The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis
Ankyrin repeat and LEM-domain containing protein 1 (ANKLE1) is a GIY-YIG endonuclease with unknown functions, mainly expressed in mouse hematopoietic tissues. To test its potential role in hematopoiesis we generated Ankle1-deficient mice. Ankle1Δ/Δ mice are viable without any detectable phenotype in...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0152278-e0152278 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0152278 |
|---|---|
| container_issue | 3 |
| container_start_page | e0152278 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Braun, Juliane Meixner, Arabella Brachner, Andreas Foisner, Roland |
| description | Ankyrin repeat and LEM-domain containing protein 1 (ANKLE1) is a GIY-YIG endonuclease with unknown functions, mainly expressed in mouse hematopoietic tissues. To test its potential role in hematopoiesis we generated Ankle1-deficient mice. Ankle1Δ/Δ mice are viable without any detectable phenotype in hematopoiesis. Neither hematopoietic progenitor cells, myeloid and lymphoid progenitors, nor B and T cell development in bone marrow, spleen and thymus, are affected in Ankle1Δ/Δ-mice. Similarly embryonic stress erythropoiesis in liver and adult erythropoiesis in bone marrow and spleen appear normal. To test whether ANKLE1, like the only other known GIY-YIG endonuclease in mammals, SLX1, may contribute to Holliday junction resolution during DNA repair, Ankle1-deficient cells were exposed to various DNA-damage inducing agents. However, lack of Ankle1 did not affect cell viability and, unlike depletion of Slx1, Ankle1-deficiency did not increase sister chromatid exchange in Bloom helicase-depleted cells. Altogether, we show that lack of Ankle1 does neither affect mouse hematopoiesis nor DNA damage repair in mouse embryonic fibroblasts, indicating a redundant or non-essential function of ANKLE1 in mouse. |
| doi_str_mv | 10.1371/journal.pone.0152278 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1775627167</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A453470509</galeid><doaj_id>oai_doaj_org_article_a6c8ccfabf9449be87c576a5bd67ab1d</doaj_id><sourcerecordid>A453470509</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-1c21efbb432a0defb6944723edd11fef93cfc070ae131c3c48899fb26beb0f183</originalsourceid><addsrcrecordid>eNqNk0Fv0zAUxyMEYmPwDRBYQkLbIcWOkzi5IFVd6So6hsZA2slynOfWJbFDnCB646Pj0mxq0Q7IB1vPv_d_9t9-QfCS4BGhjLxb2741oho11sAIkySKWPYoOCY5jcI0wvTx3vooeObcGuOEZmn6NDiKGCY4wfQ4-H2zAjSb34a38xm62TSApqa0ppcVCAdobL5vWm3QNTQgOiRMiRbTS3Rua6FNOLGm87M2S_S5tR14kKDT8aePiyk5Q3OHzrVrwDhRVICUbdGl7b3oBdSis43V4LR7HjxRonLwYphPgq8fpjeTi3BxNZtPxotQpnnUhURGBFRRxDQSuPSrNI9jFlEoS0IUqJxKJTHDAgglkso4y_JcFVFaQIEVyehJ8Hqn21TW8cE8xwljSRoxkjJPzHdEacWaN62uRbvhVmj-N2DbJRdtp70zXKQyk1KJQvlT5AVkTCYsFUlRpkwUpPRa74dqfVFDKcF0ragORA93jF7xpf3J4wwzgnMvcDoItPZHD67jtXYSqkoY8CZuz53ijNGYevTNP-jDtxuopfAX0EZZX1duRfk4TmjM_H_Ylh09QPlRQq2l_2lK-_hBwtlBgmc6-NUtRe8cn3-5_n_26tsh-3aPXYGoupWzVd9pa9whGO9A2VrnWlD3JhPMt41y5wbfNgofGsWnvdp_oPuku86gfwAStw01</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1775627167</pqid></control><display><type>article</type><title>The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Braun, Juliane ; Meixner, Arabella ; Brachner, Andreas ; Foisner, Roland</creator><contributor>Zhou, Zhongjun</contributor><creatorcontrib>Braun, Juliane ; Meixner, Arabella ; Brachner, Andreas ; Foisner, Roland ; Zhou, Zhongjun</creatorcontrib><description>Ankyrin repeat and LEM-domain containing protein 1 (ANKLE1) is a GIY-YIG endonuclease with unknown functions, mainly expressed in mouse hematopoietic tissues. To test its potential role in hematopoiesis we generated Ankle1-deficient mice. Ankle1Δ/Δ mice are viable without any detectable phenotype in hematopoiesis. Neither hematopoietic progenitor cells, myeloid and lymphoid progenitors, nor B and T cell development in bone marrow, spleen and thymus, are affected in Ankle1Δ/Δ-mice. Similarly embryonic stress erythropoiesis in liver and adult erythropoiesis in bone marrow and spleen appear normal. To test whether ANKLE1, like the only other known GIY-YIG endonuclease in mammals, SLX1, may contribute to Holliday junction resolution during DNA repair, Ankle1-deficient cells were exposed to various DNA-damage inducing agents. However, lack of Ankle1 did not affect cell viability and, unlike depletion of Slx1, Ankle1-deficiency did not increase sister chromatid exchange in Bloom helicase-depleted cells. Altogether, we show that lack of Ankle1 does neither affect mouse hematopoiesis nor DNA damage repair in mouse embryonic fibroblasts, indicating a redundant or non-essential function of ANKLE1 in mouse.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0152278</identifier><identifier>PMID: 27010503</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal tissues ; Animals ; Ankyrins ; Biochemistry ; Biology and Life Sciences ; Bone marrow ; Breast cancer ; Cells (biology) ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA helicase ; DNA repair ; Embryo fibroblasts ; Embryos ; Endonuclease ; Endonucleases - genetics ; Endonucleases - physiology ; Erythropoiesis ; Fibroblasts ; Flow Cytometry ; Gene expression ; Gene Knockdown Techniques ; Genetic aspects ; Genomes ; Hematopoiesis ; Hematopoiesis - physiology ; Hematopoietic stem cells ; Laboratories ; Liver ; Lymphocytes B ; Lymphocytes T ; Mammals ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Nucleases ; Perutz, Max ; Physiological aspects ; Polymorphism, Single Nucleotide ; Progenitor cells ; Proteins ; Repair ; Research and Analysis Methods ; RNA, Messenger - genetics ; Rodents ; Sister chromatid exchange ; Spleen ; Stem cells ; Thymus</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0152278-e0152278</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Braun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Braun et al 2016 Braun et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-1c21efbb432a0defb6944723edd11fef93cfc070ae131c3c48899fb26beb0f183</citedby><cites>FETCH-LOGICAL-c692t-1c21efbb432a0defb6944723edd11fef93cfc070ae131c3c48899fb26beb0f183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4807109/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4807109/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27010503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhou, Zhongjun</contributor><creatorcontrib>Braun, Juliane</creatorcontrib><creatorcontrib>Meixner, Arabella</creatorcontrib><creatorcontrib>Brachner, Andreas</creatorcontrib><creatorcontrib>Foisner, Roland</creatorcontrib><title>The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Ankyrin repeat and LEM-domain containing protein 1 (ANKLE1) is a GIY-YIG endonuclease with unknown functions, mainly expressed in mouse hematopoietic tissues. To test its potential role in hematopoiesis we generated Ankle1-deficient mice. Ankle1Δ/Δ mice are viable without any detectable phenotype in hematopoiesis. Neither hematopoietic progenitor cells, myeloid and lymphoid progenitors, nor B and T cell development in bone marrow, spleen and thymus, are affected in Ankle1Δ/Δ-mice. Similarly embryonic stress erythropoiesis in liver and adult erythropoiesis in bone marrow and spleen appear normal. To test whether ANKLE1, like the only other known GIY-YIG endonuclease in mammals, SLX1, may contribute to Holliday junction resolution during DNA repair, Ankle1-deficient cells were exposed to various DNA-damage inducing agents. However, lack of Ankle1 did not affect cell viability and, unlike depletion of Slx1, Ankle1-deficiency did not increase sister chromatid exchange in Bloom helicase-depleted cells. Altogether, we show that lack of Ankle1 does neither affect mouse hematopoiesis nor DNA damage repair in mouse embryonic fibroblasts, indicating a redundant or non-essential function of ANKLE1 in mouse.</description><subject>Animal tissues</subject><subject>Animals</subject><subject>Ankyrins</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Bone marrow</subject><subject>Breast cancer</subject><subject>Cells (biology)</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA helicase</subject><subject>DNA repair</subject><subject>Embryo fibroblasts</subject><subject>Embryos</subject><subject>Endonuclease</subject><subject>Endonucleases - genetics</subject><subject>Endonucleases - physiology</subject><subject>Erythropoiesis</subject><subject>Fibroblasts</subject><subject>Flow Cytometry</subject><subject>Gene expression</subject><subject>Gene Knockdown Techniques</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Hematopoiesis</subject><subject>Hematopoiesis - physiology</subject><subject>Hematopoietic stem cells</subject><subject>Laboratories</subject><subject>Liver</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Mammals</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Nucleases</subject><subject>Perutz, Max</subject><subject>Physiological aspects</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Progenitor cells</subject><subject>Proteins</subject><subject>Repair</subject><subject>Research and Analysis Methods</subject><subject>RNA, Messenger - genetics</subject><subject>Rodents</subject><subject>Sister chromatid exchange</subject><subject>Spleen</subject><subject>Stem cells</subject><subject>Thymus</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk0Fv0zAUxyMEYmPwDRBYQkLbIcWOkzi5IFVd6So6hsZA2slynOfWJbFDnCB646Pj0mxq0Q7IB1vPv_d_9t9-QfCS4BGhjLxb2741oho11sAIkySKWPYoOCY5jcI0wvTx3vooeObcGuOEZmn6NDiKGCY4wfQ4-H2zAjSb34a38xm62TSApqa0ppcVCAdobL5vWm3QNTQgOiRMiRbTS3Rua6FNOLGm87M2S_S5tR14kKDT8aePiyk5Q3OHzrVrwDhRVICUbdGl7b3oBdSis43V4LR7HjxRonLwYphPgq8fpjeTi3BxNZtPxotQpnnUhURGBFRRxDQSuPSrNI9jFlEoS0IUqJxKJTHDAgglkso4y_JcFVFaQIEVyehJ8Hqn21TW8cE8xwljSRoxkjJPzHdEacWaN62uRbvhVmj-N2DbJRdtp70zXKQyk1KJQvlT5AVkTCYsFUlRpkwUpPRa74dqfVFDKcF0ragORA93jF7xpf3J4wwzgnMvcDoItPZHD67jtXYSqkoY8CZuz53ijNGYevTNP-jDtxuopfAX0EZZX1duRfk4TmjM_H_Ylh09QPlRQq2l_2lK-_hBwtlBgmc6-NUtRe8cn3-5_n_26tsh-3aPXYGoupWzVd9pa9whGO9A2VrnWlD3JhPMt41y5wbfNgofGsWnvdp_oPuku86gfwAStw01</recordid><startdate>20160324</startdate><enddate>20160324</enddate><creator>Braun, Juliane</creator><creator>Meixner, Arabella</creator><creator>Brachner, Andreas</creator><creator>Foisner, Roland</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160324</creationdate><title>The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis</title><author>Braun, Juliane ; Meixner, Arabella ; Brachner, Andreas ; Foisner, Roland</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-1c21efbb432a0defb6944723edd11fef93cfc070ae131c3c48899fb26beb0f183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animal tissues</topic><topic>Animals</topic><topic>Ankyrins</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Bone marrow</topic><topic>Breast cancer</topic><topic>Cells (biology)</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA helicase</topic><topic>DNA repair</topic><topic>Embryo fibroblasts</topic><topic>Embryos</topic><topic>Endonuclease</topic><topic>Endonucleases - genetics</topic><topic>Endonucleases - physiology</topic><topic>Erythropoiesis</topic><topic>Fibroblasts</topic><topic>Flow Cytometry</topic><topic>Gene expression</topic><topic>Gene Knockdown Techniques</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Hematopoiesis</topic><topic>Hematopoiesis - physiology</topic><topic>Hematopoietic stem cells</topic><topic>Laboratories</topic><topic>Liver</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Mammals</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Nucleases</topic><topic>Perutz, Max</topic><topic>Physiological aspects</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Progenitor cells</topic><topic>Proteins</topic><topic>Repair</topic><topic>Research and Analysis Methods</topic><topic>RNA, Messenger - genetics</topic><topic>Rodents</topic><topic>Sister chromatid exchange</topic><topic>Spleen</topic><topic>Stem cells</topic><topic>Thymus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Braun, Juliane</creatorcontrib><creatorcontrib>Meixner, Arabella</creatorcontrib><creatorcontrib>Brachner, Andreas</creatorcontrib><creatorcontrib>Foisner, Roland</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Braun, Juliane</au><au>Meixner, Arabella</au><au>Brachner, Andreas</au><au>Foisner, Roland</au><au>Zhou, Zhongjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-24</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0152278</spage><epage>e0152278</epage><pages>e0152278-e0152278</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Ankyrin repeat and LEM-domain containing protein 1 (ANKLE1) is a GIY-YIG endonuclease with unknown functions, mainly expressed in mouse hematopoietic tissues. To test its potential role in hematopoiesis we generated Ankle1-deficient mice. Ankle1Δ/Δ mice are viable without any detectable phenotype in hematopoiesis. Neither hematopoietic progenitor cells, myeloid and lymphoid progenitors, nor B and T cell development in bone marrow, spleen and thymus, are affected in Ankle1Δ/Δ-mice. Similarly embryonic stress erythropoiesis in liver and adult erythropoiesis in bone marrow and spleen appear normal. To test whether ANKLE1, like the only other known GIY-YIG endonuclease in mammals, SLX1, may contribute to Holliday junction resolution during DNA repair, Ankle1-deficient cells were exposed to various DNA-damage inducing agents. However, lack of Ankle1 did not affect cell viability and, unlike depletion of Slx1, Ankle1-deficiency did not increase sister chromatid exchange in Bloom helicase-depleted cells. Altogether, we show that lack of Ankle1 does neither affect mouse hematopoiesis nor DNA damage repair in mouse embryonic fibroblasts, indicating a redundant or non-essential function of ANKLE1 in mouse.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27010503</pmid><doi>10.1371/journal.pone.0152278</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0152278-e0152278 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1775627167 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animal tissues Animals Ankyrins Biochemistry Biology and Life Sciences Bone marrow Breast cancer Cells (biology) Deoxyribonucleic acid DNA DNA damage DNA helicase DNA repair Embryo fibroblasts Embryos Endonuclease Endonucleases - genetics Endonucleases - physiology Erythropoiesis Fibroblasts Flow Cytometry Gene expression Gene Knockdown Techniques Genetic aspects Genomes Hematopoiesis Hematopoiesis - physiology Hematopoietic stem cells Laboratories Liver Lymphocytes B Lymphocytes T Mammals Medicine and Health Sciences Mice Mice, Inbred C57BL Nucleases Perutz, Max Physiological aspects Polymorphism, Single Nucleotide Progenitor cells Proteins Repair Research and Analysis Methods RNA, Messenger - genetics Rodents Sister chromatid exchange Spleen Stem cells Thymus |
| title | The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T19%3A55%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20GIY-YIG%20Type%20Endonuclease%20Ankyrin%20Repeat%20and%20LEM%20Domain-Containing%20Protein%201%20(ANKLE1)%20Is%20Dispensable%20for%20Mouse%20Hematopoiesis&rft.jtitle=PloS%20one&rft.au=Braun,%20Juliane&rft.date=2016-03-24&rft.volume=11&rft.issue=3&rft.spage=e0152278&rft.epage=e0152278&rft.pages=e0152278-e0152278&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0152278&rft_dat=%3Cgale_plos_%3EA453470509%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1775627167&rft_id=info:pmid/27010503&rft_galeid=A453470509&rft_doaj_id=oai_doaj_org_article_a6c8ccfabf9449be87c576a5bd67ab1d&rfr_iscdi=true |