Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements
Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing t...
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| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0152016-e0152016 |
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| creator | Mookerjee, Shona A Nicholls, David G Brand, Martin D |
| description | Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way. |
| doi_str_mv | 10.1371/journal.pone.0152016 |
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They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0152016</identifier><identifier>PMID: 27031845</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acidification ; Adenosine Triphosphate - metabolism ; Adherent cells ; Aging ; Animals ; Biochemistry - methods ; Bioenergetics ; Biology and Life Sciences ; Cancer ; Cell culture ; Cell Line ; Cell metabolism ; Cell Respiration ; Fibroblasts - metabolism ; Fluctuations ; Flux ; Genetic transformation ; Glycolysis ; Heart failure ; HEK293 Cells ; Humans ; Hyperoxia ; Metabolism ; Mice ; Myoblasts - metabolism ; Oxidative Phosphorylation ; Pharmacy ; Phosphorylation ; Physical Sciences ; Physiological aspects ; Respiration ; Sodium-Potassium-Exchanging ATPase - metabolism ; Stem cells ; Transformation</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0152016-e0152016</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Mookerjee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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| subjects | Acidification Adenosine Triphosphate - metabolism Adherent cells Aging Animals Biochemistry - methods Bioenergetics Biology and Life Sciences Cancer Cell culture Cell Line Cell metabolism Cell Respiration Fibroblasts - metabolism Fluctuations Flux Genetic transformation Glycolysis Heart failure HEK293 Cells Humans Hyperoxia Metabolism Mice Myoblasts - metabolism Oxidative Phosphorylation Pharmacy Phosphorylation Physical Sciences Physiological aspects Respiration Sodium-Potassium-Exchanging ATPase - metabolism Stem cells Transformation |
| title | Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements |
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