Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo
Apolipoprotein A-II (ApoA-II) is down regulated in the sera of pancreatic ductal adenocarcinoma (PDAC) patients, which may be due to increase utilization of high density lipoprotein (HDL) lipid by pancreatic cancer tissue. This study examined the influence of exogenous ApoA-II on lipid uptake and ce...
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| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0151475-e0151475 |
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| creator | Julovi, Sohel M Xue, Aiqun Thanh LE, Thao N Gill, Anthony J Bulanadi, Jerikho C Patel, Mili Waddington, Lynne J Rye, Kerry-Anne Moghaddam, Minoo J Smith, Ross C |
| description | Apolipoprotein A-II (ApoA-II) is down regulated in the sera of pancreatic ductal adenocarcinoma (PDAC) patients, which may be due to increase utilization of high density lipoprotein (HDL) lipid by pancreatic cancer tissue. This study examined the influence of exogenous ApoA-II on lipid uptake and cell growth in pancreatic cancer (PC) both in vitro and in vivo.
Cryo transmission electron microscopy (TEM) examined ApoA-II's influence on morphology of SMOFLipid emulsion. The influence of ApoA-II on proliferation of cancer cell lines was determined by incubating them with lipid+/-ApoA-II and anti-SR-B1 antibody. Lipid was labeled with the fluorophore, DiD, to trace lipid uptake by cancer cells in vitro by confocal microscopy and in vivo in PDAC patient derived xenograft tumours (PDXT) by fluorescence imaging. Scavenger receptor class B type-1(SR-B1) expression in PDAC cell lines and in PDAC PDXT was measured by western blotting and immunohistochemistry, respectively.
ApoA-II spontaneously converted lipid emulsion into very small unilamellar rHDL like vesicles (rHDL/A-II) and enhanced lipid uptake in PANC-1, CFPAC-1 and primary tumour cells as shown by confocal microscopy. SR-B1 expression was 13.2, 10.6, 3.1 and 2.3 fold higher in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cell lines than the normal pancreatic cell line (HPDE6) and 3.7 fold greater in PDAC tissue than in normal pancreas. ApoA-II plus lipid significantly increased the uptake of labeled lipid and promoted cell growth in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cells which was inhibited by anti SR-B1 antibody. Further, ApoA-II increased the uptake of lipid in xenografts by 3.4 fold.
Our data suggest that ApoA-II enhance targeting potential of lipid in pancreatic cancer which may have imaging and drug delivery potentialities. |
| doi_str_mv | 10.1371/journal.pone.0151475 |
| format | Article |
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Cryo transmission electron microscopy (TEM) examined ApoA-II's influence on morphology of SMOFLipid emulsion. The influence of ApoA-II on proliferation of cancer cell lines was determined by incubating them with lipid+/-ApoA-II and anti-SR-B1 antibody. Lipid was labeled with the fluorophore, DiD, to trace lipid uptake by cancer cells in vitro by confocal microscopy and in vivo in PDAC patient derived xenograft tumours (PDXT) by fluorescence imaging. Scavenger receptor class B type-1(SR-B1) expression in PDAC cell lines and in PDAC PDXT was measured by western blotting and immunohistochemistry, respectively.
ApoA-II spontaneously converted lipid emulsion into very small unilamellar rHDL like vesicles (rHDL/A-II) and enhanced lipid uptake in PANC-1, CFPAC-1 and primary tumour cells as shown by confocal microscopy. SR-B1 expression was 13.2, 10.6, 3.1 and 2.3 fold higher in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cell lines than the normal pancreatic cell line (HPDE6) and 3.7 fold greater in PDAC tissue than in normal pancreas. ApoA-II plus lipid significantly increased the uptake of labeled lipid and promoted cell growth in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cells which was inhibited by anti SR-B1 antibody. Further, ApoA-II increased the uptake of lipid in xenografts by 3.4 fold.
Our data suggest that ApoA-II enhance targeting potential of lipid in pancreatic cancer which may have imaging and drug delivery potentialities.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0151475</identifier><identifier>PMID: 27002321</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenocarcinoma ; Analysis ; Apolipoprotein A ; Apolipoprotein A-II ; Apolipoprotein A-II - metabolism ; Apolipoproteins ; Biology and Life Sciences ; Biotechnology ; Breast cancer ; Cancer ; Cancer surgery ; Carcinoma, Pancreatic Ductal - metabolism ; Care and treatment ; Cell growth ; Cell Line, Tumor ; Cell Proliferation - physiology ; Cholesterol ; Complications and side effects ; Confocal microscopy ; Drug delivery ; Drug delivery systems ; Electron microscopy ; Epidermal growth factor ; Fluorescence ; High density lipoprotein ; Humans ; Immunohistochemistry ; In vitro methods and tests ; In vivo methods and tests ; Influence ; Lipids ; Lipids - physiology ; Lipoproteins ; Lipoproteins, HDL - metabolism ; Manufacturing ; MCF-7 Cells ; Medical research ; Medicine and Health Sciences ; Metabolism ; Ovarian cancer ; Pancreas ; Pancreatic cancer ; Pancreatic Neoplasms - metabolism ; Physical Sciences ; Prostate cancer ; Research and Analysis Methods ; Scavenger receptors ; Scavenger Receptors, Class B - metabolism ; Transmission electron microscopy ; Tumor cell lines ; Tumors ; Western blotting ; Xenografts</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0151475-e0151475</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Julovi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Julovi et al 2016 Julovi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c651t-a553dd9145a3cb3c6aae7aa66180fc09ef75615ecb05f504e7c2208a3839b09b3</citedby><cites>FETCH-LOGICAL-c651t-a553dd9145a3cb3c6aae7aa66180fc09ef75615ecb05f504e7c2208a3839b09b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803224/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803224/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27002321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Julovi, Sohel M</creatorcontrib><creatorcontrib>Xue, Aiqun</creatorcontrib><creatorcontrib>Thanh LE, Thao N</creatorcontrib><creatorcontrib>Gill, Anthony J</creatorcontrib><creatorcontrib>Bulanadi, Jerikho C</creatorcontrib><creatorcontrib>Patel, Mili</creatorcontrib><creatorcontrib>Waddington, Lynne J</creatorcontrib><creatorcontrib>Rye, Kerry-Anne</creatorcontrib><creatorcontrib>Moghaddam, Minoo J</creatorcontrib><creatorcontrib>Smith, Ross C</creatorcontrib><title>Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Apolipoprotein A-II (ApoA-II) is down regulated in the sera of pancreatic ductal adenocarcinoma (PDAC) patients, which may be due to increase utilization of high density lipoprotein (HDL) lipid by pancreatic cancer tissue. This study examined the influence of exogenous ApoA-II on lipid uptake and cell growth in pancreatic cancer (PC) both in vitro and in vivo.
Cryo transmission electron microscopy (TEM) examined ApoA-II's influence on morphology of SMOFLipid emulsion. The influence of ApoA-II on proliferation of cancer cell lines was determined by incubating them with lipid+/-ApoA-II and anti-SR-B1 antibody. Lipid was labeled with the fluorophore, DiD, to trace lipid uptake by cancer cells in vitro by confocal microscopy and in vivo in PDAC patient derived xenograft tumours (PDXT) by fluorescence imaging. Scavenger receptor class B type-1(SR-B1) expression in PDAC cell lines and in PDAC PDXT was measured by western blotting and immunohistochemistry, respectively.
ApoA-II spontaneously converted lipid emulsion into very small unilamellar rHDL like vesicles (rHDL/A-II) and enhanced lipid uptake in PANC-1, CFPAC-1 and primary tumour cells as shown by confocal microscopy. SR-B1 expression was 13.2, 10.6, 3.1 and 2.3 fold higher in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cell lines than the normal pancreatic cell line (HPDE6) and 3.7 fold greater in PDAC tissue than in normal pancreas. ApoA-II plus lipid significantly increased the uptake of labeled lipid and promoted cell growth in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cells which was inhibited by anti SR-B1 antibody. Further, ApoA-II increased the uptake of lipid in xenografts by 3.4 fold.
Our data suggest that ApoA-II enhance targeting potential of lipid in pancreatic cancer which may have imaging and drug delivery potentialities.</description><subject>Adenocarcinoma</subject><subject>Analysis</subject><subject>Apolipoprotein A</subject><subject>Apolipoprotein A-II</subject><subject>Apolipoprotein A-II - metabolism</subject><subject>Apolipoproteins</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cancer surgery</subject><subject>Carcinoma, Pancreatic Ductal - metabolism</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - physiology</subject><subject>Cholesterol</subject><subject>Complications and side effects</subject><subject>Confocal microscopy</subject><subject>Drug delivery</subject><subject>Drug delivery systems</subject><subject>Electron microscopy</subject><subject>Epidermal growth factor</subject><subject>Fluorescence</subject><subject>High density lipoprotein</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Influence</subject><subject>Lipids</subject><subject>Lipids - physiology</subject><subject>Lipoproteins</subject><subject>Lipoproteins, HDL - metabolism</subject><subject>Manufacturing</subject><subject>MCF-7 Cells</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Ovarian cancer</subject><subject>Pancreas</subject><subject>Pancreatic cancer</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Physical Sciences</subject><subject>Prostate cancer</subject><subject>Research and Analysis Methods</subject><subject>Scavenger receptors</subject><subject>Scavenger Receptors, Class B - metabolism</subject><subject>Transmission electron microscopy</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><subject>Western blotting</subject><subject>Xenografts</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptkl2LEzEUhgdR3HX1H4gGBPFmar5neiPUsq6Fgouu3oYzmbRNSZMxmeniv3emnV1aEQL5es6b856cLHtN8ISwgnzchi56cJMmeDPBRBBeiCfZJZkymkuK2dOT9UX2IqUtxoKVUj7PLmiBMWWUXGb3syY424QmhtZYj2b5YoFuXZfQ0ja2Rte7ziUbPLr2G_DaoLlxDt3EcN9u0N4C-vE9_0wQ-BrdQVybFt32WDTQWo3mQ0REC49-2TaGA3XY7MPL7NkKXDKvxvkq-_nl-m7-NV9-u1nMZ8tcS0HaHIRgdT0lXADTFdMSwBQAUpISrzSemlUhJBFGV1isBOam0JTiEljJphWeVuwqe3vUbVxIaqxZUqQoBKGFLHFPLI5EHWCrmmh3EP-oAFYdDkJcK4i9G2eUlhw4lwWrgPGaG-CUQklFPwgtZd1rfRpf66qdqbXxbQR3Jnp-4-1GrcNe8T4RSnkv8GEUiOF3Z1KrdjbpvuTgTeiOeUtGZTmg7_5B_-9upNbQG7B-Ffp39SCqZlwwXmApB-r9CbUx4NpNCq5r-59P5yA_gjqGlKJZPXojWA1t-ZCEGtpSjW3Zh705rctj0EMfsr8FzNyQ</recordid><startdate>20160322</startdate><enddate>20160322</enddate><creator>Julovi, Sohel M</creator><creator>Xue, Aiqun</creator><creator>Thanh LE, Thao N</creator><creator>Gill, Anthony J</creator><creator>Bulanadi, Jerikho C</creator><creator>Patel, Mili</creator><creator>Waddington, Lynne J</creator><creator>Rye, Kerry-Anne</creator><creator>Moghaddam, Minoo J</creator><creator>Smith, Ross C</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160322</creationdate><title>Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo</title><author>Julovi, Sohel M ; Xue, Aiqun ; Thanh LE, Thao N ; Gill, Anthony J ; Bulanadi, Jerikho C ; Patel, Mili ; Waddington, Lynne J ; Rye, Kerry-Anne ; Moghaddam, Minoo J ; Smith, Ross C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c651t-a553dd9145a3cb3c6aae7aa66180fc09ef75615ecb05f504e7c2208a3839b09b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adenocarcinoma</topic><topic>Analysis</topic><topic>Apolipoprotein A</topic><topic>Apolipoprotein A-II</topic><topic>Apolipoprotein A-II - metabolism</topic><topic>Apolipoproteins</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cancer surgery</topic><topic>Carcinoma, Pancreatic Ductal - metabolism</topic><topic>Care and treatment</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - physiology</topic><topic>Cholesterol</topic><topic>Complications and side effects</topic><topic>Confocal microscopy</topic><topic>Drug delivery</topic><topic>Drug delivery systems</topic><topic>Electron microscopy</topic><topic>Epidermal growth factor</topic><topic>Fluorescence</topic><topic>High density lipoprotein</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Influence</topic><topic>Lipids</topic><topic>Lipids - physiology</topic><topic>Lipoproteins</topic><topic>Lipoproteins, HDL - metabolism</topic><topic>Manufacturing</topic><topic>MCF-7 Cells</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Ovarian cancer</topic><topic>Pancreas</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Julovi, Sohel M</au><au>Xue, Aiqun</au><au>Thanh LE, Thao N</au><au>Gill, Anthony J</au><au>Bulanadi, Jerikho C</au><au>Patel, Mili</au><au>Waddington, Lynne J</au><au>Rye, Kerry-Anne</au><au>Moghaddam, Minoo J</au><au>Smith, Ross C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-22</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0151475</spage><epage>e0151475</epage><pages>e0151475-e0151475</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Apolipoprotein A-II (ApoA-II) is down regulated in the sera of pancreatic ductal adenocarcinoma (PDAC) patients, which may be due to increase utilization of high density lipoprotein (HDL) lipid by pancreatic cancer tissue. This study examined the influence of exogenous ApoA-II on lipid uptake and cell growth in pancreatic cancer (PC) both in vitro and in vivo.
Cryo transmission electron microscopy (TEM) examined ApoA-II's influence on morphology of SMOFLipid emulsion. The influence of ApoA-II on proliferation of cancer cell lines was determined by incubating them with lipid+/-ApoA-II and anti-SR-B1 antibody. Lipid was labeled with the fluorophore, DiD, to trace lipid uptake by cancer cells in vitro by confocal microscopy and in vivo in PDAC patient derived xenograft tumours (PDXT) by fluorescence imaging. Scavenger receptor class B type-1(SR-B1) expression in PDAC cell lines and in PDAC PDXT was measured by western blotting and immunohistochemistry, respectively.
ApoA-II spontaneously converted lipid emulsion into very small unilamellar rHDL like vesicles (rHDL/A-II) and enhanced lipid uptake in PANC-1, CFPAC-1 and primary tumour cells as shown by confocal microscopy. SR-B1 expression was 13.2, 10.6, 3.1 and 2.3 fold higher in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cell lines than the normal pancreatic cell line (HPDE6) and 3.7 fold greater in PDAC tissue than in normal pancreas. ApoA-II plus lipid significantly increased the uptake of labeled lipid and promoted cell growth in PANC-1, MIAPaCa-2, CFPAC-1 and BxPC3 cells which was inhibited by anti SR-B1 antibody. Further, ApoA-II increased the uptake of lipid in xenografts by 3.4 fold.
Our data suggest that ApoA-II enhance targeting potential of lipid in pancreatic cancer which may have imaging and drug delivery potentialities.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27002321</pmid><doi>10.1371/journal.pone.0151475</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0151475-e0151475 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1775127680 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adenocarcinoma Analysis Apolipoprotein A Apolipoprotein A-II Apolipoprotein A-II - metabolism Apolipoproteins Biology and Life Sciences Biotechnology Breast cancer Cancer Cancer surgery Carcinoma, Pancreatic Ductal - metabolism Care and treatment Cell growth Cell Line, Tumor Cell Proliferation - physiology Cholesterol Complications and side effects Confocal microscopy Drug delivery Drug delivery systems Electron microscopy Epidermal growth factor Fluorescence High density lipoprotein Humans Immunohistochemistry In vitro methods and tests In vivo methods and tests Influence Lipids Lipids - physiology Lipoproteins Lipoproteins, HDL - metabolism Manufacturing MCF-7 Cells Medical research Medicine and Health Sciences Metabolism Ovarian cancer Pancreas Pancreatic cancer Pancreatic Neoplasms - metabolism Physical Sciences Prostate cancer Research and Analysis Methods Scavenger receptors Scavenger Receptors, Class B - metabolism Transmission electron microscopy Tumor cell lines Tumors Western blotting Xenografts |
| title | Apolipoprotein A-II Plus Lipid Emulsion Enhance Cell Growth via SR-B1 and Target Pancreatic Cancer In Vitro and In Vivo |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A11%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Apolipoprotein%20A-II%20Plus%20Lipid%20Emulsion%20Enhance%20Cell%20Growth%20via%20SR-B1%20and%20Target%20Pancreatic%20Cancer%20In%20Vitro%20and%20In%20Vivo&rft.jtitle=PloS%20one&rft.au=Julovi,%20Sohel%20M&rft.date=2016-03-22&rft.volume=11&rft.issue=3&rft.spage=e0151475&rft.epage=e0151475&rft.pages=e0151475-e0151475&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0151475&rft_dat=%3Cgale_plos_%3EA453470660%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1775127680&rft_id=info:pmid/27002321&rft_galeid=A453470660&rft_doaj_id=oai_doaj_org_article_c64a44673ba34d4ea422a8258251286d&rfr_iscdi=true |