Insulin Resistance in PCOS Patients Enhances Oxidative Stress and Leukocyte Adhesion: Role of Myeloperoxidase
Cardiovascular diseases and oxidative stress are related to polycystic ovary syndrome (PCOS) and insulin resistance (IR). We have evaluated the relationship between myeloperoxidase (MPO) and leukocyte activation in PCOS patients according to homeostatic model assessment of IR (HOMA-IR), and have exp...
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| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0151960-e0151960 |
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| creator | Victor, Victor M Rovira-Llopis, Susana Bañuls, Celia Diaz-Morales, Noelia Martinez de Marañon, Arantxa Rios-Navarro, Cesar Alvarez, Angeles Gomez, Marcelino Rocha, Milagros Hernández-Mijares, Antonio |
| description | Cardiovascular diseases and oxidative stress are related to polycystic ovary syndrome (PCOS) and insulin resistance (IR). We have evaluated the relationship between myeloperoxidase (MPO) and leukocyte activation in PCOS patients according to homeostatic model assessment of IR (HOMA-IR), and have explored a possible correlation between these factors and endocrine and inflammatory parameters. This was a prospective controlled study conducted in an academic medical center. The study population consisted of 101 PCOS subjects and 105 control subjects. We divided PCOS subjects into PCOS non-IR (HOMA-IR2.5). Metabolic and anthropometric parameters, total and mitochondrial reactive oxygen species (ROS) production, MPO levels, interactions between human umbilical vein endothelial cells and leukocytes, adhesion molecules (E-selectin, ICAM-1 and VCAM-1) and proinflammatory cytokines (IL-6 and TNF-α) were evaluated. Oxidative stress was observed in PCOS patients, in whom there was an increase in total and mitochondrial ROS production and MPO levels. Enhanced rolling flux and adhesion, and a decrease in polymorphonuclear cell rolling velocity were also detected in PCOS subjects. Increases in IL-6 and TNF-α and adhesion molecules (E-selectin, ICAM-1 and VCAM-1) were also observed, particularly in the PCOS IR group, providing evidence that inflammation and oxidative stress are related in PCOS patients. HOMA-IR was positively correlated with hsCRP (p |
| doi_str_mv | 10.1371/journal.pone.0151960 |
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We have evaluated the relationship between myeloperoxidase (MPO) and leukocyte activation in PCOS patients according to homeostatic model assessment of IR (HOMA-IR), and have explored a possible correlation between these factors and endocrine and inflammatory parameters. This was a prospective controlled study conducted in an academic medical center. The study population consisted of 101 PCOS subjects and 105 control subjects. We divided PCOS subjects into PCOS non-IR (HOMA-IR<2.5) and PCOS IR (HOMA-IR>2.5). Metabolic and anthropometric parameters, total and mitochondrial reactive oxygen species (ROS) production, MPO levels, interactions between human umbilical vein endothelial cells and leukocytes, adhesion molecules (E-selectin, ICAM-1 and VCAM-1) and proinflammatory cytokines (IL-6 and TNF-α) were evaluated. Oxidative stress was observed in PCOS patients, in whom there was an increase in total and mitochondrial ROS production and MPO levels. Enhanced rolling flux and adhesion, and a decrease in polymorphonuclear cell rolling velocity were also detected in PCOS subjects. Increases in IL-6 and TNF-α and adhesion molecules (E-selectin, ICAM-1 and VCAM-1) were also observed, particularly in the PCOS IR group, providing evidence that inflammation and oxidative stress are related in PCOS patients. HOMA-IR was positively correlated with hsCRP (p<0.001, r = 0.304), ROS production (p<0.01, r = 0.593), leukocyte rolling flux (p<0.05, r = 0.446), E-selectin (p<0.01, r = 0.436) and IL-6 (p<0.001, r = 0.443). The results show an increase in the rate of ROS and MPO levels in PCOS patients in general, and particularly in those with IR. Inflammation in PCOS induces leukocyte-endothelium interactions and a simultaneous increase in IL-6, TNF-α, E-selectin, ICAM-1 and VCAM-1. These conditions are aggravated by the presence of IR.]]></description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0151960</identifier><identifier>PMID: 27007571</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adhesion ; Adult ; Age ; Analysis ; Anthropometry ; Atherosclerosis ; Biology and Life Sciences ; Biomedical research ; Cardiovascular disease ; Cardiovascular diseases ; Cell activation ; Cell Adhesion ; Cell adhesion molecules ; Cytokines ; Cytokines - metabolism ; Diabetes ; Diagnosis ; E-selectin ; Endocrinology ; Endothelial cells ; Endothelium ; Female ; Health care facilities ; Heart diseases ; Hospitals ; Humans ; Inflammation ; Inflammation Mediators - metabolism ; Insulin ; Insulin Resistance ; Intercellular adhesion molecule 1 ; Interleukin 6 ; Kinases ; Leukocyte rolling ; Leukocytes ; Leukocytes - cytology ; Medical research ; Medicine and Health Sciences ; Metabolic syndrome ; Mitochondria ; Mitochondria - metabolism ; Oxidation resistance ; Oxidative Stress ; Oxygen ; Patients ; Peroxidase ; Peroxidase - metabolism ; Pharmacology ; Physiological aspects ; Physiology ; Polycystic ovary syndrome ; Polycystic Ovary Syndrome - metabolism ; Polycystic Ovary Syndrome - pathology ; Polycystic Ovary Syndrome - physiopathology ; Population studies ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Research and Analysis Methods ; Risk factors ; Tumor necrosis factor-α ; Umbilical vein ; Vascular cell adhesion molecule 1 ; White blood cells ; Womens health ; Young Adult</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0151960-e0151960</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Victor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Victor et al 2016 Victor et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-5724c5a8ae55a1f38515a233ebb70df52674be20bad5faf3f19d9b3103ae3ffb3</citedby><cites>FETCH-LOGICAL-c758t-5724c5a8ae55a1f38515a233ebb70df52674be20bad5faf3f19d9b3103ae3ffb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805297/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805297/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27007571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ding, Wenjun</contributor><creatorcontrib>Victor, Victor M</creatorcontrib><creatorcontrib>Rovira-Llopis, Susana</creatorcontrib><creatorcontrib>Bañuls, Celia</creatorcontrib><creatorcontrib>Diaz-Morales, Noelia</creatorcontrib><creatorcontrib>Martinez de Marañon, Arantxa</creatorcontrib><creatorcontrib>Rios-Navarro, Cesar</creatorcontrib><creatorcontrib>Alvarez, Angeles</creatorcontrib><creatorcontrib>Gomez, Marcelino</creatorcontrib><creatorcontrib>Rocha, Milagros</creatorcontrib><creatorcontrib>Hernández-Mijares, Antonio</creatorcontrib><title>Insulin Resistance in PCOS Patients Enhances Oxidative Stress and Leukocyte Adhesion: Role of Myeloperoxidase</title><title>PloS one</title><addtitle>PLoS One</addtitle><description><![CDATA[Cardiovascular diseases and oxidative stress are related to polycystic ovary syndrome (PCOS) and insulin resistance (IR). We have evaluated the relationship between myeloperoxidase (MPO) and leukocyte activation in PCOS patients according to homeostatic model assessment of IR (HOMA-IR), and have explored a possible correlation between these factors and endocrine and inflammatory parameters. This was a prospective controlled study conducted in an academic medical center. The study population consisted of 101 PCOS subjects and 105 control subjects. We divided PCOS subjects into PCOS non-IR (HOMA-IR<2.5) and PCOS IR (HOMA-IR>2.5). Metabolic and anthropometric parameters, total and mitochondrial reactive oxygen species (ROS) production, MPO levels, interactions between human umbilical vein endothelial cells and leukocytes, adhesion molecules (E-selectin, ICAM-1 and VCAM-1) and proinflammatory cytokines (IL-6 and TNF-α) were evaluated. Oxidative stress was observed in PCOS patients, in whom there was an increase in total and mitochondrial ROS production and MPO levels. Enhanced rolling flux and adhesion, and a decrease in polymorphonuclear cell rolling velocity were also detected in PCOS subjects. Increases in IL-6 and TNF-α and adhesion molecules (E-selectin, ICAM-1 and VCAM-1) were also observed, particularly in the PCOS IR group, providing evidence that inflammation and oxidative stress are related in PCOS patients. HOMA-IR was positively correlated with hsCRP (p<0.001, r = 0.304), ROS production (p<0.01, r = 0.593), leukocyte rolling flux (p<0.05, r = 0.446), E-selectin (p<0.01, r = 0.436) and IL-6 (p<0.001, r = 0.443). The results show an increase in the rate of ROS and MPO levels in PCOS patients in general, and particularly in those with IR. Inflammation in PCOS induces leukocyte-endothelium interactions and a simultaneous increase in IL-6, TNF-α, E-selectin, ICAM-1 and VCAM-1. These conditions are aggravated by the presence of IR.]]></description><subject>Adhesion</subject><subject>Adult</subject><subject>Age</subject><subject>Analysis</subject><subject>Anthropometry</subject><subject>Atherosclerosis</subject><subject>Biology and Life Sciences</subject><subject>Biomedical research</subject><subject>Cardiovascular disease</subject><subject>Cardiovascular diseases</subject><subject>Cell activation</subject><subject>Cell Adhesion</subject><subject>Cell adhesion molecules</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Diabetes</subject><subject>Diagnosis</subject><subject>E-selectin</subject><subject>Endocrinology</subject><subject>Endothelial cells</subject><subject>Endothelium</subject><subject>Female</subject><subject>Health care facilities</subject><subject>Heart diseases</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Insulin</subject><subject>Insulin Resistance</subject><subject>Intercellular adhesion molecule 1</subject><subject>Interleukin 6</subject><subject>Kinases</subject><subject>Leukocyte rolling</subject><subject>Leukocytes</subject><subject>Leukocytes - cytology</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Metabolic syndrome</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Oxidation resistance</subject><subject>Oxidative Stress</subject><subject>Oxygen</subject><subject>Patients</subject><subject>Peroxidase</subject><subject>Peroxidase - metabolism</subject><subject>Pharmacology</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Polycystic ovary syndrome</subject><subject>Polycystic Ovary Syndrome - metabolism</subject><subject>Polycystic Ovary Syndrome - pathology</subject><subject>Polycystic Ovary Syndrome - physiopathology</subject><subject>Population studies</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Research and Analysis Methods</subject><subject>Risk factors</subject><subject>Tumor necrosis factor-α</subject><subject>Umbilical vein</subject><subject>Vascular cell adhesion molecule 1</subject><subject>White blood cells</subject><subject>Womens health</subject><subject>Young 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Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Victor, Victor M</au><au>Rovira-Llopis, Susana</au><au>Bañuls, Celia</au><au>Diaz-Morales, Noelia</au><au>Martinez de Marañon, Arantxa</au><au>Rios-Navarro, Cesar</au><au>Alvarez, Angeles</au><au>Gomez, Marcelino</au><au>Rocha, Milagros</au><au>Hernández-Mijares, Antonio</au><au>Ding, Wenjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin Resistance in PCOS Patients Enhances Oxidative Stress and Leukocyte Adhesion: Role of Myeloperoxidase</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-23</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0151960</spage><epage>e0151960</epage><pages>e0151960-e0151960</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract><![CDATA[Cardiovascular diseases and oxidative stress are related to polycystic ovary syndrome (PCOS) and insulin resistance (IR). We have evaluated the relationship between myeloperoxidase (MPO) and leukocyte activation in PCOS patients according to homeostatic model assessment of IR (HOMA-IR), and have explored a possible correlation between these factors and endocrine and inflammatory parameters. This was a prospective controlled study conducted in an academic medical center. The study population consisted of 101 PCOS subjects and 105 control subjects. We divided PCOS subjects into PCOS non-IR (HOMA-IR<2.5) and PCOS IR (HOMA-IR>2.5). Metabolic and anthropometric parameters, total and mitochondrial reactive oxygen species (ROS) production, MPO levels, interactions between human umbilical vein endothelial cells and leukocytes, adhesion molecules (E-selectin, ICAM-1 and VCAM-1) and proinflammatory cytokines (IL-6 and TNF-α) were evaluated. Oxidative stress was observed in PCOS patients, in whom there was an increase in total and mitochondrial ROS production and MPO levels. Enhanced rolling flux and adhesion, and a decrease in polymorphonuclear cell rolling velocity were also detected in PCOS subjects. Increases in IL-6 and TNF-α and adhesion molecules (E-selectin, ICAM-1 and VCAM-1) were also observed, particularly in the PCOS IR group, providing evidence that inflammation and oxidative stress are related in PCOS patients. HOMA-IR was positively correlated with hsCRP (p<0.001, r = 0.304), ROS production (p<0.01, r = 0.593), leukocyte rolling flux (p<0.05, r = 0.446), E-selectin (p<0.01, r = 0.436) and IL-6 (p<0.001, r = 0.443). The results show an increase in the rate of ROS and MPO levels in PCOS patients in general, and particularly in those with IR. Inflammation in PCOS induces leukocyte-endothelium interactions and a simultaneous increase in IL-6, TNF-α, E-selectin, ICAM-1 and VCAM-1. These conditions are aggravated by the presence of IR.]]></abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27007571</pmid><doi>10.1371/journal.pone.0151960</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0151960-e0151960 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1775367099 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adhesion Adult Age Analysis Anthropometry Atherosclerosis Biology and Life Sciences Biomedical research Cardiovascular disease Cardiovascular diseases Cell activation Cell Adhesion Cell adhesion molecules Cytokines Cytokines - metabolism Diabetes Diagnosis E-selectin Endocrinology Endothelial cells Endothelium Female Health care facilities Heart diseases Hospitals Humans Inflammation Inflammation Mediators - metabolism Insulin Insulin Resistance Intercellular adhesion molecule 1 Interleukin 6 Kinases Leukocyte rolling Leukocytes Leukocytes - cytology Medical research Medicine and Health Sciences Metabolic syndrome Mitochondria Mitochondria - metabolism Oxidation resistance Oxidative Stress Oxygen Patients Peroxidase Peroxidase - metabolism Pharmacology Physiological aspects Physiology Polycystic ovary syndrome Polycystic Ovary Syndrome - metabolism Polycystic Ovary Syndrome - pathology Polycystic Ovary Syndrome - physiopathology Population studies Reactive oxygen species Reactive Oxygen Species - metabolism Research and Analysis Methods Risk factors Tumor necrosis factor-α Umbilical vein Vascular cell adhesion molecule 1 White blood cells Womens health Young Adult |
| title | Insulin Resistance in PCOS Patients Enhances Oxidative Stress and Leukocyte Adhesion: Role of Myeloperoxidase |
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