Metformin Decouples Phospholipid Metabolism in Breast Cancer Cells
The antidiabetic drug metformin, currently undergoing trials for cancer treatment, modulates lipid and glucose metabolism both crucial in phospholipid synthesis. Here the effect of treatment of breast tumour cells with metformin on phosphatidylcholine (PtdCho) metabolism which plays a key role in me...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0151179 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | e0151179 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Smith, Tim A D Phyu, Su M |
| description | The antidiabetic drug metformin, currently undergoing trials for cancer treatment, modulates lipid and glucose metabolism both crucial in phospholipid synthesis. Here the effect of treatment of breast tumour cells with metformin on phosphatidylcholine (PtdCho) metabolism which plays a key role in membrane synthesis and intracellular signalling has been examined.
MDA-MB-468, BT474 and SKBr3 breast cancer cell lines were treated with metformin and [3H-methyl]choline and [14C(U)]glucose incorporation and lipid accumulation determined in the presence and absence of lipase inhibitors. Activities of choline kinase (CK), CTP:phosphocholine cytidylyl transferase (CCT) and PtdCho-phospholipase C (PLC) were also measured. [3H] Radiolabelled metabolites were determined using thin layer chromatography.
Metformin-treated cells exhibited decreased formation of [3H]phosphocholine but increased accumulation of [3H]choline by PtdCho. CK and PLC activities were decreased and CCT activity increased by metformin-treatment. [14C] incorporation into fatty acids was decreased and into glycerol was increased in breast cancer cells treated with metformin incubated with [14C(U)]glucose.
This is the first study to show that treatment of breast cancer cells with metformin induces profound changes in phospholipid metabolism. |
| doi_str_mv | 10.1371/journal.pone.0151179 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1774177064</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A453531330</galeid><doaj_id>oai_doaj_org_article_041534cee79a4614affc22a202386752</doaj_id><sourcerecordid>A453531330</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-5198960334ddeee87e3c92a8fb95de4d9f90d2d86f9030082ec131b5f90baf23</originalsourceid><addsrcrecordid>eNqNkl2L1DAUhoso7rr6D0QLgujFjPlsmxthd_waWFnRxduQpqfTDGnTTVrRf29mprtMZS-khJykz3lPz-mbJM8xWmKa43dbN_pO2WXvOlgizDHOxYPkFAtKFhlB9OFRfJI8CWGLEKdFlj1OTkgmuGCInyYXX2GonW9Nl34A7cbeQki_NS70jbOmN1UaAVXGOLRphC48qDCkK9Vp8OkKrA1Pk0e1sgGeTftZcv3p4_Xqy-Ly6vN6dX650Jkgw4JjUYgMUcqqCgCKHKgWRBV1KXgFrBK1QBWpiizuFKGCgMYUlzweS1UTepa8PMj21gU5dR8kznMWF8pYJNYHonJqK3tvWuX_SKeM3F84v5HKD0ZbkIhhTpkGyIViGWaqrjUhiiASJ5TzXbX3U7WxbKHS0A1e2Zno_E1nGrlxvyTLCyYoigJvJgHvbkYIg2xN0HFeqgM37r-bFDQnlEf01T_o_d1N1EbFBkxXu1hX70TlOeNRBtN92eU9VHwqaI2OVqlNvJ8lvJ0lRGaA38NGjSHI9Y_v_89e_Zyzr4_YBpQdmuDsOBjXhTnIDqD2LgQP9d2QMZI7p99OQ-6cLienx7QXxz_oLunW2vQvYzf2rA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1774177064</pqid></control><display><type>article</type><title>Metformin Decouples Phospholipid Metabolism in Breast Cancer Cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Smith, Tim A D ; Phyu, Su M</creator><contributor>Shridhar, Viji</contributor><creatorcontrib>Smith, Tim A D ; Phyu, Su M ; Shridhar, Viji</creatorcontrib><description>The antidiabetic drug metformin, currently undergoing trials for cancer treatment, modulates lipid and glucose metabolism both crucial in phospholipid synthesis. Here the effect of treatment of breast tumour cells with metformin on phosphatidylcholine (PtdCho) metabolism which plays a key role in membrane synthesis and intracellular signalling has been examined.
MDA-MB-468, BT474 and SKBr3 breast cancer cell lines were treated with metformin and [3H-methyl]choline and [14C(U)]glucose incorporation and lipid accumulation determined in the presence and absence of lipase inhibitors. Activities of choline kinase (CK), CTP:phosphocholine cytidylyl transferase (CCT) and PtdCho-phospholipase C (PLC) were also measured. [3H] Radiolabelled metabolites were determined using thin layer chromatography.
Metformin-treated cells exhibited decreased formation of [3H]phosphocholine but increased accumulation of [3H]choline by PtdCho. CK and PLC activities were decreased and CCT activity increased by metformin-treatment. [14C] incorporation into fatty acids was decreased and into glycerol was increased in breast cancer cells treated with metformin incubated with [14C(U)]glucose.
This is the first study to show that treatment of breast cancer cells with metformin induces profound changes in phospholipid metabolism.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0151179</identifier><identifier>PMID: 26959405</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Analysis ; Antidiabetics ; Biology and Life Sciences ; Breast cancer ; Breast Neoplasms - metabolism ; Cancer ; Care and treatment ; Cell growth ; Cell Line, Tumor ; Cellular signal transduction ; Choline ; Choline - metabolism ; Choline kinase ; Chromatography ; CTP:phosphocholine cytidylyltransferase ; Diabetes mellitus ; Fatty acids ; Female ; Glucose ; Glucose metabolism ; Glycerol ; Humans ; Influence ; Intracellular signalling ; Kinases ; Lecithin ; Lipase ; Lipid metabolism ; Medicine and Health Sciences ; Metabolism ; Metabolites ; Metformin ; Metformin - pharmacology ; Phosphatidylcholine ; Phosphatidylcholines - metabolism ; Phospholipase ; Phospholipase C ; Phospholipids ; Phospholipids - metabolism ; Physical Sciences ; Physiological aspects ; Research and analysis methods ; Rodents ; Synthesis ; Thin layer chromatography ; Tumor cell lines ; Tumors</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0151179</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Smith, Phyu. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Smith, Phyu 2016 Smith, Phyu</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-5198960334ddeee87e3c92a8fb95de4d9f90d2d86f9030082ec131b5f90baf23</citedby><cites>FETCH-LOGICAL-c692t-5198960334ddeee87e3c92a8fb95de4d9f90d2d86f9030082ec131b5f90baf23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784930/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784930/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26959405$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Shridhar, Viji</contributor><creatorcontrib>Smith, Tim A D</creatorcontrib><creatorcontrib>Phyu, Su M</creatorcontrib><title>Metformin Decouples Phospholipid Metabolism in Breast Cancer Cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The antidiabetic drug metformin, currently undergoing trials for cancer treatment, modulates lipid and glucose metabolism both crucial in phospholipid synthesis. Here the effect of treatment of breast tumour cells with metformin on phosphatidylcholine (PtdCho) metabolism which plays a key role in membrane synthesis and intracellular signalling has been examined.
MDA-MB-468, BT474 and SKBr3 breast cancer cell lines were treated with metformin and [3H-methyl]choline and [14C(U)]glucose incorporation and lipid accumulation determined in the presence and absence of lipase inhibitors. Activities of choline kinase (CK), CTP:phosphocholine cytidylyl transferase (CCT) and PtdCho-phospholipase C (PLC) were also measured. [3H] Radiolabelled metabolites were determined using thin layer chromatography.
Metformin-treated cells exhibited decreased formation of [3H]phosphocholine but increased accumulation of [3H]choline by PtdCho. CK and PLC activities were decreased and CCT activity increased by metformin-treatment. [14C] incorporation into fatty acids was decreased and into glycerol was increased in breast cancer cells treated with metformin incubated with [14C(U)]glucose.
This is the first study to show that treatment of breast cancer cells with metformin induces profound changes in phospholipid metabolism.</description><subject>Accumulation</subject><subject>Analysis</subject><subject>Antidiabetics</subject><subject>Biology and Life Sciences</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - metabolism</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cellular signal transduction</subject><subject>Choline</subject><subject>Choline - metabolism</subject><subject>Choline kinase</subject><subject>Chromatography</subject><subject>CTP:phosphocholine cytidylyltransferase</subject><subject>Diabetes mellitus</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Glucose</subject><subject>Glucose metabolism</subject><subject>Glycerol</subject><subject>Humans</subject><subject>Influence</subject><subject>Intracellular signalling</subject><subject>Kinases</subject><subject>Lecithin</subject><subject>Lipase</subject><subject>Lipid metabolism</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metformin</subject><subject>Metformin - pharmacology</subject><subject>Phosphatidylcholine</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phospholipase</subject><subject>Phospholipase C</subject><subject>Phospholipids</subject><subject>Phospholipids - metabolism</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Research and analysis methods</subject><subject>Rodents</subject><subject>Synthesis</subject><subject>Thin layer chromatography</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLgujFjPlsmxthd_waWFnRxduQpqfTDGnTTVrRf29mprtMZS-khJykz3lPz-mbJM8xWmKa43dbN_pO2WXvOlgizDHOxYPkFAtKFhlB9OFRfJI8CWGLEKdFlj1OTkgmuGCInyYXX2GonW9Nl34A7cbeQki_NS70jbOmN1UaAVXGOLRphC48qDCkK9Vp8OkKrA1Pk0e1sgGeTftZcv3p4_Xqy-Ly6vN6dX650Jkgw4JjUYgMUcqqCgCKHKgWRBV1KXgFrBK1QBWpiizuFKGCgMYUlzweS1UTepa8PMj21gU5dR8kznMWF8pYJNYHonJqK3tvWuX_SKeM3F84v5HKD0ZbkIhhTpkGyIViGWaqrjUhiiASJ5TzXbX3U7WxbKHS0A1e2Zno_E1nGrlxvyTLCyYoigJvJgHvbkYIg2xN0HFeqgM37r-bFDQnlEf01T_o_d1N1EbFBkxXu1hX70TlOeNRBtN92eU9VHwqaI2OVqlNvJ8lvJ0lRGaA38NGjSHI9Y_v_89e_Zyzr4_YBpQdmuDsOBjXhTnIDqD2LgQP9d2QMZI7p99OQ-6cLienx7QXxz_oLunW2vQvYzf2rA</recordid><startdate>20160309</startdate><enddate>20160309</enddate><creator>Smith, Tim A D</creator><creator>Phyu, Su M</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160309</creationdate><title>Metformin Decouples Phospholipid Metabolism in Breast Cancer Cells</title><author>Smith, Tim A D ; Phyu, Su M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-5198960334ddeee87e3c92a8fb95de4d9f90d2d86f9030082ec131b5f90baf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Accumulation</topic><topic>Analysis</topic><topic>Antidiabetics</topic><topic>Biology and Life Sciences</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - metabolism</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cellular signal transduction</topic><topic>Choline</topic><topic>Choline - metabolism</topic><topic>Choline kinase</topic><topic>Chromatography</topic><topic>CTP:phosphocholine cytidylyltransferase</topic><topic>Diabetes mellitus</topic><topic>Fatty acids</topic><topic>Female</topic><topic>Glucose</topic><topic>Glucose metabolism</topic><topic>Glycerol</topic><topic>Humans</topic><topic>Influence</topic><topic>Intracellular signalling</topic><topic>Kinases</topic><topic>Lecithin</topic><topic>Lipase</topic><topic>Lipid metabolism</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metformin</topic><topic>Metformin - pharmacology</topic><topic>Phosphatidylcholine</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phospholipase</topic><topic>Phospholipase C</topic><topic>Phospholipids</topic><topic>Phospholipids - metabolism</topic><topic>Physical Sciences</topic><topic>Physiological aspects</topic><topic>Research and analysis methods</topic><topic>Rodents</topic><topic>Synthesis</topic><topic>Thin layer chromatography</topic><topic>Tumor cell lines</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Tim A D</creatorcontrib><creatorcontrib>Phyu, Su M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Tim A D</au><au>Phyu, Su M</au><au>Shridhar, Viji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metformin Decouples Phospholipid Metabolism in Breast Cancer Cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-09</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0151179</spage><pages>e0151179-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The antidiabetic drug metformin, currently undergoing trials for cancer treatment, modulates lipid and glucose metabolism both crucial in phospholipid synthesis. Here the effect of treatment of breast tumour cells with metformin on phosphatidylcholine (PtdCho) metabolism which plays a key role in membrane synthesis and intracellular signalling has been examined.
MDA-MB-468, BT474 and SKBr3 breast cancer cell lines were treated with metformin and [3H-methyl]choline and [14C(U)]glucose incorporation and lipid accumulation determined in the presence and absence of lipase inhibitors. Activities of choline kinase (CK), CTP:phosphocholine cytidylyl transferase (CCT) and PtdCho-phospholipase C (PLC) were also measured. [3H] Radiolabelled metabolites were determined using thin layer chromatography.
Metformin-treated cells exhibited decreased formation of [3H]phosphocholine but increased accumulation of [3H]choline by PtdCho. CK and PLC activities were decreased and CCT activity increased by metformin-treatment. [14C] incorporation into fatty acids was decreased and into glycerol was increased in breast cancer cells treated with metformin incubated with [14C(U)]glucose.
This is the first study to show that treatment of breast cancer cells with metformin induces profound changes in phospholipid metabolism.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26959405</pmid><doi>10.1371/journal.pone.0151179</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0151179 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1774177064 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Accumulation Analysis Antidiabetics Biology and Life Sciences Breast cancer Breast Neoplasms - metabolism Cancer Care and treatment Cell growth Cell Line, Tumor Cellular signal transduction Choline Choline - metabolism Choline kinase Chromatography CTP:phosphocholine cytidylyltransferase Diabetes mellitus Fatty acids Female Glucose Glucose metabolism Glycerol Humans Influence Intracellular signalling Kinases Lecithin Lipase Lipid metabolism Medicine and Health Sciences Metabolism Metabolites Metformin Metformin - pharmacology Phosphatidylcholine Phosphatidylcholines - metabolism Phospholipase Phospholipase C Phospholipids Phospholipids - metabolism Physical Sciences Physiological aspects Research and analysis methods Rodents Synthesis Thin layer chromatography Tumor cell lines Tumors |
| title | Metformin Decouples Phospholipid Metabolism in Breast Cancer Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T05%3A16%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Metformin%20Decouples%20Phospholipid%20Metabolism%20in%20Breast%20Cancer%20Cells&rft.jtitle=PloS%20one&rft.au=Smith,%20Tim%20A%20D&rft.date=2016-03-09&rft.volume=11&rft.issue=3&rft.spage=e0151179&rft.pages=e0151179-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0151179&rft_dat=%3Cgale_plos_%3EA453531330%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1774177064&rft_id=info:pmid/26959405&rft_galeid=A453531330&rft_doaj_id=oai_doaj_org_article_041534cee79a4614affc22a202386752&rfr_iscdi=true |