunologic Memory</subject><subject>Immunological memory</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Interleukin 10</subject><subject>Interleukin 17</subject><subject>Interleukin 21</subject><subject>Latent Tuberculosis - immunology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lymphocytes B</subject><subject>Medicine and Health Sciences</subject><subject>Memory cells</subject><subject>Mycobacterium tuberculosis</subject><subject>Pathogens</subject><subject>Regulators</subject><subject>Stimulation</subject><subject>T cell receptors</subject><subject>TLR4 protein</subject><subject>TLR9 protein</subject><subject>Toll-Like Receptor 9 - physiology</subject><subject>Toll-like receptors</subject><subject>Tuberculosis</subject><subject>Tumor necrosis factor</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Vaccine development</subject><subject>Vaccines</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eJvO1wglYpBpUpDW-HWcly79ZTGne0g-Pc4NJsatIvJF7FOnvf18Tk-RfESginEHH649l1oVTPd-9ZMAaSIQ_CoOIUVRhOGAH58tD8pnsV4DQDFgrGnxQnigAIMxGmxXW1Ned61Ojmf3cpLE7NhNKW35edybpomlsmXsza5jWmdLq-S23WN6vGP5az8Hkzjdq5V4U_W7n1IvXKpkmlTuepqE3TX-Oji8-KJVU00L4bvWfHj_Mtq_m2yvPi6mM-WE80RTZMaEMgrpgWninLMMbNYU6wUZmsMCUSmIpjTGhOhsRZQcwuZIEpDZJXIsbPi9cF3n4-VQ5GihJxXAOMKoEwsDsTaq2u5D26Xk5deOfkv4MNGqpCcboysa2wFo4Sz2hLLRKWsqWu6VqBCGGGYvT4Np3X1zqx1vnVQzch0_Kd1W7nxvyQRCAmKs8G7wSD4m87EJHcu6lx21Rrf9XkLinDut3gAyishEGYko2_-Q-8vxEBtVL6ra63PKereVM4IxYQiwHpqeg-V19rsnM6Pz7ocHwnejwSZSeZ32qguRrm4unw4e_FzzL49YrdGNWkbfdP1TzGOQXIAdfAxBmPv-gGB7Gfnthqynx05zE6WvTru5Z3odljwX4omEf4</recordid><startdate>20160406</startdate><enddate>20160406</enddate><creator>du Plessis, Willem J</creator><creator>Kleynhans, Léanie</creator><creator>du Plessis, Nelita</creator><creator>Stanley, Kim</creator><creator>Malherbe, Stephanus T</creator><creator>Maasdorp, Elizna</creator><creator>Ronacher, Katharina</creator><creator>Chegou, Novel N</creator><creator>Walzl, Gerhard</creator><creator>Loxton, Andre G</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160406</creationdate><title>The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis</title><author>du Plessis, Willem J ; Kleynhans, Léanie ; du Plessis, Nelita ; Stanley, Kim ; Malherbe, Stephanus T ; Maasdorp, Elizna ; Ronacher, Katharina ; Chegou, Novel N ; Walzl, Gerhard ; Loxton, Andre G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-b041796c875a573736f3c53aa36d31412e94375b348c3c81c7f1684ac12fa88c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antigens</topic><topic>Antigens, Bacterial - immunology</topic><topic>B cells</topic><topic>B-Lymphocytes - immunology</topic><topic>Bacillus Calmette-Guerin vaccine</topic><topic>BCG</topic><topic>Biology and Life Sciences</topic><topic>Care and treatment</topic><topic>CD19 antigen</topic><topic>CD27 antigen</topic><topic>Cell interactions</topic><topic>Cells, Cultured</topic><topic>Cytokines</topic><topic>Cytokines - biosynthesis</topic><topic>Dosage and administration</topic><topic>Humans</topic><topic>Humoral immunity</topic><topic>Immunity</topic><topic>Immunologic Memory</topic><topic>Immunological memory</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Interleukin 10</topic><topic>Interleukin 17</topic><topic>Interleukin 21</topic><topic>Latent Tuberculosis - immunology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lymphocytes B</topic><topic>Medicine and Health Sciences</topic><topic>Memory cells</topic><topic>Mycobacterium tuberculosis</topic><topic>Pathogens</topic><topic>Regulators</topic><topic>Stimulation</topic><topic>T cell receptors</topic><topic>TLR4 protein</topic><topic>TLR9 protein</topic><topic>Toll-Like Receptor 9 - physiology</topic><topic>Toll-like receptors</topic><topic>Tuberculosis</topic><topic>Tumor necrosis factor</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Vaccine development</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>du Plessis, Willem J</creatorcontrib><creatorcontrib>Kleynhans, Léanie</creatorcontrib><creatorcontrib>du Plessis, Nelita</creatorcontrib><creatorcontrib>Stanley, Kim</creatorcontrib><creatorcontrib>Malherbe, Stephanus T</creatorcontrib><creatorcontrib>Maasdorp, Elizna</creatorcontrib><creatorcontrib>Ronacher, Katharina</creatorcontrib><creatorcontrib>Chegou, Novel N</creatorcontrib><creatorcontrib>Walzl, Gerhard</creatorcontrib><creatorcontrib>Loxton, Andre G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>du Plessis, Willem J</au><au>Kleynhans, Léanie</au><au>du Plessis, Nelita</au><au>Stanley, Kim</au><au>Malherbe, Stephanus T</au><au>Maasdorp, Elizna</au><au>Ronacher, Katharina</au><au>Chegou, Novel N</au><au>Walzl, Gerhard</au><au>Loxton, Andre G</au><au>Wilkinson, Katalin Andrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-04-06</date><risdate>2016</risdate><volume>11</volume><issue>4</issue><spage>e0152710</spage><epage>e0152710</epage><pages>e0152710-e0152710</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mycobacterium tuberculosis (M.tb) remains a successful pathogen, causing tuberculosis disease numbers to constantly increase. Although great progress has been made in delineating the disease, the host-pathogen interaction is incompletely described. B cells have shown to function as both effectors and regulators of immunity via non-humoral methods in both innate and adaptive immune settings. Here we assessed specific B cell functional interaction following stimulation with a broad range of antigens within the LTBI milieu. Our results indicate that B cells readily produce pro- and anti-inflammatory cytokines (including IL-1β, IL-10, IL-17, IL-21 and TNF-α) in response to stimulation. TLR4 and TLR9 based stimulations achieved the greatest secreted cytokine-production response and BCG stimulation displayed a clear preference for inducing IL-1β production. We also show that the cytokines produced by B cells are implicated strongly in cell-mediated communication and that plasma (memory) B cells (CD19+CD27+CD138+) is the subset with the greatest contribution to cytokine production. Collectively our data provides insight into B cell responses, where they are implicated in and quantifies responses from specific B cell phenotypes. These findings warrant further functional B cell research with a focus on specific B cell phenotypes under conditions of active TB disease to further our knowledge about the contribution of various cell subsets which could have implications for future vaccine development or refined B cell orientated treatment in the health setting.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27050308</pmid><doi>10.1371/journal.pone.0152710</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-04, Vol.11 (4), p.e0152710-e0152710 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1779033902 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Antigens Antigens, Bacterial - immunology B cells B-Lymphocytes - immunology Bacillus Calmette-Guerin vaccine BCG Biology and Life Sciences Care and treatment CD19 antigen CD27 antigen Cell interactions Cells, Cultured Cytokines Cytokines - biosynthesis Dosage and administration Humans Humoral immunity Immunity Immunologic Memory Immunological memory Inflammation Inflammation Mediators - metabolism Interleukin 10 Interleukin 17 Interleukin 21 Latent Tuberculosis - immunology Lipopolysaccharides - pharmacology Lymphocytes B Medicine and Health Sciences Memory cells Mycobacterium tuberculosis Pathogens Regulators Stimulation T cell receptors TLR4 protein TLR9 protein Toll-Like Receptor 9 - physiology Toll-like receptors Tuberculosis Tumor necrosis factor Tumor necrosis factor-TNF Tumor necrosis factor-α Vaccine development Vaccines |
| title | The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T04%3A10%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Functional%20Response%20of%20B%20Cells%20to%20Antigenic%20Stimulation:%20A%20Preliminary%20Report%20of%20Latent%20Tuberculosis&rft.jtitle=PloS%20one&rft.au=du%20Plessis,%20Willem%20J&rft.date=2016-04-06&rft.volume=11&rft.issue=4&rft.spage=e0152710&rft.epage=e0152710&rft.pages=e0152710-e0152710&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0152710&rft_dat=%3Cgale_plos_%3EA453452062%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1779033902&rft_id=info:pmid/27050308&rft_galeid=A453452062&rft_doaj_id=oai_doaj_org_article_bb3f865476bf4f689afebb5da0923231&rfr_iscdi=true |